MD07G1232500.v1.1

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0001135
Physical Location & Seq
Forward (+)
30636012 .. 30645093
9082 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1232500.v1.1.491

Sequence Viewer

Length: 1878 bp
ATGAAAGATTGGATTCTTGCGTTACAGGTCTTCTCCTTTGGCTCAGTACCATTGAAGACCTATCTTCCTGATGGAGATATTGATTTGACTGCTCTCTGCCTTCCAAACTTGGTAGATATGCTTGCTCTAAAAATCTGTGCGATCCTCGAGGATCACCAACCACAGGACTCCAAGTTCCAAATTAAAGACGTCTGCTACGTCCGCGCACAGGTTAAGGTAGTAAAATGCATTGTGAATAACATGGCAATCGACATCTCCTTCAACCAGACTGCCGGTCTCAGCACACTGTGCTTTCTGGAGCAGGTTGATCGACTGTTCGGAAAAGACCATCTTTTCAAAAAGAGTATTATCTTAATCAAAGCTTGGTGCTACTATGAGAGTCGAATTCTTGGGGCCCATTATGGCCTGATTGCAGCATATGGACTGGAAACGTTGGTGTTGTTTATCGTCAATCGCTTTCATTCATCGCTCCATGGTCCTTTAGAGGTCCTGCTTATATTTTTGGAATTCTATAGCACATTTGATTGGGAAAATTATGCTCTCAGTATAAATGGTCCAGTTGCCTTAGCTTCCCTTCCTGAAATCATAGTGACACCCCAAAACGAGGGGGAAGAATTTCTCCTGAGTGAGGAGTTTGTAAGAGAGTGCAGGCTGGCGTTTCCAGATCCAGCAGCGGCAGAGGCAGATAGTGTTGATGAATTCCCAATTAAGTTCCTCAACATAGTGGATCCTCTAAAGGACAACAACAATCTAGGCCGTAGTGTTAGTAAAGGAAATTTCTATCGTATAAGATCTGCTTTTTCCCTTGGGGCTCAAACACTTAGAGAGGTATTAATGCTACCGGGTGAACGCATAGGCATGGCACTTGAGAACTTCTTTGTGACCACTCTAGATAGGAATGGGCGTGGAGAAAGGGCAGATCTGACAACTCCTGTCCCTGCATTTGGTACTGGAACATCTGTAGAATCTGACCTTACTGGGGACTACGACAAACATTGTGATAGCATGTTGTTTGCAAATGCATATCAGTTTTTTAATACACACAACGGTGCTCCACCTTTTGTAGACGATGATATGAGCAAATGGAATTCCCTGGTTTATATGGCTCAACTATATGGGAATGTCATTTTTCAAATGGCAGAAAACGCATACCAATCTGCTGCTACTTTTGGTGTTGGGGTGATAAAGAAATCACACGGAACAGGCACATATATACCTAACCTGGCACTGCAAAATCAAAATGATTCCACAAATGTGCCTCCAAGAGCGAGGAGAATGATTCCGGAGTCTAGCAATTGCGGTCTGAGGATGAAATCACCTCGAAAGACAAACCAAGTTGAGGTTTTCACAGAAGCAGGCCCGAGTGAAAATGTTCACCAGTTTAATTTTTCACCTGAAGAATTCCCACAGCTTCCCGGCATTCAAATACCCTCACTGTCAGAAGTACATCAATCTGCTCAGCCTGAATTGGCGTCTCCTCCAGCCAAAGAACTGAGGATGAAATCACCTCGAAAGACGAACCAAGTTGAGGTTTCCACAGAAGTAGGCCCGAGTGAAAATGCTCACCAGTTTAATTTTTCACCTGAAGAATTCCCACTGCTTCCCGGCACTCAACTACCCTCATTGTCAGAAGTACACCAATCTGCTCAGCCCGAATTGGCGTCTCCTCCAGCCAAAGAATCCTCTCACACTTCCGGGAAGTCTAAGTTGGGGAATTCTGAGCATTCACTGTCACTACCGGAAATGCCTTCACCTGTAGCAAGCCCACAGGTAAATACTAGTGCTTCATATGCTCAGAACTTTATACCTGGGGTCCCTGCGATGGGAATGCAGAAGCAACAAGAACTGTCAGAGTTAACGAAGCGACGGTTTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2 PAP_assoc

