Rw5G044220

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001135
Physical Location & Seq
Reverse (-)
77833508 .. 77837508
4001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044220.1

Sequence Viewer

Length: 1899 bp
ATGACCCAGGTCTGTTCGCCGGAGCCGAACCGCGCCGTGTTGCCGGAATTTCGTTCCTACCCGTCCTCGCCGCCATTGCCGGCGACCAGTCCCGATCCGTCGGCAATTGGGCCGGAGTCTTGGGCCCGAGCCGAGCGGGCCACCCGGGATATACTGTCCAAGATTCAGCCCACGGCGGCGACTCACCGGAGAAGGAAGGATATCATTGTATATGTGCAGAGCCTCATCACTTGTAACGTGGGCTGTCAGGTTTTTCCTTATGGGTCAGTTCCATTAAAGACATATCTTCCCGATGGAGATATAGACTTGACGGTTTTCACTGGCAGTGCGCACCTTGAGGATGTCCTTGTAACTCATGTTCATGCTGTTCTGAAGGGGGAAGAGAATAATGAAGCTGCTCAGTATCAAGTGAAGGATGTCCATCGCATTGATGCTGAGGTTAAACTTGTAAAATGCATTGTTCAAAATATGATTGTAGATATATCCTTCAATCAGTTAGGAGGACTTTCTACACTACGCTTTCTTGAACAGGTTGATCACTATATTGGCAAAGATCATATTTTCAAACGCAGCATTATTCTGATAAAAGCCTGGTGCTACTATGAGAGTCGTATTCTTGGTGCTCATCATGGTTTAATTTCAACATATGCTTTGGAAACATTAGTCCTATATATATTCCATCTTTTTCATTCTTCTCTAGATGGCCCTTTAACGGTCCTCTATAGATTTTTGGATTACTTCAGCAAATTTGATTGGGAGAAATATTGTGTTAGTTTAAATGGACCAGTTTGCAAATCCTCCCTACCTGATATTGTGGCTGAAGTACCAGATAATGGAGGGGATGATGTACTGCTAAGTGAAGAGTTTATTAGAAACTGTGTAGACATGTTCTCTGTTCCATCACAGGGATGTGAGACAAACTTAAGAGTGTTTTCCTTGAAGCATCTTAATATAATCGATCCACTAAAAGAGAACAACAACCTTGGCCGTAGTGTCAATAGAGGTAGCTTCTATCGAATATGTAGTGCTTTTAAATATGGAGCTCGCAAGCTAGGCTGGATTCTCTTGTTACCAGCAGAGAGAATATCAGATGAACTTAACAAGTTTTTCGCCAACACTTTGGATAGGCATGAAAGTAAATGCCAGACTACTGTGCAAAATAATGCCTTGTTGGAGCTTGGTGAAGATTTGGACAGTCATTGTTTGAACCTGCAATATAGTCTGATTGGCCAGAGGTGTGCTACATCACCTCCTTTATTGCCCAGTCCTCCCTTGTCACCTCAACCATGGAAAGAGAATTCATGGGATACTACCTGCGAAGCCCTACAGTCCCAGCACAATATTAATTCTCAAACTAATGCAAATGGTATTCCTTGGGGAACACATGTGTATCACACAAATGGTGCCAGTTTTTTTGTTCCTCCATTCAGAGAAGAGAAGAACAAACCACGAGGAACAGGGACGTACATCCCCAATGCGTCTTTTCAGAATTATCGTCCCTACAAGGATAGACACTTGCCTGGAAGGGGAAGGAAAGAGGCAGCTGGAACTTGTGCTCCATTTCAGAGACACACTTTCAATTATGGCTTTCCTGCAGCCTCTCAAGAGTCAATCGCACCTGGAGAATGCAACCATGAGCTCTCAGAAGCTGAATACCCACTTTCAATTTGGGAGCCTTTCCATGAAAATGGTTTCTCCAAATCTCCCGACAAACCTGAGTCTAGATCCTCCTCCCCTCAATCATGGGAAGAAGTACCCATGCCACAACTGGACAGTTTACGGTTATCAGGAGTCGATGAAGAGAGGATTGACGAGCAGGCATTCCATCTGAAGGATGAAGCTGATTTCCCCCCGCTCACCCCTCGACTGCCTTTGAAGGATGGAATATGCATTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAP_assoc

Protein Analysis

632

Amino Acids

71.17

Weight (kDa)

5.83

Isoelectric Point (pI)

