AT3G56320

Nucleotidyltransferase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001135
Physical Location & Seq
Reverse (-)
20885894 .. 20890102
4209 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G56320.1

Sequence Viewer

Length: 1812 bp
ATGGGTGAAATTCAAGAAAGATTGTCAGTTTCATCATCTTCGTCTTCTTCATCATCCTCATTGTCACTATCAACGGCACTACCTAAAGGAGATTCATTGCCGATAGATGCAGATTCTTGGATGATTGCAGAGGAGAGAGCTCATGAGATACTTTGCACTATCCAACCTGCCCTTGTTTCAGACAGAAGTAGGAATGAGATCATTGATTATGTACGAACTCTTATCATGAGTCATGAGGGAATTGAGGTCTTTTCATTTGGTTCAGTGCCACTAAAAACCTATCTCCCGGACGGGGATATCGATCTGACTGTCCTAACTAAGCAAAATATGGATGACGATTTTTATGGACAATTGTGCAGTAGGCTTCAGAATGAAGAAAGAGAATCTGAATTCCATGCAACAGATGTTCAGTTTATCCCTGCACAGGTCAAGGTCATAAAATGCAATATCAGGAACATTGCTGTTGATATCTCTTTTAATCAAACGGCAGGTCTCTGTGCTTTATGTTTTCTGGAGCAGGTTGACCAACTCTTTGGGAGAGATCATCTTTTCAAGCGTAGCATCATCCTTGTCAAAGCTTGGTGCTATTACGAGAGTCGAATTCTTGGTGCTAACACTGGATTGATCTCAACATATGCATTGGCAGTACTAGTTTTGTACATCATCAACCTGTTTCATTCATCTTTATCTGGCCCTTTAGCGGTTCTGTATAAGTTCTTGGATTACTATGGTTCATTTGATTGGAACAACTACTGTATCAGTGTCAATGGTCCTGTGCCAATATCGTCCCTTCCAGAACTTACAGCAGCATCTCCAGAAAATGGGCATGAGTTATTACTTGACGAGAAGTTTTTAAGAAACTGTGTAGAGTTATATTCTGCTCCAACTAAAGCTGTTGACTCAAATGGTCTTGAGTTTCCTATCAAACATCTCAACATAGTTGATCCTCTAAAATACAGCAACAACCTCGGCAAAAGTGTTACACAAGGAAATGTCCAACGAATAAGACATGCTTTTACACTTGGAGCTCGAAAGCTAAGAGATGTTCTTTCACTACCTGGTGACACCATGGGATGGAGATTGGAGAAGTTCTTTCGTAACTCTTTGGAGAGAAATGGTAAGGGACAAAGGCAAGATGTGAATGATCCCGTTACTGCTTTCGGTACTGGAAGATCAGAATTATCTGAACTTAGTGGAGATTTCGATAGTTCCTTTGGAAGACTTGTGTATGGGCAAATGTATCATGGGCATAGCTTACCTGGAACATTTCAGCATGGCTATATCCCGGTTAGTTCTCACCTGAGTGGTTGGGATATTGTTCGTCACCTTGTGACTTACCGAAAGAACGAGTTCCATCTTAGAAGTCTCAATGTATCAACCTCTATGCAGCCTTTTCCTTTGCATTCTTTACCTAATGGCTGTCAAAACATGAGAAAGACGAGAGGAACCGGCACTTATATTCCTGACATGAGTCAACAGGTATACTCTGACAGGTTCAGAGAATCAGGTACTGGAACCTCATCAACGAATCATTTAGAAGCTTCCTCTGAGGCAATAGACAATGATGGAGCCAGTAGTTGCTGCAATCTTTCCGGTGAAGTGTCTACTTCTTGTACCAGTAACAAAGGAGAAGAATGTGTGAGACCAGAGAGTGTGCTGAAATCTCAAAGATCTGCGAATTTGGAGCTTGATGACAATTGCCAATTTCCACCACCAGAAAACCTAGTAGAACCTAGAACTTCAAGCACATTGGTATTGGAAAATGGCAAAGAAGAGAGTATCAGATCCTCCCAAACCATGAACGGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2 PAP_assoc

Protein Analysis

603

Amino Acids

66.96

Weight (kDa)

