isoform X1
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 321 |
| AccB1I | GGYRCC | 1 cut(s) | 611 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 824 |
| AccBSI | CCGCTC | 1 cut(s) | 127 |
| AccI | GTMKAC | 1 cut(s) | 873 |
| AccIII | TCCGGA | 1 cut(s) | 280 |
| AciI | CCGC | 4 cut(s) | 71, 127, 167, 356 |
| AclWI | GGATC | 3 cut(s) | 80, 944, 1758 |
| AcoI | YGGCCR | 2 cut(s) | 976, 1219 |
| AcsI | RAATTY | 3 cut(s) | 47, 737, 1859 |
| AcuI | CTGAAG | 4 cut(s) | 383, 715, 831, 1889 |
| AfaI | GTAC | 4 cut(s) | 502, 816, 1457, 1794 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 703, 824, 1517, 1870 |
| AjnI | CCWGG | 4 cut(s) | 6, 581, 1510, 1609 |
| AleI | CACNNNNGTG | 2 cut(s) | 898, 1227 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1531, 1563 |
| Alw21I | GWGCWC | 2 cut(s) | 1036, 1632 |
| Alw26I | GTCTC | 1 cut(s) | 1552 |
| AlwI | GGATC | 3 cut(s) | 80, 944, 1758 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1640 |
| Aor13HI | TCCGGA | 1 cut(s) | 280 |
| AoxI | GGCC | 5 cut(s) | 101, 114, 694, 976, 1219 |
| ApeKI | GCWGC | 6 cut(s) | 322, 386, 561, 841, 1532, 1586 |
| ApoI | RAATTY | 3 cut(s) | 47, 737, 1859 |
| AseI | ATTAAT | 1 cut(s) | 1335 |
| AspLEI | GCGC | 1 cut(s) | 322 |
| AspS9I | GGNCC | 4 cut(s) | 101, 114, 695, 706 |
| AsuC2I | CCSGG | 3 cut(s) | 62, 137, 1649 |
| AsuHPI | GGTGA | 6 cut(s) | 167, 1184, 1230, 1260, 1599, 1888 |
| AsuII | TTCGAA | 1 cut(s) | 862 |
| AvaII | GGWCC | 1 cut(s) | 706 |
| BaeGI | GKGCMC | 1 cut(s) | 616 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1364, 1397 |
| BalI | TGGCCA | 1 cut(s) | 1221 |
| BanI | GGYRCC | 1 cut(s) | 611 |
| BanII | GRGCYC | 2 cut(s) | 1036, 1632 |
| BauI | CACGAG | 1 cut(s) | 1440 |
| Bbv12I | GWGCWC | 2 cut(s) | 1036, 1632 |
| BbvCI | CCTCAGC | 3 cut(s) | 426, 1272, 1695 |
| BbvI | GCAGC | 6 cut(s) | 334, 373, 573, 828, 1544, 1598 |
| BccI | CCATC | 9 cut(s) | 278, 420, 624, 678, 686, 898, 1695, 1786, 1872 |
| BceAI | ACGGC | 3 cut(s) | 17, 180, 963 |
| BciT130I | CCWGG | 4 cut(s) | 8, 583, 1512, 1611 |
| BclI | TGATCA | 1 cut(s) | 526 |
| BcnI | CCSGG | 3 cut(s) | 62, 137, 1649 |
| BcoDI | GTCTC | 1 cut(s) | 1552 |
| BfaI | CTAG | 3 cut(s) | 689, 1043, 1761 |
| BfmI | CTRYAG | 3 cut(s) | 712, 1316, 1584 |
| BisI | GCNGC | 8 cut(s) | 71, 168, 323, 387, 562, 842, 1533, 1587 |
| BlsI | GCNGC | 8 cut(s) | 72, 169, 324, 388, 563, 843, 1534, 1588 |
| Bme1390I | CCNGG | 7 cut(s) | 8, 62, 137, 583, 1512, 1611, 1649 |
| Bme18I | GGWCC | 1 cut(s) | 706 |
| BmgT120I | GGNCC | 4 cut(s) | 101, 114, 695, 706 |
| BmiI | GGNNCC | 6 cut(s) | 24, 613, 697, 1396, 1540, 1713 |
