Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1D

Orthogroup ID

OG0001135

Physical Location & Seq

Genomic Coordinates Forward (+)

59617403 .. 59623635

Gene Structure 6233 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1DG372000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1770 bp

ATGGAAGATTGCTCTTCTTCTGAAATATCAATAGATAAATGGTTGACAGCTGAGCATAGGGTCCAAGAGATCTTATGGGCTGTTCAACCGAATGTAGCCTCTGAGTGGAGGAGGAGGGAGCTAATCGATTATGTTCAAAGGATAATCAAAGGTCATTTCGGAACTGAGGTGTTGTCCTTTGGTTCAGTTCCTTTAAAAACGTATCTCCCTGATGGAGATATTGATTTGACAGTACTCTGTCATCGGAAGCAGGAGGAAGACTTAGCTAGGGCTATCTGCAGTCACCTTCAAGGTGAACCAAAGGAACCTGGGTTCTTGGTTGAAGATGTTAGATACATTTATGCAAAGGTGGACCAGGTCGTTGGAAGAGACCACCTTTTTAAAAAGAGTATAATCTTGATCAAAGCTTGGTGCTATTATGAGAGCCGAACCGTTGGTGCACCCCATGGCTTGATTTCCACATATGCACTAGAAACACTGGTCTTGTATATCATCAATCTCTTTCATTCATCACTCCGTGGACCTCTACAGGTTCTTCATAGATTTTTGGAATACTATAGCAAATTTGATTGGACAAATTATTGCATCAGTATAAATGGTCCAGTTTCCTTATCATCCGTTCTTAAAATCAGAGAGACACCAGAAAATGAGGGGGAGAATTTACTGTTCACCAAGGAATTTTTGAAGAAATGTAGAGTTGCATTTTCAGATCCAAGAAGAATCAGCGAAGCTAGACTGCAGGAATTCCCAATCAAGTGCTTCAACGTAGTGGATCCTCTAAAAGACAACAACAATCTAGGCCGTAGTGTTACTTCAGGAAACTTCGCTCGCATTAAATCTGCTTTTGCATTTGGAGAAAATGAGCTCCGGAGAATTCTAGCGCTACCGGGTGAAAGCATTGGCCAAAAACTTCAAAAAGATTTTTTTCCTGTCACTTTAAATAGGAATGGGAGGGGACAAAGACCAGATTTGCAAGCTCTTGTACCAGCATTTGGTAGTGGAAGATATGTACAATCTAACCTCACTGGGGACTTTGATTATTATTATGGGGGAATATTGTATGCGCAGTGGCTTCATGATGAAAGCTTACATCTTTCTTCACACCCAAGTCCGCCACCTTCTCAAGTCCAGAATAACACCACATGGAATGCCAGGACTTGGTCAGTGAATGTCTTTGATCAAATGTCAATGAATGCATATGCATTACCTTTCTATCATCATTGTGCTTCCCAACAGACTGCTCATATAAGGACATTGCCAGGAACTGGCATGTACATACCTGATCCGAGTCCTCATTACAGACAGATGTATTCAAGGGCGAGGGGAAGGAACGAGTCTAAAAATCTTCGGGTGATGATGAAACCACCCCAAAAGGTTAACCAGTCAGAAACAAGTACTGGTGTACATGTAACCAGCATTGATCTTTCACTTGAGGAAGTTCCACATCTTCCAGGCACTCAACCACCCCCACCTCCACAAGTCTCTAAATCTGGTAAGCCCTGGCCATCTCTTCAATCCCAGGTGCCCCCTCAGACCAATGAGTATTGTAAGCTTGGGGTTTTGGGGCCTGCATCATTGCCTTTGGGAATGCATTCACTTGAAGCAAAAGCAGATTCTTGTAGTTCATATGCTAGGGACCTTACACCGAAGGTCCCTGTAATGGGAATGCCAAGTCAACAAGTACGTTCAAGCATTGATGAGATCAATAAAGAGCCGTTCCAGTTGGACAATAAAGATTTTCCGCCTTCGGGTAGCAAAACAAATCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NTP_transf_2 PAP_assoc

