Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0001135

Physical Location & Seq

Genomic Coordinates Reverse (-)

7067507 .. 7074785

Gene Structure 7279 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000026928

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1941 bp

ATGATATTTCAATTGCTATTCATCGACTCAAAATTCCCTTGGATCACAAAGTTTTGGGTTCTAGAACTGTGCATCAGTCACTTACTAAAATCCTCACTTGGAACTGCCTGTGGATTCCTTTGGTATATTCAACAACTCAAAGTTTCATCTTTTGTGCTGATGGAAGATTGCTCTTCTTCTGAAATATCAATGGATAAATGGTTGACAGCTGAGCATAGGGTCCAAGAGATCTTATGGGCTGTTCAACCGAATGTAGCCTCTGAGTGGAGGAGGAGGGAGCTAATCGATTATGTTCAAAGGATAATCAAAGGTCATTTCGGAACTGAGGTGTTGTCCTTTGGCTCAGTTCCTTTAAAAACGTATCTCCCTGATGGAGATATTGATTTGACAGTACTATGTCATTGGAACCAGGAGGAAAACTTGGCTAGGGCTATCTGCAGTCGCCTTCAAGGTGAACCAAAGGAACCTGGGTTTCTGGTTGAAGATGTTAGATACGTTTATGCAAAGGTTAAGATCATAAAATGCACTGTGAATAATATAGCAGTTGATATTTCATTTAATCAGGTGGCTGGGCTGTGTACACTGTGCTTTCTGGAGCAGGTGGACCAGGTCATTGGAAGAGACCACCTTTTTAAAAAGAGTATAATCTTGATCAAAGCTTGGTGCTATTATGAGAGCCGAACTGTTGGTGCACCCCATGGCTTGATTTCAACATATGCCCTAGAAACACTGGTCTTGTATATCATCAATCTCTTTCATTCATCACTCCATGGACCTCTACAGGTTCTTCATATATTTTTGGAATACTATAGCAAATTTGATTGGACAAATTATTGCATCAGTATAAATGGTCCAGTTTCCTTATCATCCGTTCTTAAAATCAGAGAGACACCAGAAAATGAGGGGGAGAATTTACTGTTCACCAAGGAATTTTTGAAGAAATGTAGAGTTGCATTTTCAGATCCAAGAAGAATCAGCGAAGCTAGACTGCAGGAATTCCCAATCAAGTGCTTCAACGTAGTGGATCCTCTAAAAGACAACAACAATCTAGGCCGTAGTGTTACTTCAGGAAACTTCGCTCGCATTAAATCTGCTTTTGCATTTGGAGAAAATGAGCTCCGGAGAATTCTAGCGCTACCGGGTGAAAGCATTGGCCAAAAACTTCAAAAAGATTTTTTTCCCGTCACTTTAAATAGGAATGGGAGGGGACAAAGACCAGATTTGCAAGCTCCTGTACCAGCATTTGGTAGTGGAAGATATGTACAATCTAACCTCACTGGGGACTTTGATTATTATTATGGGGGAATATTGTATGCGCAGTGGCTTCATGATGAAAGCTTACATCTTTCTTCACACCCAAGTCCGCCACCTTCTCAAGTCCAGAATAACACCACATGGAATGCCTGGACTTGGTCAATGAATGTCTTTGATCAAATGTCAATGAATGCATATGCATTACCTTTCTATCATCATTGTGCTTCCCAACAGACTGCACATATAAGGACATTGACAGGAACTGGCATGTACATACCTGATCCGAGTCCTCATTACAGACAGATGTATTCAAGGGCGAGGGGAAGGAACGAGTCTAAAAATCGTCGGGTGATGATGAAACCACCCCAAAAGGTTAACCAGTCAGAAACAAGTACTGGTGTACATGTAAGCAGCATTGATCTTTCACTTGAGGAAGTTCCACATCTTCCAGGCATTCAACCGCCCCCACCTCCACAAGTCTCTCAATCTGGTAAGCCCTGGCCATCTCTTCAATCCCAGGTGCCCCCTCAGACCAATGAGGATCATAAGCTTGGGGTTTTGGGGCCTGCATCATTGCCTTTGGGAATGCATTCACTTGAAGCAAAGGCAGATTCTTGTAGTTCATATGTTAGCACCGAAGGTCCCTGTAATGGGAATGCCAAGTCAACAAGTACGTTCAAGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NTP_transf_2 PAP_assoc