5.45

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 48 - 165 9e-08 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
PAP-OAS1 PF26180 179 - 369 5.3e-87 PAP/OAS1 substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 175, 479, 508
AccB7I CCANNNNNTGG 2 cut(s) 821, 1074
AccI GTMKAC 2 cut(s) 1482, 1604
AciI CCGC 1 cut(s) 701
AclWI GGATC 3 cut(s) 938, 1139, 1779
AcsI RAATTY 4 cut(s) 9, 389, 600, 1678
AcuI CTGAAG 1 cut(s) 350
AdeI CACNNNGTG 1 cut(s) 1330
AfaI GTAC 6 cut(s) 213, 648, 659, 1165, 1510, 1615
AfiI CCNNNNNNNGG 5 cut(s) 292, 424, 700, 821, 1074
AgsI TTSAA 3 cut(s) 14, 553, 1743
AhlI ACTAGT 1 cut(s) 649
AjnI CCWGG 2 cut(s) 1057, 1258
AleI CACNNNNGTG 1 cut(s) 1302
AluBI AGCT 8 cut(s) 140, 578, 893, 1028, 1036, 1254, 1541, 1687
AluI AGCT 8 cut(s) 140, 578, 893, 1028, 1036, 1254, 1541, 1687
Alw21I GWGCWC 2 cut(s) 142, 1030
Alw26I GTCTC 3 cut(s) 497, 1370, 1636
AlwI GGATC 3 cut(s) 938, 1139, 1779
AlwNI CAGNNNCTG 1 cut(s) 1511
AoxI GGCC 1 cut(s) 691
ApeKI GCWGC 3 cut(s) 806, 1387, 1581
ApoI RAATTY 4 cut(s) 9, 389, 600, 1678
ArsI GACNNNNNNTTYG 4 cut(s) 475, 507, 515, 547
Asp700I GAANNNNTTC 1 cut(s) 1349
AspS9I GGNCC 2 cut(s) 692, 770
AsuC2I CCSGG 2 cut(s) 287, 1286
AsuHPI GGTGA 5 cut(s) 17, 1073, 1289, 1316, 1607
AvaII GGWCC 1 cut(s) 770
BaeI ACNNNNGTAYC 2 cut(s) 739, 772
BanII GRGCYC 2 cut(s) 142, 1030
BbsI GAAGAC 2 cut(s) 36, 1225
Bbv12I GWGCWC 2 cut(s) 142, 1030
BbvI GCAGC 3 cut(s) 818, 1399, 1568
BccI CCATC 3 cut(s) 1068, 1362, 1559
BceAI ACGGC 2 cut(s) 90, 501
BcgI CGANNNNNNTGC 2 cut(s) 949, 983
BciT130I CCWGG 2 cut(s) 1059, 1260
BcnI CCSGG 2 cut(s) 287, 1286
BcoDI GTCTC 3 cut(s) 497, 1370, 1636
BcuI ACTAGT 1 cut(s) 649
BfaI CTAG 3 cut(s) 650, 1724, 1734
BfuAI ACCTGC 3 cut(s) 175, 479, 508
BglII AGATCT 1 cut(s) 1670
BisI GCNGC 3 cut(s) 807, 1388, 1582
BlsI GCNGC 3 cut(s) 808, 1389, 1583
BmcAI AGTACT 1 cut(s) 648
Bme1390I CCNGG 4 cut(s) 287, 1059, 1260, 1286
Bme18I GGWCC 1 cut(s) 770
BmgT120I GGNCC 2 cut(s) 692, 770
BmiI GGNNCC 3 cut(s) 1447, 1516, 1570
BmrFI CCNGG 4 cut(s) 287, 1059, 1260, 1286
BmsI GCATC 3 cut(s) 97, 570, 818
BoxI GACNNNNGTC 1 cut(s) 1470
BpiI GAAGAC 2 cut(s) 36, 1225
BpmI CTGGAG 2 cut(s) 533, 798
