Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001135

Physical Location & Seq

Genomic Coordinates Forward (+)

4250741 .. 4255007

Gene Structure 4267 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1053200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGGCCCGCCTCCACTGTTCGGCGCGGCCGAACGACGGCGTTTTCCGCGAGGATCGACCCTGCCCTTCGCCGTCATTGCCGACCTCCAACCCCGATCCCTCCTCCATAGCCCTTGAGTCTTGGGCCGGAGCCGAGCAGGCAATCCAGGAGATAGTCTCAAAGATTCAGCCTACTCTGGCGGCCCACCAGACGAGGACGGAGGTCATTCAATATGTGCAGAACCTCATCACATGTAACGTCGGGTGTCAGGTTTTTCCTTATGGGTCAGTTCCATTGAAAACGTATCTGCCCGATGGAGATATTGATATGACGGCATTTGGCAGTGCAAATATTGATAAAGTCTTTGCAACTGATGTCTATGCAATTATGAAGGGCGAGGAGAACAACGAAGCTTCTCCGTTTCATGTGAAGGATGTACATTACATTGATGCCGAGGTTAAGCTTGTGAAGTGCATTATACAAAATATAGTTGTAGATATATCCTCCAATCAGTTAGGAGGATTTAGTACCCTGCACTTTCTTGAACAGGTTGATAATTATATTGGCAAAAATCATCTCTTCAAGCGGAGTATTATTCTTATAAAAGCCTGGTGCTACTATGAGAGTCGTATTCTTGGGGCCCATCATGGGTTGATTTCAACATATGCTTTGGAAATATTAATCCTATATGTTTTCCATTTTTTCCATTCGTCACTGGATGGCCCTCTGGCAGTCCTTTATAAATTTTTGGATTACTTAAGCAAATTTGACTGGGAGAACTATTGCATAAGTTTAAACGGACCAGTATGCAAATCATCCTTGCCTGATATAGTGGCTAAGGCACCTGAAAATGGGAGAGATGATCTACTGCTGAGTGAAGAATTTATTAGAAATTGTGTGGAAATGTTCTCTGTTCCAGCAAAGGGGTATGAGACAAACTTGCGAGCTTTTTCCCTGAAGCATCTTAATATAATAGATCCACTAAAGCAGGACAACAACCTTGGGCGAAGTGTCAGCAGAAGTAACTTCTATCGAATATGCAGTGCCTTTAAATATGGAGCTCACAAGCTTGGTTGGATTCTTTCATTACCGGGGGAATGCATAGCAGATGAGCTGCCCAAGTTTTTCGCAAATACTTTGGATAGACATGGAAGCAATGGTCAGATTAATGTGCACAATAATGCCTTGTCTGGAAGTTCTGAATCTTTGAACATTTTGTTGGAGCTTGGTGGAGATTTCGACAGTCATGGAATGAACCTGCAATATGGTCCCTTTTTCCCGGGATCTGCTGCATTACCTCCTTTATTTCCCAGTCTTCCCTTGTCACCTCAGTTATGGAAAAAGAACCCATGGGGAGGTACGTCTGAAGCCCTTCAGTTCCATCAGAATACTAATTCTCAAACTAACATACATGGTGTTGACTGGGGAGCACATGCGTACCATGCAAATGATCCCACTTTCTCCGTTGCTGCTTTCAGAGGAGAGAAAAGGAAACCCCGAGGGACCGGAACGTACATCCCAAATCCGTCTTTTCAGAATTACCGTTCCTTAAAAGGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PAP_assoc

Protein Analysis

513

Amino Acids

56.98

Weight (kDa)

6.25

Isoelectric Point (pI)

