MD17G1016200.v1.1

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001258
Physical Location & Seq
Forward (+)
1307933 .. 1311930
3998 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1016200.v1.1.491

Sequence Viewer

Length: 897 bp
ATGGAAGGCGGAGAAGGGCGGCGCATGCTAAGATGCGCTGGGGGTGAGCAGGATAAGCATGCAATCATGAACCGGAACATGCTCAGATTCCGGCCGATTGCACCGAAACCGGTGGCCAACGGTGCCGGAGAGGTTGGTAACAAAAACTCGGGTTTGAGTGTTTTGACAAGTGCAAGAAGAAAGAGAAAGTACGTTAGGATTTCCAAGAATAATGTATGTAGGGCGAAGAATAAGGATCCTAAGCGCGTGGTGACGACGCTGCAGCTCTTGCCGGAGAAGAACGATATAGAGAGCTCGTCGGACAGTGGATCCGGGCGTCCTTTAGATCCTACGGTGACGGAAAGTAATAACGATAGAAATAATCAAAATAGTTATCCTGCTGGAAAAGAAGATGAGGGCTTACGCCTAGGAATTCCCATGTGGTTGAGGAATTACATGGGTGGTGGAGGAGGATCAGATCCGACGGTGGGTATGCGGCAGATGGTGGCGATGCAGTCTTGGGTGACGGTGGAGTGCGTGACAGGCACATGCATGAATGCGCACGGTACGTTTGGAAGTGTTAGGACTAGGGACGTGGAGGGGATGAGGAGTGATCTGGAGAGGGACACGTGTCCGGGGCTTATATCGGACGGTCAGAACAGGGTGCAGTGGCTGAACGAGGCTTTTAAGAAGATGGTGAGCCAGCAGAAGTGCGCGGCGGAGATAGCGGTGTGGTTGGTGGTGAAAGAGAAGCTGCCGTACGCGGACGCTTTAGCGTTAACGTGCCAAGTAAAGCTGCAGTACGCGGTGGGTAAGGAGAGGTGCTCTCGAATTGTGCCCTGTGATTTGTGGAGAATGGACGGTGGAGGATTTGCATGGAGGCTCGACGTCAAGGCTGCTCTAACTCTAGGCCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

299

Amino Acids

32.86

Weight (kDa)

9.52

Isoelectric Point (pI)