BpuEI CTTGAG 1 cut(s) 932
BpuMI CCSGG 2 cut(s) 287, 1286
Bsa29I ATCGAT 1 cut(s) 300
BsaBI GATNNNNATC 1 cut(s) 300
BsaI GGTCTC 2 cut(s) 497, 1636
BsaJI CCNNGG 2 cut(s) 967, 1068
BsaWI WCCGGW 1 cut(s) 1592
Bsc4I CCNNNNNNNGG 5 cut(s) 292, 424, 700, 821, 1074
Bse118I RCCGGY 1 cut(s) 1448
Bse1I ACTGG 5 cut(s) 622, 1171, 1516, 1572, 1617
Bse3DI GCAATG 2 cut(s) 95, 456
Bse8I GATNNNNATC 1 cut(s) 300
BseBI CCWGG 2 cut(s) 1059, 1260
BseCI ATCGAT 1 cut(s) 300
BseDI CCNNGG 2 cut(s) 967, 1068
BseGI GGATG 5 cut(s) 53, 126, 337, 564, 1079
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 5 cut(s) 292, 424, 700, 821, 1074
BseMI GCAATG 2 cut(s) 95, 456
BseMII CTCAG 2 cut(s) 1292, 1539
BseNI ACTGG 5 cut(s) 622, 1171, 1516, 1572, 1617
BseRI GAGGAG 1 cut(s) 146
BseXI GCAGC 3 cut(s) 818, 1399, 1568
BsgI GTGCAG 2 cut(s) 376, 405
BshFI GGCC 1 cut(s) 693
BshVI ATCGAT 1 cut(s) 300
BsiHKAI GWGCWC 2 cut(s) 142, 1030
BsiSI CCGG 4 cut(s) 287, 1286, 1449, 1593
BslFI GGGAC 2 cut(s) 772, 1137
BslI CCNNNNNNNGG 5 cut(s) 292, 424, 700, 821, 1074
BsmAI GTCTC 3 cut(s) 497, 1370, 1636
BsmFI GGGAC 2 cut(s) 772, 1137
BsmI GAATGC 1 cut(s) 1402
BsnI GGCC 1 cut(s) 693
Bso31I GGTCTC 2 cut(s) 497, 1636
Bsp1286I GDGCHC 2 cut(s) 142, 1030
Bsp1407I TGTACA 1 cut(s) 657
Bsp143I GATC 9 cut(s) 198, 301, 541, 624, 943, 1144, 1172, 1670, 1784
Bsp19I CCATGG 1 cut(s) 1068
BspACI CCGC 1 cut(s) 701
BspANI GGCC 1 cut(s) 693
BspCNI CTCAG 2 cut(s) 1293, 1540
BspDI ATCGAT 1 cut(s) 300
BspHI TCATGA 3 cut(s) 142, 225, 232
BspLI GGNNCC 3 cut(s) 1447, 1516, 1570
BspMI ACCTGC 3 cut(s) 175, 479, 508
BspPI GGATC 3 cut(s) 938, 1139, 1779
BspTNI GGTCTC 2 cut(s) 497, 1636
BsrDI GCAATG 2 cut(s) 95, 456
BsrFI RCCGGY 1 cut(s) 1448
BsrGI TGTACA 1 cut(s) 657
BsrI ACTGG 5 cut(s) 622, 1171, 1516, 1572, 1617
BssAI RCCGGY 1 cut(s) 1448
BssECI CCNNGG 2 cut(s) 967, 1068
BssMI GATC 9 cut(s) 198, 301, 541, 624, 943, 1144, 1172, 1670, 1784
BssNAI GTATAC 1 cut(s) 1483
BssT1I CCWWGG 1 cut(s) 1068
Bst1107I GTATAC 1 cut(s) 1483
Bst2UI CCWGG 2 cut(s) 1059, 1260
Bst4CI ACNGT 3 cut(s) 310, 755, 863
Bst6I CTCTTC 1 cut(s) 1767
BstAUI TGTACA 1 cut(s) 657
BstDEI CTNAG 6 cut(s) 318, 1037, 1190, 1301, 1358, 1548
BstDSI CCRYGG 1 cut(s) 1068