38.14

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTPAP-like_central	PF22600	46 - 175	1.3e-07	Poly(A) RNA polymerase, mitochondrial-like, central palm domain
PAP-OAS1	PF26180	181 - 373	2.6e-98	PAP/OAS1 substrate-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001135)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40520 AT2G40520 AT2G40520 AT2G40520 AT2G40520 AT2G40520 AT3G56320 AT3G56320 AT3G56320 AT3G56320 AT3G56320 AT3G56320 AT3G56320
fragaria_vesca	FvH4_3g40330 FvH4_3g40330 FvH4_3g40330 FvH4_7g25750 FvH4_7g25750 FvH4_7g25750 FvH4_7g25750
malus_domestica	MD01G1165200.v1.1 MD03G1053200.v1.1 MD07G1232500.v1.1 MD11G1054900.v1.1
prunus_persica	Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.6G042500_v2.0.a1 Prupe.6G042500_v2.0.a1
pyrus_communis	pycom01g17910 pycom03g04230 pycom11g04480
rosa_chinensis	RchiOBHm_Chr1g0372011 RchiOBHm_Chr5g0072681
rosa_laevigata	RLG00000026928 RLG00000036325
rosa_multiflora	Rmu_sc0001755.1_g000036 Rmu_sc0003160.1_g000034
rosa_roxburghii	Rroxscaffold_1G00008460
rosa_rugosa	Rorug01G0367900 Rorug01G0368000 Rorug01G0368100 Rorug01G0368200 Rorug01G0368300 Rorug05G0419200 Rorug05G0419300
rosa_samantha	Rh1AG377600 Rh1BG341000 Rh1CG354200 Rh1DG372000 Rh5AG476200 Rh5BG496400 Rh5CG520200 Rh5DG508800
rosa_wichuraiana	Rw1G033220 Rw5G044220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	581, 720
Acc36I	ACCTGC	1 cut(s)	1245
AccB1I	GGYRCC	1 cut(s)	820
AccII	CGCG	2 cut(s)	25, 48
AciI	CCGC	5 cut(s)	7, 25, 46, 179, 565
AclWI	GGATC	5 cut(s)	60, 89, 950, 1270, 1424
AcoI	YGGCCR	1 cut(s)	26
AcsI	RAATTY	3 cut(s)	722, 743, 860
AcuI	CTGAAG	3 cut(s)	956, 1337, 1365
AfaI	GTAC	5 cut(s)	417, 508, 1339, 1418, 1493
AfiI	CCNNNNNNNGG	6 cut(s)	19, 35, 627, 830, 902, 1334
AflII	CTTAAG	1 cut(s)	736
AflIII	ACRYGT	1 cut(s)	230
AgsI	TTSAA	6 cut(s)	209, 277, 524, 562, 639, 1189
AjnI	CCWGG	2 cut(s)	144, 587
AjuI	GAANNNNNNNTTGG	2 cut(s)	1181, 1213
AluBI	AGCT	7 cut(s)	392, 442, 926, 1040, 1048, 1093, 1204
AluI	AGCT	7 cut(s)	392, 442, 926, 1040, 1048, 1093, 1204
Alw21I	GWGCWC	3 cut(s)	1042, 1155, 1411
Alw26I	GTCTC	2 cut(s)	160, 905
Alw44I	GTGCAC	1 cut(s)	1151
AlwI	GGATC	5 cut(s)	60, 89, 950, 1270, 1424
Ama87I	CYCGRG	2 cut(s)	1258, 1476
AoxI	GGCC	6 cut(s)	3, 26, 123, 180, 618, 700
