AT3G27250

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001258
Physical Location & Seq
Forward (+)
10061009 .. 10062870
1862 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27250.1

Sequence Viewer

Length: 849 bp
ATGGATCAAGATGACTGGTTGGGAACGCTAAGATACGCCGGTAAGGCGCAGGATAAAGTTTCCGTTGACGCTCTCATGCTCCGGTACCGTCCGATCGCTCCAAAGCCGACGACTGGTCAGCCATGTGGTGTGGCAGATAACAACAACAATAGCTCATACGGTATGAGCAAACGAACCAAACGAAAGTACGTTAGGGTTTCGAAGAATAATAAAGGCACGTGTCGAGGTAAGAGCAGATCTGACTTGTCTGATGATCGGGAACAAACTGATGTCGTGACGTTGCAACTCTTGCCGGAAAAATCAGATATTTCCGGTGAGTACTCGCCGTTGGATCAAGATAGTCTCGATCCGTCGGTGAAATCGATAATCGGAGAGGAAACACAAGAAACCAACACGTGGGGTATGTTTAACGGCAGCGTCACGGCGGAGATGGAGACGTGGGTGACGGTGGAGTCCGTCACAAGCGTGTGTGAGGGTAGTTTGAGTTCCCATGCGGTGGGGATTACGGACGTTGAGATCGTTGATAATCTTGGTAAGGACACGTGTCCAGCGTTCGTATCGGATGGCTCGAACCGTGTGGTGTGGGTCAACGAGGCTTACCGGAGAAATGTTTCCGGTGATGATTCAACGGCGTCAGTATCGCCGGAAGTTGTGGTGTGGTTGGTGGCGGAGGAGGCAACGGCGGCGATGCATTGTAACTACCAAGCTTTCACGTGTAGGGTGAGGATGCAATACACGTGGAAGGAAACAAAGTATACCAAAACGGTGCCGTGTGATGTGTGGAAAATGGAGTTTGGTGGTTTTGCATGGAGGCTAGACACAACAGCTGCTCTGACTCTCTGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

282

Amino Acids

31.35

Weight (kDa)

4.98

Isoelectric Point (pI)

