pycom11g04480

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001135
Physical Location & Seq
Forward (+)
3469202 .. 3472082
2881 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g04480.8

Sequence Viewer

Length: 1350 bp
ATGGCCCGTCCCCTCTGTTCGGCGCTGCCGAACGACGTCGTCTTCCGCGAGGATCGACCCTGCCCTACGCCGCCCTCGCCGTCATTGCCGACCACCAACCCCGATCCCTCCGTCATAGCCACCGAATCTTGGGCCGGAGCAGAGGAGGCAACCCAGGAGATAGTCTCAAAGATTCAGCCCACTCTGGCGGCCCACCAGACGAGGAAGGAGGTCATTCAATACGTGCAGAACCTCATCACATGTAACGTCGGGTGTCAGGTTTTTCCTTATGGGTCAGTTCCTTTGAAAACGTATCTGCCTGATGGTGATATTGACATGACGGCATTTGGCAGTGCAAACATTGATGAAGTCTTTGCAACTGATGTGTATGCAATTATGAAGGGAGAGGAGAATAACGAGGCTGGTCCATTTCATGTGAAGGATGTACATTACATTGATGCCGAGGTTAAGCTTGTCAAGTGCATTATACAAAATATAGTTGTAGATATATCCTCCAATCAGTTAGGAGGATTTAGTACACTGCACTTTCTTGAACAGGTAGATAATTATATTGGCAAAAATCATCTGTTCAAGCGGAGTATTATTCTGGTAAAAGCCTGGTGCTACTATGAGAGTCGTATTCTTGGGGCCCATCATGGTTTGATTTCAACATATGCTTTGGAAACATTAATCCTATATGTTTTCCATTTTTTTCATTCATCACTGGATGGCCCTCTGGCAGTCCTTTATAGATTTTTGGATTACTTCAGCAAATTTGACTGGGAGAACTACTGTATAAGTTTAAATGGACTAGTATGCAAATCGTCCCTGCCTGATATAGTGGTTGAGGCACCAGAAAGTGGGAGAGATGATCTACTGCTGAGTGAAGAGTTTATTAGAAATTGTGTGGAAATGTTCTCTGTTCCATCAAAGGGTTATGAGACAAACTTGCGAGCTTTTCCCCTGAAGCATCTTAATATAATAGATCCACTAAAACAGAACAACAACCTTGGGCGTAGTGTCAGCAGAAGTAACTTCTATCGAATCTGCAGTGCTTTTAAATATGGAGCTCGCAAGCTTGGTTGGATTCTTTCGTTACCAGGGGAATGCATAGCAGATGAGTTGCCCAAGTTTTTTGCGAACACTTTGGATAGGCATGGAAGCAATGGTCAGACTAATGTGCACAATAATGCGTTGTCTGGAGGTGCTGAAGCTTTGAACATTTTGTTGGAGCTGGGTGGTGATTTTGACAGGAATGAACCTGCAATATGGTCCCTTTTTCCCTGGATCTGCTACATCACCTCCTTTGTTGCCCAGTCCTCCCTTGTCACCTCAGCCATGGAAAGAGAACCCCTGGGGAGGTACGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAP_assoc

Protein Analysis

450

Amino Acids

50.2

Weight (kDa)

5.22

Isoelectric Point (pI)

