Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0001135

Physical Location & Seq

Genomic Coordinates Forward (+)

26927936 .. 26931495

Gene Structure 3560 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1165200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1455 bp

ATGGCAATTGATATTTCCTTCAATCAGACGGCCGGCCTCAATACACTGTGCTTTCTGGAGCAGGTTGACCAACTGATCGGAAAAGACCATCTTTACAAAAAGAGTATTATCTTAACCAAAGCTTGGTGCTACTATGAGAGTCGAATTCTTGGGGCCCAATATGGCCTGATTGCATCATATGGACTGGAAACATTGGTGTTATTTATCATCAATCGCTTTCATTCATCGCTCCGTGGTCCGTTAGAGGTCCTGCATAGGTTTTTGGAATACTATAGCACATTTGATTGGGGCAACTATGGTATCAGTATAAATGGTCCATTTGCCTTATCTTCCCTTCCTGAAATCATAGTGACACCCCAAAACGAGGGGGAAGAATTTCTCCTGAGCGAGGAGTTTGTAAGAGAGTGCAGGCTGGTGTTTCCAGATCCAGCTGTGGCAAACGCAGCTAGAGTTGATGAATTCCAAATTAAGTTCCTCAACATAGTGGATCCTCTAAAGGACAACAACAATCTAGGCTGTAGCGTCAGTAAAGGAAACTTCTGTCGTATAAGATCTGCTTTTTCCCTTGGGGTTAAAAAATTTCGAGAAGTATTAATGCTACCAGGTGAACGCAGAGGCATGGCACTTGACAAATTCTTCGCGACCACTCTAGATAGGAATGGGGGTGGACAGAGAGCAGATCTGGCAACTCCTGTCCCTGGATTTGGTACTGGAACATCTGAAAAATCTGACCTCACTGGGGACTACGAAGAATATTGTGATAGCCTGTTGTCTGCAAATGCATATCAGTTTTCTATTACACACAACAGTGCTCCACCTTTTGAAGCCGATGACATGAGCAAATGGAATGCCCTGGTTCATACGGCTCAACAAGATGGGAATGTCATTTTTCAAATGGCAGAAAATGCAGACCAAATCTTTTCATGTCGCCTTCCTCACCGGGTAATGCAGAATCAAAATAATTCCACCAATGTGCCTCCAAGAGCGAGGGGAAAAGTCCCGGAGTCTAGCAGCTGCGGTCTGAGGATGAAATCACTTCAAAAGACTAACCAAGTTGAGGTTTCCATCAAAGCAGGCCCGAGTGAAAATGCTCCTTGGTTTAAATTTTTACCTGAAGAACAAAATGATTCCACCAATGTGTCTCCAAGAGCGAGGGGAAAGATCCCGGAGTCTAGCAGCCGTTGTCCAAGGATGAAATTGCCTCAAAAGACTAACCAAGTTGAGGTTTCCACCGAAGCAGGCCCAAGTGAAAATGCTCCCCAGTTTAAATTTTCACCTGAAGAATTCCCACAGCTTCCTGGCACTCAAATGCCTTCATCATCAGATGTACATCAATCTGCTCAGACTGAATTGGCATCTCCTCCAGCCAAGGAATTCCCACAGGTTACCGCAGCGACGATTTGCTTTAAGAGGTTCAAGCTAGACAATGATAATGACTTCCCCCCCTTGACCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NTP_transf_2 PAP_assoc

Protein Analysis

485

Amino Acids

53.92

Weight (kDa)

5.57

Isoelectric Point (pI)