| BmrFI | CCNGG | 7 cut(s) | 8, 62, 137, 583, 1512, 1611, 1649 |
| BmrI | ACTGGG | 1 cut(s) | 1248 |
| BmsI | GCATC | 3 cut(s) | 412, 573, 943 |
| BmuI | ACTGGG | 1 cut(s) | 1248 |
| BoxI | GACNNNNGTC | 1 cut(s) | 8 |
| BpmI | CTGGAG | 2 cut(s) | 1578, 1632 |
| Bpu10I | CCTNAGC | 3 cut(s) | 426, 1272, 1695 |
| Bpu14I | TTCGAA | 1 cut(s) | 862 |
| BpuEI | CTTGAG | 1 cut(s) | 347 |
| BpuMI | CCSGG | 3 cut(s) | 62, 137, 1649 |
| Bsa29I | ATCGAT | 2 cut(s) | 948, 1833 |
| BsaBI | GATNNNNATC | 2 cut(s) | 411, 1686 |
| BsaJI | CCNNGG | 4 cut(s) | 6, 162, 973, 1292 |
| BsaWI | WCCGGW | 2 cut(s) | 177, 280 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1225, 1255, 1531, 1561 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 703, 824, 1517, 1870 |
| Bse118I | RCCGGY | 1 cut(s) | 1200 |
| Bse1I | ACTGG | 3 cut(s) | 316, 1254, 1812 |
| Bse3DI | GCAATG | 2 cut(s) | 65, 1283 |
| Bse8I | GATNNNNATC | 2 cut(s) | 411, 1686 |
| BseAI | TCCGGA | 1 cut(s) | 280 |
| BseBI | CCWGG | 4 cut(s) | 8, 583, 1512, 1611 |
| BseCI | ATCGAT | 2 cut(s) | 948, 1833 |
| BseDI | CCNNGG | 4 cut(s) | 6, 162, 973, 1292 |
| BseGI | GGATG | 8 cut(s) | 196, 289, 337, 412, 838, 1303, 1458, 1879 |
| BseJI | GATNNNNATC | 2 cut(s) | 411, 1686 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 703, 824, 1517, 1870 |
| BseMI | GCAATG | 2 cut(s) | 65, 1283 |
| BseMII | CTCAG | 6 cut(s) | 404, 417, 1286, 1647, 1709, 1746 |
| BseNI | ACTGG | 3 cut(s) | 316, 1254, 1812 |
| BseRI | GAGGAG | 3 cut(s) | 386, 1759, 1764 |
| BseSI | GKGCMC | 1 cut(s) | 616 |
| BseXI | GCAGC | 6 cut(s) | 334, 373, 573, 828, 1544, 1598 |
| BseYI | CCCAGC | 1 cut(s) | 1323 |
| BsgI | GTGCAG | 2 cut(s) | 227, 1166 |
| BshFI | GGCC | 5 cut(s) | 103, 116, 696, 978, 1221 |
| BshNI | GGYRCC | 1 cut(s) | 611 |
| BshVI | ATCGAT | 2 cut(s) | 948, 1833 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1036, 1632 |
| BslFI | GGGAC | 4 cut(s) | 39, 1306, 1465, 1473 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 703, 824, 1517, 1870 |
| BsmAI | GTCTC | 1 cut(s) | 1552 |
| BsmFI | GGGAC | 4 cut(s) | 39, 1306, 1465, 1473 |
| BsmI | GAATGC | 2 cut(s) | 603, 1622 |
| BsnI | GGCC | 5 cut(s) | 103, 116, 696, 978, 1221 |
| Bsp119I | TTCGAA | 1 cut(s) | 862 |
| Bsp1286I | GDGCHC | 3 cut(s) | 616, 1036, 1632 |
| Bsp13I | TCCGGA | 1 cut(s) | 280 |
| Bsp1407I | TGTACA | 1 cut(s) | 500 |
| Bsp143I | GATC | 6 cut(s) | 85, 526, 544, 949, 1431, 1763 |
| Bsp19I | CCATGG | 1 cut(s) | 1292 |
| BspACI | CCGC | 4 cut(s) | 71, 127, 167, 356 |
| BspANI | GGCC | 5 cut(s) | 103, 116, 696, 978, 1221 |
| BspCNI | CTCAG | 6 cut(s) | 403, 418, 1285, 1646, 1708, 1747 |
| BspDI | ATCGAT | 2 cut(s) | 948, 1833 |
