pycom03g04230

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0001135
Physical Location & Seq
Forward (+)
3327522 .. 3330750
3229 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g04230.5

Sequence Viewer

Length: 1662 bp
ATGGCCCGCCTCCTCTGTTCGGCGCGGCTGAACGACGGCGTTTTCCGCGAGGATCGACCCCGCCCTTCGCCTCCCTCGCCGTCATTGCCGACCTCCAACCCCGATCACTCCTCCAGAGCCCCTGAGTCTTGGGCCGGAGCCGAGCAGGCGACCCAGGAGATAGTCTCAAAGATTCAGCCTACTCTGGCGGCCCACCAGACGAGGACGGAGGTCATTCAATTTGTGCAGAACCTCATCACATGTAACGTCAGGTGTCAGGTTTTTCCTTATGGGTCAGTTCCATTGAAAACGTATCTGCCTGATGGAGATATTGATATGACGGCATTTGGCAGTGCAAATATTGATAAAGTCTTTGCAACTGATGTCTATGCAATTATGAAGGGAGAGGAGAACAACGAAGCTTCTCCGTTTCATGTGAAAGATGTACATTACATTGATGCAGAGGTTAAGCTTGTGAAGTGCATTATACAAAATATAGTTGTAGATATATCCTCCAATCAGTTAGGAGGATTTAGTACTCTGCACTTTCTTGAACAGGTTGATAATTATATTGGCAAAAATCATCTCTTCAAGCGGAGTATTATTCTGATAAAAGCCTGGTGCTACTATGAGAGTCGTATTCTTGGGGCCCATCATGGGTTGATTTCAACATATGCTTTGGAAATATTAATCCTATATGTTTTCCATGTTTTCCATTCATCACTGGATGGCCCTCTGGCAGTCCTTTATAGATTTTTGGATTACTTCAGCAAATTTGACTGGGAGAACTATTGTATAAGTTTAAACGGACCAGTATGCAAATCATCCTTGCCTGATATAGTGGCTAAGGCACCCGAAAGCGGGAGAGTTGATCGACTACTGAGTGAAGAATTTATTAGAAATTGTGTGGAAATGTTCTCTGTTCCAGCAAAGGGGTATGAGACAAACTTGCGAGCTTTTTCCCTGAAGCATCTTAATATGATAGATCCACTAAAGCAGGACAACAACCTTGGGCGAAGTGTCAGCAGAAGTAACTTCTATCGAATATGCAGTGCTTTTAAACATGGAGCTCGCAAGCTTGGTTGGATTCTTTCATTACCAGGGGAATGCATAGCAGATGAGCTGCCCAAGTTTTTTGTAAATACTTTGGATAGACATGGAAGCAATGGTCAGACTAATGTGCACAATAATGCCTTGTCTGGAAGTTCTGAATCTTTGAACGTTTTGTTGGAGCTTGGTGGAGATTTCGACAGTCATGGAATGAACCTGCAATATGGTCCCTTTTTCCCTGGATCTGCTCCATTACCTCCTTTATTTCCCAGTCTTCCCTTGTCACCTCAGCTGTGGAAAGAGAACCGATGGGGAGGTACGTCTGAAGCCCTTCATTTCCATCAGAATACTAATTCTCAAACTAACGTACATGGTGTTGACTGGGGAGCACATGCGTACCATGCAAATGATTCCACTTTCTCCGTTGCTGCTTTCAGAGGAGAGAAAAGGATACCCCGAGGGACTGGAACGTACATCCCAAATCCGTCTTTTCAGAATTACCGTCCCTTAAAGGATAGGTTGCCTGGAAGGGGAAGGAAACAGGCACCAGGAACTTGTTTTCCGTTTCAGAGACACGCCTTTGGTTATGGCTTCACTGTAGCTCCTCAACAGTCAATCTCACCTAGAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PAP_assoc

Protein Analysis

554

Amino Acids

61.82

Weight (kDa)

7.65

Isoelectric Point (pI)