Protein Analysis

589

Amino Acids

66.59

Weight (kDa)

8.19

Isoelectric Point (pI)

52.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTPAP-like_central	PF22600	21 - 117	6.1e-06	Poly(A) RNA polymerase, mitochondrial-like, central palm domain
PAP-OAS1	PF26180	121 - 312	5.9e-85	PAP/OAS1 substrate-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001135)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G40520 AT2G40520 AT2G40520 AT2G40520 AT2G40520 AT2G40520 AT3G56320 AT3G56320 AT3G56320 AT3G56320 AT3G56320 AT3G56320 AT3G56320
fragaria_vesca	FvH4_3g40330 FvH4_3g40330 FvH4_3g40330 FvH4_7g25750 FvH4_7g25750 FvH4_7g25750 FvH4_7g25750
malus_domestica	MD01G1165200.v1.1 MD03G1053200.v1.1 MD07G1232500.v1.1 MD11G1054900.v1.1
prunus_persica	Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.2G262700_v2.0.a1 Prupe.6G042500_v2.0.a1 Prupe.6G042500_v2.0.a1
pyrus_communis	pycom01g17910 pycom03g04230 pycom11g04480
rosa_chinensis	RchiOBHm_Chr1g0372011 RchiOBHm_Chr5g0072681
rosa_laevigata	RLG00000026928 RLG00000036325
rosa_multiflora	Rmu_sc0001755.1_g000036 Rmu_sc0003160.1_g000034
rosa_roxburghii	Rroxscaffold_1G00008460
rosa_rugosa	Rorug01G0367900 Rorug01G0368000 Rorug01G0368100 Rorug01G0368200 Rorug01G0368300 Rorug05G0419200 Rorug05G0419300
rosa_samantha	Rh1AG377600 Rh1BG341000 Rh1CG354200 Rh1DG372000 Rh5AG476200 Rh5BG496400 Rh5CG520200 Rh5DG508800
rosa_wichuraiana	Rw1G033220 Rw5G044220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1065
AccB1I	GGYRCC	1 cut(s)	1522
AccB7I	CCANNNNNTGG	3 cut(s)	992, 1158, 1265
AccIII	TCCGGA	1 cut(s)	867
AciI	CCGC	2 cut(s)	1112, 1742
AclWI	GGATC	4 cut(s)	704, 767, 780, 1277
AcoI	YGGCCR	2 cut(s)	901, 1502
AcsI	RAATTY	5 cut(s)	563, 658, 677, 743, 873
AcuI	CTGAAG	1 cut(s)	798
AdeI	CACNNNGTG	1 cut(s)	518
AfaI	GTAC	7 cut(s)	234, 984, 1011, 1274, 1396, 1404, 1683
AfeI	AGCGCT	1 cut(s)	882
AfiI	CCNNNNNNNGG	8 cut(s)	105, 992, 1027, 1158, 1265, 1660, 1661, 1748
AflIII	ACRYGT	1 cut(s)	1405
AjnI	CCWGG	7 cut(s)	307, 354, 1151, 1258, 1450, 1499, 1518
AloI	GAACNNNNNNTCC	2 cut(s)	296, 328