36.18

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 39
Acc36I ACCTGC 1 cut(s) 1249
AccB1I GGYRCC 1 cut(s) 829
AccB7I CCANNNNNTGG 1 cut(s) 839
AccII CGCG 1 cut(s) 48
AciI CCGC 4 cut(s) 46, 71, 188, 574
AclWI GGATC 4 cut(s) 60, 98, 959, 1274
AcsI RAATTY 1 cut(s) 752
AcuI CTGAAG 3 cut(s) 730, 965, 1209
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 3 cut(s) 426, 517, 1343
AfiI CCNNNNNNNGG 5 cut(s) 19, 129, 839, 911, 1338
AflIII ACRYGT 1 cut(s) 239
AgsI TTSAA 6 cut(s) 218, 286, 533, 571, 648, 1198
AhlI ACTAGT 1 cut(s) 790
AjnI CCWGG 5 cut(s) 153, 596, 1078, 1262, 1332
AjuI GAANNNNNNNTTGG 2 cut(s) 1190, 1222
AluBI AGCT 6 cut(s) 451, 935, 1049, 1057, 1193, 1213
AluI AGCT 6 cut(s) 451, 935, 1049, 1057, 1193, 1213
Alw21I GWGCWC 2 cut(s) 1051, 1164
Alw26I GTCTC 2 cut(s) 169, 914
Alw44I GTGCAC 1 cut(s) 1160
AlwI GGATC 4 cut(s) 60, 98, 959, 1274
AoxI GGCC 5 cut(s) 3, 132, 189, 627, 709
ApaI GGGCCC 1 cut(s) 631
ApaLI GTGCAC 1 cut(s) 1160
ApeKI GCWGC 1 cut(s) 25
ApoI RAATTY 1 cut(s) 752
AseI ATTAAT 1 cut(s) 668
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 8 cut(s) 4, 132, 190, 404, 627, 628, 710, 1251
AsuHPI GGTGA 4 cut(s) 317, 1232, 1270, 1300
AvaII GGWCC 2 cut(s) 404, 1251
BaeGI GKGCMC 2 cut(s) 631, 1164
BanI GGYRCC 1 cut(s) 829
BanII GRGCYC 2 cut(s) 631, 1051
BbsI GAAGAC 1 cut(s) 34
Bbv12I GWGCWC 2 cut(s) 1051, 1164
BbvCI CCTCAGC 1 cut(s) 1312
BbvI GCAGC 1 cut(s) 12
BccI CCATC 4 cut(s) 296, 639, 701, 913
BceAI ACGGC 2 cut(s) 64, 336
BciT130I CCWGG 5 cut(s) 155, 598, 1080, 1264, 1334
BcoDI GTCTC 2 cut(s) 169, 914
BcuI ACTAGT 1 cut(s) 790
BfaI CTAG 1 cut(s) 791
BfmI CTRYAG 1 cut(s) 1027
BfoI RGCGCY 1 cut(s) 26
BfuAI ACCTGC 1 cut(s) 1249
BisI GCNGC 3 cut(s) 26, 71, 189
BlsI GCNGC 3 cut(s) 27, 72, 190
Bme1390I CCNGG 5 cut(s) 155, 598, 1080, 1264, 1334
Bme18I GGWCC 2 cut(s) 404, 1251
BmgT120I GGNCC 8 cut(s) 4, 132, 190, 404, 627, 628, 710, 1251
BmiI GGNNCC 4 cut(s) 628, 629, 831, 1253
BmrFI CCNGG 5 cut(s) 155, 598, 1080, 1264, 1334
BmrI ACTGGG 2 cut(s) 769, 1288
BmsI GCATC 2 cut(s) 427, 958
BmuI ACTGGG 2 cut(s) 769, 1288
BpiI GAAGAC 1 cut(s) 34
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpmI CTGGAG 1 cut(s) 1200