41.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PAP-OAS1	PF26180	26 - 217	8.1e-82	PAP/OAS1 substrate-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001135)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40520 AT2G40520 AT2G40520 AT2G40520 AT2G40520 AT2G40520 AT3G56320 AT3G56320 AT3G56320 AT3G56320 AT3G56320 AT3G56320 AT3G56320
fragaria_vesca	FvH4_3g40330 FvH4_3g40330 FvH4_3g40330 FvH4_7g25750 FvH4_7g25750 FvH4_7g25750 FvH4_7g25750
malus_domestica	MD01G1165200.v1.1 MD03G1053200.v1.1 MD07G1232500.v1.1 MD11G1054900.v1.1
prunus_persica	Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.6G042500_v2.0.a1 Prupe.6G042500_v2.0.a1
pyrus_communis	pycom01g17910 pycom03g04230 pycom11g04480
rosa_chinensis	RchiOBHm_Chr1g0372011 RchiOBHm_Chr5g0072681
rosa_laevigata	RLG00000026928 RLG00000036325
rosa_multiflora	Rmu_sc0001755.1_g000036 Rmu_sc0003160.1_g000034
rosa_roxburghii	Rroxscaffold_1G00008460
rosa_rugosa	Rorug01G0367900 Rorug01G0368000 Rorug01G0368100 Rorug01G0368200 Rorug01G0368300 Rorug05G0419200 Rorug05G0419300
rosa_samantha	Rh1AG377600 Rh1BG341000 Rh1CG354200 Rh1DG372000 Rh5AG476200 Rh5BG496400 Rh5CG520200 Rh5DG508800
rosa_wichuraiana	Rw1G033220 Rw5G044220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	52
AccB7I	CCANNNNNTGG	1 cut(s)	123
AccII	CGCG	1 cut(s)	641
AciI	CCGC	2 cut(s)	1017, 1389
AclWI	GGATC	4 cut(s)	419, 482, 495, 1156
AcoI	YGGCCR	1 cut(s)	30
AcsI	RAATTY	9 cut(s)	144, 374, 458, 578, 632, 1103, 1268, 1283, 1373
AcuI	CTGAAG	2 cut(s)	1134, 1299
AfaI	GTAC	2 cut(s)	709, 1329
AfiI	CCNNNNNNNGG	8 cut(s)	123, 364, 388, 698, 704, 739, 1057, 1222
AgsI	TTSAA	5 cut(s)	22, 824, 893, 1040, 1417
AjnI	CCWGG	4 cut(s)	601, 697, 852, 1297
AleI	CACNNNNGTG	3 cut(s)	807, 971, 1136
AluBI	AGCT	6 cut(s)	122, 431, 446, 1014, 1294, 1420
AluI	AGCT	6 cut(s)	122, 431, 446, 1014, 1294, 1420
Alw21I	GWGCWC	1 cut(s)	814
Alw26I	GTCTC	1 cut(s)	1146
AlwI	GGATC	4 cut(s)	419, 482, 495, 1156
Ama87I	CYCGRG	1 cut(s)	1078
AoxI	GGCC	6 cut(s)	30, 34, 153, 163, 1075, 1240
ApaI	GGGCCC	1 cut(s)	157
ApeKI	GCWGC	5 cut(s)	443, 1011, 1014, 1176, 1391
ApoI	RAATTY	9 cut(s)	144, 374, 458, 578, 632, 1103, 1268, 1283, 1373