Protein Analysis

647

Amino Acids

73.11

Weight (kDa)

7.86

Isoelectric Point (pI)

48.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTPAP-like_central	PF22600	74 - 192	1.8e-11	Poly(A) RNA polymerase, mitochondrial-like, central palm domain
PAP-OAS1	PF26180	205 - 396	3.4e-84	PAP/OAS1 substrate-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001135)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40520 AT2G40520 AT2G40520 AT2G40520 AT2G40520 AT2G40520 AT3G56320 AT3G56320 AT3G56320 AT3G56320 AT3G56320 AT3G56320 AT3G56320
fragaria_vesca	FvH4_3g40330 FvH4_3g40330 FvH4_3g40330 FvH4_7g25750 FvH4_7g25750 FvH4_7g25750 FvH4_7g25750
malus_domestica	MD01G1165200.v1.1 MD03G1053200.v1.1 MD07G1232500.v1.1 MD11G1054900.v1.1
prunus_persica	Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.6G042500_v2.0.a1 Prupe.6G042500_v2.0.a1
pyrus_communis	pycom01g17910 pycom03g04230 pycom11g04480
rosa_chinensis	RchiOBHm_Chr1g0372011 RchiOBHm_Chr5g0072681
rosa_laevigata	RLG00000026928 RLG00000036325
rosa_multiflora	Rmu_sc0001755.1_g000036 Rmu_sc0003160.1_g000034
rosa_roxburghii	Rroxscaffold_1G00008460
rosa_rugosa	Rorug01G0367900 Rorug01G0368000 Rorug01G0368100 Rorug01G0368200 Rorug01G0368300 Rorug05G0419200 Rorug05G0419300
rosa_samantha	Rh1AG377600 Rh1BG341000 Rh1CG354200 Rh1DG372000 Rh5AG476200 Rh5BG496400 Rh5CG520200 Rh5DG508800
rosa_wichuraiana	Rw1G033220 Rw5G044220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	589
Acc16I	TGCGCA	1 cut(s)	1317
Acc36I	ACCTGC	1 cut(s)	589
AccB1I	GGYRCC	1 cut(s)	1774
AccB7I	CCANNNNNTGG	1 cut(s)	1244
AccIII	TCCGGA	1 cut(s)	1119
AciI	CCGC	2 cut(s)	1364, 1715
AclWI	GGATC	6 cut(s)	50, 956, 1019, 1032, 1529, 1803
AcoI	YGGCCR	2 cut(s)	1153, 1754
AcsI	RAATTY	6 cut(s)	32, 815, 910, 929, 995, 1125
AcuI	CTGAAG	1 cut(s)	1050
AfaI	GTAC	8 cut(s)	393, 580, 1236, 1263, 1526, 1648, 1656, 1927
AfeI	AGCGCT	1 cut(s)	1134
AfiI	CCNNNNNNNGG	6 cut(s)	264, 1244, 1279, 1410, 1904, 1905
AflIII	ACRYGT	1 cut(s)	1657
AjnI	CCWGG	7 cut(s)	408, 466, 606, 1403, 1702, 1751, 1770
AluBI	AGCT	8 cut(s)	209, 280, 659, 983, 1117, 1229, 1338, 1804