39.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1254
AccB1I GGYRCC 2 cut(s) 829, 1573
AccII CGCG 2 cut(s) 25, 48
AciI CCGC 7 cut(s) 7, 25, 46, 61, 188, 574, 840
AclI AACGTT 1 cut(s) 1200
AclWI GGATC 3 cut(s) 60, 959, 1279
AcsI RAATTY 2 cut(s) 752, 869
AcuI CTGAAG 3 cut(s) 730, 965, 1374
AfaI GTAC 6 cut(s) 426, 517, 1348, 1398, 1427, 1502
AfiI CCNNNNNNNGG 6 cut(s) 19, 636, 839, 840, 911, 1559
AflIII ACRYGT 1 cut(s) 239
AgsI TTSAA 6 cut(s) 218, 286, 533, 571, 648, 1198
AjnI CCWGG 6 cut(s) 153, 596, 1078, 1267, 1552, 1576
AjuI GAANNNNNNNTTGG 2 cut(s) 1190, 1222
AloI GAACNNNNNNTCC 2 cut(s) 1573, 1605
AluBI AGCT 9 cut(s) 401, 451, 935, 1049, 1057, 1102, 1213, 1321, 1631
AluI AGCT 9 cut(s) 401, 451, 935, 1049, 1057, 1102, 1213, 1321, 1631
Alw21I GWGCWC 3 cut(s) 1051, 1164, 1420
Alw26I GTCTC 3 cut(s) 169, 914, 1594
Alw44I GTGCAC 1 cut(s) 1160
AlwI GGATC 3 cut(s) 60, 959, 1279
Ama87I CYCGRG 1 cut(s) 1485
AoxI GGCC 5 cut(s) 3, 132, 189, 627, 709
ApaI GGGCCC 1 cut(s) 631
ApaLI GTGCAC 1 cut(s) 1160
ApeKI GCWGC 2 cut(s) 1102, 1457
ApoI RAATTY 2 cut(s) 752, 869
AseI ATTAAT 1 cut(s) 668
Asp700I GAANNNNTTC 1 cut(s) 1359
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 8 cut(s) 4, 132, 190, 627, 628, 710, 788, 1256
AsuHPI GGTGA 2 cut(s) 1305, 1641
AvaI CYCGRG 1 cut(s) 1485
AvaII GGWCC 2 cut(s) 788, 1256
BaeGI GKGCMC 2 cut(s) 631, 1164
BaeI ACNNNNGTAYC 2 cut(s) 1409, 1442
BanI GGYRCC 2 cut(s) 829, 1573
BanII GRGCYC 3 cut(s) 121, 631, 1051
BbsI GAAGAC 1 cut(s) 1295
Bbv12I GWGCWC 3 cut(s) 1051, 1164, 1420
BbvCI CCTCAGC 1 cut(s) 1317
BbvI GCAGC 2 cut(s) 1089, 1444
BccI CCATC 5 cut(s) 296, 639, 701, 1332, 1377
BceAI ACGGC 3 cut(s) 52, 64, 336
BciT130I CCWGG 6 cut(s) 155, 598, 1080, 1269, 1554, 1578
BciVI GTATCC 1 cut(s) 1473
BcoDI GTCTC 3 cut(s) 169, 914, 1594
BfaI CTAG 1 cut(s) 1653
BfmI CTRYAG 1 cut(s) 1626
BfuAI ACCTGC 1 cut(s) 1254
BfuI GTATCC 1 cut(s) 1473
BglI GCCNNNNNGGC 1 cut(s) 146
BisI GCNGC 4 cut(s) 26, 189, 1103, 1458
BlsI GCNGC 4 cut(s) 27, 190, 1104, 1459
BmcAI AGTACT 1 cut(s) 517
Bme1390I CCNGG 6 cut(s) 155, 598, 1080, 1269, 1554, 1578
Bme18I GGWCC 2 cut(s) 788, 1256
BmeT110I CYCGRG 1 cut(s) 1485
BmgT120I GGNCC 8 cut(s) 4, 132, 190, 627, 628, 710, 788, 1256
BmiI GGNNCC 6 cut(s) 139, 628, 629, 831, 1258, 1575
BmrFI CCNGG 6 cut(s) 155, 598, 1080, 1269, 1554, 1578
