MD09G1015800.v1.1

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001258
Physical Location & Seq
Forward (+)
974717 .. 979032
4316 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1015800.v1.1.491

Sequence Viewer

Length: 903 bp
ATGGAGGGCGGAGAACGGCGGCGGATGCTAAGAAGAGATGGTGGGGGGCAGGATGCGCCTGCAATCGTGAACCCGAACATGCTTAGATTTCGGCCGATTGCACCGAAACCGGTGACGAACAGAGCCGGAGAGGTTGGTAATAACGAAAACTCGGTTGCGAGTATTTTGACGAGTGCAAGAAGAAAGAGAAAGTACGTTAGGGTTTCCAAGAATAATGTATGTAGGGCGAAGAATAAGGATCCTCAGCGCGTGGTGACGACTCTGCAGCTCTTGCCGGAGAAAAACGATAACAACGGCTCATCGAACGGTGAATCTTGGCGTCCGTTAGATCCTACGGTGACGGACAATAATAACCATAGAAATAACAAAAATAGTAATCCTACTGGTAAAGAAGATGAGGGCTTAGGCCTAGGAATTCCCATGTGGTTGAGGAATTACATGGGTGGTGGAGGAGGATCAGATCCGACGGAGGGGATGCGGCAGATGGTGGCGGTGGAGTCTTGGGTGACTGTGGAGTGCGTGACAGGCACATGCATGAATGTGCAAGGTACGTTTGGAAGTTTTAGGAGTAGGGACGCCGAGAGGATGAAGAGTGATCTGGAGGGGGACACGTGTCCGGGTTTTATATCGGACGGTCAGAACAGGGTGCAGTGGTTGAACGGGGCGTTTAAGAGGATGGTGAGCCAGCAGAAGCGCGGGGCGGAGATAGCGGTGTGGTTGGTGGTGAAGGAGAAACTGCCGTACGCGGATGCTTCAGCGTTAATAACATGCCAAGTAAAGCTGCAGTACACGGTGGGTAAGGAGAGGTGCTCTCGAACTGTGCCTTGTGATTTGTGGAGAATGGACGATGGAGGATTTGCATGGAGGCTCGACGTTAACGCTGCTCTCACTCTGGGCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

301

Amino Acids

33.26

Weight (kDa)