Bpu10I CCTNAGC 1 cut(s) 1312
BsaAI YACGTR 1 cut(s) 223
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 8 cut(s) 153, 441, 988, 1079, 1262, 1317, 1332, 1333
BsaXI ACNNNNNCTCC 2 cut(s) 1265, 1295
Bsc4I CCNNNNNNNGG 5 cut(s) 19, 129, 839, 911, 1338
Bse1I ACTGG 3 cut(s) 708, 764, 1294
Bse3DI GCAATG 2 cut(s) 83, 1150
BseBI CCWGG 5 cut(s) 155, 598, 1080, 1264, 1334
BseDI CCNNGG 8 cut(s) 153, 441, 988, 1079, 1262, 1317, 1332, 1333
BseGI GGATG 2 cut(s) 427, 712
BseLI CCNNNNNNNGG 5 cut(s) 19, 129, 839, 911, 1338
BseMI GCAATG 2 cut(s) 83, 1150
BseMII CTCAG 2 cut(s) 851, 1326
BseNI ACTGG 3 cut(s) 708, 764, 1294
BseRI GAGGAG 2 cut(s) 158, 401
BseSI GKGCMC 2 cut(s) 631, 1164
BseXI GCAGC 1 cut(s) 12
BseYI CCCAGC 1 cut(s) 1213
BsgI GTGCAG 2 cut(s) 245, 506
Bsh1236I CGCG 1 cut(s) 48
BshFI GGCC 5 cut(s) 5, 134, 191, 629, 711
BshNI GGYRCC 1 cut(s) 829
BsiHKAI GWGCWC 2 cut(s) 1051, 1164
BsiSI CCGG 1 cut(s) 135
BslFI GGGAC 2 cut(s) 790, 1237
BslI CCNNNNNNNGG 5 cut(s) 19, 129, 839, 911, 1338
BsmAI GTCTC 2 cut(s) 169, 914
BsmFI GGGAC 2 cut(s) 790, 1237
BsmI GAATGC 1 cut(s) 1091
BsnI GGCC 5 cut(s) 5, 134, 191, 629, 711
Bsp120I GGGCCC 1 cut(s) 627
Bsp1286I GDGCHC 3 cut(s) 631, 1051, 1164
Bsp1407I TGTACA 1 cut(s) 424
Bsp143I GATC 5 cut(s) 52, 103, 850, 964, 1266
Bsp19I CCATGG 1 cut(s) 1317
BspACI CCGC 4 cut(s) 46, 71, 188, 574
BspANI GGCC 5 cut(s) 5, 134, 191, 629, 711
BspCNI CTCAG 2 cut(s) 852, 1325
BspFNI CGCG 1 cut(s) 48
BspLI GGNNCC 4 cut(s) 628, 629, 831, 1253
BspMAI CTGCAG 1 cut(s) 1031
BspMI ACCTGC 1 cut(s) 1249
BspPI GGATC 4 cut(s) 60, 98, 959, 1274
BspT107I GGYRCC 1 cut(s) 829
BsrDI GCAATG 2 cut(s) 83, 1150
BsrGI TGTACA 1 cut(s) 424
BsrI ACTGG 3 cut(s) 708, 764, 1294
BssECI CCNNGG 8 cut(s) 153, 441, 988, 1079, 1262, 1317, 1332, 1333
BssMI GATC 5 cut(s) 52, 103, 850, 964, 1266
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 2 cut(s) 988, 1317
Bst2UI CCWGG 5 cut(s) 155, 598, 1080, 1264, 1334
Bst4CI ACNGT 1 cut(s) 773
Bst6I CTCTTC 1 cut(s) 861
BstACI GRCGYC 1 cut(s) 36
BstAUI TGTACA 1 cut(s) 424
BstBAI YACGTR 1 cut(s) 223
BstC8I GCNNGC 3 cut(s) 933, 1051, 1055
BstDEI CTNAG 2 cut(s) 860, 1312
BstDSI CCRYGG 1 cut(s) 1317
BstF5I GGATG 2 cut(s) 427, 712