ArsI	GACNNNNNNTTYG	2 cut(s)	620, 652
AseI	ATTAAT	1 cut(s)	593
Asp700I	GAANNNNTTC	1 cut(s)	375
AspS9I	GGNCC	7 cut(s)	153, 154, 236, 247, 314, 1076, 1241
AsuC2I	CCSGG	3 cut(s)	941, 1001, 1166
AsuHPI	GGTGA	3 cut(s)	617, 929, 1266
AvaI	CYCGRG	1 cut(s)	1078
AvaII	GGWCC	3 cut(s)	236, 247, 314
BaeGI	GKGCMC	1 cut(s)	157
BaeI	ACNNNNGTAYC	2 cut(s)	283, 316
BamHI	GGATCC	1 cut(s)	487
BanII	GRGCYC	1 cut(s)	157
Bbv12I	GWGCWC	1 cut(s)	814
BbvI	GCAGC	5 cut(s)	455, 1001, 1023, 1188, 1403
BccI	CCATC	3 cut(s)	96, 869, 1073
BceAI	ACGGC	3 cut(s)	45, 879, 1164
BciT130I	CCWGG	4 cut(s)	603, 699, 854, 1299
BcnI	CCSGG	3 cut(s)	941, 1001, 1166
BcoDI	GTCTC	1 cut(s)	1146
BfaI	CTAG	6 cut(s)	447, 512, 650, 1008, 1173, 1421
BfmI	CTRYAG	2 cut(s)	271, 517
BfuAI	ACCTGC	1 cut(s)	52
BglII	AGATCT	2 cut(s)	551, 679
BisI	GCNGC	5 cut(s)	444, 1012, 1015, 1177, 1392
BlsI	GCNGC	5 cut(s)	445, 1013, 1016, 1178, 1393
Bme1390I	CCNGG	7 cut(s)	603, 699, 854, 941, 1001, 1166, 1299
Bme18I	GGWCC	3 cut(s)	236, 247, 314
BmeT110I	CYCGRG	1 cut(s)	1078
BmgT120I	GGNCC	7 cut(s)	153, 154, 236, 247, 314, 1076, 1241
BmiI	GGNNCC	3 cut(s)	154, 155, 489
BmrFI	CCNGG	7 cut(s)	603, 699, 854, 941, 1001, 1166, 1299
BmrI	ACTGGG	2 cut(s)	747, 1255
BmsI	GCATC	2 cut(s)	182, 1364
BmuI	ACTGGG	2 cut(s)	747, 1255
BpmI	CTGGAG	2 cut(s)	77, 1347
Bpu10I	CCTNAGC	1 cut(s)	383
BpuMI	CCSGG	3 cut(s)	941, 1001, 1166
BsaBI	GATNNNNATC	1 cut(s)	1329
BsaJI	CCNNGG	7 cut(s)	232, 565, 697, 852, 1094, 1187, 1368
Bsc4I	CCNNNNNNNGG	8 cut(s)	123, 364, 388, 698, 704, 739, 1057, 1222
Bse118I	RCCGGY	1 cut(s)	32
Bse1I	ACTGG	4 cut(s)	189, 715, 742, 1261
Bse8I	GATNNNNATC	1 cut(s)	1329
BseBI	CCWGG	4 cut(s)	603, 699, 854, 1299
BseDI	CCNNGG	7 cut(s)	232, 565, 697, 852, 1094, 1187, 1368
BseGI	GGATG	2 cut(s)	1032, 1197
BseJI	GATNNNNATC	1 cut(s)	1329
BseLI	CCNNNNNNNGG	8 cut(s)	123, 364, 388, 698, 704, 739, 1057, 1222
BseMII	CTCAG	3 cut(s)	374, 1013, 1355
BseNI	ACTGG	4 cut(s)	189, 715, 742, 1261
BseRI	GAGGAG	2 cut(s)	404, 1350
BseSI	GKGCMC	1 cut(s)	157
BseX3I	CGGCCG	1 cut(s)	30
BseXI	GCAGC	5 cut(s)	455, 1001, 1023, 1188, 1403
BsgI	GTGCAG	1 cut(s)	427
Bsh1236I	CGCG	1 cut(s)	641
Bsh1285I	CGRYCG	1 cut(s)	33