44.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 48 - 175 6.8e-08 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
PAP-OAS1 PF26180 182 - 374 6.8e-100 PAP/OAS1 substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 330
Acc36I ACCTGC 2 cut(s) 1218, 1322
AccB1I GGYRCC 1 cut(s) 1403
AccB7I CCANNNNNTGG 1 cut(s) 833
AccBSI CCGCTC 2 cut(s) 136, 1855
AccI GTMKAC 1 cut(s) 882
AccII CGCG 1 cut(s) 33
AciI CCGC 5 cut(s) 31, 71, 136, 176, 1853
AclWI GGATC 3 cut(s) 89, 953, 1719
AcoI YGGCCR 2 cut(s) 985, 1228
AcsI RAATTY 3 cut(s) 47, 746, 1297
AcuI CTGAAG 4 cut(s) 392, 724, 840, 1850
AfaI GTAC 4 cut(s) 825, 849, 1466, 1755
AfiI CCNNNNNNNGG 5 cut(s) 712, 833, 905, 1526, 1831
AflII CTTAAG 1 cut(s) 922
AflIII ACRYGT 2 cut(s) 885, 1384
AjnI CCWGG 4 cut(s) 6, 590, 1519, 1618
AloI GAACNNNNNNTCC 2 cut(s) 1540, 1572
AluBI AGCT 9 cut(s) 395, 1008, 1043, 1051, 1177, 1544, 1639, 1649, 1841
AluI AGCT 9 cut(s) 395, 1008, 1043, 1051, 1177, 1544, 1639, 1649, 1841
Alw21I GWGCWC 4 cut(s) 625, 1045, 1558, 1641
Alw26I GTCTC 2 cut(s) 908, 1561
AlwI GGATC 3 cut(s) 89, 953, 1719
AlwNI CAGNNNCTG 1 cut(s) 1649
Ama87I CYCGRG 2 cut(s) 126, 144
AoxI GGCC 6 cut(s) 110, 123, 138, 703, 985, 1228
ApaI GGGCCC 1 cut(s) 127
ApeKI GCWGC 4 cut(s) 395, 570, 1541, 1595
ApoI RAATTY 3 cut(s) 47, 746, 1297
AseI ATTAAT 1 cut(s) 1344
AspLEI GCGC 2 cut(s) 35, 331
AspS9I GGNCC 7 cut(s) 110, 123, 124, 138, 704, 715, 782
AsuC2I CCSGG 2 cut(s) 145, 146
AsuHPI GGTGA 5 cut(s) 176, 1193, 1239, 1269, 1849
AvaI CYCGRG 2 cut(s) 126, 144
AvaII GGWCC 2 cut(s) 715, 782
BaeGI GKGCMC 1 cut(s) 127
BaeI ACNNNNGTAYC 2 cut(s) 1373, 1406
BalI TGGCCA 1 cut(s) 1230
BanI GGYRCC 1 cut(s) 1403
BanII GRGCYC 3 cut(s) 127, 1045, 1641
BauI CACGAG 1 cut(s) 1449
Bbv12I GWGCWC 4 cut(s) 625, 1045, 1558, 1641
BbvCI CCTCAGC 1 cut(s) 435
BbvI GCAGC 4 cut(s) 382, 582, 1553, 1607
BccI CCATC 7 cut(s) 287, 429, 687, 695, 907, 1833, 1874
BceAI ACGGC 3 cut(s) 20, 189, 972
BciT130I CCWGG 4 cut(s) 8, 592, 1521, 1620
BciVI GTATCC 1 cut(s) 1300
BclI TGATCA 1 cut(s) 535
BcnI CCSGG 2 cut(s) 145, 146
BcoDI GTCTC 2 cut(s) 908, 1561
BfaI CTAG 3 cut(s) 698, 1052, 1722
BfmI CTRYAG 3 cut(s) 721, 1325, 1593
BfrI CTTAAG 1 cut(s) 922
BfuAI ACCTGC 2 cut(s) 1218, 1322
BfuI GTATCC 1 cut(s) 1300
BglI GCCNNNNNGGC 1 cut(s) 137
BisI GCNGC 6 cut(s) 71, 177, 396, 571, 1542, 1596
