Rorug05G0419200

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001135
Physical Location & Seq
Forward (+)
58324127 .. 58325321
1195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0419200.1

Sequence Viewer

Length: 570 bp
ATGCCGCTGCCTGACCCCGAACCGGGTCAGTATATCAGAGAGCTAGGGGACTTTAGAGGCTGCCTGTGTGCATTTGATTATAAGAATCAACATATTGGCTTTATTTATTTGTGGGTCATGAAAGAATATAACGTGGGTGAGTCCTGGACTAAGCTCTTTACATTAAAGATTTCCGATCAGCCTATGCCTGTACTTTTTTTAAGGCCGATCATGGTTATGGAAACTAGTACAATTCTGGAGATAGGGATTTCTAATGAGGATAGGCATTTGGATACTGAGTTCAAGTTGATAAAGAGTTGTCACAAGGAAGAGAAGTTTGAAACACTAAGGGTCAGCAGGAAGATGTATGGCAAAATTATTGGATATGAAGAGAGTCTACTTTGGCTTGGAGACCATAACAACAGGACCTGCACTATGGAACTAGCCAAGAAGGCTGGGAATGCTTCAGAAGCTGGGGGGCAGAATAATGGTGAACAAGGCAGAATGAATGTTAATGTGGATAACCAAGGGTCAAATCCTGTCAATGTGTGTGTTCAAAACCATGGCAGCAGCTCTATTGTTTTTCCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAP_assoc

Protein Analysis

189

Amino Acids

21.51

Weight (kDa)

5.75

Isoelectric Point (pI)

