RchiOBHm_Chr5g0072681

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001135
Physical Location & Seq
Reverse (-)
78760896 .. 78765507
4612 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ34762

Sequence Viewer

Length: 1947 bp
ATGACCCAGGTCTGGTCGCCGGAGCCGAACCGCGCCGTGTTGCCGGAATTTCGTTCCTGCCCGTCCTCGCTGCCATTGCCGGCGACCAGTCCCGATCCGTCGGCAATTGGGCCGGAGTCTTGGGCCGGAGCCGAGCGGGCCACCCGGGATATACTGTCCAAGATTCAGCCCACGGCGGCGACTCACCGGAGAAGGAAGGATATCATTGTATATGTGCAGAGCCTCATCACTTGTAACGTGGGCTGTCAGGTTTTTCCTTATGGGTCAGTTCCATTAAAGACATATCTTCCCGATGGAGATATAGACTTGACGGTTTTCACTGGCAGTGCGCACCTTGAGGATGTCCTTGTAACTCATGTTCATGCTGTTCTGAAGGGGGAAGAGAATAATGAAGCTGCTCAGTATCAAGTGAAGGATGTCCATCGCATTGATGCTGAGGTTAAACTTGTAAAATGCATTGTTCAAAATATGATTGTAGATATGTCCTTCAATCAGTTAGGAGGACTTTCTACACTACGCTTTCTTGAACAGGTTGATCACTATATTGGCAAAGATCATATTTTCAAACGCAGCATTATTCTGATAAAAGCCTGGTGCTACTATGAGAGTCGTATTCTTGGTGCTCATCATGGTTTAATTTCAACATATGCTTTGGAAACATTAGTCCTATATATATTCCATCTTTTTCATTCTTCTCTAGATGGCCCTTTAACGGTCCTCTATAGATTTTTGGATTACTTCAGCAAATTTGATTGGGAGAAATATTGTGTTAGTTTAAATGGACCAGTTTGCAAATCCTCCCTACCTGATATTGTGGCTGAAGTACCAGATAATGGAGGGGATGATGTACTGCTAAGTGAAGAGTTTATTAGAAACTGTGTAGACATGTTCTCTGTTCCATCAAAGGGGTGTGAGACAAACTTAAGAGTGTTTTCCTTGAAGCATCTTAATATAATCGATCCACTAAAAGAGAACAACAACCTTGGCCGTAGTGTCAATAGAGGTAGCTTCTATCGAATATGTAGTGCTTTTAAATATGGAGCTCGCAAGCTAGGCTGGATTCTCTTGTTACCAGCAGAGAGAATATCAGATGAACTTAACAAGTTTTTCGCCAACACTTTGGATAGGCATGAAAGTAAATGCCAGACTACTGTGCAAAATAATGCCTTGTTGGAGCTTGGTGAAGATTTGGACAGTCATTGTTTGAGCCTGCAATATAGTCTGATTGGCCAGAGGTGTGCTACATCACCTCCTTTATTGCCCAGTCCTCCCTTGTCACCTCAACCATGGAAAGAGAATTCATGGGATGCTACCTGCGAAGCCCTACAGTCCCAGCACAATATTAATTCTCAAACTAATGCAAATGGTATTCCTTGGGGAACACATGCGTATCACACAAATGGTGCCAGTTTTTTTGTTCCTCCATTCAGAGAAGAGAAGAACAAACCACGAGGAACAGGGACGTACATCCCCAATGCGTCTTTTCAGAATTATCGTCCCTACAAGGATAGACACTTGCCTGGAAGGGGAAGGAAAGAGGCAGCTGGAACTTATGCTCCATTTCAGAGACACACTTTCAATTATGGCTTTCCTGCAGCATCTCAAGAGTCAATCGCACCTGGAGAATGCAACCATGAGCTCTCAGAAGCTGAGTACCCAGTTTTAAGTAATGCAAACTCTGCAACATCAGATTACCATCAACCTCAGCTTTCAATTTGGGAGCCTTTCCATGAAAATGGTTTCTCCAAATCCCCCGACAAACCTGAGTCTAGATCCTCCTCCCCTCAATCATGGGAAGAAGTACCCATGCCACAACTGGACAGTTTACGGTTATCAGGAGTCGATGAAGAGAGGATTGACGAGCAGGCGTTCCATCTGAAGGATGAAGCTGATTTCCCCCCGCTCACCCCTCGACTGCCTTTGAAGGACGGAATATGCATTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAP_assoc

Protein Analysis

648