ApaI	GGGCCC	1 cut(s)	622
ApaLI	GTGCAC	1 cut(s)	1151
ApeKI	GCWGC	3 cut(s)	1093, 1268, 1448
ApoI	RAATTY	3 cut(s)	722, 743, 860
AseI	ATTAAT	2 cut(s)	659, 1146
Asp700I	GAANNNNTTC	1 cut(s)	1350
AspLEI	GCGC	1 cut(s)	25
AspS9I	GGNCC	9 cut(s)	4, 123, 181, 618, 619, 701, 779, 1247, 1482
AsuC2I	CCSGG	3 cut(s)	1071, 1259, 1260
AsuHPI	GGTGA	1 cut(s)	1296
AvaI	CYCGRG	2 cut(s)	1258, 1476
AvaII	GGWCC	3 cut(s)	779, 1247, 1482
BaeGI	GKGCMC	2 cut(s)	622, 1155
BaeI	ACNNNNGTAYC	2 cut(s)	1400, 1433
BanI	GGYRCC	1 cut(s)	820
BanII	GRGCYC	2 cut(s)	622, 1042
BbsI	GAAGAC	1 cut(s)	1286
Bbv12I	GWGCWC	3 cut(s)	1042, 1155, 1411
BbvI	GCAGC	3 cut(s)	1080, 1255, 1435
BccI	CCATC	4 cut(s)	287, 630, 692, 1368
BceAI	ACGGC	3 cut(s)	52, 55, 327
BciT130I	CCWGG	2 cut(s)	146, 589
BcnI	CCSGG	3 cut(s)	1071, 1259, 1260
BcoDI	GTCTC	2 cut(s)	160, 905
BfrI	CTTAAG	1 cut(s)	736
BfuAI	ACCTGC	1 cut(s)	1245
BglI	GCCNNNNNGGC	1 cut(s)	137
BisI	GCNGC	5 cut(s)	26, 180, 1094, 1269, 1449
BlsI	GCNGC	5 cut(s)	27, 181, 1095, 1270, 1450
Bme1390I	CCNGG	5 cut(s)	146, 589, 1071, 1259, 1260
Bme18I	GGWCC	3 cut(s)	779, 1247, 1482
BmeT110I	CYCGRG	2 cut(s)	1258, 1476
BmgT120I	GGNCC	9 cut(s)	4, 123, 181, 618, 619, 701, 779, 1247, 1482
BmiI	GGNNCC	6 cut(s)	130, 619, 620, 822, 1249, 1483
BmrFI	CCNGG	5 cut(s)	146, 589, 1071, 1259, 1260
BmrI	ACTGGG	3 cut(s)	760, 1284, 1411
BmsI	GCATC	2 cut(s)	418, 949
BmuI	ACTGGG	3 cut(s)	760, 1284, 1411
BoxI	GACNNNNGTC	1 cut(s)	200
BpiI	GAAGAC	1 cut(s)	1286
BplI	GAGNNNNNCTC	2 cut(s)	140, 172
Bpu10I	CCTNAGC	1 cut(s)	816
BpuEI	CTTGAG	1 cut(s)	134
BpuMI	CCSGG	3 cut(s)	1071, 1259, 1260
BsaJI	CCNNGG	6 cut(s)	432, 979, 1070, 1258, 1328, 1477
BsaWI	WCCGGW	1 cut(s)	1484
Bsc4I	CCNNNNNNNGG	6 cut(s)	19, 35, 627, 830, 902, 1334
Bse1I	ACTGG	5 cut(s)	699, 755, 782, 1290, 1406
Bse3DI	GCAATG	2 cut(s)	74, 1141
BseBI	CCWGG	2 cut(s)	146, 589
BseDI	CCNNGG	6 cut(s)	432, 979, 1070, 1258, 1328, 1477
BseGI	GGATG	4 cut(s)	418, 703, 794, 1494
BseLI	CCNNNNNNNGG	6 cut(s)	19, 35, 627, 830, 902, 1334
BseMI	GCAATG	2 cut(s)	74, 1141
BseMII	CTCAG	2 cut(s)	842, 1322
BseNI	ACTGG	5 cut(s)	699, 755, 782, 1290, 1406
BseRI	GAGGAG	3 cut(s)	91, 392, 1473
BseSI	GKGCMC	2 cut(s)	622, 1155
BseX3I	CGGCCG	1 cut(s)	26
BseXI	GCAGC	3 cut(s)	1080, 1255, 1435
BsgI	GTGCAG	2 cut(s)	236, 497
