RchiOBHm_Chr1g0372011

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0001135
Physical Location & Seq
Forward (+)
60901003 .. 60907683
6681 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ59605

Sequence Viewer

Length: 1464 bp
ATGGAAGTTTGCTCTTCTTCTGAAATATCAATAGATAAATGGTTGACAGCTGAGCATAGGGTCCAAGAGATCTTATGGGCTGTTCAACCAAATGTAGCCTCTGAGTGGAGGAGGAGGGAGCTGATCGATTATGTTCAAAGGATAATCAAAGGTCATTTCGGAACTGAGGTGTTGTCCTTTGGTTCAGTTCCTTTAAAAACGTATCTCCCTGATGGAGATATTGATTTGACAGTACTCTGTCATCGGAAGCAGGAGGAAGACTTAGCTAGGGCTATCTGCAGTCACCTTCAAGGTGAACCAAAGGAACCTGGGTTCTTGGTTGAAGATGTTAGATATGTTTATGCAAAGGTTAAGATCATAAAATGCACTGTGAATAATATAGCAGTTGATATTTCATTTAATCAGGTGGCTGGGCTGTGTACACTGTGCTTTCTGGAGCAGGCGGACCAGGTCGTTGGAAGAGACCACCTTTTTAAAAAGAGTATAATCTTGATCAAAGCTTGGTGCTATTATGAGAGCCGAACCGTTGGTGCACCCCATGGCTTGATTTCAACATATGCACTAGAAACACTGGTCTTGTATATCATCAATCTCTTTCATTCATCACTCCGTGGACCTCTACAGGTTCTTCATAGATTTTTGGAATACTATAGCAAATTTGATTGGACAAATTATTGCATCAGTATAAATGGTCCAGTTTCCTTATCATCCGTTCTTAAAATCAGAGAGACACCAGAAAATGAGGGGGAGAATTTACTGTTCACCAAGGAATTTTTGAAGAAATGTAGAGTTGCATTTTCAGATCCAAGAAGAATCAGCGAAGCTAGACTGCAGGAATTCCCAATCAAGTGCTTCAACGTAGTGGATCCTCTAAAAGACAACAACAATCTAGGCCGTAGTGTTACTTCAGGAAACTTCGCTCGCATTAAATCTGCTTTTGCATTTGGAGAAAATGAGCTCCGGAGAATTCTAGCGCTACCAGGTGAAAACATTGGCCACAAACTTCAAAAAGATTTTTTTCCCGTCACTTTAAATAGGAATGGGAGGGGACAAAGACCAGATTTGCAAGCTCTTGTACCAGCATTTGGTAGTGGAAGATATGTACAATCTAACCTCACTGGGGACTTTGATTATCATTATGGGGGAATATTGTATGCACAGTGGCTTCATGATGAAAGCTTACATCTTTCTTCACACCCAAGTCCGCCACCTTCTCAAGTCCAGAATAACACCACATGGAATGCCAGGACTTGGTCAGTGAATGTCTTTGATCAAATGTCAATGAATGCATATGCATTACCTTTCTATCATCATTGTGCTTCCCAACAGACTGCTCATATAAGGACATTGCCAGGAACTGGCATGTACATACCTGATCCGAGTCCTCATTACAGACAGATGTATTCAAGGGCGAGGGGAAGGAACGAGTCTAAAAATCTTCGGGTGATGATGAAACCACCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NTP_transf_2 PAP_assoc

Protein Analysis

487

Amino Acids

55.64

Weight (kDa)

8.63

Isoelectric Point (pI)