9.57

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7950 PF25821 165 - 297 1.4e-50 Domain of unknown function (DUF7950)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 249, 696, 746
AciI CCGC 9 cut(s) 9, 19, 22, 478, 491, 696, 701, 710, 746
AclWI GGATC 5 cut(s) 233, 246, 323, 455, 463
AcoI YGGCCR 1 cut(s) 92
AcsI RAATTY 1 cut(s) 414
AcuI CTGAAG 1 cut(s) 738
AcvI CACGTG 1 cut(s) 612
AcyI GRCGYC 2 cut(s) 319, 576
AfaI GTAC 4 cut(s) 194, 550, 743, 788
AfiI CCNNNNNNNGG 1 cut(s) 470
AflIII ACRYGT 2 cut(s) 609, 611
AgeI ACCGGT 1 cut(s) 109
AgsI TTSAA 1 cut(s) 658
AluBI AGCT 2 cut(s) 268, 781
AluI AGCT 2 cut(s) 268, 781
Alw21I GWGCWC 1 cut(s) 812
AlwI GGATC 5 cut(s) 233, 246, 323, 455, 463
AoxI GGCC 3 cut(s) 92, 406, 895
ApeKI GCWGC 3 cut(s) 265, 781, 881
ApoI RAATTY 1 cut(s) 414
AsiGI ACCGGT 1 cut(s) 109
AspA2I CCTAGG 1 cut(s) 409
AspLEI GCGC 3 cut(s) 58, 249, 696
AspS9I GGNCC 1 cut(s) 895
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 7 cut(s) 124, 265, 320, 349, 517, 691, 736
AvrII CCTAGG 1 cut(s) 409
BamHI GGATCC 1 cut(s) 238
BbrPI CACGTG 1 cut(s) 612
Bbv12I GWGCWC 1 cut(s) 812
BbvCI CCTCAGC 1 cut(s) 243
BbvI GCAGC 3 cut(s) 277, 768, 868
BccI CCATC 4 cut(s) 32, 478, 670, 842
BceAI ACGGC 4 cut(s) 32, 310, 724, 882
BcnI CCSGG 1 cut(s) 618
BfaI CTAG 1 cut(s) 410
BfmI CTRYAG 2 cut(s) 263, 782
BisI GCNGC 5 cut(s) 20, 266, 479, 782, 882
BlnI CCTAGG 1 cut(s) 409
BlsI GCNGC 5 cut(s) 21, 267, 480, 783, 883
Bme1390I CCNGG 1 cut(s) 618
BmgT120I GGNCC 1 cut(s) 895
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 1 cut(s) 618
BmsI GCATC 4 cut(s) 15, 43, 465, 739
BoxI GACNNNNGTC 1 cut(s) 612
BplI GAGNNNNNCTC 4 cut(s) 794, 826, 796, 828
BpmI CTGGAG 1 cut(s) 620
Bpu10I CCTNAGC 2 cut(s) 243, 403
BpuMI CCSGG 1 cut(s) 618
BsaAI YACGTR 1 cut(s) 612
BsaHI GRCGYC 2 cut(s) 319, 576
BsaJI CCNNGG 1 cut(s) 409
BsaWI WCCGGW 1 cut(s) 109
Bsc4I CCNNNNNNNGG 1 cut(s) 470
Bse118I RCCGGY 1 cut(s) 109
Bse1I ACTGG 1 cut(s) 388
BseDI CCNNGG 1 cut(s) 409
BseGI GGATG 6 cut(s) 30, 58, 480, 591, 681, 754
BseLI CCNNNNNNNGG 1 cut(s) 470
BseMII CTCAG 1 cut(s) 257
BseNI ACTGG 1 cut(s) 388
BseRI GAGGAG 1 cut(s) 465
BseX3I CGGCCG 1 cut(s) 92
BseXI GCAGC 3 cut(s) 277, 768, 868
BsgI GTGCAG 1 cut(s) 668
Bsh1236I CGCG 3 cut(s) 249, 696, 746
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 3 cut(s) 94, 408, 897
BshTI ACCGGT 1 cut(s) 109
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 1 cut(s) 812
BsiSI CCGG 4 cut(s) 110, 126, 275, 617
BsiWI CGTACG 1 cut(s) 741
BslFI GGGAC 2 cut(s) 587, 620
BslI CCNNNNNNNGG 1 cut(s) 470
BsmFI GGGAC 2 cut(s) 587, 620
BsnI GGCC 3 cut(s) 94, 408, 897
Bsp1286I GDGCHC 1 cut(s) 812
Bsp143I GATC 5 cut(s) 238, 328, 455, 460, 595
BspACI CCGC 9 cut(s) 9, 19, 22, 478, 491, 696, 701, 710, 746
BspANI GGCC 3 cut(s) 94, 408, 897
BspCNI CTCAG 1 cut(s) 256
BspFNI CGCG 3 cut(s) 249, 696, 746
BspLI GGNNCC 1 cut(s) 240
BspMAI CTGCAG 2 cut(s) 267, 786
BspPI GGATC 5 cut(s) 233, 246, 323, 455, 463
BsrFI RCCGGY 1 cut(s) 109
BsrI ACTGG 1 cut(s) 388
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 1 cut(s) 409
BssMI GATC 5 cut(s) 238, 328, 455, 460, 595
BssNI GRCGYC 2 cut(s) 319, 576
BssT1I CCWWGG 1 cut(s) 409