BstFNI CGCG 1 cut(s) 48
BstH2I RGCGCY 1 cut(s) 26
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 5 cut(s) 55, 106, 853, 967, 1269
BstMAI GTCTC 2 cut(s) 169, 914
BstMBI GATC 5 cut(s) 52, 103, 850, 964, 1266
BstMWI GCNNNNNNNGC 3 cut(s) 76, 85, 146
BstNI CCWGG 5 cut(s) 155, 598, 1080, 1264, 1334
BstNSI RCATGY 1 cut(s) 243
BstSCI CCNGG 5 cut(s) 153, 596, 1078, 1262, 1332
BstSFI CTRYAG 1 cut(s) 1027
BstSLI GKGCMC 2 cut(s) 631, 1164
BstUI CGCG 1 cut(s) 48
BstV1I GCAGC 1 cut(s) 12
BstV2I GAAGAC 1 cut(s) 34
BstX2I RGATCY 2 cut(s) 964, 1266
BstYI RGATCY 2 cut(s) 964, 1266
BsuRI GGCC 5 cut(s) 5, 134, 191, 629, 711
BtgI CCRYGG 1 cut(s) 1317
BtsCI GGATG 2 cut(s) 427, 712
BtsI GCAGTG 3 cut(s) 337, 518, 1036
BtsIMutI CAGTG 4 cut(s) 337, 518, 701, 1036
BveI ACCTGC 1 cut(s) 1249
Cac8I GCNNGC 3 cut(s) 933, 1051, 1055
CfoI GCGC 1 cut(s) 25
Cfr13I GGNCC 8 cut(s) 4, 132, 190, 404, 627, 628, 710, 1251
Csp6I GTAC 3 cut(s) 425, 516, 1342
CviAII CATG 6 cut(s) 240, 316, 413, 635, 1136, 1318
CviQI GTAC 3 cut(s) 425, 516, 1342
DdeI CTNAG 2 cut(s) 860, 1312
DpnI GATC 5 cut(s) 54, 105, 852, 966, 1268
DpnII GATC 5 cut(s) 52, 103, 850, 964, 1266
DraI TTTAAA 2 cut(s) 783, 1039
Eam1104I CTCTTC 1 cut(s) 861
EarI CTCTTC 1 cut(s) 861
Ecl136II GAGCTC 1 cut(s) 1049
Eco130I CCWWGG 2 cut(s) 988, 1317
Eco24I GRGCYC 2 cut(s) 631, 1051
Eco47I GGWCC 2 cut(s) 404, 1251
Eco53kI GAGCTC 1 cut(s) 1049
Eco57I CTGAAG 3 cut(s) 730, 965, 1209
EcoICRI GAGCTC 1 cut(s) 1049
EcoO109I RGGNCCY 1 cut(s) 627
EcoRII CCWGG 5 cut(s) 153, 596, 1078, 1262, 1332
EcoT14I CCWWGG 2 cut(s) 988, 1317
EcoT22I ATGCAT 1 cut(s) 1091
EcoT38I GRGCYC 2 cut(s) 631, 1051
ErhI CCWWGG 2 cut(s) 988, 1317
FaeI CATG 6 cut(s) 243, 319, 416, 638, 1139, 1321
FaqI GGGAC 2 cut(s) 790, 1237
FatI CATG 6 cut(s) 239, 315, 412, 634, 1135, 1317
FauNDI CATATG 1 cut(s) 652
Fnu4HI GCNGC 3 cut(s) 26, 71, 189
FokI GGATG 2 cut(s) 434, 719
FriOI GRGCYC 2 cut(s) 631, 1051
Fsp4HI GCNGC 3 cut(s) 26, 71, 189
FspBI CTAG 1 cut(s) 791
GlaI GCGC 1 cut(s) 24
GluI GCNGC 3 cut(s) 26, 71, 189
GsaI CCCAGC 1 cut(s) 1217
GsuI CTGGAG 1 cut(s) 1200
HaeII RGCGCY 1 cut(s) 26
HaeIII GGCC 5 cut(s) 5, 134, 191, 629, 711
HapII CCGG 1 cut(s) 135