32.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7950 PF25821 144 - 279 6.5e-52 Domain of unknown function (DUF7950)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 451
Acc65I GGTACC 1 cut(s) 84
AccB1I GGYRCC 2 cut(s) 84, 766
AccB7I CCANNNNNTGG 2 cut(s) 396, 496
AccI GTMKAC 1 cut(s) 755
AciI CCGC 4 cut(s) 425, 494, 668, 683
AclWI GGATC 3 cut(s) 12, 339, 341
AcvI CACGTG 5 cut(s) 219, 396, 543, 714, 738
AcyI GRCGYC 1 cut(s) 632
AfaI GTAC 3 cut(s) 86, 188, 320
AfiI CCNNNNNNNGG 3 cut(s) 113, 396, 496
AflIII ACRYGT 6 cut(s) 218, 393, 540, 542, 713, 735
AgsI TTSAA 1 cut(s) 627
AhdI GACNNNNNGTC 1 cut(s) 114
AjiI CACGTC 1 cut(s) 438
AleI CACNNNNGTG 1 cut(s) 464
AluBI AGCT 3 cut(s) 153, 707, 827
AluI AGCT 3 cut(s) 153, 707, 827
Alw26I GTCTC 2 cut(s) 347, 428
AlwI GGATC 3 cut(s) 12, 339, 341
ApeKI GCWGC 2 cut(s) 414, 827
Asp700I GAANNNNTTC 1 cut(s) 610
Asp718I GGTACC 1 cut(s) 84
AspLEI GCGC 1 cut(s) 49
AsuHPI GGTGA 5 cut(s) 326, 367, 454, 629, 733
AsuII TTCGAA 1 cut(s) 200
BaeI ACNNNNGTAYC 2 cut(s) 25, 58
BanI GGYRCC 2 cut(s) 84, 766
BbrPI CACGTG 5 cut(s) 219, 396, 543, 714, 738
BbvI GCAGC 2 cut(s) 426, 814
BccI CCATC 2 cut(s) 424, 557
BceAI ACGGC 6 cut(s) 310, 427, 438, 645, 696, 754
BcoDI GTCTC 2 cut(s) 347, 428
BfaI CTAG 1 cut(s) 815
BglI GCCNNNNNGGC 1 cut(s) 44
BglII AGATCT 1 cut(s) 236
BisI GCNGC 3 cut(s) 415, 684, 828
BlsI GCNGC 3 cut(s) 416, 685, 829
BmcAI AGTACT 1 cut(s) 320
BmeRI GACNNNNNGTC 1 cut(s) 114
BmgBI CACGTC 1 cut(s) 438
BmiI GGNNCC 2 cut(s) 86, 768
BmsI GCATC 2 cut(s) 678, 717
BoxI GACNNNNGTC 1 cut(s) 543
Bpu14I TTCGAA 1 cut(s) 200
Bsa29I ATCGAT 1 cut(s) 362
BsaAI YACGTR 5 cut(s) 219, 396, 543, 714, 738
BsaHI GRCGYC 1 cut(s) 632
BsaWI WCCGGW 4 cut(s) 81, 311, 600, 614
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 396, 496
Bse118I RCCGGY 1 cut(s) 38
Bse1I ACTGG 2 cut(s) 20, 118
BseCI ATCGAT 1 cut(s) 362
BseGI GGATG 2 cut(s) 568, 732
BseLI CCNNNNNNNGG 3 cut(s) 113, 396, 496
BseNI ACTGG 2 cut(s) 20, 118
BseRI GAGGAG 1 cut(s) 686
BseXI GCAGC 2 cut(s) 426, 814
Bsh1285I CGRYCG 1 cut(s) 96
BshNI GGYRCC 2 cut(s) 84, 766
BshVI ATCGAT 1 cut(s) 362
BsiEI CGRYCG 1 cut(s) 96
BsiSI CCGG 7 cut(s) 39, 82, 293, 312, 601, 615, 644
BslI CCNNNNNNNGG 3 cut(s) 113, 396, 496
BsmAI GTCTC 2 cut(s) 347, 428
BsmBI CGTCTC 1 cut(s) 428
Bsp119I TTCGAA 1 cut(s) 200
Bsp143I GATC 7 cut(s) 4, 93, 236, 253, 331, 346, 516
BspACI CCGC 4 cut(s) 425, 494, 668, 683
BspDI ATCGAT 1 cut(s) 362
BspLI GGNNCC 2 cut(s) 86, 768
BspPI GGATC 3 cut(s) 12, 339, 341
BspT104I TTCGAA 1 cut(s) 200
BspT107I GGYRCC 2 cut(s) 84, 766
BsrFI RCCGGY 1 cut(s) 38
BsrI ACTGG 2 cut(s) 20, 118
BssAI RCCGGY 1 cut(s) 38
BssMI GATC 7 cut(s) 4, 93, 236, 253, 331, 346, 516
BssNAI GTATAC 1 cut(s) 756
BssNI GRCGYC 1 cut(s) 632
Bst1107I GTATAC 1 cut(s) 756
Bst4CI ACNGT 5 cut(s) 89, 161, 448, 575, 766