Protein Analysis

626

Amino Acids

69.26

Weight (kDa)

5.26

Isoelectric Point (pI)

44.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PAP-OAS1 PF26180 107 - 297 6.6e-85 PAP/OAS1 substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 192
AbsI CCTCGAGG 1 cut(s) 146
Acc36I ACCTGC 1 cut(s) 292
AccI GTMKAC 1 cut(s) 1065
AccII CGCG 1 cut(s) 204
AccIII TCCGGA 1 cut(s) 1282
AciI CCGC 3 cut(s) 202, 674, 1299
AclI AACGTT 1 cut(s) 431
AclWI GGATC 5 cut(s) 136, 159, 659, 722, 735
AcsI RAATTY 9 cut(s) 384, 506, 614, 698, 775, 1087, 1400, 1589, 1714
AcuI CTGAAG 2 cut(s) 1416, 1605
AcyI GRCGYC 3 cut(s) 189, 1472, 1661
AdeI CACNNNGTG 1 cut(s) 288
AfaI GTAC 4 cut(s) 48, 949, 1446, 1635
AgsI TTSAA 5 cut(s) 55, 262, 337, 1133, 1424
AhdI GACNNNNNGTC 1 cut(s) 273
AhlI ACTAGT 1 cut(s) 1778
AjnI CCWGG 3 cut(s) 1092, 1221, 1807
AjuI GAANNNNNNNTTGG 2 cut(s) 1691, 1723
AleI CACNNNNGTG 2 cut(s) 1047, 1253
AloI GAACNNNNNNTCC 2 cut(s) 158, 190
AluBI AGCT 3 cut(s) 362, 569, 1411
AluI AGCT 3 cut(s) 362, 569, 1411
Alw21I GWGCWC 1 cut(s) 1054
Alw26I GTCTC 3 cut(s) 281, 1479, 1668
AlwI GGATC 5 cut(s) 136, 159, 659, 722, 735
Ama87I CYCGRG 3 cut(s) 146, 1360, 1549
Aor13HI TCCGGA 1 cut(s) 1282
AoxI GGCC 5 cut(s) 393, 403, 754, 1357, 1546
ApaI GGGCCC 1 cut(s) 397
ApeKI GCWGC 3 cut(s) 413, 671, 1160
ApoI RAATTY 9 cut(s) 384, 506, 614, 698, 775, 1087, 1400, 1589, 1714
AseI ATTAAT 1 cut(s) 833
Asp700I GAANNNNTTC 2 cut(s) 615, 1371
AspLEI GCGC 1 cut(s) 206
AspS9I GGNCC 8 cut(s) 393, 394, 476, 487, 554, 1358, 1547, 1813
AsuC2I CCSGG 4 cut(s) 843, 1416, 1605, 1696
AvaI CYCGRG 3 cut(s) 146, 1360, 1549
AvaII GGWCC 4 cut(s) 476, 487, 554, 1813
BaeGI GKGCMC 1 cut(s) 397
BamHI GGATCC 1 cut(s) 727
BanII GRGCYC 2 cut(s) 397, 814
BbsI GAAGAC 2 cut(s) 22, 62
Bbv12I GWGCWC 1 cut(s) 1054
BbvI GCAGC 3 cut(s) 425, 683, 1147
BccI CCATC 3 cut(s) 65, 336, 1816
BceAI ACGGC 1 cut(s) 741
BcgI CGANNNNNNTGC 4 cut(s) 290, 324, 1810, 1844
BciT130I CCWGG 3 cut(s) 1094, 1223, 1809
BcnI CCSGG 4 cut(s) 843, 1416, 1605, 1696
BcoDI GTCTC 3 cut(s) 281, 1479, 1668
BcuI ACTAGT 1 cut(s) 1778
BfaI CTAG 4 cut(s) 752, 890, 1290, 1779
BfmI CTRYAG 3 cut(s) 511, 960, 1755