AluBI	AGCT	9 cut(s)	50, 121, 266, 407, 731, 865, 977, 1086, 1552
AluI	AGCT	9 cut(s)	50, 121, 266, 407, 731, 865, 977, 1086, 1552
Alw21I	GWGCWC	2 cut(s)	442, 867
Alw26I	GTCTC	3 cut(s)	363, 629, 1486
Alw44I	GTGCAC	1 cut(s)	438
AlwI	GGATC	4 cut(s)	704, 767, 780, 1277
AlwNI	CAGNNNCTG	1 cut(s)	1265
Aor13HI	TCCGGA	1 cut(s)	867
Aor51HI	AGCGCT	1 cut(s)	882
AoxI	GGCC	4 cut(s)	799, 901, 1502, 1565
ApaLI	GTGCAC	1 cut(s)	438
ApoI	RAATTY	5 cut(s)	563, 658, 677, 743, 873
Asp700I	GAANNNNTTC	1 cut(s)	1591
AspLEI	GCGC	2 cut(s)	883, 1066
AspS9I	GGNCC	7 cut(s)	61, 352, 521, 599, 1565, 1636, 1651
AsuC2I	CCSGG	1 cut(s)	888
AsuHPI	GGTGA	5 cut(s)	275, 305, 661, 902, 1363
AvaII	GGWCC	6 cut(s)	61, 352, 521, 599, 1636, 1651
BaeGI	GKGCMC	2 cut(s)	442, 1527
BalI	TGGCCA	2 cut(s)	903, 1504
BamHI	GGATCC	1 cut(s)	772
BanI	GGYRCC	1 cut(s)	1522
BanII	GRGCYC	1 cut(s)	867
BarI	GAAGNNNNNNTAC	2 cut(s)	796, 828
BbsI	GAAGAC	1 cut(s)	264
Bbv12I	GWGCWC	2 cut(s)	442, 867
BccI	CCATC	2 cut(s)	206, 1513
BceAI	ACGGC	2 cut(s)	786, 1699
BciT130I	CCWGG	7 cut(s)	309, 356, 1153, 1260, 1452, 1501, 1520
BclI	TGATCA	2 cut(s)	399, 1177
BcnI	CCSGG	1 cut(s)	888
BcoDI	GTCTC	3 cut(s)	363, 629, 1486
BfaI	CTAG	6 cut(s)	267, 470, 732, 797, 878, 1632
BfmI	CTRYAG	4 cut(s)	277, 527, 556, 737
BfoI	RGCGCY	1 cut(s)	884
BglII	AGATCT	1 cut(s)	69
BlpI	GCTNAGC	1 cut(s)	51
BmcAI	AGTACT	2 cut(s)	234, 1396
Bme1390I	CCNGG	8 cut(s)	309, 356, 888, 1153, 1260, 1452, 1501, 1520
Bme18I	GGWCC	6 cut(s)	61, 352, 521, 599, 1636, 1651
BmgT120I	GGNCC	7 cut(s)	61, 352, 521, 599, 1565, 1636, 1651
BmiI	GGNNCC	7 cut(s)	62, 306, 774, 1524, 1566, 1637, 1653
BmrFI	CCNGG	8 cut(s)	309, 356, 888, 1153, 1260, 1452, 1501, 1520
BmrI	ACTGGG	1 cut(s)	1035
BmsI	GCATC	2 cut(s)	594, 1580
BmuI	ACTGGG	1 cut(s)	1035
BpiI	GAAGAC	1 cut(s)	264
Bpu1102I	GCTNAGC	1 cut(s)	51
BpuEI	CTTGAG	2 cut(s)	1107, 1451
BpuMI	CCSGG	1 cut(s)	888
Bsa29I	ATCGAT	1 cut(s)	126
BsaI	GGTCTC	1 cut(s)	363
BsaJI	CCNNGG	6 cut(s)	308, 445, 517, 672, 1499, 1518
BsaWI	WCCGGW	1 cut(s)	867
Bsc4I	CCNNNNNNNGG	8 cut(s)	105, 992, 1027, 1158, 1265, 1660, 1661, 1748