AluI	AGCT	8 cut(s)	209, 280, 659, 983, 1117, 1229, 1338, 1804
Alw21I	GWGCWC	2 cut(s)	694, 1119
Alw26I	GTCTC	3 cut(s)	615, 881, 1738
Alw44I	GTGCAC	1 cut(s)	690
AlwI	GGATC	6 cut(s)	50, 956, 1019, 1032, 1529, 1803
AlwNI	CAGNNNCTG	1 cut(s)	1517
Aor13HI	TCCGGA	1 cut(s)	1119
Aor51HI	AGCGCT	1 cut(s)	1134
AoxI	GGCC	4 cut(s)	1051, 1153, 1754, 1817
ApaLI	GTGCAC	1 cut(s)	690
ApeKI	GCWGC	1 cut(s)	1665
ApoI	RAATTY	6 cut(s)	32, 815, 910, 929, 995, 1125
Asp700I	GAANNNNTTC	1 cut(s)	1843
AspLEI	GCGC	2 cut(s)	1135, 1318
AspS9I	GGNCC	6 cut(s)	220, 604, 773, 851, 1817, 1895
AsuC2I	CCSGG	1 cut(s)	1140
AsuHPI	GGTGA	4 cut(s)	464, 913, 1154, 1615
AvaII	GGWCC	5 cut(s)	220, 604, 773, 851, 1895
BaeGI	GKGCMC	2 cut(s)	694, 1779
BalI	TGGCCA	2 cut(s)	1155, 1756
BamHI	GGATCC	1 cut(s)	1024
BanI	GGYRCC	1 cut(s)	1774
BanII	GRGCYC	1 cut(s)	1119
BarI	GAAGNNNNNNTAC	2 cut(s)	1048, 1080
Bbv12I	GWGCWC	2 cut(s)	694, 1119
BbvI	GCAGC	1 cut(s)	1677
BccI	CCATC	3 cut(s)	154, 365, 1765
BceAI	ACGGC	1 cut(s)	1038
BciT130I	CCWGG	7 cut(s)	410, 468, 608, 1405, 1704, 1753, 1772
BclI	TGATCA	2 cut(s)	651, 1429
BcnI	CCSGG	1 cut(s)	1140
BcoDI	GTCTC	3 cut(s)	615, 881, 1738
BfaI	CTAG	6 cut(s)	62, 426, 722, 984, 1049, 1130
BfmI	CTRYAG	4 cut(s)	436, 779, 808, 989
BfoI	RGCGCY	1 cut(s)	1136
BfuAI	ACCTGC	1 cut(s)	589
BglII	AGATCT	1 cut(s)	228
BisI	GCNGC	1 cut(s)	1666
BlpI	GCTNAGC	1 cut(s)	210
BlsI	GCNGC	1 cut(s)	1667
BmcAI	AGTACT	2 cut(s)	393, 1648
Bme1390I	CCNGG	8 cut(s)	410, 468, 608, 1140, 1405, 1704, 1753, 1772
Bme18I	GGWCC	5 cut(s)	220, 604, 773, 851, 1895
BmgT120I	GGNCC	6 cut(s)	220, 604, 773, 851, 1817, 1895
BmiI	GGNNCC	7 cut(s)	221, 407, 465, 1026, 1776, 1818, 1897
BmrFI	CCNGG	8 cut(s)	410, 468, 608, 1140, 1405, 1704, 1753, 1772
BmrI	ACTGGG	1 cut(s)	1287
BmsI	GCATC	3 cut(s)	81, 846, 1832
BmuI	ACTGGG	1 cut(s)	1287
BpmI	CTGGAG	1 cut(s)	614
Bpu1102I	GCTNAGC	1 cut(s)	210
BpuEI	CTTGAG	2 cut(s)	1359, 1703
BpuMI	CCSGG	1 cut(s)	1140
Bsa29I	ATCGAT	1 cut(s)	285
BsaI	GGTCTC	1 cut(s)	615
BsaJI	CCNNGG	7 cut(s)	38, 467, 697, 769, 924, 1751, 1770
BsaWI	WCCGGW	1 cut(s)	1119