Bst4CI ACNGT 6 cut(s) 308, 337, 511, 635, 793, 820
Bst6I CTCTTC 2 cut(s) 28, 584
BstACI GRCGYC 2 cut(s) 319, 576
BstAPI GCANNNNNTGC 1 cut(s) 271
BstBAI YACGTR 1 cut(s) 612
BstC8I GCNNGC 2 cut(s) 60, 686
BstDEI CTNAG 4 cut(s) 29, 83, 243, 403
BstF5I GGATG 6 cut(s) 30, 58, 480, 591, 681, 754
BstFNI CGCG 3 cut(s) 249, 696, 746
BstHHI GCGC 3 cut(s) 58, 249, 696
BstKTI GATC 5 cut(s) 241, 331, 458, 463, 598
BstMBI GATC 5 cut(s) 238, 328, 455, 460, 595
BstMCI CGRYCG 1 cut(s) 95
BstMWI GCNNNNNNNGC 5 cut(s) 25, 55, 271, 525, 707
BstNSI RCATGY 3 cut(s) 82, 534, 771
BstPAI GACNNNNGTC 1 cut(s) 612
BstSCI CCNGG 1 cut(s) 616
BstSFI CTRYAG 2 cut(s) 263, 782
BstUI CGCG 3 cut(s) 249, 696, 746
BstV1I GCAGC 3 cut(s) 277, 768, 868
BstX2I RGATCY 3 cut(s) 238, 328, 460
BstYI RGATCY 3 cut(s) 238, 328, 460
BstZI CGGCCG 1 cut(s) 92
BsuRI GGCC 3 cut(s) 94, 408, 897
BtsCI GGATG 6 cut(s) 30, 58, 480, 591, 681, 754
BtsI GCAGTG 1 cut(s) 656
BtsIMutI CAGTG 1 cut(s) 656
Cac8I GCNNGC 2 cut(s) 60, 686
CfoI GCGC 3 cut(s) 58, 249, 696
Cfr10I RCCGGY 1 cut(s) 109
Cfr13I GGNCC 1 cut(s) 895
CseI GACGC 2 cut(s) 308, 584
Csp6I GTAC 4 cut(s) 193, 549, 742, 787
CspAI ACCGGT 1 cut(s) 109
CviAII CATG 7 cut(s) 79, 421, 439, 531, 535, 768, 861
CviQI GTAC 4 cut(s) 193, 549, 742, 787
DdeI CTNAG 4 cut(s) 29, 83, 243, 403
DpnI GATC 5 cut(s) 240, 330, 457, 462, 597
DpnII GATC 5 cut(s) 238, 328, 455, 460, 595
EaeI YGGCCR 1 cut(s) 92
EagI CGGCCG 1 cut(s) 92
Eam1104I CTCTTC 2 cut(s) 28, 584
EarI CTCTTC 2 cut(s) 28, 584
EciI GGCGGA 3 cut(s) 24, 37, 716
EclXI CGGCCG 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 409
Eco147I AGGCCT 1 cut(s) 408
Eco52I CGGCCG 1 cut(s) 92
Eco57I CTGAAG 1 cut(s) 738
Eco72I CACGTG 1 cut(s) 612
EcoRI GAATTC 1 cut(s) 414
EcoT14I CCWWGG 1 cut(s) 409
EcoT22I ATGCAT 1 cut(s) 536
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 7 cut(s) 82, 424, 442, 534, 538, 771, 864
FaqI GGGAC 2 cut(s) 587, 620
FatI CATG 7 cut(s) 78, 420, 438, 530, 534, 767, 860
FauI CCCGC 1 cut(s) 689
Fnu4HI GCNGC 5 cut(s) 20, 266, 479, 782, 882
FokI GGATG 6 cut(s) 37, 65, 487, 598, 688, 761
Fsp4HI GCNGC 5 cut(s) 20, 266, 479, 782, 882
FspBI CTAG 1 cut(s) 410
GlaI GCGC 3 cut(s) 57, 248, 695
GluI GCNGC 5 cut(s) 20, 266, 479, 782, 882
GsuI CTGGAG 1 cut(s) 620
HaeIII GGCC 3 cut(s) 94, 408, 897
HapII CCGG 4 cut(s) 110, 126, 275, 617
HgaI GACGC 2 cut(s) 308, 584
HhaI GCGC 3 cut(s) 58, 249, 696
Hin1I GRCGYC 2 cut(s) 319, 576
Hin1II CATG 7 cut(s) 82, 424, 442, 534, 538, 771, 864
Hin6I GCGC 3 cut(s) 56, 247, 694
HinP1I GCGC 3 cut(s) 56, 247, 694
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HinfI GANTC 3 cut(s) 259, 311, 497
HpaI GTTAAC 1 cut(s) 877
HpaII CCGG 4 cut(s) 110, 126, 275, 617
HphI GGTGA 7 cut(s) 124, 265, 320, 349, 517, 691, 736
Hpy166II GTNNAC 3 cut(s) 70, 789, 877
Hpy188I TCNGA 4 cut(s) 460, 465, 631, 639
Hpy188III TCNNGA 3 cut(s) 67, 599, 813
Hpy8I GTNNAC 3 cut(s) 70, 789, 877
Hpy99I CGWCG 2 cut(s) 469, 875
HpyAV CCTTC 1 cut(s) 721
HpyCH4III ACNGT 6 cut(s) 308, 337, 511, 635, 793, 820
HpyCH4IV ACGT 4 cut(s) 195, 551, 611, 873
HpyCH4V TGCA 9 cut(s) 62, 101, 176, 265, 534, 544, 649, 784, 860
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 55, 271, 525, 707