Bse1I	ACTGG	7 cut(s)	483, 602, 1030, 1270, 1381, 1402, 1720
Bse3DI	GCAATG	2 cut(s)	1253, 1574
BseAI	TCCGGA	1 cut(s)	867
BseBI	CCWGG	7 cut(s)	309, 356, 1153, 1260, 1452, 1501, 1520
BseCI	ATCGAT	1 cut(s)	126
BseDI	CCNNGG	6 cut(s)	308, 445, 517, 672, 1499, 1518
BseGI	GGATG	1 cut(s)	614
BseLI	CCNNNNNNNGG	8 cut(s)	105, 992, 1027, 1158, 1265, 1660, 1661, 1748
BseMI	GCAATG	2 cut(s)	1253, 1574
BseMII	CTCAG	4 cut(s)	42, 93, 156, 1544
BseNI	ACTGG	7 cut(s)	483, 602, 1030, 1270, 1381, 1402, 1720
BseRI	GAGGAG	2 cut(s)	124, 127
BseSI	GKGCMC	2 cut(s)	442, 1527
BshFI	GGCC	4 cut(s)	801, 903, 1504, 1567
BshNI	GGYRCC	1 cut(s)	1522
BshVI	ATCGAT	1 cut(s)	126
BsiHKAI	GWGCWC	2 cut(s)	442, 867
BsiSI	CCGG	2 cut(s)	868, 887
BslFI	GGGAC	4 cut(s)	969, 1043, 1637, 1649
BslI	CCNNNNNNNGG	8 cut(s)	105, 992, 1027, 1158, 1265, 1660, 1661, 1748
BsmAI	GTCTC	3 cut(s)	363, 629, 1486
BsmFI	GGGAC	4 cut(s)	969, 1043, 1637, 1649
BsmI	GAATGC	5 cut(s)	1153, 1198, 1591, 1593, 1671
BsnI	GGCC	4 cut(s)	801, 903, 1504, 1567
Bso31I	GGTCTC	1 cut(s)	363
Bsp1286I	GDGCHC	3 cut(s)	442, 867, 1527
Bsp13I	TCCGGA	1 cut(s)	867
Bsp1407I	TGTACA	3 cut(s)	1009, 1272, 1402
Bsp143I	GATC	8 cut(s)	69, 399, 709, 772, 1177, 1282, 1420, 1701
Bsp1720I	GCTNAGC	1 cut(s)	51
Bsp19I	CCATGG	1 cut(s)	445
BspACI	CCGC	2 cut(s)	1112, 1742
BspANI	GGCC	4 cut(s)	801, 903, 1504, 1567
BspCNI	CTCAG	4 cut(s)	43, 94, 157, 1543
BspDI	ATCGAT	1 cut(s)	126
BspEI	TCCGGA	1 cut(s)	867
BspHI	TCATGA	1 cut(s)	1075
BspLI	GGNNCC	7 cut(s)	62, 306, 774, 1524, 1566, 1637, 1653
BspMAI	CTGCAG	2 cut(s)	281, 741
BspPI	GGATC	4 cut(s)	704, 767, 780, 1277
BspQI	GCTCTTC	1 cut(s)	19
BspT107I	GGYRCC	1 cut(s)	1522
BspTNI	GGTCTC	1 cut(s)	363
BsrDI	GCAATG	2 cut(s)	1253, 1574
BsrGI	TGTACA	3 cut(s)	1009, 1272, 1402
BsrI	ACTGG	7 cut(s)	483, 602, 1030, 1270, 1381, 1402, 1720
BssECI	CCNNGG	6 cut(s)	308, 445, 517, 672, 1499, 1518
BssMI	GATC	8 cut(s)	69, 399, 709, 772, 1177, 1282, 1420, 1701
BssT1I	CCWWGG	2 cut(s)	445, 672
Bst2UI	CCWGG	7 cut(s)	309, 356, 1153, 1260, 1452, 1501, 1520
Bst4CI	ACNGT	3 cut(s)	232, 433, 666
Bst6I	CTCTTC	3 cut(s)	19, 361, 1515