Amino Acids

72.82

Weight (kDa)

5.81

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 48 - 175 6.2e-08 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
PAP-OAS1 PF26180 182 - 374 6.7e-100 PAP/OAS1 substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 330
Acc36I ACCTGC 1 cut(s) 1322
AccB1I GGYRCC 1 cut(s) 1403
AccB7I CCANNNNNTGG 1 cut(s) 833
AccBSI CCGCTC 2 cut(s) 136, 1903
AccI GTMKAC 1 cut(s) 882
AccII CGCG 1 cut(s) 33
AciI CCGC 4 cut(s) 31, 136, 176, 1901
AclWI GGATC 3 cut(s) 89, 953, 1767
AcoI YGGCCR 2 cut(s) 985, 1228
AcsI RAATTY 3 cut(s) 47, 746, 1297
AcuI CTGAAG 4 cut(s) 392, 724, 840, 1898
AfaI GTAC 5 cut(s) 825, 849, 1466, 1655, 1803
AfiI CCNNNNNNNGG 6 cut(s) 12, 712, 833, 905, 1526, 1879
AflII CTTAAG 1 cut(s) 922
AflIII ACRYGT 1 cut(s) 885
AjnI CCWGG 4 cut(s) 6, 590, 1519, 1618
AloI GAACNNNNNNTCC 2 cut(s) 1540, 1572
Alw21I GWGCWC 3 cut(s) 625, 1045, 1641
Alw26I GTCTC 2 cut(s) 908, 1561
AlwI GGATC 3 cut(s) 89, 953, 1767
AlwNI CAGNNNCTG 1 cut(s) 1649
Ama87I CYCGRG 1 cut(s) 144
AoxI GGCC 6 cut(s) 110, 123, 138, 703, 985, 1228
ApeKI GCWGC 5 cut(s) 70, 395, 570, 1541, 1595
ApoI RAATTY 3 cut(s) 47, 746, 1297
AseI ATTAAT 1 cut(s) 1344
AspLEI GCGC 2 cut(s) 35, 331
AspS9I GGNCC 6 cut(s) 110, 123, 138, 704, 715, 782
AsuC2I CCSGG 2 cut(s) 145, 146
AsuHPI GGTGA 5 cut(s) 176, 1193, 1239, 1269, 1897
AvaI CYCGRG 1 cut(s) 144
AvaII GGWCC 2 cut(s) 715, 782
BaeI ACNNNNGTAYC 2 cut(s) 1373, 1406
BalI TGGCCA 1 cut(s) 1230
BanI GGYRCC 1 cut(s) 1403
BanII GRGCYC 2 cut(s) 1045, 1641
BauI CACGAG 1 cut(s) 1449
Bbv12I GWGCWC 3 cut(s) 625, 1045, 1641
BbvCI CCTCAGC 2 cut(s) 435, 1704
BbvI GCAGC 5 cut(s) 57, 382, 582, 1553, 1607
BccI CCATC 7 cut(s) 287, 429, 687, 695, 907, 1704, 1881
BceAI ACGGC 3 cut(s) 20, 189, 972
BciT130I CCWGG 4 cut(s) 8, 592, 1521, 1620
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 2 cut(s) 145, 146
BcoDI GTCTC 2 cut(s) 908, 1561
BfaI CTAG 3 cut(s) 698, 1052, 1770
BfmI CTRYAG 3 cut(s) 721, 1325, 1593
BfrI CTTAAG 1 cut(s) 922
BfuAI ACCTGC 1 cut(s) 1322
BglI GCCNNNNNGGC 1 cut(s) 137
BisI GCNGC 6 cut(s) 71, 177, 396, 571, 1542, 1596
BlsI GCNGC 6 cut(s) 72, 178, 397, 572, 1543, 1597
Bme1390I CCNGG 6 cut(s) 8, 145, 146, 592, 1521, 1620
Bme18I GGWCC 2 cut(s) 715, 782
BmeT110I CYCGRG 1 cut(s) 144