BfuAI ACCTGC 1 cut(s) 292
BglII AGATCT 2 cut(s) 791, 919
BisI GCNGC 4 cut(s) 414, 672, 675, 1161
BlpI GCTNAGC 2 cut(s) 1458, 1647
BlsI GCNGC 4 cut(s) 415, 673, 676, 1162
Bme1390I CCNGG 7 cut(s) 843, 1094, 1223, 1416, 1605, 1696, 1809
Bme18I GGWCC 4 cut(s) 476, 487, 554, 1813
BmeRI GACNNNNNGTC 1 cut(s) 273
BmeT110I CYCGRG 3 cut(s) 146, 1360, 1549
BmgT120I GGNCC 8 cut(s) 393, 394, 476, 487, 554, 1358, 1547, 1813
BmiI GGNNCC 5 cut(s) 394, 395, 729, 1814, 1815
BmrFI CCNGG 7 cut(s) 843, 1094, 1223, 1416, 1605, 1696, 1809
BmrI ACTGGG 1 cut(s) 987
BmuI ACTGGG 1 cut(s) 987
BpiI GAAGAC 2 cut(s) 22, 62
BpmI CTGGAG 3 cut(s) 317, 1464, 1653
Bpu10I CCTNAGC 1 cut(s) 565
Bpu1102I GCTNAGC 2 cut(s) 1458, 1647
BpuEI CTTGAG 1 cut(s) 887
BpuMI CCSGG 4 cut(s) 843, 1416, 1605, 1696
BsaHI GRCGYC 3 cut(s) 189, 1472, 1661
BsaI GGTCTC 1 cut(s) 281
BsaJI CCNNGG 4 cut(s) 472, 805, 1092, 1808
BsaWI WCCGGW 2 cut(s) 1282, 1738
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 6 cut(s) 429, 557, 955, 982, 1378, 1567
BseAI TCCGGA 1 cut(s) 1282
BseBI CCWGG 3 cut(s) 1094, 1223, 1809
BseDI CCNNGG 4 cut(s) 472, 805, 1092, 1808
BseGI GGATG 2 cut(s) 1314, 1503
BseNI ACTGG 6 cut(s) 429, 557, 955, 982, 1378, 1567
BseRI GAGGAG 4 cut(s) 644, 1285, 1467, 1656
BseSI GKGCMC 1 cut(s) 397
BseXI GCAGC 3 cut(s) 425, 683, 1147
BsgI GTGCAG 1 cut(s) 667
Bsh1236I CGCG 1 cut(s) 204
BshFI GGCC 5 cut(s) 395, 405, 756, 1359, 1548
BsiHKAI GWGCWC 1 cut(s) 1054
BsiHKCI CYCGRG 3 cut(s) 146, 1360, 1549
BsiSI CCGG 7 cut(s) 273, 842, 1283, 1416, 1605, 1695, 1739
BslFI GGGAC 3 cut(s) 920, 995, 1799
BsmAI GTCTC 3 cut(s) 281, 1479, 1668
BsmBI CGTCTC 2 cut(s) 1479, 1668
BsmFI GGGAC 3 cut(s) 920, 995, 1799
BsmI GAATGC 3 cut(s) 1419, 1723, 1833
BsnI GGCC 5 cut(s) 395, 405, 756, 1359, 1548
Bso31I GGTCTC 1 cut(s) 281
BsoBI CYCGRG 3 cut(s) 146, 1360, 1549
Bsp120I GGGCCC 1 cut(s) 393
Bsp1286I GDGCHC 3 cut(s) 397, 814, 1054
Bsp13I TCCGGA 1 cut(s) 1282
Bsp143I GATC 7 cut(s) 141, 151, 307, 664, 727, 791, 919
Bsp1720I GCTNAGC 2 cut(s) 1458, 1647
Bsp19I CCATGG 1 cut(s) 472
BspACI CCGC 3 cut(s) 202, 674, 1299
BspANI GGCC 5 cut(s) 395, 405, 756, 1359, 1548
BspEI TCCGGA 1 cut(s) 1282
BspFNI CGCG 1 cut(s) 204