Bsh1236I	CGCG	2 cut(s)	25, 48
Bsh1285I	CGRYCG	1 cut(s)	29
BshFI	GGCC	6 cut(s)	5, 28, 125, 182, 620, 702
BshNI	GGYRCC	1 cut(s)	820
BsiEI	CGRYCG	1 cut(s)	29
BsiHKAI	GWGCWC	3 cut(s)	1042, 1155, 1411
BsiHKCI	CYCGRG	2 cut(s)	1258, 1476
BsiSI	CCGG	4 cut(s)	126, 1070, 1259, 1485
BslFI	GGGAC	2 cut(s)	1233, 1495
BslI	CCNNNNNNNGG	6 cut(s)	19, 35, 627, 830, 902, 1334
BsmAI	GTCTC	2 cut(s)	160, 905
BsmFI	GGGAC	2 cut(s)	1233, 1495
BsmI	GAATGC	1 cut(s)	1082
BsnI	GGCC	6 cut(s)	5, 28, 125, 182, 620, 702
BsoBI	CYCGRG	2 cut(s)	1258, 1476
Bsp120I	GGGCCC	1 cut(s)	618
Bsp1286I	GDGCHC	4 cut(s)	622, 1042, 1155, 1411
Bsp1407I	TGTACA	1 cut(s)	415
Bsp143I	GATC	6 cut(s)	52, 94, 841, 955, 1262, 1429
Bsp19I	CCATGG	1 cut(s)	1328
BspACI	CCGC	5 cut(s)	7, 25, 46, 179, 565
BspANI	GGCC	6 cut(s)	5, 28, 125, 182, 620, 702
BspCNI	CTCAG	2 cut(s)	843, 1321
BspFNI	CGCG	2 cut(s)	25, 48
BspLI	GGNNCC	6 cut(s)	130, 619, 620, 822, 1249, 1483
BspMI	ACCTGC	1 cut(s)	1245
BspPI	GGATC	5 cut(s)	60, 89, 950, 1270, 1424
BspT107I	GGYRCC	1 cut(s)	820
BspTI	CTTAAG	1 cut(s)	736
BsrDI	GCAATG	2 cut(s)	74, 1141
BsrGI	TGTACA	1 cut(s)	415
BsrI	ACTGG	5 cut(s)	699, 755, 782, 1290, 1406
BssECI	CCNNGG	6 cut(s)	432, 979, 1070, 1258, 1328, 1477
BssMI	GATC	6 cut(s)	52, 94, 841, 955, 1262, 1429
BssT1I	CCWWGG	2 cut(s)	979, 1328
Bst2UI	CCWGG	2 cut(s)	146, 589
Bst4CI	ACNGT	3 cut(s)	17, 1223, 1523
Bst6I	CTCTTC	1 cut(s)	563
BstAFI	CTTAAG	1 cut(s)	736
BstAUI	TGTACA	1 cut(s)	415
BstC8I	GCNNGC	3 cut(s)	7, 138, 924
BstDEI	CTNAG	3 cut(s)	816, 851, 1308
BstDSI	CCRYGG	1 cut(s)	1328
BstF5I	GGATG	4 cut(s)	418, 703, 794, 1494
BstFNI	CGCG	2 cut(s)	25, 48
BstHHI	GCGC	1 cut(s)	25
BstKTI	GATC	6 cut(s)	55, 97, 844, 958, 1265, 1432
BstMAI	GTCTC	2 cut(s)	160, 905
BstMBI	GATC	6 cut(s)	52, 94, 841, 955, 1262, 1429
BstMCI	CGRYCG	1 cut(s)	29
BstMWI	GCNNNNNNNGC	4 cut(s)	45, 76, 137, 1421
BstNI	CCWGG	2 cut(s)	146, 589
BstNSI	RCATGY	2 cut(s)	234, 1415
BstPAI	GACNNNNGTC	1 cut(s)	200
BstSCI	CCNGG	5 cut(s)	144, 587, 1069, 1257, 1258
BstSLI	GKGCMC	2 cut(s)	622, 1155
BstUI	CGCG	2 cut(s)	25, 48
BstV1I	GCAGC	3 cut(s)	1080, 1255, 1435
BstV2I	GAAGAC	1 cut(s)	1286
BstX2I	RGATCY	2 cut(s)	955, 1262
BstYI	RGATCY	2 cut(s)	955, 1262
BstZI	CGGCCG	1 cut(s)	26
BsuRI	GGCC	6 cut(s)	5, 28, 125, 182, 620, 702