Bsc4I	CCNNNNNNNGG	6 cut(s)	264, 1244, 1279, 1410, 1904, 1905
Bse1I	ACTGG	6 cut(s)	735, 854, 1282, 1522, 1633, 1654
Bse3DI	GCAATG	1 cut(s)	1826
BseAI	TCCGGA	1 cut(s)	1119
BseBI	CCWGG	7 cut(s)	410, 468, 608, 1405, 1704, 1753, 1772
BseCI	ATCGAT	1 cut(s)	285
BseDI	CCNNGG	7 cut(s)	38, 467, 697, 769, 924, 1751, 1770
BseGI	GGATG	1 cut(s)	866
BseLI	CCNNNNNNNGG	6 cut(s)	264, 1244, 1279, 1410, 1904, 1905
BseMI	GCAATG	1 cut(s)	1826
BseMII	CTCAG	5 cut(s)	201, 252, 315, 357, 1796
BseNI	ACTGG	6 cut(s)	735, 854, 1282, 1522, 1633, 1654
BseRI	GAGGAG	2 cut(s)	283, 286
BseSI	GKGCMC	2 cut(s)	694, 1779
BseXI	GCAGC	1 cut(s)	1677
BseYI	CCCAGC	1 cut(s)	569
BsgI	GTGCAG	1 cut(s)	1476
BshFI	GGCC	4 cut(s)	1053, 1155, 1756, 1819
BshNI	GGYRCC	1 cut(s)	1774
BshVI	ATCGAT	1 cut(s)	285
BsiHKAI	GWGCWC	2 cut(s)	694, 1119
BsiSI	CCGG	2 cut(s)	1120, 1139
BslFI	GGGAC	3 cut(s)	1221, 1295, 1881
BslI	CCNNNNNNNGG	6 cut(s)	264, 1244, 1279, 1410, 1904, 1905
BsmAI	GTCTC	3 cut(s)	615, 881, 1738
BsmFI	GGGAC	3 cut(s)	1221, 1295, 1881
BsmI	GAATGC	6 cut(s)	1405, 1450, 1707, 1843, 1845, 1915
BsnI	GGCC	4 cut(s)	1053, 1155, 1756, 1819
Bso31I	GGTCTC	1 cut(s)	615
Bsp1286I	GDGCHC	3 cut(s)	694, 1119, 1779
Bsp13I	TCCGGA	1 cut(s)	1119
Bsp1407I	TGTACA	4 cut(s)	578, 1261, 1524, 1654
Bsp1720I	GCTNAGC	1 cut(s)	210
Bsp19I	CCATGG	2 cut(s)	697, 769
BspACI	CCGC	2 cut(s)	1364, 1715
BspANI	GGCC	4 cut(s)	1053, 1155, 1756, 1819
BspCNI	CTCAG	5 cut(s)	202, 253, 316, 356, 1795
BspDI	ATCGAT	1 cut(s)	285
BspEI	TCCGGA	1 cut(s)	1119
BspHI	TCATGA	1 cut(s)	1327
BspLI	GGNNCC	7 cut(s)	221, 407, 465, 1026, 1776, 1818, 1897
BspMAI	CTGCAG	2 cut(s)	440, 993
BspMI	ACCTGC	1 cut(s)	589
BspPI	GGATC	6 cut(s)	50, 956, 1019, 1032, 1529, 1803
BspQI	GCTCTTC	1 cut(s)	178
BspT107I	GGYRCC	1 cut(s)	1774
BspTNI	GGTCTC	1 cut(s)	615
BsrDI	GCAATG	1 cut(s)	1826
BsrGI	TGTACA	4 cut(s)	578, 1261, 1524, 1654
BsrI	ACTGG	6 cut(s)	735, 854, 1282, 1522, 1633, 1654
BssECI	CCNNGG	7 cut(s)	38, 467, 697, 769, 924, 1751, 1770
BssT1I	CCWWGG	4 cut(s)	38, 697, 769, 924
Bst2UI	CCWGG	7 cut(s)	410, 468, 608, 1405, 1704, 1753, 1772