BspLI GGNNCC 5 cut(s) 394, 395, 729, 1814, 1815
BspMI ACCTGC 1 cut(s) 292
BspPI GGATC 5 cut(s) 136, 159, 659, 722, 735
BspTNI GGTCTC 1 cut(s) 281
BsrFI RCCGGY 1 cut(s) 272
BsrI ACTGG 6 cut(s) 429, 557, 955, 982, 1378, 1567
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 4 cut(s) 472, 805, 1092, 1808
BssMI GATC 7 cut(s) 141, 151, 307, 664, 727, 791, 919
BssNI GRCGYC 3 cut(s) 189, 1472, 1661
BssT1I CCWWGG 2 cut(s) 472, 805
Bst2UI CCWGG 3 cut(s) 1094, 1223, 1809
Bst4CI ACNGT 7 cut(s) 288, 315, 1049, 1437, 1731, 1848, 1869
BstACI GRCGYC 3 cut(s) 189, 1472, 1661
BstAPI GCANNNNNTGC 1 cut(s) 288
BstC8I GCNNGC 5 cut(s) 123, 650, 654, 1357, 1762
BstDSI CCRYGG 1 cut(s) 472
BstENI CCTNNNNNAGG 1 cut(s) 626
BstF5I GGATG 2 cut(s) 1314, 1503
BstFNI CGCG 1 cut(s) 204
BstHHI GCGC 1 cut(s) 206
BstKTI GATC 7 cut(s) 144, 154, 310, 667, 730, 794, 922
BstMAI GTCTC 3 cut(s) 281, 1479, 1668
BstMBI GATC 7 cut(s) 141, 151, 307, 664, 727, 791, 919
BstMWI GCNNNNNNNGC 5 cut(s) 201, 288, 680, 1145, 1790
BstNI CCWGG 3 cut(s) 1094, 1223, 1809
BstNSI RCATGY 1 cut(s) 1009
BstSCI CCNGG 7 cut(s) 841, 1092, 1221, 1414, 1603, 1694, 1807
BstSFI CTRYAG 3 cut(s) 511, 960, 1755
BstSLI GKGCMC 1 cut(s) 397
BstUI CGCG 1 cut(s) 204
BstV1I GCAGC 3 cut(s) 425, 683, 1147
BstV2I GAAGAC 2 cut(s) 22, 62
BstX2I RGATCY 4 cut(s) 664, 727, 791, 919
BstYI RGATCY 4 cut(s) 664, 727, 791, 919
BsuRI GGCC 5 cut(s) 395, 405, 756, 1359, 1548
BtgI CCRYGG 1 cut(s) 472
BtgZI GCGATG 2 cut(s) 450, 1835
BtsCI GGATG 2 cut(s) 1314, 1503
BtsI GCAGTG 2 cut(s) 1226, 1595
BtsIMutI CAGTG 5 cut(s) 284, 1226, 1433, 1595, 1727
BveI ACCTGC 1 cut(s) 292
Cac8I GCNNGC 5 cut(s) 123, 650, 654, 1357, 1762
CfoI GCGC 1 cut(s) 206
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 8 cut(s) 393, 394, 476, 487, 554, 1358, 1547, 1813
CseI GACGC 2 cut(s) 1461, 1650
Csp6I GTAC 4 cut(s) 47, 948, 1445, 1634
CviAII CATG 4 cut(s) 241, 473, 859, 1006
CviQI GTAC 4 cut(s) 47, 948, 1445, 1634
DpnI GATC 7 cut(s) 143, 153, 309, 666, 729, 793, 921
DpnII GATC 7 cut(s) 141, 151, 307, 664, 727, 791, 919
DraIII CACNNNGTG 1 cut(s) 288
DriI GACNNNNNGTC 1 cut(s) 273
Eam1105I GACNNNNNGTC 1 cut(s) 273
Eco130I CCWWGG 2 cut(s) 472, 805