HpyF3I CTNAG 4 cut(s) 29, 83, 243, 403
HpySE526I ACGT 4 cut(s) 195, 551, 611, 873
Hsp92I GRCGYC 2 cut(s) 319, 576
Hsp92II CATG 7 cut(s) 82, 424, 442, 534, 538, 771, 864
HspAI GCGC 3 cut(s) 56, 247, 694
KspAI GTTAAC 1 cut(s) 877
Kzo9I GATC 5 cut(s) 238, 328, 455, 460, 595
Lsp1109I GCAGC 3 cut(s) 277, 768, 868
LweI GCATC 4 cut(s) 15, 43, 465, 739
MaeI CTAG 1 cut(s) 410
MaeII ACGT 4 cut(s) 195, 551, 611, 873
MaeIII GTNAC 5 cut(s) 112, 253, 337, 505, 520
MalI GATC 5 cut(s) 240, 330, 457, 462, 597
MboI GATC 5 cut(s) 238, 328, 455, 460, 595
MboII GAAGA 5 cut(s) 45, 192, 241, 404, 601
MflI RGATCY 3 cut(s) 238, 328, 460
MhlI GDGCHC 1 cut(s) 812
MluCI AATT 2 cut(s) 414, 433
MlyI GAGTC 2 cut(s) 253, 506
MmeI TCCRAC 1 cut(s) 488
Mph1103I ATGCAT 1 cut(s) 536
MseI TTAA 3 cut(s) 669, 761, 876
MslI CAYNNNNRTG 2 cut(s) 533, 539
MspI CCGG 4 cut(s) 110, 126, 275, 617
MspR9I CCNGG 1 cut(s) 618
MvnI CGCG 3 cut(s) 249, 696, 746
MwoI GCNNNNNNNGC 5 cut(s) 25, 55, 271, 525, 707
NciI CCSGG 1 cut(s) 618
NdeII GATC 5 cut(s) 238, 328, 455, 460, 595
NlaIII CATG 7 cut(s) 82, 424, 442, 534, 538, 771, 864
NlaIV GGNNCC 1 cut(s) 240
NmeAIII GCCGAG 1 cut(s) 604
NmuCI GTSAC 5 cut(s) 112, 253, 337, 505, 520
NsiI ATGCAT 1 cut(s) 536
NspI RCATGY 3 cut(s) 82, 534, 771
PceI AGGCCT 1 cut(s) 408
PfeI GAWTC 1 cut(s) 311
Pfl23II CGTACG 1 cut(s) 741
PinAI ACCGGT 1 cut(s) 109
PkrI GCNGC 5 cut(s) 21, 267, 480, 783, 883
PleI GAGTC 2 cut(s) 253, 505
PmaCI CACGTG 1 cut(s) 612
PmlI CACGTG 1 cut(s) 612
PpsI GAGTC 2 cut(s) 253, 505
Ppu21I YACGTR 1 cut(s) 612
PshAI GACNNNNGTC 1 cut(s) 612
PspCI CACGTG 1 cut(s) 612
PspLI CGTACG 1 cut(s) 741
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 1 cut(s) 895
PstI CTGCAG 2 cut(s) 267, 786
PsuI RGATCY 3 cut(s) 238, 328, 460
RsaI GTAC 4 cut(s) 194, 550, 743, 788
RsaNI GTAC 4 cut(s) 193, 549, 742, 787
RseI CAYNNNNRTG 2 cut(s) 533, 539
SaqAI TTAA 3 cut(s) 669, 761, 876
SatI GCNGC 5 cut(s) 20, 266, 479, 782, 882
Sau3AI GATC 5 cut(s) 238, 328, 455, 460, 595
Sau96I GGNCC 1 cut(s) 895
SchI GAGTC 2 cut(s) 253, 506
ScrFI CCNGG 1 cut(s) 618
SduI GDGCHC 1 cut(s) 812
SetI ASST 9 cut(s) 135, 198, 270, 550, 554, 614, 783, 809, 876
SfaNI GCATC 4 cut(s) 15, 43, 465, 739
SfcI CTRYAG 2 cut(s) 263, 782
SmiMI CAYNNNNRTG 2 cut(s) 533, 539
Sse9I AATT 2 cut(s) 414, 433
SseBI AGGCCT 1 cut(s) 408
SsiI CCGC 9 cut(s) 9, 19, 22, 478, 491, 696, 701, 710, 746
SspMI CTAG 1 cut(s) 410
StuI AGGCCT 1 cut(s) 408
StyD4I CCNGG 1 cut(s) 616
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 6 cut(s) 308, 337, 511, 635, 793, 820
TaiI ACGT 4 cut(s) 198, 554, 614, 876
TaqI TCGA 3 cut(s) 302, 814, 870
TasI AATT 2 cut(s) 414, 433
TatI WGTACW 1 cut(s) 786
TauI GCSGC 2 cut(s) 22, 481
TfiI GAWTC 1 cut(s) 311
Tru1I TTAA 3 cut(s) 669, 761, 876
Tru9I TTAA 3 cut(s) 669, 761, 876
TscAI CASTG 1 cut(s) 656
TseFI GTSAC 5 cut(s) 112, 253, 337, 505, 520
TseI GCWGC 3 cut(s) 265, 781, 881
Tsp45I GTSAC 5 cut(s) 112, 253, 337, 505, 520
TspDTI ATGAA 2 cut(s) 551, 602
TspGWI ACGGA 3 cut(s) 312, 356, 482
TspRI CASTG 1 cut(s) 656
XapI RAATTY 1 cut(s) 414
XceI RCATGY 3 cut(s) 82, 534, 771
XmaJI CCTAGG 1 cut(s) 409
XspI CTAG 1 cut(s) 410
Zsp2I ATGCAT 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.