BlsI GCNGC 6 cut(s) 72, 178, 397, 572, 1543, 1597
Bme1390I CCNGG 6 cut(s) 8, 145, 146, 592, 1521, 1620
Bme18I GGWCC 2 cut(s) 715, 782
BmeT110I CYCGRG 2 cut(s) 126, 144
BmgT120I GGNCC 7 cut(s) 110, 123, 124, 138, 704, 715, 782
BmiI GGNNCC 4 cut(s) 24, 125, 1405, 1674
BmrFI CCNGG 6 cut(s) 8, 145, 146, 592, 1521, 1620
BmrI ACTGGG 1 cut(s) 1257
BmsI GCATC 2 cut(s) 421, 952
BmuI ACTGGG 1 cut(s) 1257
BoxI GACNNNNGTC 1 cut(s) 8
BpmI CTGGAG 1 cut(s) 1641
Bpu10I CCTNAGC 1 cut(s) 435
BpuEI CTTGAG 2 cut(s) 356, 1587
BpuMI CCSGG 2 cut(s) 145, 146
Bsa29I ATCGAT 1 cut(s) 957
BsaBI GATNNNNATC 1 cut(s) 420
BsaJI CCNNGG 6 cut(s) 6, 144, 171, 982, 1286, 1373
BsaWI WCCGGW 1 cut(s) 186
BsaXI ACNNNNNCTCC 4 cut(s) 1234, 1264, 1540, 1570
Bsc4I CCNNNNNNNGG 5 cut(s) 712, 833, 905, 1526, 1831
Bse118I RCCGGY 1 cut(s) 79
Bse1I ACTGG 6 cut(s) 87, 325, 785, 1263, 1407, 1773
Bse3DI GCAATG 1 cut(s) 74
Bse8I GATNNNNATC 1 cut(s) 420
BseBI CCWGG 4 cut(s) 8, 592, 1521, 1620
BseCI ATCGAT 1 cut(s) 957
BseDI CCNNGG 6 cut(s) 6, 144, 171, 982, 1286, 1373
BseGI GGATG 7 cut(s) 346, 421, 847, 914, 1467, 1840, 1885
BseJI GATNNNNATC 1 cut(s) 420
BseLI CCNNNNNNNGG 5 cut(s) 712, 833, 905, 1526, 1831
BseMI GCAATG 1 cut(s) 74
BseMII CTCAG 4 cut(s) 413, 426, 1656, 1707
BseNI ACTGG 6 cut(s) 87, 325, 785, 1263, 1407, 1773
BseRI GAGGAG 1 cut(s) 1720
BseSI GKGCMC 1 cut(s) 127
BseXI GCAGC 4 cut(s) 382, 582, 1553, 1607
BseYI CCCAGC 1 cut(s) 1332
BsgI GTGCAG 1 cut(s) 236
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 6 cut(s) 112, 125, 140, 705, 987, 1230
BshNI GGYRCC 1 cut(s) 1403
BshVI ATCGAT 1 cut(s) 957
BsiHKAI GWGCWC 4 cut(s) 625, 1045, 1558, 1641
BsiHKCI CYCGRG 2 cut(s) 126, 144
BsiSI CCGG 6 cut(s) 20, 44, 80, 113, 145, 187
BslFI GGGAC 4 cut(s) 75, 1315, 1474, 1482
BslI CCNNNNNNNGG 5 cut(s) 712, 833, 905, 1526, 1831
BsmAI GTCTC 2 cut(s) 908, 1561
BsmFI GGGAC 4 cut(s) 75, 1315, 1474, 1482
BsmI GAATGC 3 cut(s) 1631, 1820, 1890
BsnI GGCC 6 cut(s) 112, 125, 140, 705, 987, 1230
BsoBI CYCGRG 2 cut(s) 126, 144
Bsp120I GGGCCC 1 cut(s) 123
Bsp1286I GDGCHC 5 cut(s) 127, 625, 1045, 1558, 1641
Bsp143I GATC 5 cut(s) 94, 535, 553, 958, 1724
Bsp19I CCATGG 1 cut(s) 1286
BspACI CCGC 5 cut(s) 31, 71, 136, 176, 1853
BspANI GGCC 6 cut(s) 112, 125, 140, 705, 987, 1230
BspCNI CTCAG 4 cut(s) 412, 427, 1655, 1708
BspDI ATCGAT 1 cut(s) 957