| BspEI | TCCGGA | 1 cut(s) | 280 |
| BspLI | GGNNCC | 6 cut(s) | 24, 613, 697, 1396, 1540, 1713 |
| BspMAI | CTGCAG | 1 cut(s) | 1588 |
| BspPI | GGATC | 3 cut(s) | 80, 944, 1758 |
| BspQI | GCTCTTC | 1 cut(s) | 1845 |
| BspT104I | TTCGAA | 1 cut(s) | 862 |
| BspT107I | GGYRCC | 1 cut(s) | 611 |
| BsrBI | CCGCTC | 1 cut(s) | 127 |
| BsrDI | GCAATG | 2 cut(s) | 65, 1283 |
| BsrFI | RCCGGY | 1 cut(s) | 1200 |
| BsrGI | TGTACA | 1 cut(s) | 500 |
| BsrI | ACTGG | 3 cut(s) | 316, 1254, 1812 |
| BssAI | RCCGGY | 1 cut(s) | 1200 |
| BssECI | CCNNGG | 4 cut(s) | 6, 162, 973, 1292 |
| BssMI | GATC | 6 cut(s) | 85, 526, 544, 949, 1431, 1763 |
| BssNAI | GTATAC | 1 cut(s) | 874 |
| BssSI | CACGAG | 1 cut(s) | 1440 |
| BssT1I | CCWWGG | 2 cut(s) | 973, 1292 |
| Bst1107I | GTATAC | 1 cut(s) | 874 |
| Bst2BI | CACGAG | 1 cut(s) | 1440 |
| Bst2UI | CCWGG | 4 cut(s) | 8, 583, 1512, 1611 |
| Bst4CI | ACNGT | 9 cut(s) | 304, 706, 869, 1144, 1187, 1320, 1351, 1814, 1821 |
| Bst6I | CTCTTC | 3 cut(s) | 846, 1419, 1845 |
| BstAUI | TGTACA | 1 cut(s) | 500 |
| BstBI | TTCGAA | 1 cut(s) | 862 |
| BstC8I | GCNNGC | 4 cut(s) | 125, 129, 1036, 1040 |
| BstDEI | CTNAG | 8 cut(s) | 390, 426, 506, 845, 1272, 1633, 1695, 1755 |
| BstDSI | CCRYGG | 2 cut(s) | 162, 1292 |
| BstF5I | GGATG | 8 cut(s) | 196, 289, 337, 412, 838, 1303, 1458, 1879 |
| BstHHI | GCGC | 1 cut(s) | 322 |
| BstKTI | GATC | 6 cut(s) | 88, 529, 547, 952, 1434, 1766 |
| BstMAI | GTCTC | 1 cut(s) | 1552 |
| BstMBI | GATC | 6 cut(s) | 85, 526, 544, 949, 1431, 1763 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1044 |
| BstNI | CCWGG | 4 cut(s) | 8, 583, 1512, 1611 |
| BstNSI | RCATGY | 1 cut(s) | 1379 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 8 |
| BstSCI | CCNGG | 7 cut(s) | 6, 60, 135, 581, 1510, 1609, 1647 |
| BstSFI | CTRYAG | 3 cut(s) | 712, 1316, 1584 |
| BstSLI | GKGCMC | 1 cut(s) | 616 |
| BstV1I | GCAGC | 6 cut(s) | 334, 373, 573, 828, 1544, 1598 |
| BstX2I | RGATCY | 1 cut(s) | 1763 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1727 |
| BstYI | RGATCY | 1 cut(s) | 1763 |
| BstZ17I | GTATAC | 1 cut(s) | 874 |
| Bsu15I | ATCGAT | 2 cut(s) | 948, 1833 |
| BsuRI | GGCC | 5 cut(s) | 103, 116, 696, 978, 1221 |
| BsuTUI | ATCGAT | 2 cut(s) | 948, 1833 |
| BtgI | CCRYGG | 2 cut(s) | 162, 1292 |
| BtgZI | GCGATG | 1 cut(s) | 398 |
| BtsCI | GGATG | 8 cut(s) | 196, 289, 337, 412, 838, 1303, 1458, 1879 |
| BtsI | GCAGTG | 1 cut(s) | 322 |
| BtsIMutI | CAGTG | 2 cut(s) | 309, 322 |
| Cac8I | GCNNGC | 4 cut(s) | 125, 129, 1036, 1040 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1640 |
| CfoI | GCGC | 1 cut(s) | 322 |
| Cfr10I | RCCGGY | 1 cut(s) | 1200 |