BstAUI	TGTACA	3 cut(s)	1009, 1272, 1402
BstC8I	GCNNGC	3 cut(s)	829, 975, 1569
BstDEI	CTNAG	5 cut(s)	51, 102, 165, 262, 1530
BstDSI	CCRYGG	2 cut(s)	445, 517
BstF5I	GGATG	1 cut(s)	614
BstH2I	RGCGCY	1 cut(s)	884
BstHHI	GCGC	2 cut(s)	883, 1066
BstKTI	GATC	8 cut(s)	72, 402, 712, 775, 1180, 1285, 1423, 1704
BstMAI	GTCTC	3 cut(s)	363, 629, 1486
BstMBI	GATC	8 cut(s)	69, 399, 709, 772, 1177, 1282, 1420, 1701
BstNI	CCWGG	7 cut(s)	309, 356, 1153, 1260, 1452, 1501, 1520
BstNSI	RCATGY	2 cut(s)	1273, 1409
BstSCI	CCNGG	8 cut(s)	307, 354, 886, 1151, 1258, 1450, 1499, 1518
BstSFI	CTRYAG	4 cut(s)	277, 527, 556, 737
BstSLI	GKGCMC	2 cut(s)	442, 1527
BstV2I	GAAGAC	1 cut(s)	264
BstX2I	RGATCY	3 cut(s)	69, 709, 772
BstXI	CCANNNNNNTGG	1 cut(s)	362
BstYI	RGATCY	3 cut(s)	69, 709, 772
Bsu15I	ATCGAT	1 cut(s)	126
BsuRI	GGCC	4 cut(s)	801, 903, 1504, 1567
BsuTUI	ATCGAT	1 cut(s)	126
BtgI	CCRYGG	2 cut(s)	445, 517
BtsCI	GGATG	1 cut(s)	614
BtsI	GCAGTG	1 cut(s)	1073
BtsIMutI	CAGTG	4 cut(s)	476, 1023, 1073, 1170
Cac8I	GCNNGC	3 cut(s)	829, 975, 1569
CaiI	CAGNNNCTG	1 cut(s)	1265
CciI	TCATGA	1 cut(s)	1075
CfoI	GCGC	2 cut(s)	883, 1066
Cfr13I	GGNCC	7 cut(s)	61, 352, 521, 599, 1565, 1636, 1651
ClaI	ATCGAT	1 cut(s)	126
CsiI	ACCWGGT	1 cut(s)	354
Csp6I	GTAC	7 cut(s)	233, 983, 1010, 1273, 1395, 1403, 1682
CviAII	CATG	5 cut(s)	446, 1076, 1143, 1270, 1406
CviQI	GTAC	7 cut(s)	233, 983, 1010, 1273, 1395, 1403, 1682
DdeI	CTNAG	5 cut(s)	51, 102, 165, 262, 1530
DpnI	GATC	8 cut(s)	71, 401, 711, 774, 1179, 1284, 1422, 1703
DpnII	GATC	8 cut(s)	69, 399, 709, 772, 1177, 1282, 1420, 1701
DraI	TTTAAA	3 cut(s)	195, 382, 939
DraIII	CACNNNGTG	1 cut(s)	518
EaeI	YGGCCR	2 cut(s)	901, 1502
Eam1104I	CTCTTC	3 cut(s)	19, 361, 1515
EarI	CTCTTC	3 cut(s)	19, 361, 1515
EciI	GGCGGA	2 cut(s)	1101, 1731
Ecl136II	GAGCTC	1 cut(s)	865
Eco130I	CCWWGG	2 cut(s)	445, 672
Eco24I	GRGCYC	1 cut(s)	867
Eco31I	GGTCTC	1 cut(s)	363
Eco47I	GGWCC	6 cut(s)	61, 352, 521, 599, 1636, 1651
Eco47III	AGCGCT	1 cut(s)	882
Eco53kI	GAGCTC	1 cut(s)	865
Eco57I	CTGAAG	1 cut(s)	798
EcoICRI	GAGCTC	1 cut(s)	865