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 4 cut(s) 24, 125, 1405, 1674
BspMAI CTGCAG 1 cut(s) 1597
BspMI ACCTGC 2 cut(s) 1218, 1322
BspPI GGATC 3 cut(s) 89, 953, 1719
BspT107I GGYRCC 1 cut(s) 1403
BspTI CTTAAG 1 cut(s) 922
BsrBI CCGCTC 2 cut(s) 136, 1855
BsrDI GCAATG 1 cut(s) 74
BsrFI RCCGGY 1 cut(s) 79
BsrI ACTGG 6 cut(s) 87, 325, 785, 1263, 1407, 1773
BssAI RCCGGY 1 cut(s) 79
BssECI CCNNGG 6 cut(s) 6, 144, 171, 982, 1286, 1373
BssMI GATC 5 cut(s) 94, 535, 553, 958, 1724
BssSI CACGAG 1 cut(s) 1449
BssT1I CCWWGG 3 cut(s) 982, 1286, 1373
Bst2BI CACGAG 1 cut(s) 1449
Bst2UI CCWGG 4 cut(s) 8, 592, 1521, 1620
Bst4CI ACNGT 9 cut(s) 156, 313, 715, 878, 1153, 1196, 1329, 1775, 1782
Bst6I CTCTTC 4 cut(s) 375, 855, 1428, 1794
BstAFI CTTAAG 1 cut(s) 922
BstC8I GCNNGC 5 cut(s) 81, 138, 1045, 1049, 1818
BstDEI CTNAG 5 cut(s) 399, 435, 854, 1642, 1716
BstDSI CCRYGG 2 cut(s) 171, 1286
BstF5I GGATG 7 cut(s) 346, 421, 847, 914, 1467, 1840, 1885
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 2 cut(s) 35, 331
BstKTI GATC 5 cut(s) 97, 538, 556, 961, 1727
BstMAI GTCTC 2 cut(s) 908, 1561
BstMBI GATC 5 cut(s) 94, 535, 553, 958, 1724
BstMWI GCNNNNNNNGC 3 cut(s) 76, 137, 1053
BstNI CCWGG 4 cut(s) 8, 592, 1521, 1620
BstNSI RCATGY 2 cut(s) 889, 1388
BstPAI GACNNNNGTC 1 cut(s) 8
BstSCI CCNGG 6 cut(s) 6, 143, 144, 590, 1519, 1618
BstSFI CTRYAG 3 cut(s) 721, 1325, 1593
BstSLI GKGCMC 1 cut(s) 127
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 4 cut(s) 382, 582, 1553, 1607
BstX2I RGATCY 1 cut(s) 1724
BstXI CCANNNNNNTGG 2 cut(s) 1120, 1688
BstYI RGATCY 1 cut(s) 1724
Bsu15I ATCGAT 1 cut(s) 957
BsuI GTATCC 1 cut(s) 1300
BsuRI GGCC 6 cut(s) 112, 125, 140, 705, 987, 1230
BsuTUI ATCGAT 1 cut(s) 957
BtgI CCRYGG 2 cut(s) 171, 1286
BtgZI GCGATG 1 cut(s) 407
BtsCI GGATG 7 cut(s) 346, 421, 847, 914, 1467, 1840, 1885
BtsI GCAGTG 1 cut(s) 331
BtsIMutI CAGTG 2 cut(s) 318, 331
BveI ACCTGC 2 cut(s) 1218, 1322
Cac8I GCNNGC 5 cut(s) 81, 138, 1045, 1049, 1818
CaiI CAGNNNCTG 1 cut(s) 1649
CfoI GCGC 2 cut(s) 35, 331
Cfr10I RCCGGY 1 cut(s) 79
Cfr13I GGNCC 7 cut(s) 110, 123, 124, 138, 704, 715, 782
Cfr9I CCCGGG 1 cut(s) 144
ClaI ATCGAT 1 cut(s) 957
CseI GACGC 1 cut(s) 1467
Csp6I GTAC 4 cut(s) 824, 848, 1465, 1754
CviQI GTAC 4 cut(s) 824, 848, 1465, 1754
DdeI CTNAG 5 cut(s) 399, 435, 854, 1642, 1716