Eco24I GRGCYC 2 cut(s) 397, 814
Eco31I GGTCTC 1 cut(s) 281
Eco47I GGWCC 4 cut(s) 476, 487, 554, 1813
Eco57I CTGAAG 2 cut(s) 1416, 1605
Eco88I CYCGRG 3 cut(s) 146, 1360, 1549
EcoNI CCTNNNNNAGG 1 cut(s) 626
EcoO109I RGGNCCY 3 cut(s) 393, 487, 1813
EcoRI GAATTC 7 cut(s) 384, 506, 698, 1087, 1400, 1589, 1714
EcoRII CCWGG 3 cut(s) 1092, 1221, 1807
EcoT14I CCWWGG 2 cut(s) 472, 805
EcoT22I ATGCAT 2 cut(s) 230, 1024
EcoT38I GRGCYC 2 cut(s) 397, 814
ErhI CCWWGG 2 cut(s) 472, 805
Esp3I CGTCTC 2 cut(s) 1479, 1668
FaeI CATG 4 cut(s) 244, 476, 862, 1009
FalI AAGNNNNNCTT 4 cut(s) 315, 347, 781, 813
FaqI GGGAC 3 cut(s) 920, 995, 1799
FatI CATG 4 cut(s) 240, 472, 858, 1005
FauNDI CATATG 2 cut(s) 418, 1789
FblI GTMKAC 1 cut(s) 1065
Fnu4HI GCNGC 4 cut(s) 414, 672, 675, 1161
FokI GGATG 2 cut(s) 1321, 1510
FriOI GRGCYC 2 cut(s) 397, 814
Fsp4HI GCNGC 4 cut(s) 414, 672, 675, 1161
FspBI CTAG 4 cut(s) 752, 890, 1290, 1779
GlaI GCGC 1 cut(s) 205
GluI GCNGC 4 cut(s) 414, 672, 675, 1161
GsuI CTGGAG 3 cut(s) 317, 1464, 1653
HaeIII GGCC 5 cut(s) 395, 405, 756, 1359, 1548
HapII CCGG 7 cut(s) 273, 842, 1283, 1416, 1605, 1695, 1739
HgaI GACGC 2 cut(s) 1461, 1650
HhaI GCGC 1 cut(s) 206
Hin1I GRCGYC 3 cut(s) 189, 1472, 1661
Hin1II CATG 4 cut(s) 244, 476, 862, 1009
Hin6I GCGC 1 cut(s) 204
HinP1I GCGC 1 cut(s) 204
HincII GTYRAC 1 cut(s) 1857
HindII GTYRAC 1 cut(s) 1857
HindIII AAGCTT 1 cut(s) 360
HinfI GANTC 8 cut(s) 13, 167, 379, 965, 1244, 1279, 1286, 1679
HpaI GTTAAC 1 cut(s) 1857
HpaII CCGG 7 cut(s) 273, 842, 1283, 1416, 1605, 1695, 1739
Hpy166II GTNNAC 5 cut(s) 848, 1066, 1375, 1636, 1857
Hpy188I TCNGA 9 cut(s) 320, 924, 970, 1305, 1441, 1630, 1720, 1797, 1852
Hpy188III TCNNGA 7 cut(s) 68, 296, 578, 622, 662, 890, 1283
Hpy8I GTNNAC 5 cut(s) 848, 1066, 1375, 1636, 1857
Hpy99I CGWCG 1 cut(s) 1869
HpyAV CCTTC 4 cut(s) 110, 268, 584, 1758
HpyCH4III ACNGT 7 cut(s) 288, 315, 1049, 1437, 1731, 1848, 1869
HpyCH4IV ACGT 3 cut(s) 189, 198, 431
HpyCH4V TGCA 8 cut(s) 228, 413, 648, 941, 1016, 1022, 1231, 1831
HpyF10VI GCNNNNNNNGC 5 cut(s) 201, 288, 680, 1145, 1790
HpySE526I ACGT 3 cut(s) 189, 198, 431
Hsp92I GRCGYC 3 cut(s) 189, 1472, 1661
Hsp92II CATG 4 cut(s) 244, 476, 862, 1009