43.03

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 81
Acc36I ACCTGC 1 cut(s) 416
AccI GTMKAC 1 cut(s) 376
AciI CCGC 1 cut(s) 5
AcuI CTGAAG 1 cut(s) 429
AfaI GTAC 2 cut(s) 192, 229
AfiI CCNNNNNNNGG 2 cut(s) 22, 23
AgsI TTSAA 3 cut(s) 283, 320, 536
AhlI ACTAGT 1 cut(s) 224
AjnI CCWGG 1 cut(s) 143
AloI GAACNNNNNNTCC 2 cut(s) 263, 295
AluBI AGCT 4 cut(s) 43, 154, 452, 552
AluI AGCT 4 cut(s) 43, 154, 452, 552
Alw26I GTCTC 1 cut(s) 384
AlwNI CAGNNNCTG 2 cut(s) 408, 452
AoxI GGCC 1 cut(s) 203
ApeKI GCWGC 4 cut(s) 7, 60, 546, 549
AspS9I GGNCC 1 cut(s) 405
AsuC2I CCSGG 1 cut(s) 24
AsuHPI GGTGA 2 cut(s) 149, 482
AvaII GGWCC 1 cut(s) 405
BarI GAAGNNNNNNTAC 2 cut(s) 360, 392
BbvI GCAGC 3 cut(s) 47, 558, 561
BciT130I CCWGG 1 cut(s) 145
BciVI GTATCC 1 cut(s) 265
BcnI CCSGG 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 384
BcuI ACTAGT 1 cut(s) 224
BfaI CTAG 3 cut(s) 44, 225, 422
BfuAI ACCTGC 1 cut(s) 416
BfuI GTATCC 1 cut(s) 265
BglI GCCNNNNNGGC 1 cut(s) 431
BisI GCNGC 5 cut(s) 5, 8, 61, 547, 550
BlsI GCNGC 5 cut(s) 6, 9, 62, 548, 551
Bme1390I CCNGG 2 cut(s) 24, 145
Bme18I GGWCC 1 cut(s) 405
BmgT120I GGNCC 1 cut(s) 405
BmrFI CCNGG 2 cut(s) 24, 145
BpmI CTGGAG 1 cut(s) 257
BpuMI CCSGG 1 cut(s) 24
BsaI GGTCTC 1 cut(s) 384
BsaJI CCNNGG 2 cut(s) 505, 541
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 23
BseBI CCWGG 1 cut(s) 145
BseDI CCNNGG 2 cut(s) 505, 541
BseLI CCNNNNNNNGG 2 cut(s) 22, 23
BseMII CTCAG 1 cut(s) 267
BseXI GCAGC 3 cut(s) 47, 558, 561
BseYI CCCAGC 2 cut(s) 434, 452
BsgI GTGCAG 1 cut(s) 394
BshFI GGCC 1 cut(s) 205
BsiSI CCGG 1 cut(s) 23
BslFI GGGAC 1 cut(s) 62
BslI CCNNNNNNNGG 2 cut(s) 22, 23
BsmAI GTCTC 1 cut(s) 384
BsmFI GGGAC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 445
BsnI GGCC 1 cut(s) 205
Bso31I GGTCTC 1 cut(s) 384
Bsp143I GATC 2 cut(s) 175, 207
Bsp19I CCATGG 1 cut(s) 541
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 205
BspCNI CTCAG 1 cut(s) 268
BspHI TCATGA 1 cut(s) 117
BspMI ACCTGC 1 cut(s) 416
BspTNI GGTCTC 1 cut(s) 384
BssECI CCNNGG 2 cut(s) 505, 541
BssMI GATC 2 cut(s) 175, 207
BssT1I CCWWGG 2 cut(s) 505, 541
Bst2UI CCWGG 1 cut(s) 145
Bst6I CTCTTC 2 cut(s) 303, 363
BstDEI CTNAG 3 cut(s) 150, 276, 326
BstDSI CCRYGG 1 cut(s) 541
BstKTI GATC 2 cut(s) 178, 210
BstMAI GTCTC 1 cut(s) 384
BstMBI GATC 2 cut(s) 175, 207
BstMWI GCNNNNNNNGC 3 cut(s) 431, 440, 449
BstNI CCWGG 1 cut(s) 145
BstSCI CCNGG 2 cut(s) 22, 143
BstV1I GCAGC 3 cut(s) 47, 558, 561
BsuI GTATCC 1 cut(s) 265
BsuRI GGCC 1 cut(s) 205
BtgI CCRYGG 1 cut(s) 541
BveI ACCTGC 1 cut(s) 416
CaiI CAGNNNCTG 2 cut(s) 408, 452
CciI TCATGA 1 cut(s) 117
Cfr13I GGNCC 1 cut(s) 405
Csp6I GTAC 2 cut(s) 191, 228
CviAII CATG 3 cut(s) 118, 211, 542
CviQI GTAC 2 cut(s) 191, 228
DdeI CTNAG 3 cut(s) 150, 276, 326
DpnI GATC 2 cut(s) 177, 209
DpnII GATC 2 cut(s) 175, 207
Eam1104I CTCTTC 2 cut(s) 303, 363
EarI CTCTTC 2 cut(s) 303, 363
Eco130I CCWWGG 2 cut(s) 505, 541
Eco31I GGTCTC 1 cut(s) 384
Eco47I GGWCC 1 cut(s) 405
Eco57I CTGAAG 1 cut(s) 429
EcoO109I RGGNCCY 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 143
EcoT14I CCWWGG 2 cut(s) 505, 541