Bst4CI	ACNGT	6 cut(s)	69, 391, 529, 585, 685, 918
Bst6I	CTCTTC	3 cut(s)	178, 613, 1767
BstAUI	TGTACA	4 cut(s)	578, 1261, 1524, 1654
BstC8I	GCNNGC	3 cut(s)	1081, 1227, 1821
BstDEI	CTNAG	5 cut(s)	210, 261, 324, 343, 1782
BstDSI	CCRYGG	2 cut(s)	697, 769
BstF5I	GGATG	1 cut(s)	866
BstH2I	RGCGCY	1 cut(s)	1136
BstHHI	GCGC	2 cut(s)	1135, 1318
BstMAI	GTCTC	3 cut(s)	615, 881, 1738
BstNI	CCWGG	7 cut(s)	410, 468, 608, 1405, 1704, 1753, 1772
BstNSI	RCATGY	2 cut(s)	1525, 1661
BstSCI	CCNGG	8 cut(s)	408, 466, 606, 1138, 1403, 1702, 1751, 1770
BstSFI	CTRYAG	4 cut(s)	436, 779, 808, 989
BstSLI	GKGCMC	2 cut(s)	694, 1779
BstV1I	GCAGC	1 cut(s)	1677
BstX2I	RGATCY	3 cut(s)	228, 961, 1024
BstXI	CCANNNNNNTGG	1 cut(s)	614
BstYI	RGATCY	3 cut(s)	228, 961, 1024
Bsu15I	ATCGAT	1 cut(s)	285
BsuRI	GGCC	4 cut(s)	1053, 1155, 1756, 1819
BsuTUI	ATCGAT	1 cut(s)	285
BtgI	CCRYGG	2 cut(s)	697, 769
BtsCI	GGATG	1 cut(s)	866
BtsI	GCAGTG	1 cut(s)	1325
BtsIMutI	CAGTG	5 cut(s)	525, 581, 728, 1275, 1325
BveI	ACCTGC	1 cut(s)	589
Cac8I	GCNNGC	3 cut(s)	1081, 1227, 1821
CaiI	CAGNNNCTG	1 cut(s)	1517
CciI	TCATGA	1 cut(s)	1327
CfoI	GCGC	2 cut(s)	1135, 1318
Cfr13I	GGNCC	6 cut(s)	220, 604, 773, 851, 1817, 1895
ClaI	ATCGAT	1 cut(s)	285
CsiI	ACCWGGT	1 cut(s)	606
Csp6I	GTAC	8 cut(s)	392, 579, 1235, 1262, 1525, 1647, 1655, 1926
CviAII	CATG	6 cut(s)	698, 770, 1328, 1395, 1522, 1658
CviQI	GTAC	8 cut(s)	392, 579, 1235, 1262, 1525, 1647, 1655, 1926
DdeI	CTNAG	5 cut(s)	210, 261, 324, 343, 1782
DraI	TTTAAA	3 cut(s)	354, 634, 1191
EaeI	YGGCCR	2 cut(s)	1153, 1754
Eam1104I	CTCTTC	3 cut(s)	178, 613, 1767
EarI	CTCTTC	3 cut(s)	178, 613, 1767
EciI	GGCGGA	1 cut(s)	1353
Ecl136II	GAGCTC	1 cut(s)	1117
Eco130I	CCWWGG	4 cut(s)	38, 697, 769, 924
Eco24I	GRGCYC	1 cut(s)	1119
Eco31I	GGTCTC	1 cut(s)	615
Eco47I	GGWCC	5 cut(s)	220, 604, 773, 851, 1895
Eco47III	AGCGCT	1 cut(s)	1134
Eco53kI	GAGCTC	1 cut(s)	1117
Eco57I	CTGAAG	1 cut(s)	1050
EcoICRI	GAGCTC	1 cut(s)	1117
EcoO109I	RGGNCCY	2 cut(s)	1817, 1895
EcoRI	GAATTC	2 cut(s)	995, 1125
EcoRII	CCWGG	7 cut(s)	408, 466, 606, 1403, 1702, 1751, 1770
EcoT14I	CCWWGG	4 cut(s)	38, 697, 769, 924