BtgI	CCRYGG	1 cut(s)	1328
BtsCI	GGATG	4 cut(s)	418, 703, 794, 1494
BtsI	GCAGTG	2 cut(s)	328, 1027
BtsIMutI	CAGTG	4 cut(s)	13, 328, 692, 1027
BveI	ACCTGC	1 cut(s)	1245
Cac8I	GCNNGC	3 cut(s)	7, 138, 924
CfoI	GCGC	1 cut(s)	25
Cfr13I	GGNCC	9 cut(s)	4, 123, 181, 618, 619, 701, 779, 1247, 1482
Cfr9I	CCCGGG	1 cut(s)	1258
Csp6I	GTAC	5 cut(s)	416, 507, 1338, 1417, 1492
CviAII	CATG	9 cut(s)	231, 404, 626, 1127, 1226, 1329, 1391, 1412, 1421
CviQI	GTAC	5 cut(s)	416, 507, 1338, 1417, 1492
DdeI	CTNAG	3 cut(s)	816, 851, 1308
DpnI	GATC	6 cut(s)	54, 96, 843, 957, 1264, 1431
DpnII	GATC	6 cut(s)	52, 94, 841, 955, 1262, 1429
DraI	TTTAAA	2 cut(s)	774, 1030
EaeI	YGGCCR	1 cut(s)	26
EagI	CGGCCG	1 cut(s)	26
Eam1104I	CTCTTC	1 cut(s)	563
EarI	CTCTTC	1 cut(s)	563
Ecl136II	GAGCTC	1 cut(s)	1040
EclXI	CGGCCG	1 cut(s)	26
Eco130I	CCWWGG	2 cut(s)	979, 1328
Eco24I	GRGCYC	2 cut(s)	622, 1042
Eco47I	GGWCC	3 cut(s)	779, 1247, 1482
Eco52I	CGGCCG	1 cut(s)	26
Eco53kI	GAGCTC	1 cut(s)	1040
Eco57I	CTGAAG	3 cut(s)	956, 1337, 1365
Eco88I	CYCGRG	2 cut(s)	1258, 1476
EcoICRI	GAGCTC	1 cut(s)	1040
EcoO109I	RGGNCCY	1 cut(s)	618
EcoRII	CCWGG	2 cut(s)	144, 587
EcoT14I	CCWWGG	2 cut(s)	979, 1328
EcoT22I	ATGCAT	1 cut(s)	1082
EcoT38I	GRGCYC	2 cut(s)	622, 1042
ErhI	CCWWGG	2 cut(s)	979, 1328
FaeI	CATG	9 cut(s)	234, 407, 629, 1130, 1229, 1332, 1394, 1415, 1424
FaqI	GGGAC	2 cut(s)	1233, 1495
FatI	CATG	9 cut(s)	230, 403, 625, 1126, 1225, 1328, 1390, 1411, 1420
FauI	CCCGC	1 cut(s)	14
FauNDI	CATATG	1 cut(s)	643
Fnu4HI	GCNGC	5 cut(s)	26, 180, 1094, 1269, 1449
FokI	GGATG	4 cut(s)	425, 710, 781, 1481
FriOI	GRGCYC	2 cut(s)	622, 1042
Fsp4HI	GCNGC	5 cut(s)	26, 180, 1094, 1269, 1449
GlaI	GCGC	1 cut(s)	24
GluI	GCNGC	5 cut(s)	26, 180, 1094, 1269, 1449
HaeIII	GGCC	6 cut(s)	5, 28, 125, 182, 620, 702
HapII	CCGG	4 cut(s)	126, 1070, 1259, 1485
HhaI	GCGC	1 cut(s)	25
Hin1II	CATG	9 cut(s)	234, 407, 629, 1130, 1229, 1332, 1394, 1415, 1424
Hin6I	GCGC	1 cut(s)	23
HinP1I	GCGC	1 cut(s)	23
HincII	GTYRAC	1 cut(s)	1399
HindII	GTYRAC	1 cut(s)	1399
HindIII	AAGCTT	3 cut(s)	390, 440, 1046
HinfI	GANTC	5 cut(s)	116, 163, 604, 1057, 1181
HpaII	CCGG	4 cut(s)	126, 1070, 1259, 1485
HphI	GGTGA	1 cut(s)	1296
Hpy166II	GTNNAC	2 cut(s)	1153, 1399
Hpy188I	TCNGA	6 cut(s)	1143, 1180, 1345, 1365, 1457, 1515