BmgT120I GGNCC 6 cut(s) 110, 123, 138, 704, 715, 782
BmiI GGNNCC 4 cut(s) 24, 130, 1405, 1722
BmrFI CCNGG 6 cut(s) 8, 145, 146, 592, 1521, 1620
BmrI ACTGGG 2 cut(s) 1257, 1652
BmsI GCATC 4 cut(s) 421, 952, 1297, 1607
BmuI ACTGGG 2 cut(s) 1257, 1652
BoxI GACNNNNGTC 1 cut(s) 8
BpmI CTGGAG 1 cut(s) 1641
Bpu10I CCTNAGC 2 cut(s) 435, 1704
BpuEI CTTGAG 2 cut(s) 356, 1587
BpuMI CCSGG 2 cut(s) 145, 146
Bsa29I ATCGAT 1 cut(s) 957
BsaBI GATNNNNATC 2 cut(s) 420, 1695
BsaJI CCNNGG 6 cut(s) 6, 144, 171, 982, 1286, 1373
BsaWI WCCGGW 1 cut(s) 186
BsaXI ACNNNNNCTCC 4 cut(s) 1234, 1264, 1540, 1570
Bsc4I CCNNNNNNNGG 6 cut(s) 12, 712, 833, 905, 1526, 1879
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 7 cut(s) 87, 325, 785, 1263, 1407, 1658, 1821
Bse3DI GCAATG 1 cut(s) 74
Bse8I GATNNNNATC 2 cut(s) 420, 1695
BseBI CCWGG 4 cut(s) 8, 592, 1521, 1620
BseCI ATCGAT 1 cut(s) 957
BseDI CCNNGG 6 cut(s) 6, 144, 171, 982, 1286, 1373
BseGI GGATG 6 cut(s) 346, 421, 847, 1312, 1467, 1888
BseJI GATNNNNATC 2 cut(s) 420, 1695
BseLI CCNNNNNNNGG 6 cut(s) 12, 712, 833, 905, 1526, 1879
BseMI GCAATG 1 cut(s) 74
BseMII CTCAG 6 cut(s) 413, 426, 1641, 1656, 1718, 1755
BseNI ACTGG 7 cut(s) 87, 325, 785, 1263, 1407, 1658, 1821
BseRI GAGGAG 1 cut(s) 1768
BseXI GCAGC 5 cut(s) 57, 382, 582, 1553, 1607
BseYI CCCAGC 1 cut(s) 1332
BsgI GTGCAG 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 6 cut(s) 112, 125, 140, 705, 987, 1230
BshNI GGYRCC 1 cut(s) 1403
BshVI ATCGAT 1 cut(s) 957
BsiHKAI GWGCWC 3 cut(s) 625, 1045, 1641
BsiHKCI CYCGRG 1 cut(s) 144
BsiSI CCGG 7 cut(s) 20, 44, 80, 113, 126, 145, 187
BslFI GGGAC 4 cut(s) 75, 1315, 1474, 1482
BslI CCNNNNNNNGG 6 cut(s) 12, 712, 833, 905, 1526, 1879
BsmAI GTCTC 2 cut(s) 908, 1561
BsmFI GGGAC 4 cut(s) 75, 1315, 1474, 1482
BsmI GAATGC 2 cut(s) 1631, 1938
BsnI GGCC 6 cut(s) 112, 125, 140, 705, 987, 1230
BsoBI CYCGRG 1 cut(s) 144
Bsp1286I GDGCHC 3 cut(s) 625, 1045, 1641
Bsp143I GATC 5 cut(s) 94, 535, 553, 958, 1772
Bsp19I CCATGG 1 cut(s) 1286
BspACI CCGC 4 cut(s) 31, 136, 176, 1901
BspANI GGCC 6 cut(s) 112, 125, 140, 705, 987, 1230
BspCNI CTCAG 6 cut(s) 412, 427, 1642, 1655, 1717, 1756
BspDI ATCGAT 1 cut(s) 957
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 4 cut(s) 24, 130, 1405, 1722
BspMAI CTGCAG 1 cut(s) 1597
BspMI ACCTGC 1 cut(s) 1322