BstF5I GGATG 5 cut(s) 53, 126, 337, 564, 1079
BstKTI GATC 9 cut(s) 201, 304, 544, 627, 946, 1147, 1175, 1673, 1787
BstMAI GTCTC 3 cut(s) 497, 1370, 1636
BstMBI GATC 9 cut(s) 198, 301, 541, 624, 943, 1144, 1172, 1670, 1784
BstNI CCWGG 2 cut(s) 1059, 1260
BstNSI RCATGY 1 cut(s) 1013
BstPAI GACNNNNGTC 1 cut(s) 1470
BstSCI CCNGG 4 cut(s) 285, 1057, 1258, 1284
BstV1I GCAGC 3 cut(s) 818, 1399, 1568
BstV2I GAAGAC 2 cut(s) 36, 1225
BstX2I RGATCY 2 cut(s) 1670, 1784
BstXI CCANNNNNNTGG 1 cut(s) 533
BstYI RGATCY 2 cut(s) 1670, 1784
BstZ17I GTATAC 1 cut(s) 1483
Bsu15I ATCGAT 1 cut(s) 300
BsuRI GGCC 1 cut(s) 693
BsuTUI ATCGAT 1 cut(s) 300
BtgI CCRYGG 1 cut(s) 1068
BtsCI GGATG 5 cut(s) 53, 126, 337, 564, 1079
BtsIMutI CAGTG 3 cut(s) 270, 615, 766
BveI ACCTGC 3 cut(s) 175, 479, 508
CaiI CAGNNNCTG 1 cut(s) 1511
CciI TCATGA 3 cut(s) 142, 225, 232
Cfr10I RCCGGY 1 cut(s) 1448
Cfr13I GGNCC 2 cut(s) 692, 770
ClaI ATCGAT 1 cut(s) 300
CsiI ACCWGGT 1 cut(s) 1057
Csp6I GTAC 6 cut(s) 212, 647, 658, 1164, 1509, 1614
CviQI GTAC 6 cut(s) 212, 647, 658, 1164, 1509, 1614
DdeI CTNAG 6 cut(s) 318, 1037, 1190, 1301, 1358, 1548
DpnI GATC 9 cut(s) 200, 303, 543, 626, 945, 1146, 1174, 1672, 1786
DpnII GATC 9 cut(s) 198, 301, 541, 624, 943, 1144, 1172, 1670, 1784
DraIII CACNNNGTG 1 cut(s) 1330
Eam1104I CTCTTC 1 cut(s) 1767
EarI CTCTTC 1 cut(s) 1767
Ecl136II GAGCTC 2 cut(s) 140, 1028
Eco130I CCWWGG 1 cut(s) 1068
Eco24I GRGCYC 2 cut(s) 142, 1030
Eco31I GGTCTC 2 cut(s) 497, 1636
Eco32I GATATC 2 cut(s) 298, 469
Eco47I GGWCC 1 cut(s) 770
Eco53kI GAGCTC 2 cut(s) 140, 1028
Eco57I CTGAAG 1 cut(s) 350
EcoICRI GAGCTC 2 cut(s) 140, 1028
EcoRI GAATTC 2 cut(s) 389, 600
EcoRII CCWGG 2 cut(s) 1057, 1258
EcoRV GATATC 2 cut(s) 298, 469
EcoT14I CCWWGG 1 cut(s) 1068
EcoT22I ATGCAT 1 cut(s) 640
EcoT38I GRGCYC 2 cut(s) 142, 1030
ErhI CCWWGG 1 cut(s) 1068
FalI AAGNNNNNCTT 2 cut(s) 997, 1029
FaqI GGGAC 2 cut(s) 772, 1137
FauNDI CATATG 1 cut(s) 634
FblI GTMKAC 2 cut(s) 1482, 1604
Fnu4HI GCNGC 3 cut(s) 807, 1388, 1582
FokI GGATG 5 cut(s) 40, 133, 344, 551, 1086
FriOI GRGCYC 2 cut(s) 142, 1030
Fsp4HI GCNGC 3 cut(s) 807, 1388, 1582
FspBI CTAG 3 cut(s) 650, 1724, 1734
GluI GCNGC 3 cut(s) 807, 1388, 1582