Hpy188III	TCNNGA	2 cut(s)	521, 1170
Hpy8I	GTNNAC	2 cut(s)	1153, 1399
Hpy99I	CGWCG	2 cut(s)	38, 242
HpyAV	CCTTC	4 cut(s)	75, 364, 403, 1361
HpyCH4III	ACNGT	3 cut(s)	17, 1223, 1523
HpyCH4IV	ACGT	4 cut(s)	237, 281, 1340, 1490
HpyF10VI	GCNNNNNNNGC	4 cut(s)	45, 76, 137, 1421
HpyF3I	CTNAG	3 cut(s)	816, 851, 1308
HpySE526I	ACGT	4 cut(s)	237, 281, 1340, 1490
Hsp92II	CATG	9 cut(s)	234, 407, 629, 1130, 1229, 1332, 1394, 1415, 1424
HspAI	GCGC	1 cut(s)	23
Kzo9I	GATC	6 cut(s)	52, 94, 841, 955, 1262, 1429
LmnI	GCTCC	4 cut(s)	128, 1037, 1201, 1406
Lsp1109I	GCAGC	3 cut(s)	1080, 1255, 1435
LweI	GCATC	2 cut(s)	418, 949
MaeII	ACGT	4 cut(s)	237, 281, 1340, 1490
MaeIII	GTNAC	4 cut(s)	233, 690, 1001, 1302
MalI	GATC	6 cut(s)	54, 96, 843, 957, 1264, 1431
MboI	GATC	6 cut(s)	52, 94, 841, 955, 1262, 1429
MboII	GAAGA	3 cut(s)	550, 869, 1286
MflI	RGATCY	2 cut(s)	955, 1262
MhlI	GDGCHC	4 cut(s)	622, 1042, 1155, 1411
MluCI	AATT	8 cut(s)	363, 535, 722, 743, 860, 871, 1372, 1516
MlyI	GAGTC	2 cut(s)	125, 613
MmeI	TCCRAC	3 cut(s)	111, 1034, 1179
Mph1103I	ATGCAT	1 cut(s)	1082
MroXI	GAANNNNTTC	1 cut(s)	1350
MseI	TTAA	8 cut(s)	438, 659, 737, 773, 945, 1029, 1146, 1529
MslI	CAYNNNNRTG	2 cut(s)	1158, 1425
MspCI	CTTAAG	1 cut(s)	736
MspI	CCGG	4 cut(s)	126, 1070, 1259, 1485
MspR9I	CCNGG	5 cut(s)	146, 589, 1071, 1259, 1260
MssI	GTTTAAAC	1 cut(s)	774
Mva1269I	GAATGC	1 cut(s)	1082
MvaI	CCWGG	2 cut(s)	146, 589
MvnI	CGCG	2 cut(s)	25, 48
MwoI	GCNNNNNNNGC	4 cut(s)	45, 76, 137, 1421
NciI	CCSGG	3 cut(s)	1071, 1259, 1260
NcoI	CCATGG	1 cut(s)	1328
NdeI	CATATG	1 cut(s)	643
NdeII	GATC	6 cut(s)	52, 94, 841, 955, 1262, 1429
NlaIII	CATG	9 cut(s)	234, 407, 629, 1130, 1229, 1332, 1394, 1415, 1424
NlaIV	GGNNCC	6 cut(s)	130, 619, 620, 822, 1249, 1483
NmeAIII	GCCGAG	2 cut(s)	157, 457
NmuCI	GTSAC	2 cut(s)	690, 1302
NsiI	ATGCAT	1 cut(s)	1082
NspI	RCATGY	2 cut(s)	234, 1415
PciI	ACATGT	1 cut(s)	230
PcsI	WCGNNNNNNNCGW	1 cut(s)	26
PctI	GAATGC	1 cut(s)	1082
PdmI	GAANNNNTTC	1 cut(s)	1350
PfeI	GAWTC	3 cut(s)	163, 1057, 1181
PfoI	TCCNGGA	1 cut(s)	144
PkrI	GCNGC	5 cut(s)	27, 181, 1095, 1270, 1450
PleI	GAGTC	2 cut(s)	124, 612
PmeI	GTTTAAAC	1 cut(s)	774
PpsI	GAGTC	2 cut(s)	124, 612
PscI	ACATGT	1 cut(s)	230
PshAI	GACNNNNGTC	1 cut(s)	200
PshBI	ATTAAT	2 cut(s)	659, 1146