BspPI GGATC 3 cut(s) 89, 953, 1767
BspT107I GGYRCC 1 cut(s) 1403
BspTI CTTAAG 1 cut(s) 922
BsrBI CCGCTC 2 cut(s) 136, 1903
BsrDI GCAATG 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 7 cut(s) 87, 325, 785, 1263, 1407, 1658, 1821
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 6 cut(s) 6, 144, 171, 982, 1286, 1373
BssMI GATC 5 cut(s) 94, 535, 553, 958, 1772
BssSI CACGAG 1 cut(s) 1449
BssT1I CCWWGG 3 cut(s) 982, 1286, 1373
Bst2BI CACGAG 1 cut(s) 1449
Bst2UI CCWGG 4 cut(s) 8, 592, 1521, 1620
Bst4CI ACNGT 9 cut(s) 156, 313, 715, 878, 1153, 1196, 1329, 1823, 1830
Bst6I CTCTTC 4 cut(s) 375, 855, 1428, 1842
BstAFI CTTAAG 1 cut(s) 922
BstAPI GCANNNNNTGC 1 cut(s) 1679
BstC8I GCNNGC 6 cut(s) 81, 138, 1045, 1049, 1211, 1866
BstDEI CTNAG 7 cut(s) 399, 435, 854, 1642, 1650, 1704, 1764
BstDSI CCRYGG 2 cut(s) 171, 1286
BstF5I GGATG 6 cut(s) 346, 421, 847, 1312, 1467, 1888
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 2 cut(s) 35, 331
BstKTI GATC 5 cut(s) 97, 538, 556, 961, 1775
BstMAI GTCTC 2 cut(s) 908, 1561
BstMBI GATC 5 cut(s) 94, 535, 553, 958, 1772
BstMWI GCNNNNNNNGC 4 cut(s) 76, 137, 1053, 1679
BstNI CCWGG 4 cut(s) 8, 592, 1521, 1620
BstNSI RCATGY 2 cut(s) 889, 1388
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 6 cut(s) 6, 143, 144, 590, 1519, 1618
BstSFI CTRYAG 3 cut(s) 721, 1325, 1593
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 5 cut(s) 57, 382, 582, 1553, 1607
BstX2I RGATCY 1 cut(s) 1772
BstXI CCANNNNNNTGG 2 cut(s) 1120, 1736
BstYI RGATCY 1 cut(s) 1772
Bsu15I ATCGAT 1 cut(s) 957
BsuRI GGCC 6 cut(s) 112, 125, 140, 705, 987, 1230
BsuTUI ATCGAT 1 cut(s) 957
BtgI CCRYGG 2 cut(s) 171, 1286
BtgZI GCGATG 1 cut(s) 407
BtsCI GGATG 6 cut(s) 346, 421, 847, 1312, 1467, 1888
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 2 cut(s) 318, 331
BveI ACCTGC 1 cut(s) 1322
Cac8I GCNNGC 6 cut(s) 81, 138, 1045, 1049, 1211, 1866
CaiI CAGNNNCTG 1 cut(s) 1649
CfoI GCGC 2 cut(s) 35, 331
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 6 cut(s) 110, 123, 138, 704, 715, 782
Cfr9I CCCGGG 1 cut(s) 144
ClaI ATCGAT 1 cut(s) 957
CseI GACGC 1 cut(s) 1467
Csp6I GTAC 5 cut(s) 824, 848, 1465, 1654, 1802
CviQI GTAC 5 cut(s) 824, 848, 1465, 1654, 1802
DdeI CTNAG 7 cut(s) 399, 435, 854, 1642, 1650, 1704, 1764