BstACI GRCGYC 1 cut(s) 632
BstAPI GCANNNNNTGC 1 cut(s) 289
BstBAI YACGTR 5 cut(s) 219, 396, 543, 714, 738
BstBI TTCGAA 1 cut(s) 200
BstDEI CTNAG 1 cut(s) 29
BstF5I GGATG 2 cut(s) 568, 732
BstHHI GCGC 1 cut(s) 49
BstKTI GATC 7 cut(s) 7, 96, 239, 256, 334, 349, 519
BstMAI GTCTC 2 cut(s) 347, 428
BstMBI GATC 7 cut(s) 4, 93, 236, 253, 331, 346, 516
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 4 cut(s) 44, 289, 674, 683
BstPAI GACNNNNGTC 1 cut(s) 543
BstV1I GCAGC 2 cut(s) 426, 814
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
BstZ17I GTATAC 1 cut(s) 756
Bsu15I ATCGAT 1 cut(s) 362
BsuTUI ATCGAT 1 cut(s) 362
BtgZI GCGATG 1 cut(s) 701
BtrI CACGTC 1 cut(s) 438
BtsCI GGATG 2 cut(s) 568, 732
CfoI GCGC 1 cut(s) 49
Cfr10I RCCGGY 1 cut(s) 38
ClaI ATCGAT 1 cut(s) 362
CseI GACGC 3 cut(s) 77, 406, 621
Csp6I GTAC 3 cut(s) 85, 187, 319
CspCI CAANNNNNGTGG 2 cut(s) 719, 754
CviAII CATG 4 cut(s) 76, 123, 491, 807
CviJI RGCY 9 cut(s) 106, 121, 153, 567, 596, 707, 814, 827, 844
CviKI_1 RGCY 9 cut(s) 106, 121, 153, 567, 596, 707, 814, 827, 844
CviQI GTAC 3 cut(s) 85, 187, 319
DdeI CTNAG 1 cut(s) 29
DpnI GATC 7 cut(s) 6, 95, 238, 255, 333, 348, 518
DpnII GATC 7 cut(s) 4, 93, 236, 253, 331, 346, 516
DrdI GACNNNNNNGTC 1 cut(s) 451
DriI GACNNNNNGTC 1 cut(s) 114
DseDI GACNNNNNNGTC 1 cut(s) 451
Eam1105I GACNNNNNGTC 1 cut(s) 114
EciI GGCGGA 2 cut(s) 440, 683
Eco72I CACGTG 5 cut(s) 219, 396, 543, 714, 738
EcoT22I ATGCAT 1 cut(s) 693
Esp3I CGTCTC 1 cut(s) 428
FaeI CATG 4 cut(s) 79, 126, 494, 810
FaiI YATR 8 cut(s) 77, 124, 157, 164, 404, 492, 756, 808
FatI CATG 4 cut(s) 75, 122, 490, 806
FblI GTMKAC 1 cut(s) 755
Fnu4HI GCNGC 3 cut(s) 415, 684, 828
FokI GGATG 2 cut(s) 575, 739
Fsp4HI GCNGC 3 cut(s) 415, 684, 828
FspBI CTAG 1 cut(s) 815
GlaI GCGC 1 cut(s) 48
GluI GCNGC 3 cut(s) 415, 684, 828
HapII CCGG 7 cut(s) 39, 82, 293, 312, 601, 615, 644
HgaI GACGC 3 cut(s) 77, 406, 621
HhaI GCGC 1 cut(s) 49
Hin1I GRCGYC 1 cut(s) 632
Hin1II CATG 4 cut(s) 79, 126, 494, 810
Hin6I GCGC 1 cut(s) 47
HinP1I GCGC 1 cut(s) 47
HincII GTYRAC 2 cut(s) 67, 589
HindII GTYRAC 2 cut(s) 67, 589
HindIII AAGCTT 1 cut(s) 705
HinfI GANTC 3 cut(s) 452, 623, 835
HpaII CCGG 7 cut(s) 39, 82, 293, 312, 601, 615, 644
HphI GGTGA 5 cut(s) 326, 367, 454, 629, 733
Hpy166II GTNNAC 3 cut(s) 67, 589, 756
Hpy188I TCNGA 7 cut(s) 93, 241, 250, 304, 371, 562, 834
Hpy188III TCNNGA 5 cut(s) 8, 257, 274, 335, 344
Hpy8I GTNNAC 3 cut(s) 67, 589, 756
Hpy99I CGWCG 2 cut(s) 112, 355
HpyAV CCTTC 1 cut(s) 736
HpyCH4III ACNGT 5 cut(s) 89, 161, 448, 575, 766
HpyCH4IV ACGT 9 cut(s) 189, 218, 278, 395, 437, 510, 542, 713, 737
HpyCH4V TGCA 4 cut(s) 283, 691, 730, 806
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 289, 674, 683
HpyF3I CTNAG 1 cut(s) 29
HpySE526I ACGT 9 cut(s) 189, 218, 278, 395, 437, 510, 542, 713, 737