PsiI	TTATAA	2 cut(s)	581, 720
Psp124BI	GAGCTC	1 cut(s)	1042
Psp6I	CCWGG	2 cut(s)	144, 587
PspGI	CCWGG	2 cut(s)	144, 587
PspN4I	GGNNCC	6 cut(s)	130, 619, 620, 822, 1249, 1483
PspOMI	GGGCCC	1 cut(s)	618
PspPI	GGNCC	9 cut(s)	4, 123, 181, 618, 619, 701, 779, 1247, 1482
PsuI	RGATCY	2 cut(s)	955, 1262
RsaI	GTAC	5 cut(s)	417, 508, 1339, 1418, 1493
RsaNI	GTAC	5 cut(s)	416, 507, 1338, 1417, 1492
RseI	CAYNNNNRTG	2 cut(s)	1158, 1425
SacI	GAGCTC	1 cut(s)	1042
SaqAI	TTAA	8 cut(s)	438, 659, 737, 773, 945, 1029, 1146, 1529
SatI	GCNGC	5 cut(s)	26, 180, 1094, 1269, 1449
Sau3AI	GATC	6 cut(s)	52, 94, 841, 955, 1262, 1429
Sau96I	GGNCC	9 cut(s)	4, 123, 181, 618, 619, 701, 779, 1247, 1482
SchI	GAGTC	2 cut(s)	125, 613
ScrFI	CCNGG	5 cut(s)	146, 589, 1071, 1259, 1260
SduI	GDGCHC	4 cut(s)	622, 1042, 1155, 1411
SfaNI	GCATC	2 cut(s)	418, 949
SinI	GGWCC	3 cut(s)	779, 1247, 1482
SmaI	CCCGGG	1 cut(s)	1260
SmiMI	CAYNNNNRTG	2 cut(s)	1158, 1425
SmlI	CTYRAG	2 cut(s)	113, 736
SmoI	CTYRAG	2 cut(s)	113, 736
Sse9I	AATT	8 cut(s)	363, 535, 722, 743, 860, 871, 1372, 1516
SsiI	CCGC	5 cut(s)	7, 25, 46, 179, 565
SspI	AATATT	2 cut(s)	331, 657
SstI	GAGCTC	1 cut(s)	1042
StyD4I	CCNGG	5 cut(s)	144, 587, 1069, 1257, 1258
StyI	CCWWGG	2 cut(s)	979, 1328
TaaI	ACNGT	3 cut(s)	17, 1223, 1523
TaiI	ACGT	4 cut(s)	240, 284, 1343, 1493
TaqI	TCGA	3 cut(s)	55, 1012, 1218
TasI	AATT	8 cut(s)	363, 535, 722, 743, 860, 871, 1372, 1516
TatI	WGTACW	1 cut(s)	415
TauI	GCSGC	2 cut(s)	28, 182
TfiI	GAWTC	3 cut(s)	163, 1057, 1181
Tru1I	TTAA	8 cut(s)	438, 659, 737, 773, 945, 1029, 1146, 1529
Tru9I	TTAA	8 cut(s)	438, 659, 737, 773, 945, 1029, 1146, 1529
TscAI	CASTG	4 cut(s)	20, 328, 699, 1027
TseFI	GTSAC	2 cut(s)	690, 1302
TseI	GCWGC	3 cut(s)	1093, 1268, 1448
Tsp45I	GTSAC	2 cut(s)	690, 1302
TspDTI	ATGAA	4 cut(s)	383, 392, 1053, 1247
TspGWI	ACGGA	5 cut(s)	212, 387, 792, 1432, 1494
TspMI	CCCGGG	1 cut(s)	1258
TspRI	CASTG	4 cut(s)	20, 328, 699, 1027
Vha464I	CTTAAG	1 cut(s)	736
VneI	GTGCAC	1 cut(s)	1151
VpaK11BI	GGWCC	3 cut(s)	779, 1247, 1482
VspI	ATTAAT	2 cut(s)	659, 1146
XapI	RAATTY	3 cut(s)	722, 743, 860
XceI	RCATGY	2 cut(s)	234, 1415
XmaI	CCCGGG	1 cut(s)	1258
XmnI	GAANNNNTTC	1 cut(s)	1350
Zsp2I	ATGCAT	1 cut(s)	1082

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.