BshFI	GGCC	6 cut(s)	32, 36, 155, 165, 1077, 1242
BsiEI	CGRYCG	1 cut(s)	33
BsiHKAI	GWGCWC	1 cut(s)	814
BsiHKCI	CYCGRG	1 cut(s)	1078
BsiSI	CCGG	4 cut(s)	33, 940, 1001, 1166
BslFI	GGGAC	3 cut(s)	680, 755, 983
BslI	CCNNNNNNNGG	8 cut(s)	123, 364, 388, 698, 704, 739, 1057, 1222
BsmAI	GTCTC	1 cut(s)	1146
BsmFI	GGGAC	3 cut(s)	680, 755, 983
BsmI	GAATGC	1 cut(s)	853
BsnI	GGCC	6 cut(s)	32, 36, 155, 165, 1077, 1242
BsoBI	CYCGRG	1 cut(s)	1078
Bsp120I	GGGCCC	1 cut(s)	153
Bsp1286I	GDGCHC	2 cut(s)	157, 814
Bsp1407I	TGTACA	1 cut(s)	1327
Bsp143I	GATC	6 cut(s)	75, 424, 487, 551, 679, 1161
Bsp68I	TCGCGA	1 cut(s)	641
BspACI	CCGC	2 cut(s)	1017, 1389
BspANI	GGCC	6 cut(s)	32, 36, 155, 165, 1077, 1242
BspCNI	CTCAG	3 cut(s)	375, 1014, 1354
BspFNI	CGCG	1 cut(s)	641
BspLI	GGNNCC	3 cut(s)	154, 155, 489
BspMI	ACCTGC	1 cut(s)	52
BspPI	GGATC	4 cut(s)	419, 482, 495, 1156
BsrFI	RCCGGY	1 cut(s)	32
BsrGI	TGTACA	1 cut(s)	1327
BsrI	ACTGG	4 cut(s)	189, 715, 742, 1261
BssAI	RCCGGY	1 cut(s)	32
BssECI	CCNNGG	7 cut(s)	232, 565, 697, 852, 1094, 1187, 1368
BssMI	GATC	6 cut(s)	75, 424, 487, 551, 679, 1161
BssT1I	CCWWGG	4 cut(s)	565, 1094, 1187, 1368
Bst2UI	CCWGG	4 cut(s)	603, 699, 854, 1299
Bst4CI	ACNGT	2 cut(s)	48, 809
BstAPI	GCANNNNNTGC	1 cut(s)	905
BstAUI	TGTACA	1 cut(s)	1327
BstC8I	GCNNGC	4 cut(s)	34, 410, 1075, 1240
BstDEI	CTNAG	3 cut(s)	383, 1022, 1341
BstDSI	CCRYGG	1 cut(s)	232
BstEII	GGTNACC	1 cut(s)	1384
BstENI	CCTNNNNNAGG	1 cut(s)	386
BstF5I	GGATG	2 cut(s)	1032, 1197
BstFNI	CGCG	1 cut(s)	641
BstKTI	GATC	6 cut(s)	78, 427, 490, 554, 682, 1164
BstMAI	GTCTC	1 cut(s)	1146
BstMBI	GATC	6 cut(s)	75, 424, 487, 551, 679, 1161
BstMCI	CGRYCG	1 cut(s)	33
BstMWI	GCNNNNNNNGC	3 cut(s)	443, 683, 905
BstNI	CCWGG	4 cut(s)	603, 699, 854, 1299
BstPI	GGTNACC	1 cut(s)	1384
BstSCI	CCNGG	7 cut(s)	601, 697, 852, 939, 999, 1164, 1297
BstSFI	CTRYAG	2 cut(s)	271, 517
BstSLI	GKGCMC	1 cut(s)	157
BstUI	CGCG	1 cut(s)	641
BstV1I	GCAGC	5 cut(s)	455, 1001, 1023, 1188, 1403
BstX2I	RGATCY	5 cut(s)	424, 487, 551, 679, 1161
BstYI	RGATCY	5 cut(s)	424, 487, 551, 679, 1161
BstZI	CGGCCG	1 cut(s)	30
BsuRI	GGCC	6 cut(s)	32, 36, 155, 165, 1077, 1242
BtgI	CCRYGG	1 cut(s)	232