GsuI CTGGAG 2 cut(s) 533, 798
HaeIII GGCC 1 cut(s) 693
HapII CCGG 4 cut(s) 287, 1286, 1449, 1593
HincII GTYRAC 3 cut(s) 523, 898, 1475
HindII GTYRAC 3 cut(s) 523, 898, 1475
HindIII AAGCTT 2 cut(s) 576, 1539
HinfI GANTC 9 cut(s) 92, 113, 229, 383, 595, 899, 1471, 1502, 1528
HpaII CCGG 4 cut(s) 287, 1286, 1449, 1593
HphI GGTGA 5 cut(s) 17, 1073, 1289, 1316, 1607
Hpy166II GTNNAC 5 cut(s) 523, 898, 1475, 1483, 1605
Hpy8I GTNNAC 5 cut(s) 523, 898, 1475, 1483, 1605
HpyAV CCTTC 1 cut(s) 800
HpyCH4III ACNGT 3 cut(s) 310, 755, 863
HpyF3I CTNAG 6 cut(s) 318, 1037, 1190, 1301, 1358, 1548
Kzo9I GATC 9 cut(s) 198, 301, 541, 624, 943, 1144, 1172, 1670, 1784
LmnI GCTCC 5 cut(s) 514, 886, 1025, 1568, 1684
Lsp1109I GCAGC 3 cut(s) 818, 1399, 1568
LweI GCATC 3 cut(s) 97, 570, 818
MabI ACCWGGT 1 cut(s) 1057
MaeI CTAG 3 cut(s) 650, 1724, 1734
MaeIII GTNAC 8 cut(s) 63, 979, 1061, 1097, 1150, 1322, 1330, 1619
MalI GATC 9 cut(s) 200, 303, 543, 626, 945, 1146, 1174, 1672, 1786
MboI GATC 9 cut(s) 198, 301, 541, 624, 943, 1144, 1172, 1670, 1784
MboII GAAGA 8 cut(s) 30, 36, 39, 386, 1182, 1230, 1643, 1784
MfeI CAATTG 2 cut(s) 350, 1696
MflI RGATCY 2 cut(s) 1670, 1784
MhlI GDGCHC 2 cut(s) 142, 1030
MluCI AATT 9 cut(s) 9, 240, 350, 389, 600, 1178, 1678, 1696, 1703
MlyI GAGTC 4 cut(s) 238, 604, 893, 1480
MmeI TCCRAC 3 cut(s) 187, 908, 1021
Mph1103I ATGCAT 1 cut(s) 640
MroXI GAANNNNTTC 1 cut(s) 1349
MseI TTAA 2 cut(s) 477, 854
MslI CAYNNNNRTG 1 cut(s) 1302
MspI CCGG 4 cut(s) 287, 1286, 1449, 1593
MspR9I CCNGG 4 cut(s) 287, 1059, 1260, 1286
MunI CAATTG 2 cut(s) 350, 1696
Mva1269I GAATGC 1 cut(s) 1402
MvaI CCWGG 2 cut(s) 1059, 1260
NciI CCSGG 2 cut(s) 287, 1286
NcoI CCATGG 1 cut(s) 1068
NdeI CATATG 1 cut(s) 634
NdeII GATC 9 cut(s) 198, 301, 541, 624, 943, 1144, 1172, 1670, 1784
NlaIV GGNNCC 3 cut(s) 1447, 1516, 1570
NmeAIII GCCGAG 1 cut(s) 948
NmuCI GTSAC 4 cut(s) 63, 1061, 1322, 1330
NsiI ATGCAT 1 cut(s) 640
NspI RCATGY 1 cut(s) 1013
OliI CACNNNNGTG 1 cut(s) 1302
PagI TCATGA 3 cut(s) 142, 225, 232
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PctI GAATGC 1 cut(s) 1402
PdmI GAANNNNTTC 1 cut(s) 1349
PfeI GAWTC 5 cut(s) 92, 113, 383, 1502, 1528