EcoT22I	ATGCAT	3 cut(s)	1450, 1456, 1845
EcoT38I	GRGCYC	1 cut(s)	1119
ErhI	CCWWGG	4 cut(s)	38, 697, 769, 924
FaeI	CATG	6 cut(s)	701, 773, 1331, 1398, 1525, 1661
FaqI	GGGAC	3 cut(s)	1221, 1295, 1881
FatI	CATG	6 cut(s)	697, 769, 1327, 1394, 1521, 1657
FauNDI	CATATG	3 cut(s)	715, 1450, 1879
FbaI	TGATCA	2 cut(s)	651, 1429
Fnu4HI	GCNGC	1 cut(s)	1666
FokI	GGATG	1 cut(s)	853
FriOI	GRGCYC	1 cut(s)	1119
Fsp4HI	GCNGC	1 cut(s)	1666
FspBI	CTAG	6 cut(s)	62, 426, 722, 984, 1049, 1130
FspI	TGCGCA	1 cut(s)	1317
GlaI	GCGC	2 cut(s)	1134, 1317
GluI	GCNGC	1 cut(s)	1666
GsaI	CCCAGC	1 cut(s)	573
GsuI	CTGGAG	1 cut(s)	614
HaeII	RGCGCY	1 cut(s)	1136
HaeIII	GGCC	4 cut(s)	1053, 1155, 1756, 1819
HapII	CCGG	2 cut(s)	1120, 1139
HhaI	GCGC	2 cut(s)	1135, 1318
Hin1II	CATG	6 cut(s)	701, 773, 1331, 1398, 1525, 1661
Hin6I	GCGC	2 cut(s)	1133, 1316
HinP1I	GCGC	2 cut(s)	1133, 1316
HincII	GTYRAC	3 cut(s)	204, 1630, 1920
HindII	GTYRAC	3 cut(s)	204, 1630, 1920
HindIII	AAGCTT	3 cut(s)	657, 1336, 1802
HinfI	GANTC	6 cut(s)	26, 114, 972, 1540, 1586, 1865
HpaI	GTTAAC	1 cut(s)	1630
HpaII	CCGG	2 cut(s)	1120, 1139
HphI	GGTGA	4 cut(s)	464, 913, 1154, 1615
Hpy188I	TCNGA	8 cut(s)	181, 262, 320, 884, 961, 1539, 1639, 1785
Hpy188III	TCNNGA	7 cut(s)	62, 593, 649, 1068, 1120, 1328, 1381
Hpy99I	CGWCG	1 cut(s)	1602
HpyAV	CCTTC	4 cut(s)	455, 1380, 1572, 1886
HpyCH4III	ACNGT	6 cut(s)	69, 391, 529, 585, 685, 918
HpyCH4IV	ACGT	4 cut(s)	359, 495, 1017, 1928
HpyF3I	CTNAG	5 cut(s)	210, 261, 324, 343, 1782
HpySE526I	ACGT	4 cut(s)	359, 495, 1017, 1928
Hsp92II	CATG	6 cut(s)	701, 773, 1331, 1398, 1525, 1661
HspAI	GCGC	2 cut(s)	1133, 1316
Kpn2I	TCCGGA	1 cut(s)	1119
Ksp22I	TGATCA	2 cut(s)	651, 1429
KspAI	GTTAAC	1 cut(s)	1630
LguI	GCTCTTC	1 cut(s)	178
LmnI	GCTCC	4 cut(s)	277, 595, 1122, 1234
Lsp1109I	GCAGC	1 cut(s)	1677
LweI	GCATC	3 cut(s)	81, 846, 1832
MabI	ACCWGGT	1 cut(s)	606
MaeI	CTAG	6 cut(s)	62, 426, 722, 984, 1049, 1130
MaeII	ACGT	4 cut(s)	359, 495, 1017, 1928
MaeIII	GTNAC	3 cut(s)	77, 1060, 1183
MfeI	CAATTG	1 cut(s)	11
MflI	RGATCY	3 cut(s)	228, 961, 1024