| Cfr13I | GGNCC | 4 cut(s) | 101, 114, 695, 706 |
| ClaI | ATCGAT | 2 cut(s) | 948, 1833 |
| Csp6I | GTAC | 4 cut(s) | 501, 815, 1456, 1793 |
| CviQI | GTAC | 4 cut(s) | 501, 815, 1456, 1793 |
| DdeI | CTNAG | 8 cut(s) | 390, 426, 506, 845, 1272, 1633, 1695, 1755 |
| DpnI | GATC | 6 cut(s) | 87, 528, 546, 951, 1433, 1765 |
| DpnII | GATC | 6 cut(s) | 85, 526, 544, 949, 1431, 1763 |
| DraI | TTTAAA | 2 cut(s) | 768, 1024 |
| EaeI | YGGCCR | 2 cut(s) | 976, 1219 |
| Eam1104I | CTCTTC | 3 cut(s) | 846, 1419, 1845 |
| EarI | CTCTTC | 3 cut(s) | 846, 1419, 1845 |
| Ecl136II | GAGCTC | 2 cut(s) | 1034, 1630 |
| Eco130I | CCWWGG | 2 cut(s) | 973, 1292 |
| Eco24I | GRGCYC | 2 cut(s) | 1036, 1632 |
| Eco47I | GGWCC | 1 cut(s) | 706 |
| Eco53kI | GAGCTC | 2 cut(s) | 1034, 1630 |
| Eco57I | CTGAAG | 4 cut(s) | 383, 715, 831, 1889 |
| EcoICRI | GAGCTC | 2 cut(s) | 1034, 1630 |
| EcoRI | GAATTC | 1 cut(s) | 1859 |
| EcoRII | CCWGG | 4 cut(s) | 6, 581, 1510, 1609 |
| EcoT14I | CCWWGG | 2 cut(s) | 973, 1292 |
| EcoT22I | ATGCAT | 2 cut(s) | 449, 1931 |
| EcoT38I | GRGCYC | 2 cut(s) | 1036, 1632 |
| ErhI | CCWWGG | 2 cut(s) | 973, 1292 |
| FaqI | GGGAC | 4 cut(s) | 39, 1306, 1465, 1473 |
| FauI | CCCGC | 1 cut(s) | 120 |
| FbaI | TGATCA | 1 cut(s) | 526 |
| FblI | GTMKAC | 1 cut(s) | 873 |
| Fnu4HI | GCNGC | 8 cut(s) | 71, 168, 323, 387, 562, 842, 1533, 1587 |
| FokI | GGATG | 8 cut(s) | 203, 296, 344, 419, 845, 1310, 1445, 1886 |
| FriOI | GRGCYC | 2 cut(s) | 1036, 1632 |
| Fsp4HI | GCNGC | 8 cut(s) | 71, 168, 323, 387, 562, 842, 1533, 1587 |
| FspBI | CTAG | 3 cut(s) | 689, 1043, 1761 |
| FspI | TGCGCA | 1 cut(s) | 321 |
| GlaI | GCGC | 1 cut(s) | 321 |
| GluI | GCNGC | 8 cut(s) | 71, 168, 323, 387, 562, 842, 1533, 1587 |
| GsaI | CCCAGC | 1 cut(s) | 1327 |
| GsuI | CTGGAG | 2 cut(s) | 1578, 1632 |
| HaeIII | GGCC | 5 cut(s) | 103, 116, 696, 978, 1221 |
| HhaI | GCGC | 1 cut(s) | 322 |
| Hin6I | GCGC | 1 cut(s) | 320 |
| HinP1I | GCGC | 1 cut(s) | 320 |
| HindIII | AAGCTT | 2 cut(s) | 434, 508 |
| HinfI | GANTC | 8 cut(s) | 107, 154, 172, 598, 1051, 1288, 1757, 1830 |
| HphI | GGTGA | 6 cut(s) | 167, 1184, 1230, 1260, 1599, 1888 |
| Hpy166II | GTNNAC | 3 cut(s) | 503, 874, 1817 |
| Hpy188III | TCNNGA | 6 cut(s) | 281, 515, 689, 1595, 1761, 1827 |
| Hpy8I | GTNNAC | 3 cut(s) | 503, 874, 1817 |
| Hpy99I | CGWCG | 2 cut(s) | 38, 94 |
| HpyCH4III | ACNGT | 9 cut(s) | 304, 706, 869, 1144, 1187, 1320, 1351, 1814, 1821 |
| HpyCH4IV | ACGT | 3 cut(s) | 228, 635, 1454 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1044 |
| HpyF3I | CTNAG | 8 cut(s) | 390, 426, 506, 845, 1272, 1633, 1695, 1755 |