BmrI ACTGGG 3 cut(s) 769, 1293, 1420
BmsI GCATC 2 cut(s) 427, 958
BmuI ACTGGG 3 cut(s) 769, 1293, 1420
BoxI GACNNNNGTC 1 cut(s) 209
BpiI GAAGAC 1 cut(s) 1295
BplI GAGNNNNNCTC 2 cut(s) 149, 181
BpmI CTGGAG 1 cut(s) 97
Bpu10I CCTNAGC 2 cut(s) 825, 1317
BsaJI CCNNGG 5 cut(s) 153, 988, 1079, 1267, 1486
BsaXI ACNNNNNCTCC 2 cut(s) 1615, 1645
Bsc4I CCNNNNNNNGG 6 cut(s) 19, 636, 839, 840, 911, 1559
Bse1I ACTGG 6 cut(s) 708, 764, 791, 1299, 1415, 1498
Bse3DI GCAATG 2 cut(s) 83, 1150
BseBI CCWGG 6 cut(s) 155, 598, 1080, 1269, 1554, 1578
BseDI CCNNGG 5 cut(s) 153, 988, 1079, 1267, 1486
BseGI GGATG 3 cut(s) 712, 803, 1503
BseLI CCNNNNNNNGG 6 cut(s) 19, 636, 839, 840, 911, 1559
BseMI GCAATG 2 cut(s) 83, 1150
BseMII CTCAG 3 cut(s) 114, 851, 1331
BseNI ACTGG 6 cut(s) 708, 764, 791, 1299, 1415, 1498
BseRI GAGGAG 4 cut(s) 100, 401, 1482, 1623
BseSI GKGCMC 2 cut(s) 631, 1164
BseXI GCAGC 2 cut(s) 1089, 1444
BsgI GTGCAG 2 cut(s) 245, 506
Bsh1236I CGCG 2 cut(s) 25, 48
BshFI GGCC 5 cut(s) 5, 134, 191, 629, 711
BshNI GGYRCC 2 cut(s) 829, 1573
BsiHKAI GWGCWC 3 cut(s) 1051, 1164, 1420
BsiHKCI CYCGRG 1 cut(s) 1485
BsiSI CCGG 1 cut(s) 135
BslFI GGGAC 3 cut(s) 1242, 1504, 1518
BslI CCNNNNNNNGG 6 cut(s) 19, 636, 839, 840, 911, 1559
BsmAI GTCTC 3 cut(s) 169, 914, 1594
BsmFI GGGAC 3 cut(s) 1242, 1504, 1518
BsmI GAATGC 1 cut(s) 1091
BsnI GGCC 5 cut(s) 5, 134, 191, 629, 711
BsoBI CYCGRG 1 cut(s) 1485
Bsp120I GGGCCC 1 cut(s) 627
Bsp1286I GDGCHC 5 cut(s) 121, 631, 1051, 1164, 1420
Bsp1407I TGTACA 1 cut(s) 424
Bsp143I GATC 5 cut(s) 52, 103, 850, 964, 1271
BspACI CCGC 7 cut(s) 7, 25, 46, 61, 188, 574, 840
BspANI GGCC 5 cut(s) 5, 134, 191, 629, 711
BspCNI CTCAG 3 cut(s) 115, 852, 1330
BspFNI CGCG 2 cut(s) 25, 48
BspLI GGNNCC 6 cut(s) 139, 628, 629, 831, 1258, 1575
BspMI ACCTGC 1 cut(s) 1254
BspPI GGATC 3 cut(s) 60, 959, 1279
BspT107I GGYRCC 2 cut(s) 829, 1573
BsrDI GCAATG 2 cut(s) 83, 1150
BsrGI TGTACA 1 cut(s) 424
BsrI ACTGG 6 cut(s) 708, 764, 791, 1299, 1415, 1498
BssECI CCNNGG 5 cut(s) 153, 988, 1079, 1267, 1486
BssMI GATC 5 cut(s) 52, 103, 850, 964, 1271
BssT1I CCWWGG 1 cut(s) 988
Bst2UI CCWGG 6 cut(s) 155, 598, 1080, 1269, 1554, 1578
Bst4CI ACNGT 4 cut(s) 1232, 1532, 1627, 1641
Bst6I CTCTTC 1 cut(s) 572
BstAUI TGTACA 1 cut(s) 424
BstC8I GCNNGC 5 cut(s) 7, 147, 933, 1051, 1055
BstDEI CTNAG 4 cut(s) 123, 825, 860, 1317