44.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7950 PF25821 164 - 295 1.3e-49 Domain of unknown function (DUF7950)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 870
Acc16I TGCGCA 1 cut(s) 540
AccB1I GGYRCC 1 cut(s) 122
AccII CGCG 4 cut(s) 246, 695, 743, 785
AciI CCGC 8 cut(s) 9, 19, 475, 695, 698, 707, 743, 785
AclWI GGATC 7 cut(s) 230, 243, 303, 316, 320, 452, 460
AcoI YGGCCR 2 cut(s) 92, 114
AcsI RAATTY 1 cut(s) 411
AcvI CACGTG 1 cut(s) 609
AcyI GRCGYC 2 cut(s) 316, 867
AfaI GTAC 4 cut(s) 191, 547, 740, 782
AfiI CCNNNNNNNGG 1 cut(s) 467
AflIII ACRYGT 2 cut(s) 606, 608
AgeI ACCGGT 1 cut(s) 109
AjiI CACGTC 1 cut(s) 574
AluBI AGCT 4 cut(s) 265, 294, 733, 775
AluI AGCT 4 cut(s) 265, 294, 733, 775
Alw21I GWGCWC 2 cut(s) 296, 806
AlwI GGATC 7 cut(s) 230, 243, 303, 316, 320, 452, 460
AlwNI CAGNNNCTG 1 cut(s) 652
Ama87I CYCGRG 1 cut(s) 148
AoxI GGCC 3 cut(s) 92, 114, 889
ApeKI GCWGC 5 cut(s) 259, 262, 733, 775, 875
ApoI RAATTY 1 cut(s) 411
AsiGI ACCGGT 1 cut(s) 109
AspA2I CCTAGG 1 cut(s) 406
AspLEI GCGC 5 cut(s) 24, 38, 246, 541, 695
AsuC2I CCSGG 2 cut(s) 313, 615
AsuHPI GGTGA 6 cut(s) 56, 262, 346, 514, 688, 733
AvaI CYCGRG 1 cut(s) 148
AvrII CCTAGG 1 cut(s) 406
BaeGI GKGCMC 1 cut(s) 819
BalI TGGCCA 1 cut(s) 116
BamHI GGATCC 2 cut(s) 235, 308
BanI GGYRCC 1 cut(s) 122
BanII GRGCYC 1 cut(s) 296
BarI GAAGNNNNNNTAC 2 cut(s) 722, 754
BbrPI CACGTG 1 cut(s) 609
Bbv12I GWGCWC 2 cut(s) 296, 806
BbvI GCAGC 5 cut(s) 246, 274, 720, 762, 862
BccI CCATC 2 cut(s) 475, 667
BceAI ACGGC 2 cut(s) 721, 876
BcnI CCSGG 2 cut(s) 313, 615
BfaI CTAG 3 cut(s) 407, 567, 887
BfmI CTRYAG 2 cut(s) 260, 776
BisI GCNGC 8 cut(s) 20, 260, 263, 476, 696, 734, 776, 876
BlnI CCTAGG 1 cut(s) 406
BlsI GCNGC 8 cut(s) 21, 261, 264, 477, 697, 735, 777, 877
Bme1390I CCNGG 2 cut(s) 313, 615
BmeT110I CYCGRG 1 cut(s) 148
BmgBI CACGTC 1 cut(s) 574
BmiI GGNNCC 3 cut(s) 124, 237, 310
BmrFI CCNGG 2 cut(s) 313, 615
BmsI GCATC 2 cut(s) 23, 480
BoxI GACNNNNGTC 1 cut(s) 609
BplI GAGNNNNNCTC 4 cut(s) 788, 820, 790, 822
BpmI CTGGAG 1 cut(s) 617
Bpu10I CCTNAGC 1 cut(s) 240
BpuMI CCSGG 2 cut(s) 313, 615
BsaAI YACGTR 1 cut(s) 609
BsaHI GRCGYC 2 cut(s) 316, 867
BsaJI CCNNGG 2 cut(s) 406, 614
BsaWI WCCGGW 2 cut(s) 72, 109
Bsc4I CCNNNNNNNGG 1 cut(s) 467
Bse118I RCCGGY 1 cut(s) 109
BseDI CCNNGG 2 cut(s) 406, 614
BseGI GGATG 1 cut(s) 588
BseLI CCNNNNNNNGG 1 cut(s) 467
BseMII CTCAG 1 cut(s) 97
BseRI GAGGAG 2 cut(s) 462, 601
BseSI GKGCMC 1 cut(s) 819
BseX3I CGGCCG 1 cut(s) 92
BseXI GCAGC 5 cut(s) 246, 274, 720, 762, 862
BseYI CCCAGC 1 cut(s) 38
BsgI GTGCAG 1 cut(s) 665
Bsh1236I CGCG 4 cut(s) 246, 695, 743, 785
Bsh1285I CGRYCG 1 cut(s) 95
BshFI GGCC 3 cut(s) 94, 116, 891
