Rorug01G0368300

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0001135
Physical Location & Seq
Forward (+)
48334645 .. 48335275
631 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0368300.1

Sequence Viewer

Length: 351 bp
ATGGCAGATGGCGGTTCAATATGTGGTTGTGCTTGGTCTTCTTGCCTTGTAATCTTGTGCTTTTCTCGATCTTCTGATGATGGAAGGTTAACTGATGGACAAGGTGTTGTCGTGGACTTCAGAAATACTTTGATTATTCTGACCTCTAACCTTGGAGCAAAGCATCTCCTTTCTGGTTTAATAGGAGAGTGTACAATGAAAGATGCTCGCGACCTAGTTATGCCAGAGGTGAGAAGTCACTTTAGTCCTGAATTACTAAATCGGCTTGATGAGATTGTCATATTTGATCCTCTTTCACACGAGCAATTAAAGAAGGTGGCCAGGTTGCAGATGAAGGATGTTGCTGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2 PAP_assoc

Protein Analysis

116

Amino Acids

12.69

Weight (kDa)

5.49

Isoelectric Point (pI)

49.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_2 PF07724 26 - 92 2.5e-15 AAA domain (Cdc48 subfamily)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 210
AciI CCGC 1 cut(s) 12
AclWI GGATC 1 cut(s) 281
AcoI YGGCCR 1 cut(s) 318
AcuI CTGAAG 1 cut(s) 103
AfaI GTAC 1 cut(s) 193
AgsI TTSAA 1 cut(s) 18
AjnI CCWGG 1 cut(s) 320
AlwI GGATC 1 cut(s) 281
AoxI GGCC 1 cut(s) 318
AsuHPI GGTGA 1 cut(s) 241
BalI TGGCCA 1 cut(s) 320
BauI CACGAG 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 30
BccI CCATC 3 cut(s) 2, 74, 89
BciT130I CCWGG 1 cut(s) 322
BfaI CTAG 1 cut(s) 215
Bme1390I CCNGG 1 cut(s) 322
BmrFI CCNGG 1 cut(s) 322
BmsI GCATC 2 cut(s) 172, 193
BpiI GAAGAC 1 cut(s) 30
BsaJI CCNNGG 1 cut(s) 151
BseBI CCWGG 1 cut(s) 322
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 1 cut(s) 343
Bsh1236I CGCG 1 cut(s) 210
BshFI GGCC 1 cut(s) 320
BsnI GGCC 1 cut(s) 320
Bsp1407I TGTACA 1 cut(s) 191
Bsp143I GATC 2 cut(s) 68, 286
Bsp68I TCGCGA 1 cut(s) 210
BspACI CCGC 1 cut(s) 12
BspANI GGCC 1 cut(s) 320
BspFNI CGCG 1 cut(s) 210
BspPI GGATC 1 cut(s) 281
BsrGI TGTACA 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 2 cut(s) 68, 286
BssSI CACGAG 1 cut(s) 299
BssT1I CCWWGG 1 cut(s) 151
Bst2BI CACGAG 1 cut(s) 299
Bst2UI CCWGG 1 cut(s) 322
BstAUI TGTACA 1 cut(s) 191
BstC8I GCNNGC 1 cut(s) 208
BstF5I GGATG 1 cut(s) 343
BstFNI CGCG 1 cut(s) 210
BstKTI GATC 2 cut(s) 71, 289
BstMBI GATC 2 cut(s) 68, 286
BstNI CCWGG 1 cut(s) 322
BstSCI CCNGG 1 cut(s) 320
BstUI CGCG 1 cut(s) 210
BstV2I GAAGAC 1 cut(s) 30
BsuRI GGCC 1 cut(s) 320
BtsCI GGATG 1 cut(s) 343
BtuMI TCGCGA 1 cut(s) 210
Cac8I GCNNGC 1 cut(s) 208
Csp6I GTAC 1 cut(s) 192
CviJI RGCY 2 cut(s) 265, 320
CviKI_1 RGCY 2 cut(s) 265, 320
CviQI GTAC 1 cut(s) 192
DpnI GATC 2 cut(s) 70, 288
DpnII GATC 2 cut(s) 68, 286
EaeI YGGCCR 1 cut(s) 318
Eco130I CCWWGG 1 cut(s) 151
Eco57I CTGAAG 1 cut(s) 103
EcoRII CCWGG 1 cut(s) 320
EcoT14I CCWWGG 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 151
FaiI YATR 3 cut(s) 22, 221, 281
FspBI CTAG 1 cut(s) 215
HaeIII GGCC 1 cut(s) 320
HincII GTYRAC 1 cut(s) 90
HindII GTYRAC 1 cut(s) 90
HpaI GTTAAC 1 cut(s) 90
HphI GGTGA 1 cut(s) 241
Hpy166II GTNNAC 3 cut(s) 90, 115, 192
Hpy188I TCNGA 4 cut(s) 76, 122, 141, 350
Hpy188III TCNNGA 3 cut(s) 66, 209, 248
Hpy8I GTNNAC 3 cut(s) 90, 115, 192
HpyAV CCTTC 3 cut(s) 78, 307, 328
HpyCH4V TGCA 1 cut(s) 328
KspAI GTTAAC 1 cut(s) 90
Kzo9I GATC 2 cut(s) 68, 286
LmnI GCTCC 1 cut(s) 155
LpnPI CCDG 5 cut(s) 159, 237, 261, 307, 334
LweI GCATC 2 cut(s) 172, 193
MaeI CTAG 1 cut(s) 215
MaeIII GTNAC 1 cut(s) 236
MalI GATC 2 cut(s) 70, 288
MboI GATC 2 cut(s) 68, 286
MboII GAAGA 2 cut(s) 30, 63
MlsI TGGCCA 1 cut(s) 320
MluCI AATT 2 cut(s) 251, 305
MluNI TGGCCA 1 cut(s) 320
MnlI CCTC 3 cut(s) 154, 220, 300
Mox20I TGGCCA 1 cut(s) 320
MscI TGGCCA 1 cut(s) 320
MseI TTAA 3 cut(s) 89, 179, 308
Msp20I TGGCCA 1 cut(s) 320
MspR9I CCNGG 1 cut(s) 322
MvaI CCWGG 1 cut(s) 322
MvnI CGCG 1 cut(s) 210
NdeII GATC 2 cut(s) 68, 286
NmuCI GTSAC 1 cut(s) 236
NruI TCGCGA 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 320
PspGI CCWGG 1 cut(s) 320
RruI TCGCGA 1 cut(s) 210
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
SaqAI TTAA 3 cut(s) 89, 179, 308
Sau3AI GATC 2 cut(s) 68, 286
ScrFI CCNGG 1 cut(s) 322
SetI ASST 8 cut(s) 89, 106, 146, 153, 216, 231, 318, 326
SfaNI GCATC 2 cut(s) 172, 193
Sse9I AATT 2 cut(s) 251, 305
SsiI CCGC 1 cut(s) 12
SspMI CTAG 1 cut(s) 215
StyD4I CCNGG 1 cut(s) 320
StyI CCWWGG 1 cut(s) 151
TaqI TCGA 1 cut(s) 67
TasI AATT 2 cut(s) 251, 305
TatI WGTACW 1 cut(s) 191
Tru1I TTAA 3 cut(s) 89, 179, 308
Tru9I TTAA 3 cut(s) 89, 179, 308
TseFI GTSAC 1 cut(s) 236
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 2 cut(s) 212, 347
XspI CTAG 1 cut(s) 215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.