| HpySE526I | ACGT | 3 cut(s) | 228, 635, 1454 |
| HspAI | GCGC | 1 cut(s) | 320 |
| Kpn2I | TCCGGA | 1 cut(s) | 280 |
| Ksp22I | TGATCA | 1 cut(s) | 526 |
| Kzo9I | GATC | 6 cut(s) | 85, 526, 544, 949, 1431, 1763 |
| LguI | GCTCTTC | 1 cut(s) | 1845 |
| LmnI | GCTCC | 5 cut(s) | 22, 1031, 1165, 1552, 1711 |
| Lsp1109I | GCAGC | 6 cut(s) | 334, 373, 573, 828, 1544, 1598 |
| LweI | GCATC | 3 cut(s) | 412, 573, 943 |
| MaeI | CTAG | 3 cut(s) | 689, 1043, 1761 |
| MaeII | ACGT | 3 cut(s) | 228, 635, 1454 |
| MaeIII | GTNAC | 5 cut(s) | 224, 1059, 1172, 1266, 1300 |
| MalI | GATC | 6 cut(s) | 87, 528, 546, 951, 1433, 1765 |
| MbiI | CCGCTC | 1 cut(s) | 127 |
| MboI | GATC | 6 cut(s) | 85, 526, 544, 949, 1431, 1763 |
| MboII | GAAGA | 9 cut(s) | 269, 658, 675, 863, 1436, 1441, 1799, 1862, 1889 |
| MflI | RGATCY | 1 cut(s) | 1763 |
| MhlI | GDGCHC | 3 cut(s) | 616, 1036, 1632 |
| MlsI | TGGCCA | 1 cut(s) | 1221 |
| MluNI | TGGCCA | 1 cut(s) | 1221 |
| MlyI | GAGTC | 4 cut(s) | 116, 166, 607, 1766 |
| MmeI | TCCRAC | 3 cut(s) | 72, 1143, 1761 |
| Mox20I | TGGCCA | 1 cut(s) | 1221 |
| Mph1103I | ATGCAT | 2 cut(s) | 449, 1931 |
| MroI | TCCGGA | 1 cut(s) | 280 |
| MscI | TGGCCA | 1 cut(s) | 1221 |
| MslI | CAYNNNNRTG | 5 cut(s) | 351, 898, 1227, 1389, 1725 |
| Msp20I | TGGCCA | 1 cut(s) | 1221 |
| MspA1I | CMGCKG | 1 cut(s) | 1535 |
| MspR9I | CCNGG | 7 cut(s) | 8, 62, 137, 583, 1512, 1611, 1649 |
| Mva1269I | GAATGC | 2 cut(s) | 603, 1622 |
| MvaI | CCWGG | 4 cut(s) | 8, 583, 1512, 1611 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1044 |
| NciI | CCSGG | 3 cut(s) | 62, 137, 1649 |
| NcoI | CCATGG | 1 cut(s) | 1292 |
| NdeII | GATC | 6 cut(s) | 85, 526, 544, 949, 1431, 1763 |
| NlaIV | GGNNCC | 6 cut(s) | 24, 613, 697, 1396, 1540, 1713 |
| NmuCI | GTSAC | 2 cut(s) | 1172, 1266 |
| NsbI | TGCGCA | 1 cut(s) | 321 |
| NsiI | ATGCAT | 2 cut(s) | 449, 1931 |
| NspI | RCATGY | 1 cut(s) | 1379 |
| NspV | TTCGAA | 1 cut(s) | 862 |
| OliI | CACNNNNGTG | 2 cut(s) | 898, 1227 |
| PciSI | GCTCTTC | 1 cut(s) | 1845 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 23 |
| PctI | GAATGC | 2 cut(s) | 603, 1622 |
| PfeI | GAWTC | 4 cut(s) | 154, 1051, 1288, 1830 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 824 |
| PfoI | TCCNGGA | 1 cut(s) | 135 |
| PkrI | GCNGC | 8 cut(s) | 72, 169, 324, 388, 563, 843, 1534, 1588 |
| PleI | GAGTC | 4 cut(s) | 115, 166, 606, 1765 |
| PpsI | GAGTC | 4 cut(s) | 115, 166, 606, 1765 |
| PshAI | GACNNNNGTC | 1 cut(s) | 8 |
| PshBI | ATTAAT | 1 cut(s) | 1335 |
| Psp124BI | GAGCTC | 2 cut(s) | 1036, 1632 |
| Psp6I | CCWGG | 4 cut(s) | 6, 581, 1510, 1609 |
| PspFI | CCCAGC | 1 cut(s) | 1323 |