BtgZI	GCGATG	1 cut(s)	210
BtsCI	GGATG	2 cut(s)	1032, 1197
BtsIMutI	CAGTG	3 cut(s)	44, 735, 814
BtuMI	TCGCGA	1 cut(s)	641
BveI	ACCTGC	1 cut(s)	52
Cac8I	GCNNGC	4 cut(s)	34, 410, 1075, 1240
Cfr10I	RCCGGY	1 cut(s)	32
Cfr13I	GGNCC	7 cut(s)	153, 154, 236, 247, 314, 1076, 1241
CseI	GACGC	1 cut(s)	511
CsiI	ACCWGGT	1 cut(s)	601
Csp6I	GTAC	2 cut(s)	708, 1328
CviAII	CATG	3 cut(s)	619, 835, 924
CviQI	GTAC	2 cut(s)	708, 1328
DdeI	CTNAG	3 cut(s)	383, 1022, 1341
DpnI	GATC	6 cut(s)	77, 426, 489, 553, 681, 1163
DpnII	GATC	6 cut(s)	75, 424, 487, 551, 679, 1161
DraI	TTTAAA	2 cut(s)	1102, 1267
EaeI	YGGCCR	1 cut(s)	30
EagI	CGGCCG	1 cut(s)	30
EclXI	CGGCCG	1 cut(s)	30
Eco130I	CCWWGG	4 cut(s)	565, 1094, 1187, 1368
Eco24I	GRGCYC	1 cut(s)	157
Eco47I	GGWCC	3 cut(s)	236, 247, 314
Eco52I	CGGCCG	1 cut(s)	30
Eco57I	CTGAAG	2 cut(s)	1134, 1299
Eco88I	CYCGRG	1 cut(s)	1078
Eco91I	GGTNACC	1 cut(s)	1384
EcoNI	CCTNNNNNAGG	1 cut(s)	386
EcoO109I	RGGNCCY	2 cut(s)	153, 247
EcoO65I	GGTNACC	1 cut(s)	1384
EcoRI	GAATTC	4 cut(s)	144, 458, 1283, 1373
EcoRII	CCWGG	4 cut(s)	601, 697, 852, 1297
EcoT14I	CCWWGG	4 cut(s)	565, 1094, 1187, 1368
EcoT22I	ATGCAT	1 cut(s)	784
EcoT38I	GRGCYC	1 cut(s)	157
ErhI	CCWWGG	4 cut(s)	565, 1094, 1187, 1368
FaeI	CATG	3 cut(s)	622, 838, 927
FalI	AAGNNNNNCTT	4 cut(s)	75, 107, 541, 573
FaqI	GGGAC	3 cut(s)	680, 755, 983
FatI	CATG	3 cut(s)	618, 834, 923
FauNDI	CATATG	1 cut(s)	178
Fnu4HI	GCNGC	5 cut(s)	444, 1012, 1015, 1177, 1392
FokI	GGATG	2 cut(s)	1039, 1204
FriOI	GRGCYC	1 cut(s)	157
FseI	GGCCGGCC	1 cut(s)	36
Fsp4HI	GCNGC	5 cut(s)	444, 1012, 1015, 1177, 1392
FspBI	CTAG	6 cut(s)	447, 512, 650, 1008, 1173, 1421
GluI	GCNGC	5 cut(s)	444, 1012, 1015, 1177, 1392
GsuI	CTGGAG	2 cut(s)	77, 1347
HaeIII	GGCC	6 cut(s)	32, 36, 155, 165, 1077, 1242
HapII	CCGG	4 cut(s)	33, 940, 1001, 1166
HgaI	GACGC	1 cut(s)	511
Hin1II	CATG	3 cut(s)	622, 838, 927
HincII	GTYRAC	1 cut(s)	67
HindII	GTYRAC	1 cut(s)	67
HindIII	AAGCTT	1 cut(s)	120
HinfI	GANTC	5 cut(s)	139, 952, 1004, 1127, 1169
HpaII	CCGG	4 cut(s)	33, 940, 1001, 1166
HphI	GGTGA	3 cut(s)	617, 929, 1266
Hpy166II	GTNNAC	3 cut(s)	67, 608, 668
Hpy188I	TCNGA	7 cut(s)	27, 80, 721, 730, 1023, 1324, 1344