DpnI GATC 5 cut(s) 96, 537, 555, 960, 1774
DpnII GATC 5 cut(s) 94, 535, 553, 958, 1772
DraI TTTAAA 2 cut(s) 777, 1033
EaeI YGGCCR 2 cut(s) 985, 1228
Eam1104I CTCTTC 4 cut(s) 375, 855, 1428, 1842
EarI CTCTTC 4 cut(s) 375, 855, 1428, 1842
Ecl136II GAGCTC 2 cut(s) 1043, 1639
Eco130I CCWWGG 3 cut(s) 982, 1286, 1373
Eco24I GRGCYC 2 cut(s) 1045, 1641
Eco32I GATATC 1 cut(s) 202
Eco47I GGWCC 2 cut(s) 715, 782
Eco53kI GAGCTC 2 cut(s) 1043, 1639
Eco57I CTGAAG 4 cut(s) 392, 724, 840, 1898
Eco88I CYCGRG 1 cut(s) 144
EcoICRI GAGCTC 2 cut(s) 1043, 1639
EcoRI GAATTC 1 cut(s) 1297
EcoRII CCWGG 4 cut(s) 6, 590, 1519, 1618
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 3 cut(s) 982, 1286, 1373
EcoT22I ATGCAT 2 cut(s) 458, 1940
EcoT38I GRGCYC 2 cut(s) 1045, 1641
ErhI CCWWGG 3 cut(s) 982, 1286, 1373
FaqI GGGAC 4 cut(s) 75, 1315, 1474, 1482
FauI CCCGC 2 cut(s) 129, 1908
FauNDI CATATG 1 cut(s) 646
FbaI TGATCA 1 cut(s) 535
FblI GTMKAC 1 cut(s) 882
Fnu4HI GCNGC 6 cut(s) 71, 177, 396, 571, 1542, 1596
FokI GGATG 6 cut(s) 353, 428, 854, 1319, 1454, 1895
FriOI GRGCYC 2 cut(s) 1045, 1641
Fsp4HI GCNGC 6 cut(s) 71, 177, 396, 571, 1542, 1596
FspAI RTGCGCAY 1 cut(s) 330
FspBI CTAG 3 cut(s) 698, 1052, 1770
FspI TGCGCA 1 cut(s) 330
GlaI GCGC 2 cut(s) 34, 330
GluI GCNGC 6 cut(s) 71, 177, 396, 571, 1542, 1596
GsaI CCCAGC 1 cut(s) 1336
GsuI CTGGAG 1 cut(s) 1641
HaeIII GGCC 6 cut(s) 112, 125, 140, 705, 987, 1230
HapII CCGG 7 cut(s) 20, 44, 80, 113, 126, 145, 187
HgaI GACGC 1 cut(s) 1467
HhaI GCGC 2 cut(s) 35, 331
Hin6I GCGC 2 cut(s) 33, 329
HinP1I GCGC 2 cut(s) 33, 329
HinfI GANTC 8 cut(s) 116, 163, 181, 607, 1060, 1607, 1766, 1839
HpaII CCGG 7 cut(s) 20, 44, 80, 113, 126, 145, 187
HphI GGTGA 5 cut(s) 176, 1193, 1239, 1269, 1897
Hpy166II GTNNAC 2 cut(s) 883, 1826
Hpy188III TCNNGA 7 cut(s) 92, 290, 524, 698, 1604, 1770, 1836
Hpy8I GTNNAC 2 cut(s) 883, 1826
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 9 cut(s) 186, 190, 367, 406, 496, 1518, 1524, 1873, 1918
HpyCH4III ACNGT 9 cut(s) 156, 313, 715, 878, 1153, 1196, 1329, 1823, 1830
HpyCH4IV ACGT 2 cut(s) 237, 1463
HpyF10VI GCNNNNNNNGC 4 cut(s) 76, 137, 1053, 1679
HpyF3I CTNAG 7 cut(s) 399, 435, 854, 1642, 1650, 1704, 1764
HpySE526I ACGT 2 cut(s) 237, 1463
HspAI GCGC 2 cut(s) 33, 329