MhlI	GDGCHC	3 cut(s)	694, 1119, 1779
MlsI	TGGCCA	2 cut(s)	1155, 1756
MluCI	AATT	8 cut(s)	11, 32, 815, 829, 910, 929, 995, 1125
MluNI	TGGCCA	2 cut(s)	1155, 1756
MlyI	GAGTC	3 cut(s)	20, 1549, 1595
Mox20I	TGGCCA	2 cut(s)	1155, 1756
Mph1103I	ATGCAT	3 cut(s)	1450, 1456, 1845
MroI	TCCGGA	1 cut(s)	1119
MroXI	GAANNNNTTC	1 cut(s)	1843
MscI	TGGCCA	2 cut(s)	1155, 1756
MseI	TTAA	8 cut(s)	353, 510, 558, 633, 876, 1086, 1190, 1629
MslI	CAYNNNNRTG	1 cut(s)	1473
Msp20I	TGGCCA	2 cut(s)	1155, 1756
MspA1I	CMGCKG	1 cut(s)	209
MspI	CCGG	2 cut(s)	1120, 1139
MspR9I	CCNGG	8 cut(s)	410, 468, 608, 1140, 1405, 1704, 1753, 1772
MunI	CAATTG	1 cut(s)	11
Mva1269I	GAATGC	6 cut(s)	1405, 1450, 1707, 1843, 1845, 1915
MvaI	CCWGG	7 cut(s)	410, 468, 608, 1405, 1704, 1753, 1772
NciI	CCSGG	1 cut(s)	1140
NcoI	CCATGG	2 cut(s)	697, 769
NdeI	CATATG	3 cut(s)	715, 1450, 1879
NlaIII	CATG	6 cut(s)	701, 773, 1331, 1398, 1525, 1661
NlaIV	GGNNCC	7 cut(s)	221, 407, 465, 1026, 1776, 1818, 1897
NmuCI	GTSAC	2 cut(s)	77, 1183
NsbI	TGCGCA	1 cut(s)	1317
NsiI	ATGCAT	3 cut(s)	1450, 1456, 1845
NspI	RCATGY	2 cut(s)	1525, 1661
PagI	TCATGA	1 cut(s)	1327
PaqCI	CACCTGC	1 cut(s)	589
PciI	ACATGT	1 cut(s)	1657
PciSI	GCTCTTC	1 cut(s)	178
PctI	GAATGC	6 cut(s)	1405, 1450, 1707, 1843, 1845, 1915
PdmI	GAANNNNTTC	1 cut(s)	1843
PfeI	GAWTC	3 cut(s)	114, 972, 1865
PflFI	GACNNNGTC	2 cut(s)	608, 1411
PflMI	CCANNNNNTGG	1 cut(s)	1244
PkrI	GCNGC	1 cut(s)	1667
PleI	GAGTC	3 cut(s)	20, 1548, 1594
PpsI	GAGTC	3 cut(s)	20, 1548, 1594
PpuMI	RGGWCCY	1 cut(s)	1895
PscI	ACATGT	1 cut(s)	1657
Psp124BI	GAGCTC	1 cut(s)	1119
Psp5II	RGGWCCY	1 cut(s)	1895
Psp6I	CCWGG	7 cut(s)	408, 466, 606, 1403, 1702, 1751, 1770
PspFI	CCCAGC	1 cut(s)	569
PspGI	CCWGG	7 cut(s)	408, 466, 606, 1403, 1702, 1751, 1770
PspN4I	GGNNCC	7 cut(s)	221, 407, 465, 1026, 1776, 1818, 1897
PspPI	GGNCC	6 cut(s)	220, 604, 773, 851, 1817, 1895
PspPPI	RGGWCCY	1 cut(s)	1895
PstI	CTGCAG	2 cut(s)	440, 993
PstNI	CAGNNNCTG	1 cut(s)	1517
PsuI	RGATCY	3 cut(s)	228, 961, 1024
PsyI	GACNNNGTC	2 cut(s)	608, 1411
PvuII	CAGCTG	1 cut(s)	209
RsaI	GTAC	8 cut(s)	393, 580, 1236, 1263, 1526, 1648, 1656, 1927