EcoO109I	RGGNCCY	3 cut(s)	1565, 1636, 1651
EcoRI	GAATTC	2 cut(s)	743, 873
EcoRII	CCWGG	7 cut(s)	307, 354, 1151, 1258, 1450, 1499, 1518
EcoT14I	CCWWGG	2 cut(s)	445, 672
EcoT22I	ATGCAT	3 cut(s)	1198, 1204, 1593
EcoT38I	GRGCYC	1 cut(s)	867
ErhI	CCWWGG	2 cut(s)	445, 672
FaeI	CATG	5 cut(s)	449, 1079, 1146, 1273, 1409
FaqI	GGGAC	4 cut(s)	969, 1043, 1637, 1649
FatI	CATG	5 cut(s)	445, 1075, 1142, 1269, 1405
FauNDI	CATATG	3 cut(s)	463, 1198, 1627
FbaI	TGATCA	2 cut(s)	399, 1177
FokI	GGATG	1 cut(s)	601
FriOI	GRGCYC	1 cut(s)	867
FspBI	CTAG	6 cut(s)	267, 470, 732, 797, 878, 1632
FspI	TGCGCA	1 cut(s)	1065
GlaI	GCGC	2 cut(s)	882, 1065
HaeII	RGCGCY	1 cut(s)	884
HaeIII	GGCC	4 cut(s)	801, 903, 1504, 1567
HapII	CCGG	2 cut(s)	868, 887
HhaI	GCGC	2 cut(s)	883, 1066
Hin1II	CATG	5 cut(s)	449, 1079, 1146, 1273, 1409
Hin6I	GCGC	2 cut(s)	881, 1064
HinP1I	GCGC	2 cut(s)	881, 1064
HincII	GTYRAC	3 cut(s)	45, 1378, 1676
HindII	GTYRAC	3 cut(s)	45, 1378, 1676
HindIII	AAGCTT	3 cut(s)	405, 1084, 1550
HinfI	GANTC	4 cut(s)	720, 1288, 1334, 1613
HpaI	GTTAAC	1 cut(s)	1378
HpaII	CCGG	2 cut(s)	868, 887
HphI	GGTGA	5 cut(s)	275, 305, 661, 902, 1363
Hpy166II	GTNNAC	9 cut(s)	45, 296, 352, 440, 521, 669, 1378, 1403, 1676
Hpy188III	TCNNGA	5 cut(s)	397, 816, 868, 1076, 1129
Hpy8I	GTNNAC	9 cut(s)	45, 296, 352, 440, 521, 669, 1378, 1403, 1676
HpyAV	CCTTC	5 cut(s)	296, 1128, 1320, 1642, 1755
HpyCH4III	ACNGT	3 cut(s)	232, 433, 666
HpyCH4IV	ACGT	3 cut(s)	200, 765, 1684
HpyF3I	CTNAG	5 cut(s)	51, 102, 165, 262, 1530
HpySE526I	ACGT	3 cut(s)	200, 765, 1684
Hsp92II	CATG	5 cut(s)	449, 1079, 1146, 1273, 1409
HspAI	GCGC	2 cut(s)	881, 1064
Kpn2I	TCCGGA	1 cut(s)	867
Ksp22I	TGATCA	2 cut(s)	399, 1177
KspAI	GTTAAC	1 cut(s)	1378
Kzo9I	GATC	8 cut(s)	69, 399, 709, 772, 1177, 1282, 1420, 1701
LguI	GCTCTTC	1 cut(s)	19
LmnI	GCTCC	2 cut(s)	118, 870
LweI	GCATC	2 cut(s)	594, 1580
MabI	ACCWGGT	1 cut(s)	354
MaeI	CTAG	6 cut(s)	267, 470, 732, 797, 878, 1632
MaeII	ACGT	3 cut(s)	200, 765, 1684
MaeIII	GTNAC	4 cut(s)	281, 808, 931, 1408
MalI	GATC	8 cut(s)	71, 401, 711, 774, 1179, 1284, 1422, 1703