BshNI GGYRCC 1 cut(s) 122
BshTI ACCGGT 1 cut(s) 109
BsiEI CGRYCG 1 cut(s) 95
BsiHKAI GWGCWC 2 cut(s) 296, 806
BsiHKCI CYCGRG 1 cut(s) 148
BsiSI CCGG 7 cut(s) 73, 91, 110, 126, 272, 312, 614
BsiWI CGTACG 1 cut(s) 738
BslFI GGGAC 2 cut(s) 584, 617
BslI CCNNNNNNNGG 1 cut(s) 467
BsmFI GGGAC 2 cut(s) 584, 617
BsmI GAATGC 1 cut(s) 541
BsnI GGCC 3 cut(s) 94, 116, 891
BsoBI CYCGRG 1 cut(s) 148
Bsp1286I GDGCHC 3 cut(s) 296, 806, 819
Bsp143I GATC 6 cut(s) 235, 308, 325, 452, 457, 592
BspACI CCGC 8 cut(s) 9, 19, 475, 695, 698, 707, 743, 785
BspANI GGCC 3 cut(s) 94, 116, 891
BspCNI CTCAG 1 cut(s) 96
BspFNI CGCG 4 cut(s) 246, 695, 743, 785
BspHI TCATGA 1 cut(s) 66
BspLI GGNNCC 3 cut(s) 124, 237, 310
BspMAI CTGCAG 2 cut(s) 264, 780
BspPI GGATC 7 cut(s) 230, 243, 303, 316, 320, 452, 460
BspT107I GGYRCC 1 cut(s) 122
BsrFI RCCGGY 1 cut(s) 109
BssAI RCCGGY 1 cut(s) 109
BssECI CCNNGG 2 cut(s) 406, 614
BssMI GATC 6 cut(s) 235, 308, 325, 452, 457, 592
BssNI GRCGYC 2 cut(s) 316, 867
BssT1I CCWWGG 1 cut(s) 406
Bst4CI ACNGT 8 cut(s) 122, 305, 334, 466, 508, 545, 632, 842
BstACI GRCGYC 2 cut(s) 316, 867
BstAPI GCANNNNNTGC 1 cut(s) 268
BstBAI YACGTR 1 cut(s) 609
BstC8I GCNNGC 3 cut(s) 26, 60, 683
BstDEI CTNAG 3 cut(s) 29, 83, 240
BstF5I GGATG 1 cut(s) 588
BstFNI CGCG 4 cut(s) 246, 695, 743, 785
BstHHI GCGC 5 cut(s) 24, 38, 246, 541, 695
BstKTI GATC 6 cut(s) 238, 311, 328, 455, 460, 595
BstMBI GATC 6 cut(s) 235, 308, 325, 452, 457, 592
BstMCI CGRYCG 1 cut(s) 95
BstMWI GCNNNNNNNGC 6 cut(s) 25, 55, 122, 268, 522, 704
BstNSI RCATGY 4 cut(s) 28, 62, 82, 531
BstPAI GACNNNNGTC 1 cut(s) 609
BstSCI CCNGG 2 cut(s) 311, 613
BstSFI CTRYAG 2 cut(s) 260, 776
BstSLI GKGCMC 1 cut(s) 819
BstUI CGCG 4 cut(s) 246, 695, 743, 785
BstV1I GCAGC 5 cut(s) 246, 274, 720, 762, 862
BstX2I RGATCY 4 cut(s) 235, 308, 325, 457
BstYI RGATCY 4 cut(s) 235, 308, 325, 457
BstZI CGGCCG 1 cut(s) 92
BsuRI GGCC 3 cut(s) 94, 116, 891
BtgZI GCGATG 1 cut(s) 503
BtrI CACGTC 1 cut(s) 574
BtsCI GGATG 1 cut(s) 588
BtsI GCAGTG 1 cut(s) 653
BtsIMutI CAGTG 2 cut(s) 310, 653
Cac8I GCNNGC 3 cut(s) 26, 60, 683
CaiI CAGNNNCTG 1 cut(s) 652
CciI TCATGA 1 cut(s) 66
CfoI GCGC 5 cut(s) 24, 38, 246, 541, 695
Cfr10I RCCGGY 1 cut(s) 109
CseI GACGC 3 cut(s) 265, 305, 755
Csp6I GTAC 4 cut(s) 190, 546, 739, 781
CspAI ACCGGT 1 cut(s) 109
CviAII CATG 9 cut(s) 25, 59, 67, 79, 418, 436, 528, 532, 855
CviQI GTAC 4 cut(s) 190, 546, 739, 781
DdeI CTNAG 3 cut(s) 29, 83, 240
DpnI GATC 6 cut(s) 237, 310, 327, 454, 459, 594
DpnII GATC 6 cut(s) 235, 308, 325, 452, 457, 592
EaeI YGGCCR 2 cut(s) 92, 114
EagI CGGCCG 1 cut(s) 92
EciI GGCGGA 2 cut(s) 24, 713
Ecl136II GAGCTC 1 cut(s) 294
EclXI CGGCCG 1 cut(s) 92
Eco130I CCWWGG 1 cut(s) 406