BstF5I GGATG 3 cut(s) 712, 803, 1503
BstFNI CGCG 2 cut(s) 25, 48
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 5 cut(s) 55, 106, 853, 967, 1274
BstMAI GTCTC 3 cut(s) 169, 914, 1594
BstMBI GATC 5 cut(s) 52, 103, 850, 964, 1271
BstMWI GCNNNNNNNGC 5 cut(s) 45, 76, 85, 146, 1430
BstNI CCWGG 6 cut(s) 155, 598, 1080, 1269, 1554, 1578
BstNSI RCATGY 2 cut(s) 243, 1424
BstPAI GACNNNNGTC 1 cut(s) 209
BstSCI CCNGG 6 cut(s) 153, 596, 1078, 1267, 1552, 1576
BstSFI CTRYAG 1 cut(s) 1626
BstSLI GKGCMC 2 cut(s) 631, 1164
BstUI CGCG 2 cut(s) 25, 48
BstV1I GCAGC 2 cut(s) 1089, 1444
BstV2I GAAGAC 1 cut(s) 1295
BstX2I RGATCY 2 cut(s) 964, 1271
BstYI RGATCY 2 cut(s) 964, 1271
BsuI GTATCC 1 cut(s) 1473
BsuRI GGCC 5 cut(s) 5, 134, 191, 629, 711
BtsCI GGATG 3 cut(s) 712, 803, 1503
BtsI GCAGTG 2 cut(s) 337, 1036
BtsIMutI CAGTG 4 cut(s) 337, 701, 1036, 1623
BveI ACCTGC 1 cut(s) 1254
Cac8I GCNNGC 5 cut(s) 7, 147, 933, 1051, 1055
CfoI GCGC 1 cut(s) 25
Cfr13I GGNCC 8 cut(s) 4, 132, 190, 627, 628, 710, 788, 1256
Csp6I GTAC 6 cut(s) 425, 516, 1347, 1397, 1426, 1501
CviQI GTAC 6 cut(s) 425, 516, 1347, 1397, 1426, 1501
DdeI CTNAG 4 cut(s) 123, 825, 860, 1317
DpnI GATC 5 cut(s) 54, 105, 852, 966, 1273
DpnII GATC 5 cut(s) 52, 103, 850, 964, 1271
DraI TTTAAA 2 cut(s) 783, 1039
Eam1104I CTCTTC 1 cut(s) 572
EarI CTCTTC 1 cut(s) 572
Ecl136II GAGCTC 1 cut(s) 1049
Eco130I CCWWGG 1 cut(s) 988
Eco24I GRGCYC 3 cut(s) 121, 631, 1051
Eco47I GGWCC 2 cut(s) 788, 1256
Eco53kI GAGCTC 1 cut(s) 1049
Eco57I CTGAAG 3 cut(s) 730, 965, 1374
Eco88I CYCGRG 1 cut(s) 1485
EcoICRI GAGCTC 1 cut(s) 1049
EcoO109I RGGNCCY 1 cut(s) 627
EcoRII CCWGG 6 cut(s) 153, 596, 1078, 1267, 1552, 1576
EcoT14I CCWWGG 1 cut(s) 988
EcoT22I ATGCAT 1 cut(s) 1091
EcoT38I GRGCYC 3 cut(s) 121, 631, 1051
ErhI CCWWGG 1 cut(s) 988
FaqI GGGAC 3 cut(s) 1242, 1504, 1518
FauI CCCGC 3 cut(s) 14, 68, 833
FauNDI CATATG 1 cut(s) 652
Fnu4HI GCNGC 4 cut(s) 26, 189, 1103, 1458
FokI GGATG 3 cut(s) 719, 790, 1490
FriOI GRGCYC 3 cut(s) 121, 631, 1051
Fsp4HI GCNGC 4 cut(s) 26, 189, 1103, 1458
FspBI CTAG 1 cut(s) 1653
GlaI GCGC 1 cut(s) 24
GluI GCNGC 4 cut(s) 26, 189, 1103, 1458
GsuI CTGGAG 1 cut(s) 97
HaeIII GGCC 5 cut(s) 5, 134, 191, 629, 711
HapII CCGG 1 cut(s) 135
HhaI GCGC 1 cut(s) 25
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HincII GTYRAC 1 cut(s) 1408