MboI	GATC	8 cut(s)	69, 399, 709, 772, 1177, 1282, 1420, 1701
MflI	RGATCY	3 cut(s)	69, 709, 772
MhlI	GDGCHC	3 cut(s)	442, 867, 1527
MlsI	TGGCCA	2 cut(s)	903, 1504
MluCI	AATT	6 cut(s)	563, 577, 658, 677, 743, 873
MluNI	TGGCCA	2 cut(s)	903, 1504
MlyI	GAGTC	2 cut(s)	1297, 1343
MmeI	TCCRAC	2 cut(s)	343, 1704
Mox20I	TGGCCA	2 cut(s)	903, 1504
Mph1103I	ATGCAT	3 cut(s)	1198, 1204, 1593
MroI	TCCGGA	1 cut(s)	867
MroXI	GAANNNNTTC	1 cut(s)	1591
MscI	TGGCCA	2 cut(s)	903, 1504
MseI	TTAA	6 cut(s)	194, 381, 624, 834, 938, 1377
MslI	CAYNNNNRTG	1 cut(s)	1221
Msp20I	TGGCCA	2 cut(s)	903, 1504
MspA1I	CMGCKG	1 cut(s)	50
MspI	CCGG	2 cut(s)	868, 887
MspR9I	CCNGG	8 cut(s)	309, 356, 888, 1153, 1260, 1452, 1501, 1520
Mva1269I	GAATGC	5 cut(s)	1153, 1198, 1591, 1593, 1671
MvaI	CCWGG	7 cut(s)	309, 356, 1153, 1260, 1452, 1501, 1520
NciI	CCSGG	1 cut(s)	888
NcoI	CCATGG	1 cut(s)	445
NdeI	CATATG	3 cut(s)	463, 1198, 1627
NdeII	GATC	8 cut(s)	69, 399, 709, 772, 1177, 1282, 1420, 1701
NlaIII	CATG	5 cut(s)	449, 1079, 1146, 1273, 1409
NlaIV	GGNNCC	7 cut(s)	62, 306, 774, 1524, 1566, 1637, 1653
NmuCI	GTSAC	2 cut(s)	281, 931
NsbI	TGCGCA	1 cut(s)	1065
NsiI	ATGCAT	3 cut(s)	1198, 1204, 1593
NspI	RCATGY	2 cut(s)	1273, 1409
PagI	TCATGA	1 cut(s)	1075
PciI	ACATGT	1 cut(s)	1405
PciSI	GCTCTTC	1 cut(s)	19
PctI	GAATGC	5 cut(s)	1153, 1198, 1591, 1593, 1671
PdmI	GAANNNNTTC	1 cut(s)	1591
PfeI	GAWTC	2 cut(s)	720, 1613
PflFI	GACNNNGTC	2 cut(s)	356, 1159
PflMI	CCANNNNNTGG	3 cut(s)	992, 1158, 1265
PleI	GAGTC	2 cut(s)	1296, 1342
PpsI	GAGTC	2 cut(s)	1296, 1342
PpuMI	RGGWCCY	2 cut(s)	1636, 1651
PscI	ACATGT	1 cut(s)	1405
Psp124BI	GAGCTC	1 cut(s)	867
Psp5II	RGGWCCY	2 cut(s)	1636, 1651
Psp6I	CCWGG	7 cut(s)	307, 354, 1151, 1258, 1450, 1499, 1518
PspGI	CCWGG	7 cut(s)	307, 354, 1151, 1258, 1450, 1499, 1518
PspN4I	GGNNCC	7 cut(s)	62, 306, 774, 1524, 1566, 1637, 1653
PspPI	GGNCC	7 cut(s)	61, 352, 521, 599, 1565, 1636, 1651
PspPPI	RGGWCCY	2 cut(s)	1636, 1651
PstI	CTGCAG	2 cut(s)	281, 741
PstNI	CAGNNNCTG	1 cut(s)	1265
PsuI	RGATCY	3 cut(s)	69, 709, 772
PsyI	GACNNNGTC	2 cut(s)	356, 1159
PvuII	CAGCTG	1 cut(s)	50