DpnI GATC 5 cut(s) 96, 537, 555, 960, 1726
DpnII GATC 5 cut(s) 94, 535, 553, 958, 1724
DraI TTTAAA 2 cut(s) 777, 1033
EaeI YGGCCR 2 cut(s) 985, 1228
Eam1104I CTCTTC 4 cut(s) 375, 855, 1428, 1794
EarI CTCTTC 4 cut(s) 375, 855, 1428, 1794
Ecl136II GAGCTC 2 cut(s) 1043, 1639
Eco130I CCWWGG 3 cut(s) 982, 1286, 1373
Eco24I GRGCYC 3 cut(s) 127, 1045, 1641
Eco32I GATATC 1 cut(s) 202
Eco47I GGWCC 2 cut(s) 715, 782
Eco53kI GAGCTC 2 cut(s) 1043, 1639
Eco57I CTGAAG 4 cut(s) 392, 724, 840, 1850
Eco88I CYCGRG 2 cut(s) 126, 144
EcoICRI GAGCTC 2 cut(s) 1043, 1639
EcoRI GAATTC 1 cut(s) 1297
EcoRII CCWGG 4 cut(s) 6, 590, 1519, 1618
EcoRV GATATC 1 cut(s) 202
EcoT14I CCWWGG 3 cut(s) 982, 1286, 1373
EcoT22I ATGCAT 2 cut(s) 458, 1892
EcoT38I GRGCYC 3 cut(s) 127, 1045, 1641
ErhI CCWWGG 3 cut(s) 982, 1286, 1373
FaqI GGGAC 4 cut(s) 75, 1315, 1474, 1482
FauI CCCGC 2 cut(s) 129, 1860
FauNDI CATATG 1 cut(s) 646
FbaI TGATCA 1 cut(s) 535
FblI GTMKAC 1 cut(s) 882
Fnu4HI GCNGC 6 cut(s) 71, 177, 396, 571, 1542, 1596
FokI GGATG 7 cut(s) 353, 428, 854, 921, 1454, 1847, 1892
FriOI GRGCYC 3 cut(s) 127, 1045, 1641
Fsp4HI GCNGC 6 cut(s) 71, 177, 396, 571, 1542, 1596
FspAI RTGCGCAY 1 cut(s) 330
FspBI CTAG 3 cut(s) 698, 1052, 1722
FspI TGCGCA 1 cut(s) 330
GlaI GCGC 2 cut(s) 34, 330
GluI GCNGC 6 cut(s) 71, 177, 396, 571, 1542, 1596
GsaI CCCAGC 1 cut(s) 1336
GsuI CTGGAG 1 cut(s) 1641
HaeIII GGCC 6 cut(s) 112, 125, 140, 705, 987, 1230
HapII CCGG 6 cut(s) 20, 44, 80, 113, 145, 187
HgaI GACGC 1 cut(s) 1467
HhaI GCGC 2 cut(s) 35, 331
Hin6I GCGC 2 cut(s) 33, 329
HinP1I GCGC 2 cut(s) 33, 329
HinfI GANTC 8 cut(s) 116, 163, 181, 607, 1060, 1607, 1718, 1791
HpaII CCGG 6 cut(s) 20, 44, 80, 113, 145, 187
HphI GGTGA 5 cut(s) 176, 1193, 1239, 1269, 1849
Hpy166II GTNNAC 2 cut(s) 883, 1778
Hpy188I TCNGA 9 cut(s) 372, 582, 1090, 1224, 1430, 1488, 1566, 1645, 1830
Hpy188III TCNNGA 8 cut(s) 92, 290, 524, 698, 1604, 1706, 1722, 1788
Hpy8I GTNNAC 2 cut(s) 883, 1778
Hpy99I CGWCG 1 cut(s) 103
HpyAV CCTTC 9 cut(s) 186, 190, 367, 406, 496, 1518, 1524, 1825, 1870
HpyCH4III ACNGT 9 cut(s) 156, 313, 715, 878, 1153, 1196, 1329, 1775, 1782
HpyCH4IV ACGT 2 cut(s) 237, 1463
HpyCH4V TGCA 9 cut(s) 217, 456, 792, 1156, 1213, 1361, 1595, 1629, 1890
HpyF10VI GCNNNNNNNGC 3 cut(s) 76, 137, 1053
HpyF3I CTNAG 5 cut(s) 399, 435, 854, 1642, 1716