Hsp92I GRCGYC 1 cut(s) 632
Hsp92II CATG 4 cut(s) 79, 126, 494, 810
HspAI GCGC 1 cut(s) 47
KpnI GGTACC 1 cut(s) 88
Kzo9I GATC 7 cut(s) 4, 93, 236, 253, 331, 346, 516
LmnI GCTCC 2 cut(s) 84, 103
Lsp1109I GCAGC 2 cut(s) 426, 814
LweI GCATC 2 cut(s) 678, 717
MaeI CTAG 1 cut(s) 815
MaeII ACGT 9 cut(s) 189, 218, 278, 395, 437, 510, 542, 713, 737
MaeIII GTNAC 5 cut(s) 274, 418, 442, 457, 695
MalI GATC 7 cut(s) 6, 95, 238, 255, 333, 348, 518
MboI GATC 7 cut(s) 4, 93, 236, 253, 331, 346, 516
MboII GAAGA 1 cut(s) 214
MflI RGATCY 1 cut(s) 236
MlyI GAGTC 2 cut(s) 461, 829
MmeI TCCRAC 1 cut(s) 309
MnlI CCTC 8 cut(s) 218, 367, 466, 586, 664, 667, 717, 804
Mph1103I ATGCAT 1 cut(s) 693
MroXI GAANNNNTTC 1 cut(s) 610
MseI TTAA 1 cut(s) 408
MslI CAYNNNNRTG 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 827
MspI CCGG 7 cut(s) 39, 82, 293, 312, 601, 615, 644
MwoI GCNNNNNNNGC 4 cut(s) 44, 289, 674, 683
NdeII GATC 7 cut(s) 4, 93, 236, 253, 331, 346, 516
NlaIII CATG 4 cut(s) 79, 126, 494, 810
NlaIV GGNNCC 2 cut(s) 86, 768
NmuCI GTSAC 4 cut(s) 274, 418, 442, 457
NsiI ATGCAT 1 cut(s) 693
NspV TTCGAA 1 cut(s) 200
OliI CACNNNNGTG 1 cut(s) 464
PcsI WCGNNNNNNNCGW 5 cut(s) 178, 359, 516, 548, 566
PdmI GAANNNNTTC 1 cut(s) 610
PfeI GAWTC 1 cut(s) 623
PflMI CCANNNNNTGG 2 cut(s) 396, 496
PkrI GCNGC 3 cut(s) 416, 685, 829
Ple19I CGATCG 1 cut(s) 96
PleI GAGTC 2 cut(s) 460, 829
PmaCI CACGTG 5 cut(s) 219, 396, 543, 714, 738
PmlI CACGTG 5 cut(s) 219, 396, 543, 714, 738
PpsI GAGTC 2 cut(s) 460, 829
Ppu21I YACGTR 5 cut(s) 219, 396, 543, 714, 738
PshAI GACNNNNGTC 1 cut(s) 543
PspCI CACGTG 5 cut(s) 219, 396, 543, 714, 738
PspN4I GGNNCC 2 cut(s) 86, 768
PsrI GAACNNNNNNTAC 2 cut(s) 469, 501
PsuI RGATCY 1 cut(s) 236
PvuI CGATCG 1 cut(s) 96
PvuII CAGCTG 1 cut(s) 827
RsaI GTAC 3 cut(s) 86, 188, 320
RsaNI GTAC 3 cut(s) 85, 187, 319
RseI CAYNNNNRTG 1 cut(s) 464
SaqAI TTAA 1 cut(s) 408
SatI GCNGC 3 cut(s) 415, 684, 828
Sau3AI GATC 7 cut(s) 4, 93, 236, 253, 331, 346, 516
ScaI AGTACT 1 cut(s) 320
SchI GAGTC 2 cut(s) 461, 829
SfaNI GCATC 2 cut(s) 678, 717
SfuI TTCGAA 1 cut(s) 200
SmiMI CAYNNNNRTG 1 cut(s) 464
SsiI CCGC 4 cut(s) 425, 494, 668, 683
SspMI CTAG 1 cut(s) 815
TaaI ACNGT 5 cut(s) 89, 161, 448, 575, 766
TaiI ACGT 9 cut(s) 192, 221, 281, 398, 440, 513, 545, 716, 740
TaqI TCGA 5 cut(s) 200, 223, 345, 362, 569
TatI WGTACW 1 cut(s) 318
TauI GCSGC 1 cut(s) 686
TfiI GAWTC 1 cut(s) 623
Tru1I TTAA 1 cut(s) 408
Tru9I TTAA 1 cut(s) 408
TseFI GTSAC 4 cut(s) 274, 418, 442, 457
TseI GCWGC 2 cut(s) 414, 827
Tsp45I GTSAC 4 cut(s) 274, 418, 442, 457
TspGWI ACGGA 4 cut(s) 52, 339, 445, 521
Van91I CCANNNNNTGG 2 cut(s) 396, 496
XmiI GTMKAC 1 cut(s) 755
XmnI GAANNNNTTC 1 cut(s) 610
XspI CTAG 1 cut(s) 815
ZrmI AGTACT 1 cut(s) 320
Zsp2I ATGCAT 1 cut(s) 693
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.