HindII GTYRAC 1 cut(s) 1408
HindIII AAGCTT 3 cut(s) 399, 449, 1055
HinfI GANTC 6 cut(s) 125, 172, 613, 1066, 1190, 1439
HpaII CCGG 1 cut(s) 135
HphI GGTGA 2 cut(s) 1305, 1641
Hpy166II GTNNAC 2 cut(s) 1162, 1408
Hpy188I TCNGA 8 cut(s) 588, 1152, 1189, 1354, 1374, 1466, 1524, 1599
Hpy188III TCNNGA 3 cut(s) 114, 530, 1179
Hpy8I GTNNAC 2 cut(s) 1162, 1408
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 5 cut(s) 75, 373, 1370, 1551, 1557
HpyCH4III ACNGT 4 cut(s) 1232, 1532, 1627, 1641
HpyCH4IV ACGT 6 cut(s) 246, 290, 1200, 1349, 1395, 1499
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 76, 85, 146, 1430
HpyF3I CTNAG 4 cut(s) 123, 825, 860, 1317
HpySE526I ACGT 6 cut(s) 246, 290, 1200, 1349, 1395, 1499
HspAI GCGC 1 cut(s) 23
Kzo9I GATC 5 cut(s) 52, 103, 850, 964, 1271
LmnI GCTCC 6 cut(s) 137, 1046, 1210, 1282, 1415, 1636
Lsp1109I GCAGC 2 cut(s) 1089, 1444
LweI GCATC 2 cut(s) 427, 958
MaeI CTAG 1 cut(s) 1653
MaeII ACGT 6 cut(s) 246, 290, 1200, 1349, 1395, 1499
MaeIII GTNAC 3 cut(s) 242, 1010, 1311
MalI GATC 5 cut(s) 54, 105, 852, 966, 1273
MboI GATC 5 cut(s) 52, 103, 850, 964, 1271
MboII GAAGA 3 cut(s) 559, 878, 1295
MflI RGATCY 2 cut(s) 964, 1271
MhlI GDGCHC 5 cut(s) 121, 631, 1051, 1164, 1420
MluCI AATT 8 cut(s) 218, 372, 544, 752, 869, 880, 1381, 1525
MlyI GAGTC 2 cut(s) 134, 622
MmeI TCCRAC 3 cut(s) 120, 1043, 1188
Mph1103I ATGCAT 1 cut(s) 1091
MroXI GAANNNNTTC 1 cut(s) 1359
MseI TTAA 6 cut(s) 447, 668, 782, 954, 1038, 1538
MslI CAYNNNNRTG 2 cut(s) 1167, 1434
MspA1I CMGCKG 1 cut(s) 1321
MspI CCGG 1 cut(s) 135
MspR9I CCNGG 6 cut(s) 155, 598, 1080, 1269, 1554, 1578
MssI GTTTAAAC 1 cut(s) 783
Mva1269I GAATGC 1 cut(s) 1091
MvaI CCWGG 6 cut(s) 155, 598, 1080, 1269, 1554, 1578
MvnI CGCG 2 cut(s) 25, 48
MwoI GCNNNNNNNGC 5 cut(s) 45, 76, 85, 146, 1430
NdeI CATATG 1 cut(s) 652
NdeII GATC 5 cut(s) 52, 103, 850, 964, 1271
NlaIV GGNNCC 6 cut(s) 139, 628, 629, 831, 1258, 1575
NmeAIII GCCGAG 1 cut(s) 166
NmuCI GTSAC 1 cut(s) 1311
NsiI ATGCAT 1 cut(s) 1091
NspI RCATGY 2 cut(s) 243, 1424
PciI ACATGT 1 cut(s) 239
PctI GAATGC 1 cut(s) 1091
PdmI GAANNNNTTC 1 cut(s) 1359
PfeI GAWTC 4 cut(s) 172, 1066, 1190, 1439
PkrI GCNGC 4 cut(s) 27, 190, 1104, 1459
PleI GAGTC 2 cut(s) 133, 621
PmeI GTTTAAAC 1 cut(s) 783
PpsI GAGTC 2 cut(s) 133, 621
PscI ACATGT 1 cut(s) 239