HpySE526I ACGT 2 cut(s) 237, 1463
HspAI GCGC 2 cut(s) 33, 329
KroI GCCGGC 1 cut(s) 79
KroNI GCCGGC 1 cut(s) 81
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 5 cut(s) 94, 535, 553, 958, 1724
LmnI GCTCC 5 cut(s) 22, 1040, 1174, 1561, 1672
Lsp1109I GCAGC 4 cut(s) 382, 582, 1553, 1607
LweI GCATC 2 cut(s) 421, 952
MaeI CTAG 3 cut(s) 698, 1052, 1722
MaeII ACGT 2 cut(s) 237, 1463
MaeIII GTNAC 4 cut(s) 233, 349, 1068, 1275
MalI GATC 5 cut(s) 96, 537, 555, 960, 1726
MbiI CCGCTC 2 cut(s) 136, 1855
MboI GATC 5 cut(s) 94, 535, 553, 958, 1724
MboII GAAGA 9 cut(s) 278, 392, 684, 872, 1196, 1445, 1450, 1760, 1811
MfeI CAATTG 1 cut(s) 105
MflI RGATCY 1 cut(s) 1724
MhlI GDGCHC 5 cut(s) 127, 625, 1045, 1558, 1641
MlsI TGGCCA 1 cut(s) 1230
MluCI AATT 9 cut(s) 47, 105, 636, 746, 1297, 1345, 1489, 1579, 1665
MluNI TGGCCA 1 cut(s) 1230
MlyI GAGTC 6 cut(s) 125, 175, 616, 1616, 1727, 1800
MmeI TCCRAC 1 cut(s) 1152
Mox20I TGGCCA 1 cut(s) 1230
Mph1103I ATGCAT 2 cut(s) 458, 1892
MroNI GCCGGC 1 cut(s) 79
MscI TGGCCA 1 cut(s) 1230
MslI CAYNNNNRTG 4 cut(s) 360, 907, 1398, 1686
Msp20I TGGCCA 1 cut(s) 1230
MspA1I CMGCKG 1 cut(s) 1544
MspCI CTTAAG 1 cut(s) 922
MspI CCGG 6 cut(s) 20, 44, 80, 113, 145, 187
MspR9I CCNGG 6 cut(s) 8, 145, 146, 592, 1521, 1620
MunI CAATTG 1 cut(s) 105
Mva1269I GAATGC 3 cut(s) 1631, 1820, 1890
MvaI CCWGG 4 cut(s) 8, 592, 1521, 1620
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 3 cut(s) 76, 137, 1053
NaeI GCCGGC 1 cut(s) 81
NciI CCSGG 2 cut(s) 145, 146
NcoI CCATGG 1 cut(s) 1286
NdeI CATATG 1 cut(s) 646
NdeII GATC 5 cut(s) 94, 535, 553, 958, 1724
NgoMIV GCCGGC 1 cut(s) 79
NlaIV GGNNCC 4 cut(s) 24, 125, 1405, 1674
NmeAIII GCCGAG 1 cut(s) 157
NmuCI GTSAC 1 cut(s) 1275
NsbI TGCGCA 1 cut(s) 330
NsiI ATGCAT 2 cut(s) 458, 1892
NspI RCATGY 2 cut(s) 889, 1388
PciI ACATGT 2 cut(s) 885, 1384
PcsI WCGNNNNNNNCGW 1 cut(s) 23
PctI GAATGC 3 cut(s) 1631, 1820, 1890
PdiI GCCGGC 1 cut(s) 81
PfeI GAWTC 2 cut(s) 163, 1060
PflMI CCANNNNNTGG 1 cut(s) 833
PkrI GCNGC 6 cut(s) 72, 178, 397, 572, 1543, 1597
PleI GAGTC 6 cut(s) 124, 175, 615, 1615, 1726, 1799
PpsI GAGTC 6 cut(s) 124, 175, 615, 1615, 1726, 1799
PscI ACATGT 2 cut(s) 885, 1384
PshAI GACNNNNGTC 1 cut(s) 8
PshBI ATTAAT 1 cut(s) 1344
Psp124BI GAGCTC 2 cut(s) 1045, 1641
Psp6I CCWGG 4 cut(s) 6, 590, 1519, 1618
PspFI CCCAGC 1 cut(s) 1332