| PspGI | CCWGG | 4 cut(s) | 6, 581, 1510, 1609 |
| PspN4I | GGNNCC | 6 cut(s) | 24, 613, 697, 1396, 1540, 1713 |
| PspPI | GGNCC | 4 cut(s) | 101, 114, 695, 706 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 333, 365 |
| PstI | CTGCAG | 1 cut(s) | 1588 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1640 |
| PsuI | RGATCY | 1 cut(s) | 1763 |
| PvuII | CAGCTG | 1 cut(s) | 1535 |
| RsaI | GTAC | 4 cut(s) | 502, 816, 1457, 1794 |
| RsaNI | GTAC | 4 cut(s) | 501, 815, 1456, 1793 |
| RseI | CAYNNNNRTG | 5 cut(s) | 351, 898, 1227, 1389, 1725 |
| SacI | GAGCTC | 2 cut(s) | 1036, 1632 |
| SapI | GCTCTTC | 1 cut(s) | 1845 |
| SatI | GCNGC | 8 cut(s) | 71, 168, 323, 387, 562, 842, 1533, 1587 |
| Sau3AI | GATC | 6 cut(s) | 85, 526, 544, 949, 1431, 1763 |
| Sau96I | GGNCC | 4 cut(s) | 101, 114, 695, 706 |
| SchI | GAGTC | 4 cut(s) | 116, 166, 607, 1766 |
| ScrFI | CCNGG | 7 cut(s) | 8, 62, 137, 583, 1512, 1611, 1649 |
| SduI | GDGCHC | 3 cut(s) | 616, 1036, 1632 |
| SfaNI | GCATC | 3 cut(s) | 412, 573, 943 |
| SfcI | CTRYAG | 3 cut(s) | 712, 1316, 1584 |
| SfuI | TTCGAA | 1 cut(s) | 862 |
| SinI | GGWCC | 1 cut(s) | 706 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 351, 898, 1227, 1389, 1725 |
| SmlI | CTYRAG | 1 cut(s) | 326 |
| SmoI | CTYRAG | 1 cut(s) | 326 |
| SsiI | CCGC | 4 cut(s) | 71, 127, 167, 356 |
| SspI | AATATT | 2 cut(s) | 802, 1333 |
| SspMI | CTAG | 3 cut(s) | 689, 1043, 1761 |
| SstI | GAGCTC | 2 cut(s) | 1036, 1632 |
| StyD4I | CCNGG | 7 cut(s) | 6, 60, 135, 581, 1510, 1609, 1647 |
| StyI | CCWWGG | 2 cut(s) | 973, 1292 |
| TaaI | ACNGT | 9 cut(s) | 304, 706, 869, 1144, 1187, 1320, 1351, 1814, 1821 |
| TaiI | ACGT | 3 cut(s) | 231, 638, 1457 |
| TaqI | TCGA | 4 cut(s) | 862, 948, 1006, 1833 |
| TatI | WGTACW | 1 cut(s) | 500 |
| TauI | GCSGC | 2 cut(s) | 73, 170 |
| TfiI | GAWTC | 4 cut(s) | 154, 1051, 1288, 1830 |
| TscAI | CASTG | 2 cut(s) | 316, 322 |
| TseFI | GTSAC | 2 cut(s) | 1172, 1266 |
| TseI | GCWGC | 6 cut(s) | 322, 386, 561, 841, 1532, 1586 |
| Tsp45I | GTSAC | 2 cut(s) | 1172, 1266 |
| TspDTI | ATGAA | 7 cut(s) | 335, 341, 1098, 1137, 1737, 1851, 1890 |
| TspGWI | ACGGA | 1 cut(s) | 78 |
| TspRI | CASTG | 2 cut(s) | 316, 322 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 824 |
| VpaK11BI | GGWCC | 1 cut(s) | 706 |
| VspI | ATTAAT | 1 cut(s) | 1335 |
| XapI | RAATTY | 3 cut(s) | 47, 737, 1859 |
| XbaI | TCTAGA | 2 cut(s) | 688, 1760 |
| XceI | RCATGY | 1 cut(s) | 1379 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1804 |
| XmiI | GTMKAC | 1 cut(s) | 873 |
| XspI | CTAG | 3 cut(s) | 689, 1043, 1761 |
| Zsp2I | ATGCAT | 2 cut(s) | 449, 1931 |