ErhI CCWWGG 2 cut(s) 505, 541
FaeI CATG 3 cut(s) 121, 214, 545
FaqI GGGAC 1 cut(s) 62
FatI CATG 3 cut(s) 117, 210, 541
FblI GTMKAC 1 cut(s) 376
Fnu4HI GCNGC 5 cut(s) 5, 8, 61, 547, 550
Fsp4HI GCNGC 5 cut(s) 5, 8, 61, 547, 550
FspBI CTAG 3 cut(s) 44, 225, 422
GluI GCNGC 5 cut(s) 5, 8, 61, 547, 550
GsaI CCCAGC 2 cut(s) 438, 456
GsuI CTGGAG 1 cut(s) 257
HaeIII GGCC 1 cut(s) 205
HapII CCGG 1 cut(s) 23
Hin1II CATG 3 cut(s) 121, 214, 545
HinfI GANTC 3 cut(s) 85, 140, 373
HpaII CCGG 1 cut(s) 23
HphI GGTGA 2 cut(s) 149, 482
Hpy166II GTNNAC 2 cut(s) 377, 473
Hpy188I TCNGA 3 cut(s) 38, 175, 448
Hpy188III TCNNGA 2 cut(s) 118, 236
Hpy8I GTNNAC 2 cut(s) 377, 473
HpyAV CCTTC 1 cut(s) 424
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 2 cut(s) 71, 411
HpyF10VI GCNNNNNNNGC 3 cut(s) 431, 440, 449
HpyF3I CTNAG 3 cut(s) 150, 276, 326
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 3 cut(s) 121, 214, 545
Kzo9I GATC 2 cut(s) 175, 207
Lsp1109I GCAGC 3 cut(s) 47, 558, 561
MaeI CTAG 3 cut(s) 44, 225, 422
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 1 cut(s) 299
MalI GATC 2 cut(s) 177, 209
MboI GATC 2 cut(s) 175, 207
MboII GAAGA 3 cut(s) 320, 352, 380
MluCI AATT 2 cut(s) 231, 354
MlyI GAGTC 2 cut(s) 149, 382
MnlI CCTC 2 cut(s) 50, 250
MseI TTAA 3 cut(s) 164, 200, 492
MslI CAYNNNNRTG 1 cut(s) 215
MspA1I CMGCKG 1 cut(s) 7
MspI CCGG 1 cut(s) 23
MspR9I CCNGG 2 cut(s) 24, 145
Mva1269I GAATGC 1 cut(s) 445
MvaI CCWGG 1 cut(s) 145
MwoI GCNNNNNNNGC 3 cut(s) 431, 440, 449
NciI CCSGG 1 cut(s) 24
NcoI CCATGG 1 cut(s) 541
NdeII GATC 2 cut(s) 175, 207
NlaIII CATG 3 cut(s) 121, 214, 545
NmuCI GTSAC 1 cut(s) 299
PagI TCATGA 1 cut(s) 117
PctI GAATGC 1 cut(s) 445
PfeI GAWTC 1 cut(s) 85
PfoI TCCNGGA 1 cut(s) 143
PkrI GCNGC 5 cut(s) 6, 9, 62, 548, 551
PleI GAGTC 2 cut(s) 148, 381
PpsI GAGTC 2 cut(s) 148, 381
PpuMI RGGWCCY 1 cut(s) 405
PsiI TTATAA 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 405
Psp6I CCWGG 1 cut(s) 143
PspFI CCCAGC 2 cut(s) 434, 452
PspGI CCWGG 1 cut(s) 143
PspPI GGNCC 1 cut(s) 405
PspPPI RGGWCCY 1 cut(s) 405
PstNI CAGNNNCTG 2 cut(s) 408, 452
RsaI GTAC 2 cut(s) 192, 229
RsaNI GTAC 2 cut(s) 191, 228
RseI CAYNNNNRTG 1 cut(s) 215
SaqAI TTAA 3 cut(s) 164, 200, 492
SatI GCNGC 5 cut(s) 5, 8, 61, 547, 550
Sau3AI GATC 2 cut(s) 175, 207
Sau96I GGNCC 1 cut(s) 405
SchI GAGTC 2 cut(s) 149, 382
ScrFI CCNGG 2 cut(s) 24, 145
SetI ASST 6 cut(s) 45, 135, 156, 410, 454, 554
SinI GGWCC 1 cut(s) 405
SmiMI CAYNNNNRTG 1 cut(s) 215
SpeI ACTAGT 1 cut(s) 224
Sse9I AATT 2 cut(s) 231, 354
SsiI CCGC 1 cut(s) 5
SspMI CTAG 3 cut(s) 44, 225, 422
StyD4I CCNGG 2 cut(s) 22, 143
StyI CCWWGG 2 cut(s) 505, 541
TaiI ACGT 1 cut(s) 135
TasI AATT 2 cut(s) 231, 354
TatI WGTACW 2 cut(s) 190, 227
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 1 cut(s) 85
Tru1I TTAA 3 cut(s) 164, 200, 492
Tru9I TTAA 3 cut(s) 164, 200, 492
TseFI GTSAC 1 cut(s) 299
TseI GCWGC 4 cut(s) 7, 60, 546, 549
Tsp45I GTSAC 1 cut(s) 299
TspDTI ATGAA 3 cut(s) 134, 381, 500
VpaK11BI GGWCC 1 cut(s) 405
XmiI GTMKAC 1 cut(s) 376
XspI CTAG 3 cut(s) 44, 225, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.