PshAI GACNNNNGTC 1 cut(s) 209
PshBI ATTAAT 1 cut(s) 668
Psp124BI GAGCTC 1 cut(s) 1051
Psp1406I AACGTT 1 cut(s) 1200
Psp6I CCWGG 6 cut(s) 153, 596, 1078, 1267, 1552, 1576
PspGI CCWGG 6 cut(s) 153, 596, 1078, 1267, 1552, 1576
PspN4I GGNNCC 6 cut(s) 139, 628, 629, 831, 1258, 1575
PspOMI GGGCCC 1 cut(s) 627
PspPI GGNCC 8 cut(s) 4, 132, 190, 627, 628, 710, 788, 1256
PsuI RGATCY 2 cut(s) 964, 1271
PvuII CAGCTG 1 cut(s) 1321
RsaI GTAC 6 cut(s) 426, 517, 1348, 1398, 1427, 1502
RsaNI GTAC 6 cut(s) 425, 516, 1347, 1397, 1426, 1501
RseI CAYNNNNRTG 2 cut(s) 1167, 1434
SacI GAGCTC 1 cut(s) 1051
SaqAI TTAA 6 cut(s) 447, 668, 782, 954, 1038, 1538
SatI GCNGC 4 cut(s) 26, 189, 1103, 1458
Sau3AI GATC 5 cut(s) 52, 103, 850, 964, 1271
Sau96I GGNCC 8 cut(s) 4, 132, 190, 627, 628, 710, 788, 1256
ScaI AGTACT 1 cut(s) 517
SchI GAGTC 2 cut(s) 134, 622
ScrFI CCNGG 6 cut(s) 155, 598, 1080, 1269, 1554, 1578
SduI GDGCHC 5 cut(s) 121, 631, 1051, 1164, 1420
SfaNI GCATC 2 cut(s) 427, 958
SfcI CTRYAG 1 cut(s) 1626
SinI GGWCC 2 cut(s) 788, 1256
SmiMI CAYNNNNRTG 2 cut(s) 1167, 1434
Sse9I AATT 8 cut(s) 218, 372, 544, 752, 869, 880, 1381, 1525
SsiI CCGC 7 cut(s) 7, 25, 46, 61, 188, 574, 840
SspI AATATT 2 cut(s) 340, 666
SspMI CTAG 1 cut(s) 1653
SstI GAGCTC 1 cut(s) 1051
StyD4I CCNGG 6 cut(s) 153, 596, 1078, 1267, 1552, 1576
StyI CCWWGG 1 cut(s) 988
TaaI ACNGT 4 cut(s) 1232, 1532, 1627, 1641
TaiI ACGT 6 cut(s) 249, 293, 1203, 1352, 1398, 1502
TaqI TCGA 4 cut(s) 55, 853, 1021, 1227
TasI AATT 8 cut(s) 218, 372, 544, 752, 869, 880, 1381, 1525
TatI WGTACW 2 cut(s) 424, 515
TauI GCSGC 2 cut(s) 28, 191
TfiI GAWTC 4 cut(s) 172, 1066, 1190, 1439
Tru1I TTAA 6 cut(s) 447, 668, 782, 954, 1038, 1538
Tru9I TTAA 6 cut(s) 447, 668, 782, 954, 1038, 1538
TscAI CASTG 4 cut(s) 337, 708, 1036, 1630
TseFI GTSAC 1 cut(s) 1311
TseI GCWGC 2 cut(s) 1102, 1457
Tsp45I GTSAC 1 cut(s) 1311
TspDTI ATGAA 6 cut(s) 392, 401, 687, 1062, 1256, 1352
TspGWI ACGGA 6 cut(s) 221, 396, 801, 1441, 1503, 1581
TspRI CASTG 4 cut(s) 337, 708, 1036, 1630
VneI GTGCAC 1 cut(s) 1160
VpaK11BI GGWCC 2 cut(s) 788, 1256
VspI ATTAAT 1 cut(s) 668
XapI RAATTY 2 cut(s) 752, 869
XceI RCATGY 2 cut(s) 243, 1424
XmnI GAANNNNTTC 1 cut(s) 1359
XspI CTAG 1 cut(s) 1653
ZrmI AGTACT 1 cut(s) 517
Zsp2I ATGCAT 1 cut(s) 1091
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.