Hpy188III	TCNNGA	7 cut(s)	56, 338, 382, 422, 584, 640, 650
Hpy8I	GTNNAC	3 cut(s)	67, 608, 668
Hpy99I	CGWCG	1 cut(s)	1399
HpyAV	CCTTC	4 cut(s)	28, 344, 941, 1323
HpyCH4III	ACNGT	2 cut(s)	48, 809
HpyCH4V	TGCA	7 cut(s)	173, 253, 408, 776, 782, 908, 949
HpyF10VI	GCNNNNNNNGC	3 cut(s)	443, 683, 905
HpyF3I	CTNAG	3 cut(s)	383, 1022, 1341
Hsp92II	CATG	3 cut(s)	622, 838, 927
KroI	GCCGGC	1 cut(s)	32
KroNI	GCCGGC	1 cut(s)	34
Kzo9I	GATC	6 cut(s)	75, 424, 487, 551, 679, 1161
LmnI	GCTCC	5 cut(s)	58, 234, 817, 1096, 1261
Lsp1109I	GCAGC	5 cut(s)	455, 1001, 1023, 1188, 1403
LweI	GCATC	2 cut(s)	182, 1364
MabI	ACCWGGT	1 cut(s)	601
MaeI	CTAG	6 cut(s)	447, 512, 650, 1008, 1173, 1421
MaeIII	GTNAC	2 cut(s)	349, 1384
MalI	GATC	6 cut(s)	77, 426, 489, 553, 681, 1163
MboI	GATC	6 cut(s)	75, 424, 487, 551, 679, 1161
MboII	GAAGA	6 cut(s)	321, 383, 628, 761, 1127, 1292
MfeI	CAATTG	1 cut(s)	6
MflI	RGATCY	5 cut(s)	424, 487, 551, 679, 1161
MhlI	GDGCHC	2 cut(s)	157, 814
MlyI	GAGTC	3 cut(s)	148, 1013, 1178
Mph1103I	ATGCAT	1 cut(s)	784
MroNI	GCCGGC	1 cut(s)	32
MroXI	GAANNNNTTC	1 cut(s)	375
MseI	TTAA	7 cut(s)	113, 468, 573, 593, 1101, 1266, 1407
MslI	CAYNNNNRTG	4 cut(s)	807, 971, 1136, 1307
MspA1I	CMGCKG	2 cut(s)	431, 1014
MspI	CCGG	4 cut(s)	33, 940, 1001, 1166
MspR9I	CCNGG	7 cut(s)	603, 699, 854, 941, 1001, 1166, 1299
MunI	CAATTG	1 cut(s)	6
Mva1269I	GAATGC	1 cut(s)	853
MvaI	CCWGG	4 cut(s)	603, 699, 854, 1299
MvnI	CGCG	1 cut(s)	641
MwoI	GCNNNNNNNGC	3 cut(s)	443, 683, 905
NaeI	GCCGGC	1 cut(s)	34
NciI	CCSGG	3 cut(s)	941, 1001, 1166
NdeI	CATATG	1 cut(s)	178
NdeII	GATC	6 cut(s)	75, 424, 487, 551, 679, 1161
NgoMIV	GCCGGC	1 cut(s)	32
NlaIII	CATG	3 cut(s)	622, 838, 927
NlaIV	GGNNCC	3 cut(s)	154, 155, 489
NmuCI	GTSAC	1 cut(s)	349
NruI	TCGCGA	1 cut(s)	641
NsiI	ATGCAT	1 cut(s)	784
OliI	CACNNNNGTG	3 cut(s)	807, 971, 1136
PctI	GAATGC	1 cut(s)	853
PdiI	GCCGGC	1 cut(s)	34
PdmI	GAANNNNTTC	1 cut(s)	375
PfeI	GAWTC	2 cut(s)	952, 1127
PflMI	CCANNNNNTGG	1 cut(s)	123
PfoI	TCCNGGA	2 cut(s)	999, 1164
PkrI	GCNGC	5 cut(s)	445, 1013, 1016, 1178, 1393
PleI	GAGTC	3 cut(s)	147, 1012, 1177
PpsI	GAGTC	3 cut(s)	147, 1012, 1177
PpuMI	RGGWCCY	1 cut(s)	247