RsaNI	GTAC	8 cut(s)	392, 579, 1235, 1262, 1525, 1647, 1655, 1926
RseI	CAYNNNNRTG	1 cut(s)	1473
SacI	GAGCTC	1 cut(s)	1119
SapI	GCTCTTC	1 cut(s)	178
SaqAI	TTAA	8 cut(s)	353, 510, 558, 633, 876, 1086, 1190, 1629
SatI	GCNGC	1 cut(s)	1666
Sau96I	GGNCC	6 cut(s)	220, 604, 773, 851, 1817, 1895
ScaI	AGTACT	2 cut(s)	393, 1648
SchI	GAGTC	3 cut(s)	20, 1549, 1595
ScrFI	CCNGG	8 cut(s)	410, 468, 608, 1140, 1405, 1704, 1753, 1772
SduI	GDGCHC	3 cut(s)	694, 1119, 1779
SexAI	ACCWGGT	1 cut(s)	606
SfaNI	GCATC	3 cut(s)	81, 846, 1832
SfcI	CTRYAG	4 cut(s)	436, 779, 808, 989
SinI	GGWCC	5 cut(s)	220, 604, 773, 851, 1895
SmiMI	CAYNNNNRTG	1 cut(s)	1473
SmlI	CTYRAG	2 cut(s)	1374, 1682
SmoI	CTYRAG	2 cut(s)	1374, 1682
Sse9I	AATT	8 cut(s)	11, 32, 815, 829, 910, 929, 995, 1125
SsiI	CCGC	2 cut(s)	1364, 1715
SspI	AATATT	1 cut(s)	1308
SspMI	CTAG	6 cut(s)	62, 426, 722, 984, 1049, 1130
SstI	GAGCTC	1 cut(s)	1119
StyD4I	CCNGG	8 cut(s)	408, 466, 606, 1138, 1403, 1702, 1751, 1770
StyI	CCWWGG	4 cut(s)	38, 697, 769, 924
TaaI	ACNGT	6 cut(s)	69, 391, 529, 585, 685, 918
TaiI	ACGT	4 cut(s)	362, 498, 1020, 1931
TaqI	TCGA	2 cut(s)	24, 285
TasI	AATT	8 cut(s)	11, 32, 815, 829, 910, 929, 995, 1125
TatI	WGTACW	6 cut(s)	391, 578, 1261, 1524, 1646, 1654
TfiI	GAWTC	3 cut(s)	114, 972, 1865
Tru1I	TTAA	8 cut(s)	353, 510, 558, 633, 876, 1086, 1190, 1629
Tru9I	TTAA	8 cut(s)	353, 510, 558, 633, 876, 1086, 1190, 1629
TscAI	CASTG	5 cut(s)	532, 588, 735, 1282, 1325
TseFI	GTSAC	2 cut(s)	77, 1183
TseI	GCWGC	1 cut(s)	1665
Tsp45I	GTSAC	2 cut(s)	77, 1183
TspGWI	ACGGA	1 cut(s)	859
TspRI	CASTG	5 cut(s)	532, 588, 735, 1282, 1325
Tth111I	GACNNNGTC	2 cut(s)	608, 1411
Van91I	CCANNNNNTGG	1 cut(s)	1244
VneI	GTGCAC	1 cut(s)	690
VpaK11BI	GGWCC	5 cut(s)	220, 604, 773, 851, 1895
XapI	RAATTY	6 cut(s)	32, 815, 910, 929, 995, 1125
XbaI	TCTAGA	1 cut(s)	61
XceI	RCATGY	2 cut(s)	1525, 1661
XmnI	GAANNNNTTC	1 cut(s)	1843
XspI	CTAG	6 cut(s)	62, 426, 722, 984, 1049, 1130
ZrmI	AGTACT	2 cut(s)	393, 1648
Zsp2I	ATGCAT	3 cut(s)	1450, 1456, 1845

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.