Eco24I GRGCYC 1 cut(s) 296
Eco52I CGGCCG 1 cut(s) 92
Eco53kI GAGCTC 1 cut(s) 294
Eco72I CACGTG 1 cut(s) 609
Eco88I CYCGRG 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 294
EcoRI GAATTC 1 cut(s) 411
EcoT14I CCWWGG 1 cut(s) 406
EcoT22I ATGCAT 1 cut(s) 533
EcoT38I GRGCYC 1 cut(s) 296
ErhI CCWWGG 1 cut(s) 406
FaeI CATG 9 cut(s) 28, 62, 70, 82, 421, 439, 531, 535, 858
FaqI GGGAC 2 cut(s) 584, 617
FatI CATG 9 cut(s) 24, 58, 66, 78, 417, 435, 527, 531, 854
Fnu4HI GCNGC 8 cut(s) 20, 260, 263, 476, 696, 734, 776, 876
FokI GGATG 1 cut(s) 595
FriOI GRGCYC 1 cut(s) 296
Fsp4HI GCNGC 8 cut(s) 20, 260, 263, 476, 696, 734, 776, 876
FspAI RTGCGCAY 1 cut(s) 540
FspBI CTAG 3 cut(s) 407, 567, 887
FspI TGCGCA 1 cut(s) 540
GlaI GCGC 5 cut(s) 23, 37, 245, 540, 694
GluI GCNGC 8 cut(s) 20, 260, 263, 476, 696, 734, 776, 876
GsaI CCCAGC 1 cut(s) 42
GsuI CTGGAG 1 cut(s) 617
HaeIII GGCC 3 cut(s) 94, 116, 891
HapII CCGG 7 cut(s) 73, 91, 110, 126, 272, 312, 614
HgaI GACGC 3 cut(s) 265, 305, 755
HhaI GCGC 5 cut(s) 24, 38, 246, 541, 695
Hin1I GRCGYC 2 cut(s) 316, 867
Hin1II CATG 9 cut(s) 28, 62, 70, 82, 421, 439, 531, 535, 858
Hin6I GCGC 5 cut(s) 22, 36, 244, 539, 693
HinP1I GCGC 5 cut(s) 22, 36, 244, 539, 693
HincII GTYRAC 1 cut(s) 759
HindII GTYRAC 1 cut(s) 759
HinfI GANTC 1 cut(s) 87
HpaI GTTAAC 1 cut(s) 759
HpaII CCGG 7 cut(s) 73, 91, 110, 126, 272, 312, 614
HphI GGTGA 6 cut(s) 56, 262, 346, 514, 688, 733
Hpy166II GTNNAC 1 cut(s) 759
Hpy188I TCNGA 6 cut(s) 86, 301, 457, 462, 628, 636
Hpy188III TCNNGA 3 cut(s) 67, 596, 807
Hpy8I GTNNAC 1 cut(s) 759
Hpy99I CGWCG 4 cut(s) 259, 301, 466, 869
HpyAV CCTTC 1 cut(s) 8
HpyCH4III ACNGT 8 cut(s) 122, 305, 334, 466, 508, 545, 632, 842
HpyCH4IV ACGT 6 cut(s) 192, 548, 573, 608, 761, 867
HpyCH4V TGCA 9 cut(s) 62, 101, 173, 262, 493, 531, 646, 778, 854
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 55, 122, 268, 522, 704
HpyF3I CTNAG 3 cut(s) 29, 83, 240
HpySE526I ACGT 6 cut(s) 192, 548, 573, 608, 761, 867
Hsp92I GRCGYC 2 cut(s) 316, 867
Hsp92II CATG 9 cut(s) 28, 62, 70, 82, 421, 439, 531, 535, 858
HspAI GCGC 5 cut(s) 22, 36, 244, 539, 693
KspAI GTTAAC 1 cut(s) 759
Kzo9I GATC 6 cut(s) 235, 308, 325, 452, 457, 592
Lsp1109I GCAGC 5 cut(s) 246, 274, 720, 762, 862
LweI GCATC 2 cut(s) 23, 480
MaeI CTAG 3 cut(s) 407, 567, 887
MaeII ACGT 6 cut(s) 192, 548, 573, 608, 761, 867
MaeIII GTNAC 5 cut(s) 137, 250, 334, 502, 517
MalI GATC 6 cut(s) 237, 310, 327, 454, 459, 594
MboI GATC 6 cut(s) 235, 308, 325, 452, 457, 592
MboII GAAGA 5 cut(s) 189, 238, 289, 401, 682
MflI RGATCY 4 cut(s) 235, 308, 325, 457
MhlI GDGCHC 3 cut(s) 296, 806, 819
MlsI TGGCCA 1 cut(s) 116
MluCI AATT 3 cut(s) 411, 430, 810