RsaI	GTAC	7 cut(s)	234, 984, 1011, 1274, 1396, 1404, 1683
RsaNI	GTAC	7 cut(s)	233, 983, 1010, 1273, 1395, 1403, 1682
RseI	CAYNNNNRTG	1 cut(s)	1221
SacI	GAGCTC	1 cut(s)	867
SapI	GCTCTTC	1 cut(s)	19
SaqAI	TTAA	6 cut(s)	194, 381, 624, 834, 938, 1377
Sau3AI	GATC	8 cut(s)	69, 399, 709, 772, 1177, 1282, 1420, 1701
Sau96I	GGNCC	7 cut(s)	61, 352, 521, 599, 1565, 1636, 1651
ScaI	AGTACT	2 cut(s)	234, 1396
SchI	GAGTC	2 cut(s)	1297, 1343
ScrFI	CCNGG	8 cut(s)	309, 356, 888, 1153, 1260, 1452, 1501, 1520
SduI	GDGCHC	3 cut(s)	442, 867, 1527
SexAI	ACCWGGT	1 cut(s)	354
SfaNI	GCATC	2 cut(s)	594, 1580
SfcI	CTRYAG	4 cut(s)	277, 527, 556, 737
SinI	GGWCC	6 cut(s)	61, 352, 521, 599, 1636, 1651
SmiMI	CAYNNNNRTG	1 cut(s)	1221
SmlI	CTYRAG	2 cut(s)	1122, 1430
SmoI	CTYRAG	2 cut(s)	1122, 1430
Sse9I	AATT	6 cut(s)	563, 577, 658, 677, 743, 873
SsiI	CCGC	2 cut(s)	1112, 1742
SspI	AATATT	1 cut(s)	1056
SspMI	CTAG	6 cut(s)	267, 470, 732, 797, 878, 1632
SstI	GAGCTC	1 cut(s)	867
StyD4I	CCNGG	8 cut(s)	307, 354, 886, 1151, 1258, 1450, 1499, 1518
StyI	CCWWGG	2 cut(s)	445, 672
TaaI	ACNGT	3 cut(s)	232, 433, 666
TaiI	ACGT	3 cut(s)	203, 768, 1687
TaqI	TCGA	1 cut(s)	126
TasI	AATT	6 cut(s)	563, 577, 658, 677, 743, 873
TatI	WGTACW	5 cut(s)	232, 1009, 1272, 1394, 1402
TfiI	GAWTC	2 cut(s)	720, 1613
Tru1I	TTAA	6 cut(s)	194, 381, 624, 834, 938, 1377
Tru9I	TTAA	6 cut(s)	194, 381, 624, 834, 938, 1377
TscAI	CASTG	4 cut(s)	483, 1030, 1073, 1170
TseFI	GTSAC	2 cut(s)	281, 931
Tsp45I	GTSAC	2 cut(s)	281, 931
TspDTI	ATGAA	8 cut(s)	494, 498, 527, 1064, 1095, 1205, 1373, 1614
TspGWI	ACGGA	2 cut(s)	506, 607
TspRI	CASTG	4 cut(s)	483, 1030, 1073, 1170
Tth111I	GACNNNGTC	2 cut(s)	356, 1159
Van91I	CCANNNNNTGG	3 cut(s)	992, 1158, 1265
VneI	GTGCAC	1 cut(s)	438
VpaK11BI	GGWCC	6 cut(s)	61, 352, 521, 599, 1636, 1651
XapI	RAATTY	5 cut(s)	563, 658, 677, 743, 873
XceI	RCATGY	2 cut(s)	1273, 1409
XmnI	GAANNNNTTC	1 cut(s)	1591
XspI	CTAG	6 cut(s)	267, 470, 732, 797, 878, 1632
ZrmI	AGTACT	2 cut(s)	234, 1396
Zsp2I	ATGCAT	3 cut(s)	1198, 1204, 1593

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.