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 5 cut(s) 94, 535, 553, 958, 1772
LmnI GCTCC 6 cut(s) 22, 128, 1040, 1174, 1561, 1720
Lsp1109I GCAGC 5 cut(s) 57, 382, 582, 1553, 1607
LweI GCATC 4 cut(s) 421, 952, 1297, 1607
MaeI CTAG 3 cut(s) 698, 1052, 1770
MaeII ACGT 2 cut(s) 237, 1463
MaeIII GTNAC 4 cut(s) 233, 349, 1068, 1275
MalI GATC 5 cut(s) 96, 537, 555, 960, 1774
MbiI CCGCTC 2 cut(s) 136, 1903
MboI GATC 5 cut(s) 94, 535, 553, 958, 1772
MboII GAAGA 9 cut(s) 278, 392, 684, 872, 1196, 1445, 1450, 1808, 1859
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 1 cut(s) 1772
MhlI GDGCHC 3 cut(s) 625, 1045, 1641
MlsI TGGCCA 1 cut(s) 1230
MluCI AATT 9 cut(s) 47, 105, 636, 746, 1297, 1345, 1489, 1579, 1713
MluNI TGGCCA 1 cut(s) 1230
MlyI GAGTC 6 cut(s) 125, 175, 616, 1616, 1775, 1848
MmeI TCCRAC 1 cut(s) 1152
Mox20I TGGCCA 1 cut(s) 1230
Mph1103I ATGCAT 2 cut(s) 458, 1940
MroNI GCCGGC 1 cut(s) 79
MscI TGGCCA 1 cut(s) 1230
MslI CAYNNNNRTG 3 cut(s) 360, 1398, 1734
Msp20I TGGCCA 1 cut(s) 1230
MspA1I CMGCKG 1 cut(s) 1544
MspCI CTTAAG 1 cut(s) 922
MspI CCGG 7 cut(s) 20, 44, 80, 113, 126, 145, 187
MspR9I CCNGG 6 cut(s) 8, 145, 146, 592, 1521, 1620
MunI CAATTG 1 cut(s) 105
Mva1269I GAATGC 2 cut(s) 1631, 1938
MvaI CCWGG 4 cut(s) 8, 592, 1521, 1620
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 4 cut(s) 76, 137, 1053, 1679
NaeI GCCGGC 1 cut(s) 81
NciI CCSGG 2 cut(s) 145, 146
NcoI CCATGG 1 cut(s) 1286
NdeI CATATG 1 cut(s) 646
NdeII GATC 5 cut(s) 94, 535, 553, 958, 1772
NgoMIV GCCGGC 1 cut(s) 79
NlaIV GGNNCC 4 cut(s) 24, 130, 1405, 1722
NmeAIII GCCGAG 1 cut(s) 157
NmuCI GTSAC 1 cut(s) 1275
NsbI TGCGCA 1 cut(s) 330
NsiI ATGCAT 2 cut(s) 458, 1940
NspI RCATGY 2 cut(s) 889, 1388
PciI ACATGT 1 cut(s) 885
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 2 cut(s) 1631, 1938
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 163, 1060
PflMI CCANNNNNTGG 1 cut(s) 833
PkrI GCNGC 6 cut(s) 72, 178, 397, 572, 1543, 1597
PleI GAGTC 6 cut(s) 124, 175, 615, 1615, 1774, 1847
PpsI GAGTC 6 cut(s) 124, 175, 615, 1615, 1774, 1847
PscI ACATGT 1 cut(s) 885
PshAI GACNNNNGTC 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 1344
Psp124BI GAGCTC 2 cut(s) 1045, 1641
Psp6I CCWGG 4 cut(s) 6, 590, 1519, 1618
PspFI CCCAGC 1 cut(s) 1332
PspGI CCWGG 4 cut(s) 6, 590, 1519, 1618