PshBI	ATTAAT	1 cut(s)	593
Psp5II	RGGWCCY	1 cut(s)	247
Psp6I	CCWGG	4 cut(s)	601, 697, 852, 1297
PspEI	GGTNACC	1 cut(s)	1384
PspGI	CCWGG	4 cut(s)	601, 697, 852, 1297
PspN4I	GGNNCC	3 cut(s)	154, 155, 489
PspOMI	GGGCCC	1 cut(s)	153
PspPI	GGNCC	7 cut(s)	153, 154, 236, 247, 314, 1076, 1241
PspPPI	RGGWCCY	1 cut(s)	247
PsuI	RGATCY	5 cut(s)	424, 487, 551, 679, 1161
PvuII	CAGCTG	2 cut(s)	431, 1014
RigI	GGCCGGCC	1 cut(s)	36
RruI	TCGCGA	1 cut(s)	641
RsaI	GTAC	2 cut(s)	709, 1329
RsaNI	GTAC	2 cut(s)	708, 1328
RseI	CAYNNNNRTG	4 cut(s)	807, 971, 1136, 1307
SaqAI	TTAA	7 cut(s)	113, 468, 573, 593, 1101, 1266, 1407
SatI	GCNGC	5 cut(s)	444, 1012, 1015, 1177, 1392
Sau3AI	GATC	6 cut(s)	75, 424, 487, 551, 679, 1161
Sau96I	GGNCC	7 cut(s)	153, 154, 236, 247, 314, 1076, 1241
SchI	GAGTC	3 cut(s)	148, 1013, 1178
ScrFI	CCNGG	7 cut(s)	603, 699, 854, 941, 1001, 1166, 1299
SduI	GDGCHC	2 cut(s)	157, 814
SexAI	ACCWGGT	1 cut(s)	601
SfaNI	GCATC	2 cut(s)	182, 1364
SfcI	CTRYAG	2 cut(s)	271, 517
SinI	GGWCC	3 cut(s)	236, 247, 314
SmiMI	CAYNNNNRTG	4 cut(s)	807, 971, 1136, 1307
SsiI	CCGC	2 cut(s)	1017, 1389
SspI	AATATT	1 cut(s)	755
SspMI	CTAG	6 cut(s)	447, 512, 650, 1008, 1173, 1421
StyD4I	CCNGG	7 cut(s)	601, 697, 852, 939, 999, 1164, 1297
StyI	CCWWGG	4 cut(s)	565, 1094, 1187, 1368
TaaI	ACNGT	2 cut(s)	48, 809
TaqI	TCGA	2 cut(s)	142, 583
TatI	WGTACW	1 cut(s)	1327
TfiI	GAWTC	2 cut(s)	952, 1127
Tru1I	TTAA	7 cut(s)	113, 468, 573, 593, 1101, 1266, 1407
Tru9I	TTAA	7 cut(s)	113, 468, 573, 593, 1101, 1266, 1407
TscAI	CASTG	3 cut(s)	51, 742, 814
TseFI	GTSAC	1 cut(s)	349
TseI	GCWGC	5 cut(s)	443, 1011, 1014, 1176, 1391
Tsp45I	GTSAC	1 cut(s)	349
TspDTI	ATGAA	8 cut(s)	209, 213, 471, 848, 912, 1043, 1208, 1305
TspGWI	ACGGA	2 cut(s)	221, 228
TspRI	CASTG	3 cut(s)	51, 742, 814
Van91I	CCANNNNNTGG	1 cut(s)	123
VpaK11BI	GGWCC	3 cut(s)	236, 247, 314
VspI	ATTAAT	1 cut(s)	593
XagI	CCTNNNNNAGG	1 cut(s)	386
XapI	RAATTY	9 cut(s)	144, 374, 458, 578, 632, 1103, 1268, 1283, 1373
XbaI	TCTAGA	1 cut(s)	649
XmnI	GAANNNNTTC	1 cut(s)	375
XspI	CTAG	6 cut(s)	447, 512, 650, 1008, 1173, 1421
Zsp2I	ATGCAT	1 cut(s)	784

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.