MluNI TGGCCA 1 cut(s) 116
MmeI TCCRAC 2 cut(s) 279, 485
Mox20I TGGCCA 1 cut(s) 116
Mph1103I ATGCAT 1 cut(s) 533
MscI TGGCCA 1 cut(s) 116
MseI TTAA 2 cut(s) 666, 758
MslI CAYNNNNRTG 1 cut(s) 530
Msp20I TGGCCA 1 cut(s) 116
MspI CCGG 7 cut(s) 73, 91, 110, 126, 272, 312, 614
MspR9I CCNGG 2 cut(s) 313, 615
Mva1269I GAATGC 1 cut(s) 541
MvnI CGCG 4 cut(s) 246, 695, 743, 785
MwoI GCNNNNNNNGC 6 cut(s) 25, 55, 122, 268, 522, 704
NciI CCSGG 2 cut(s) 313, 615
NdeII GATC 6 cut(s) 235, 308, 325, 452, 457, 592
NlaIII CATG 9 cut(s) 28, 62, 70, 82, 421, 439, 531, 535, 858
NlaIV GGNNCC 3 cut(s) 124, 237, 310
NmuCI GTSAC 4 cut(s) 250, 334, 502, 517
NsbI TGCGCA 1 cut(s) 540
NsiI ATGCAT 1 cut(s) 533
NspI RCATGY 4 cut(s) 28, 62, 82, 531
PaeI GCATGC 2 cut(s) 28, 62
PagI TCATGA 1 cut(s) 66
PctI GAATGC 1 cut(s) 541
PfeI GAWTC 1 cut(s) 87
Pfl23II CGTACG 1 cut(s) 738
PinAI ACCGGT 1 cut(s) 109
PkrI GCNGC 8 cut(s) 21, 261, 264, 477, 697, 735, 777, 877
PmaCI CACGTG 1 cut(s) 609
PmlI CACGTG 1 cut(s) 609
Ppu21I YACGTR 1 cut(s) 609
PshAI GACNNNNGTC 1 cut(s) 609
Psp124BI GAGCTC 1 cut(s) 296
PspCI CACGTG 1 cut(s) 609
PspFI CCCAGC 1 cut(s) 38
PspLI CGTACG 1 cut(s) 738
PspN4I GGNNCC 3 cut(s) 124, 237, 310
PstI CTGCAG 2 cut(s) 264, 780
PstNI CAGNNNCTG 1 cut(s) 652
PsuI RGATCY 4 cut(s) 235, 308, 325, 457
RsaI GTAC 4 cut(s) 191, 547, 740, 782
RsaNI GTAC 4 cut(s) 190, 546, 739, 781
RseI CAYNNNNRTG 1 cut(s) 530
SacI GAGCTC 1 cut(s) 296
SaqAI TTAA 2 cut(s) 666, 758
SatI GCNGC 8 cut(s) 20, 260, 263, 476, 696, 734, 776, 876
Sau3AI GATC 6 cut(s) 235, 308, 325, 452, 457, 592
ScrFI CCNGG 2 cut(s) 313, 615
SduI GDGCHC 3 cut(s) 296, 806, 819
SfaNI GCATC 2 cut(s) 23, 480
SfcI CTRYAG 2 cut(s) 260, 776
SmiMI CAYNNNNRTG 1 cut(s) 530
SphI GCATGC 2 cut(s) 28, 62
Sse9I AATT 3 cut(s) 411, 430, 810
SsiI CCGC 8 cut(s) 9, 19, 475, 695, 698, 707, 743, 785
SspMI CTAG 3 cut(s) 407, 567, 887
SstI GAGCTC 1 cut(s) 296
StyD4I CCNGG 2 cut(s) 311, 613
StyI CCWWGG 1 cut(s) 406
TaaI ACNGT 8 cut(s) 122, 305, 334, 466, 508, 545, 632, 842
TaiI ACGT 6 cut(s) 195, 551, 576, 611, 764, 870
TaqI TCGA 2 cut(s) 808, 864
TasI AATT 3 cut(s) 411, 430, 810
TauI GCSGC 3 cut(s) 22, 478, 698
TfiI GAWTC 1 cut(s) 87
Tru1I TTAA 2 cut(s) 666, 758
Tru9I TTAA 2 cut(s) 666, 758
TscAI CASTG 2 cut(s) 310, 653
TseFI GTSAC 4 cut(s) 250, 334, 502, 517
TseI GCWGC 5 cut(s) 259, 262, 733, 775, 875
Tsp45I GTSAC 4 cut(s) 250, 334, 502, 517
TspDTI ATGAA 2 cut(s) 83, 548
TspGWI ACGGA 1 cut(s) 353
TspRI CASTG 2 cut(s) 310, 653
XapI RAATTY 1 cut(s) 411
XceI RCATGY 4 cut(s) 28, 62, 82, 531
XmaJI CCTAGG 1 cut(s) 406
XspI CTAG 3 cut(s) 407, 567, 887
ZraI GACGTC 1 cut(s) 868
Zsp2I ATGCAT 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.