HspAI GCGC 1 cut(s) 204
KflI GGGWCCC 1 cut(s) 1813
Kpn2I TCCGGA 1 cut(s) 1282
KspAI GTTAAC 1 cut(s) 1857
Kzo9I GATC 7 cut(s) 141, 151, 307, 664, 727, 791, 919
LmnI GCTCC 3 cut(s) 298, 474, 1057
Lsp1109I GCAGC 3 cut(s) 425, 683, 1147
MaeI CTAG 4 cut(s) 752, 890, 1290, 1779
MaeII ACGT 3 cut(s) 189, 198, 431
MaeIII GTNAC 4 cut(s) 21, 589, 880, 1731
MalI GATC 7 cut(s) 143, 153, 309, 666, 729, 793, 921
MboI GATC 7 cut(s) 141, 151, 307, 664, 727, 791, 919
MboII GAAGA 6 cut(s) 22, 56, 67, 623, 1409, 1598
MfeI CAATTG 1 cut(s) 1294
MflI RGATCY 4 cut(s) 664, 727, 791, 919
MhlI GDGCHC 3 cut(s) 397, 814, 1054
MlyI GAGTC 3 cut(s) 161, 388, 1295
Mph1103I ATGCAT 2 cut(s) 230, 1024
MroI TCCGGA 1 cut(s) 1282
MroXI GAANNNNTTC 2 cut(s) 615, 1371
MseI TTAA 9 cut(s) 183, 213, 353, 708, 833, 1035, 1383, 1572, 1856
MslI CAYNNNNRTG 3 cut(s) 857, 1047, 1253
MspA1I CMGCKG 1 cut(s) 674
MspI CCGG 7 cut(s) 273, 842, 1283, 1416, 1605, 1695, 1739
MspR9I CCNGG 7 cut(s) 843, 1094, 1223, 1416, 1605, 1696, 1809
MunI CAATTG 1 cut(s) 1294
Mva1269I GAATGC 3 cut(s) 1419, 1723, 1833
MvaI CCWGG 3 cut(s) 1094, 1223, 1809
MvnI CGCG 1 cut(s) 204
MwoI GCNNNNNNNGC 5 cut(s) 201, 288, 680, 1145, 1790
NciI CCSGG 4 cut(s) 843, 1416, 1605, 1696
NcoI CCATGG 1 cut(s) 472
NdeI CATATG 2 cut(s) 418, 1789
NdeII GATC 7 cut(s) 141, 151, 307, 664, 727, 791, 919
NlaIII CATG 4 cut(s) 244, 476, 862, 1009
NlaIV GGNNCC 5 cut(s) 394, 395, 729, 1814, 1815
NmuCI GTSAC 3 cut(s) 589, 880, 1731
NsiI ATGCAT 2 cut(s) 230, 1024
NspI RCATGY 1 cut(s) 1009
OliI CACNNNNGTG 2 cut(s) 1047, 1253
PaeR7I CTCGAG 1 cut(s) 146
PcsI WCGNNNNNNNCGW 1 cut(s) 195
PctI GAATGC 3 cut(s) 1419, 1723, 1833
PdmI GAANNNNTTC 2 cut(s) 615, 1371
PfeI GAWTC 5 cut(s) 13, 965, 1244, 1279, 1679
PfoI TCCNGGA 1 cut(s) 1694
PkrI GCNGC 4 cut(s) 415, 673, 676, 1162
PleI GAGTC 3 cut(s) 161, 387, 1294
PpsI GAGTC 3 cut(s) 161, 387, 1294
PpuMI RGGWCCY 2 cut(s) 487, 1813
PshBI ATTAAT 1 cut(s) 833
Psp1406I AACGTT 1 cut(s) 431
Psp5II RGGWCCY 2 cut(s) 487, 1813
Psp6I CCWGG 3 cut(s) 1092, 1221, 1807
PspGI CCWGG 3 cut(s) 1092, 1221, 1807
PspN4I GGNNCC 5 cut(s) 394, 395, 729, 1814, 1815
PspOMI GGGCCC 1 cut(s) 393