PflMI CCANNNNNTGG 2 cut(s) 821, 1074
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 3 cut(s) 808, 1389, 1583
PleI GAGTC 4 cut(s) 237, 603, 893, 1479
PpsI GAGTC 4 cut(s) 237, 603, 893, 1479
PshAI GACNNNNGTC 1 cut(s) 1470
Psp124BI GAGCTC 2 cut(s) 142, 1030
Psp6I CCWGG 2 cut(s) 1057, 1258
PspGI CCWGG 2 cut(s) 1057, 1258
PspN4I GGNNCC 3 cut(s) 1447, 1516, 1570
PspPI GGNCC 2 cut(s) 692, 770
PstNI CAGNNNCTG 1 cut(s) 1511
PsuI RGATCY 2 cut(s) 1670, 1784
RsaI GTAC 6 cut(s) 213, 648, 659, 1165, 1510, 1615
RsaNI GTAC 6 cut(s) 212, 647, 658, 1164, 1509, 1614
RseI CAYNNNNRTG 1 cut(s) 1302
SacI GAGCTC 2 cut(s) 142, 1030
SaqAI TTAA 2 cut(s) 477, 854
SatI GCNGC 3 cut(s) 807, 1388, 1582
Sau3AI GATC 9 cut(s) 198, 301, 541, 624, 943, 1144, 1172, 1670, 1784
Sau96I GGNCC 2 cut(s) 692, 770
ScaI AGTACT 1 cut(s) 648
SchI GAGTC 4 cut(s) 238, 604, 893, 1480
ScrFI CCNGG 4 cut(s) 287, 1059, 1260, 1286
SduI GDGCHC 2 cut(s) 142, 1030
SexAI ACCWGGT 1 cut(s) 1057
SfaNI GCATC 3 cut(s) 97, 570, 818
SinI GGWCC 1 cut(s) 770
SmiMI CAYNNNNRTG 1 cut(s) 1302
SmlI CTYRAG 1 cut(s) 911
SmoI CTYRAG 1 cut(s) 911
SpeI ACTAGT 1 cut(s) 649
Sse9I AATT 9 cut(s) 9, 240, 350, 389, 600, 1178, 1678, 1696, 1703
SsiI CCGC 1 cut(s) 701
SspMI CTAG 3 cut(s) 650, 1724, 1734
SstI GAGCTC 2 cut(s) 142, 1030
StyD4I CCNGG 4 cut(s) 285, 1057, 1258, 1284
StyI CCWWGG 1 cut(s) 1068
TaaI ACNGT 3 cut(s) 310, 755, 863
TaqI TCGA 4 cut(s) 300, 598, 1030, 1203
TasI AATT 9 cut(s) 9, 240, 350, 389, 600, 1178, 1678, 1696, 1703
TatI WGTACW 2 cut(s) 646, 657
TfiI GAWTC 5 cut(s) 92, 113, 383, 1502, 1528
Tru1I TTAA 2 cut(s) 477, 854
Tru9I TTAA 2 cut(s) 477, 854
TscAI CASTG 3 cut(s) 270, 622, 766
TseFI GTSAC 4 cut(s) 63, 1061, 1322, 1330
TseI GCWGC 3 cut(s) 806, 1387, 1581
Tsp45I GTSAC 4 cut(s) 63, 1061, 1322, 1330
TspDTI ATGAA 8 cut(s) 21, 39, 84, 243, 387, 665, 669, 723
TspRI CASTG 3 cut(s) 270, 622, 766
Van91I CCANNNNNTGG 2 cut(s) 821, 1074
VpaK11BI GGWCC 1 cut(s) 770
XapI RAATTY 4 cut(s) 9, 389, 600, 1678
XceI RCATGY 1 cut(s) 1013
XmiI GTMKAC 2 cut(s) 1482, 1604
XmnI GAANNNNTTC 1 cut(s) 1349
XspI CTAG 3 cut(s) 650, 1724, 1734
ZrmI AGTACT 1 cut(s) 648
Zsp2I ATGCAT 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.