HhaI GCGC 1 cut(s) 25
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 6 cut(s) 243, 319, 416, 638, 1139, 1321
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HindIII AAGCTT 3 cut(s) 449, 1055, 1191
HinfI GANTC 5 cut(s) 125, 172, 613, 1023, 1066
HpaII CCGG 1 cut(s) 135
HphI GGTGA 4 cut(s) 317, 1232, 1270, 1300
Hpy166II GTNNAC 2 cut(s) 518, 1162
Hpy188I TCNGA 1 cut(s) 1152
Hpy188III TCNNGA 2 cut(s) 530, 1179
Hpy8I GTNNAC 2 cut(s) 518, 1162
Hpy99I CGWCG 3 cut(s) 38, 41, 251
HpyAV CCTTC 3 cut(s) 199, 373, 412
HpyCH4III ACNGT 1 cut(s) 773
HpyCH4IV ACGT 4 cut(s) 36, 222, 246, 290
HpyF10VI GCNNNNNNNGC 3 cut(s) 76, 85, 146
HpyF3I CTNAG 2 cut(s) 860, 1312
HpySE526I ACGT 4 cut(s) 36, 222, 246, 290
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 6 cut(s) 243, 319, 416, 638, 1139, 1321
HspAI GCGC 1 cut(s) 23
Kzo9I GATC 5 cut(s) 52, 103, 850, 964, 1266
LmnI GCTCC 3 cut(s) 137, 1046, 1210
Lsp1109I GCAGC 1 cut(s) 12
LweI GCATC 2 cut(s) 427, 958
MaeI CTAG 1 cut(s) 791
MaeII ACGT 4 cut(s) 36, 222, 246, 290
MaeIII GTNAC 4 cut(s) 242, 1010, 1074, 1306
MalI GATC 5 cut(s) 54, 105, 852, 966, 1268
MboI GATC 5 cut(s) 52, 103, 850, 964, 1266
MboII GAAGA 2 cut(s) 34, 878
MflI RGATCY 2 cut(s) 964, 1266
MhlI GDGCHC 3 cut(s) 631, 1051, 1164
MluCI AATT 4 cut(s) 372, 544, 752, 880
MlyI GAGTC 1 cut(s) 622
MmeI TCCRAC 2 cut(s) 1043, 1188
Mph1103I ATGCAT 1 cut(s) 1091
MseI TTAA 5 cut(s) 447, 668, 782, 954, 1038
MslI CAYNNNNRTG 1 cut(s) 1167
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 5 cut(s) 155, 598, 1080, 1264, 1334
Mva1269I GAATGC 1 cut(s) 1091
MvaI CCWGG 5 cut(s) 155, 598, 1080, 1264, 1334
MvnI CGCG 1 cut(s) 48
MwoI GCNNNNNNNGC 3 cut(s) 76, 85, 146
NcoI CCATGG 1 cut(s) 1317
NdeI CATATG 1 cut(s) 652
NdeII GATC 5 cut(s) 52, 103, 850, 964, 1266
NlaIII CATG 6 cut(s) 243, 319, 416, 638, 1139, 1321
NlaIV GGNNCC 4 cut(s) 628, 629, 831, 1253
NmeAIII GCCGAG 1 cut(s) 466
NmuCI GTSAC 1 cut(s) 1306
NsiI ATGCAT 1 cut(s) 1091
NspI RCATGY 1 cut(s) 243
PasI CCCWGGG 1 cut(s) 1333
PciI ACATGT 1 cut(s) 239
PcsI WCGNNNNNNNCGW 2 cut(s) 26, 45
PctI GAATGC 1 cut(s) 1091
PfeI GAWTC 4 cut(s) 125, 172, 1023, 1066
PflFI GACNNNGTC 1 cut(s) 38
PflMI CCANNNNNTGG 1 cut(s) 839