PspPI GGNCC 8 cut(s) 393, 394, 476, 487, 554, 1358, 1547, 1813
PspPPI RGGWCCY 2 cut(s) 487, 1813
PspXI VCTCGAGB 1 cut(s) 146
PsuI RGATCY 4 cut(s) 664, 727, 791, 919
RsaI GTAC 4 cut(s) 48, 949, 1446, 1635
RsaNI GTAC 4 cut(s) 47, 948, 1445, 1634
RseI CAYNNNNRTG 3 cut(s) 857, 1047, 1253
SaqAI TTAA 9 cut(s) 183, 213, 353, 708, 833, 1035, 1383, 1572, 1856
SatI GCNGC 4 cut(s) 414, 672, 675, 1161
Sau3AI GATC 7 cut(s) 141, 151, 307, 664, 727, 791, 919
Sau96I GGNCC 8 cut(s) 393, 394, 476, 487, 554, 1358, 1547, 1813
SchI GAGTC 3 cut(s) 161, 388, 1295
ScrFI CCNGG 7 cut(s) 843, 1094, 1223, 1416, 1605, 1696, 1809
SduI GDGCHC 3 cut(s) 397, 814, 1054
SfcI CTRYAG 3 cut(s) 511, 960, 1755
Sfr274I CTCGAG 1 cut(s) 146
SinI GGWCC 4 cut(s) 476, 487, 554, 1813
SlaI CTCGAG 1 cut(s) 146
SmiMI CAYNNNNRTG 3 cut(s) 857, 1047, 1253
SmlI CTYRAG 2 cut(s) 146, 866
SmoI CTYRAG 2 cut(s) 146, 866
SpeI ACTAGT 1 cut(s) 1778
SsiI CCGC 3 cut(s) 202, 674, 1299
SspMI CTAG 4 cut(s) 752, 890, 1290, 1779
StyD4I CCNGG 7 cut(s) 841, 1092, 1221, 1414, 1603, 1694, 1807
StyI CCWWGG 2 cut(s) 472, 805
TaaI ACNGT 7 cut(s) 288, 315, 1049, 1437, 1731, 1848, 1869
TaiI ACGT 3 cut(s) 192, 201, 434
TaqI TCGA 6 cut(s) 147, 249, 310, 382, 1321, 1510
TatI WGTACW 2 cut(s) 1444, 1633
TauI GCSGC 1 cut(s) 677
TfiI GAWTC 5 cut(s) 13, 965, 1244, 1279, 1679
Tru1I TTAA 9 cut(s) 183, 213, 353, 708, 833, 1035, 1383, 1572, 1856
Tru9I TTAA 9 cut(s) 183, 213, 353, 708, 833, 1035, 1383, 1572, 1856
TscAI CASTG 5 cut(s) 291, 1233, 1440, 1602, 1734
TseFI GTSAC 3 cut(s) 589, 880, 1731
TseI GCWGC 3 cut(s) 413, 671, 1160
Tsp45I GTSAC 3 cut(s) 589, 880, 1731
TspDTI ATGAA 7 cut(s) 17, 449, 453, 711, 1325, 1514, 1776
TspGWI ACGGA 1 cut(s) 1212
TspRI CASTG 5 cut(s) 291, 1233, 1440, 1602, 1734
VpaK11BI GGWCC 4 cut(s) 476, 487, 554, 1813
VspI ATTAAT 1 cut(s) 833
XagI CCTNNNNNAGG 1 cut(s) 626
XapI RAATTY 9 cut(s) 384, 506, 614, 698, 775, 1087, 1400, 1589, 1714
XbaI TCTAGA 1 cut(s) 889
XceI RCATGY 1 cut(s) 1009
XhoI CTCGAG 1 cut(s) 146
XmiI GTMKAC 1 cut(s) 1065
XmnI GAANNNNTTC 2 cut(s) 615, 1371
XspI CTAG 4 cut(s) 752, 890, 1290, 1779
ZraI GACGTC 1 cut(s) 190
Zsp2I ATGCAT 2 cut(s) 230, 1024
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.