PspGI CCWGG 4 cut(s) 6, 590, 1519, 1618
PspN4I GGNNCC 4 cut(s) 24, 125, 1405, 1674
PspOMI GGGCCC 1 cut(s) 123
PspPI GGNCC 7 cut(s) 110, 123, 124, 138, 704, 715, 782
PsrI GAACNNNNNNTAC 2 cut(s) 342, 374
PstI CTGCAG 1 cut(s) 1597
PstNI CAGNNNCTG 1 cut(s) 1649
PsuI RGATCY 1 cut(s) 1724
PvuII CAGCTG 1 cut(s) 1544
RsaI GTAC 4 cut(s) 825, 849, 1466, 1755
RsaNI GTAC 4 cut(s) 824, 848, 1465, 1754
RseI CAYNNNNRTG 4 cut(s) 360, 907, 1398, 1686
SacI GAGCTC 2 cut(s) 1045, 1641
SatI GCNGC 6 cut(s) 71, 177, 396, 571, 1542, 1596
Sau3AI GATC 5 cut(s) 94, 535, 553, 958, 1724
Sau96I GGNCC 7 cut(s) 110, 123, 124, 138, 704, 715, 782
SchI GAGTC 6 cut(s) 125, 175, 616, 1616, 1727, 1800
ScrFI CCNGG 6 cut(s) 8, 145, 146, 592, 1521, 1620
SduI GDGCHC 5 cut(s) 127, 625, 1045, 1558, 1641
SfaNI GCATC 2 cut(s) 421, 952
SfcI CTRYAG 3 cut(s) 721, 1325, 1593
SinI GGWCC 2 cut(s) 715, 782
SmaI CCCGGG 1 cut(s) 146
SmiMI CAYNNNNRTG 4 cut(s) 360, 907, 1398, 1686
SmlI CTYRAG 3 cut(s) 335, 922, 1602
SmoI CTYRAG 3 cut(s) 335, 922, 1602
Sse9I AATT 9 cut(s) 47, 105, 636, 746, 1297, 1345, 1489, 1579, 1665
SsiI CCGC 5 cut(s) 31, 71, 136, 176, 1853
SspI AATATT 2 cut(s) 764, 1342
SspMI CTAG 3 cut(s) 698, 1052, 1722
SstI GAGCTC 2 cut(s) 1045, 1641
StyD4I CCNGG 6 cut(s) 6, 143, 144, 590, 1519, 1618
StyI CCWWGG 3 cut(s) 982, 1286, 1373
TaaI ACNGT 9 cut(s) 156, 313, 715, 878, 1153, 1196, 1329, 1775, 1782
TaiI ACGT 2 cut(s) 240, 1466
TaqI TCGA 4 cut(s) 957, 1015, 1794, 1864
TasI AATT 9 cut(s) 47, 105, 636, 746, 1297, 1345, 1489, 1579, 1665
TatI WGTACW 1 cut(s) 847
TauI GCSGC 2 cut(s) 73, 179
TfiI GAWTC 2 cut(s) 163, 1060
TscAI CASTG 2 cut(s) 325, 331
TseFI GTSAC 1 cut(s) 1275
TseI GCWGC 4 cut(s) 395, 570, 1541, 1595
Tsp45I GTSAC 1 cut(s) 1275
TspDTI ATGAA 9 cut(s) 350, 405, 677, 1107, 1146, 1290, 1698, 1812, 1851
TspGWI ACGGA 1 cut(s) 87
TspMI CCCGGG 1 cut(s) 144
TspRI CASTG 2 cut(s) 325, 331
Van91I CCANNNNNTGG 1 cut(s) 833
Vha464I CTTAAG 1 cut(s) 922
VpaK11BI GGWCC 2 cut(s) 715, 782
VspI ATTAAT 1 cut(s) 1344
XapI RAATTY 3 cut(s) 47, 746, 1297
XbaI TCTAGA 2 cut(s) 697, 1721
XceI RCATGY 2 cut(s) 889, 1388
XcmI CCANNNNNNNNNTGG 2 cut(s) 1665, 1765
XmaI CCCGGG 1 cut(s) 144
XmiI GTMKAC 1 cut(s) 882
XspI CTAG 3 cut(s) 698, 1052, 1722
Zsp2I ATGCAT 2 cut(s) 458, 1892
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.