PkrI GCNGC 3 cut(s) 27, 72, 190
PleI GAGTC 1 cut(s) 621
PpsI GAGTC 1 cut(s) 621
Ppu21I YACGTR 1 cut(s) 223
PscI ACATGT 1 cut(s) 239
PshBI ATTAAT 1 cut(s) 668
Psp124BI GAGCTC 1 cut(s) 1051
Psp6I CCWGG 5 cut(s) 153, 596, 1078, 1262, 1332
PspFI CCCAGC 1 cut(s) 1213
PspGI CCWGG 5 cut(s) 153, 596, 1078, 1262, 1332
PspN4I GGNNCC 4 cut(s) 628, 629, 831, 1253
PspOMI GGGCCC 1 cut(s) 627
PspPI GGNCC 8 cut(s) 4, 132, 190, 404, 627, 628, 710, 1251
PstI CTGCAG 1 cut(s) 1031
PsuI RGATCY 2 cut(s) 964, 1266
PsyI GACNNNGTC 1 cut(s) 38
RsaI GTAC 3 cut(s) 426, 517, 1343
RsaNI GTAC 3 cut(s) 425, 516, 1342
RseI CAYNNNNRTG 1 cut(s) 1167
SacI GAGCTC 1 cut(s) 1051
SaqAI TTAA 5 cut(s) 447, 668, 782, 954, 1038
SatI GCNGC 3 cut(s) 26, 71, 189
Sau3AI GATC 5 cut(s) 52, 103, 850, 964, 1266
Sau96I GGNCC 8 cut(s) 4, 132, 190, 404, 627, 628, 710, 1251
SchI GAGTC 1 cut(s) 622
ScrFI CCNGG 5 cut(s) 155, 598, 1080, 1264, 1334
SduI GDGCHC 3 cut(s) 631, 1051, 1164
SfaNI GCATC 2 cut(s) 427, 958
SfcI CTRYAG 1 cut(s) 1027
SinI GGWCC 2 cut(s) 404, 1251
SmiMI CAYNNNNRTG 1 cut(s) 1167
SpeI ACTAGT 1 cut(s) 790
Sse9I AATT 4 cut(s) 372, 544, 752, 880
SsiI CCGC 4 cut(s) 46, 71, 188, 574
SspMI CTAG 1 cut(s) 791
SstI GAGCTC 1 cut(s) 1051
StyD4I CCNGG 5 cut(s) 153, 596, 1078, 1262, 1332
StyI CCWWGG 2 cut(s) 988, 1317
TaaI ACNGT 1 cut(s) 773
TaiI ACGT 4 cut(s) 39, 225, 249, 293
TaqI TCGA 2 cut(s) 55, 1021
TasI AATT 4 cut(s) 372, 544, 752, 880
TatI WGTACW 2 cut(s) 424, 515
TauI GCSGC 2 cut(s) 73, 191
TfiI GAWTC 4 cut(s) 125, 172, 1023, 1066
Tru1I TTAA 5 cut(s) 447, 668, 782, 954, 1038
Tru9I TTAA 5 cut(s) 447, 668, 782, 954, 1038
TscAI CASTG 4 cut(s) 337, 525, 708, 1036
TseFI GTSAC 1 cut(s) 1306
TseI GCWGC 1 cut(s) 25
Tsp45I GTSAC 1 cut(s) 1306
TspDTI ATGAA 6 cut(s) 360, 392, 401, 683, 687, 1251
TspGWI ACGGA 1 cut(s) 100
TspRI CASTG 4 cut(s) 337, 525, 708, 1036
Tth111I GACNNNGTC 1 cut(s) 38
Van91I CCANNNNNTGG 1 cut(s) 839
VneI GTGCAC 1 cut(s) 1160
VpaK11BI GGWCC 2 cut(s) 404, 1251
VspI ATTAAT 1 cut(s) 668
XapI RAATTY 1 cut(s) 752
XceI RCATGY 1 cut(s) 243
XspI CTAG 1 cut(s) 791
ZraI GACGTC 1 cut(s) 37
Zsp2I ATGCAT 1 cut(s) 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.