PspN4I GGNNCC 4 cut(s) 24, 130, 1405, 1722
PspPI GGNCC 6 cut(s) 110, 123, 138, 704, 715, 782
PsrI GAACNNNNNNTAC 2 cut(s) 342, 374
PstI CTGCAG 1 cut(s) 1597
PstNI CAGNNNCTG 1 cut(s) 1649
PsuI RGATCY 1 cut(s) 1772
PvuII CAGCTG 1 cut(s) 1544
RsaI GTAC 5 cut(s) 825, 849, 1466, 1655, 1803
RsaNI GTAC 5 cut(s) 824, 848, 1465, 1654, 1802
RseI CAYNNNNRTG 3 cut(s) 360, 1398, 1734
SacI GAGCTC 2 cut(s) 1045, 1641
SatI GCNGC 6 cut(s) 71, 177, 396, 571, 1542, 1596
Sau3AI GATC 5 cut(s) 94, 535, 553, 958, 1772
Sau96I GGNCC 6 cut(s) 110, 123, 138, 704, 715, 782
SchI GAGTC 6 cut(s) 125, 175, 616, 1616, 1775, 1848
ScrFI CCNGG 6 cut(s) 8, 145, 146, 592, 1521, 1620
SduI GDGCHC 3 cut(s) 625, 1045, 1641
SfaNI GCATC 4 cut(s) 421, 952, 1297, 1607
SfcI CTRYAG 3 cut(s) 721, 1325, 1593
SinI GGWCC 2 cut(s) 715, 782
SmaI CCCGGG 1 cut(s) 146
SmiMI CAYNNNNRTG 3 cut(s) 360, 1398, 1734
SmlI CTYRAG 3 cut(s) 335, 922, 1602
SmoI CTYRAG 3 cut(s) 335, 922, 1602
Sse9I AATT 9 cut(s) 47, 105, 636, 746, 1297, 1345, 1489, 1579, 1713
SsiI CCGC 4 cut(s) 31, 136, 176, 1901
SspI AATATT 2 cut(s) 764, 1342
SspMI CTAG 3 cut(s) 698, 1052, 1770
SstI GAGCTC 2 cut(s) 1045, 1641
StyD4I CCNGG 6 cut(s) 6, 143, 144, 590, 1519, 1618
StyI CCWWGG 3 cut(s) 982, 1286, 1373
TaaI ACNGT 9 cut(s) 156, 313, 715, 878, 1153, 1196, 1329, 1823, 1830
TaiI ACGT 2 cut(s) 240, 1466
TaqI TCGA 4 cut(s) 957, 1015, 1842, 1912
TasI AATT 9 cut(s) 47, 105, 636, 746, 1297, 1345, 1489, 1579, 1713
TatI WGTACW 1 cut(s) 847
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 2 cut(s) 163, 1060
TscAI CASTG 2 cut(s) 325, 331
TseFI GTSAC 1 cut(s) 1275
TseI GCWGC 5 cut(s) 70, 395, 570, 1541, 1595
Tsp45I GTSAC 1 cut(s) 1275
TspDTI ATGAA 9 cut(s) 350, 405, 677, 1107, 1146, 1290, 1746, 1860, 1899
TspGWI ACGGA 2 cut(s) 87, 1944
TspMI CCCGGG 1 cut(s) 144
TspRI CASTG 2 cut(s) 325, 331
Van91I CCANNNNNTGG 1 cut(s) 833
Vha464I CTTAAG 1 cut(s) 922
VpaK11BI GGWCC 2 cut(s) 715, 782
VspI ATTAAT 1 cut(s) 1344
XapI RAATTY 3 cut(s) 47, 746, 1297
XbaI TCTAGA 2 cut(s) 697, 1769
XceI RCATGY 2 cut(s) 889, 1388
XcmI CCANNNNNNNNNTGG 1 cut(s) 1813
XmaI CCCGGG 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 882
XspI CTAG 3 cut(s) 698, 1052, 1770
Zsp2I ATGCAT 2 cut(s) 458, 1940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.