46.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MTPAP-like_central PF22600 21 - 139 3.6e-12 Poly(A) RNA polymerase, mitochondrial-like, central palm domain
PAP-OAS1 PF26180 152 - 343 3.7e-85 PAP/OAS1 substrate-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 1085, 1251, 1358
AccIII TCCGGA 1 cut(s) 960
AciI CCGC 2 cut(s) 443, 1205
AclWI GGATC 4 cut(s) 797, 860, 873, 1370
AcoI YGGCCR 1 cut(s) 994
AcsI RAATTY 5 cut(s) 656, 751, 770, 836, 966
AcuI CTGAAG 1 cut(s) 891
AdeI CACNNNGTG 1 cut(s) 611
AfaI GTAC 5 cut(s) 234, 421, 1077, 1104, 1367
AfeI AGCGCT 1 cut(s) 975
AfiI CCNNNNNNNGG 5 cut(s) 105, 1085, 1120, 1251, 1358
AgsI TTSAA 9 cut(s) 86, 137, 290, 323, 552, 778, 856, 1007, 1407
AjnI CCWGG 5 cut(s) 307, 447, 979, 1244, 1351
AloI GAACNNNNNNTCC 2 cut(s) 296, 328
AluBI AGCT 8 cut(s) 50, 121, 266, 500, 824, 958, 1070, 1179
AluI AGCT 8 cut(s) 50, 121, 266, 500, 824, 958, 1070, 1179
Alw21I GWGCWC 2 cut(s) 535, 960
Alw26I GTCTC 2 cut(s) 456, 722
Alw44I GTGCAC 1 cut(s) 531
AlwI GGATC 4 cut(s) 797, 860, 873, 1370
AlwNI CAGNNNCTG 1 cut(s) 1358
Aor13HI TCCGGA 1 cut(s) 960
Aor51HI AGCGCT 1 cut(s) 975
AoxI GGCC 2 cut(s) 892, 994
ApaLI GTGCAC 1 cut(s) 531
ApoI RAATTY 5 cut(s) 656, 751, 770, 836, 966
AspLEI GCGC 1 cut(s) 976
AspS9I GGNCC 4 cut(s) 61, 445, 614, 692
AsuHPI GGTGA 5 cut(s) 275, 305, 754, 995, 1456
AvaII GGWCC 4 cut(s) 61, 445, 614, 692
BaeGI GKGCMC 1 cut(s) 535
BalI TGGCCA 1 cut(s) 996
BamHI GGATCC 1 cut(s) 865
BanII GRGCYC 1 cut(s) 960
BarI GAAGNNNNNNTAC 2 cut(s) 889, 921
BbsI GAAGAC 1 cut(s) 264
Bbv12I GWGCWC 2 cut(s) 535, 960
BccI CCATC 1 cut(s) 206
BceAI ACGGC 1 cut(s) 879
BciT130I CCWGG 5 cut(s) 309, 449, 981, 1246, 1353
BclI TGATCA 2 cut(s) 492, 1270
BcoDI GTCTC 2 cut(s) 456, 722
BfaI CTAG 5 cut(s) 267, 563, 825, 890, 971
BfmI CTRYAG 4 cut(s) 277, 620, 649, 830
BfoI RGCGCY 1 cut(s) 977
BglII AGATCT 1 cut(s) 69
BlpI GCTNAGC 1 cut(s) 51
BmcAI AGTACT 1 cut(s) 234
Bme1390I CCNGG 5 cut(s) 309, 449, 981, 1246, 1353
Bme18I GGWCC 4 cut(s) 61, 445, 614, 692
BmgT120I GGNCC 4 cut(s) 61, 445, 614, 692
BmiI GGNNCC 3 cut(s) 62, 306, 867
BmrFI CCNGG 5 cut(s) 309, 449, 981, 1246, 1353
BmrI ACTGGG 1 cut(s) 1128
BmsI GCATC 1 cut(s) 687
BmuI ACTGGG 1 cut(s) 1128
BpiI GAAGAC 1 cut(s) 264
BpmI CTGGAG 1 cut(s) 455
Bpu1102I GCTNAGC 1 cut(s) 51
BpuEI CTTGAG 1 cut(s) 1200
Bsa29I ATCGAT 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 456
BsaJI CCNNGG 4 cut(s) 308, 538, 610, 765
BsaWI WCCGGW 1 cut(s) 960
Bsc4I CCNNNNNNNGG 5 cut(s) 105, 1085, 1120, 1251, 1358
Bse1I ACTGG 4 cut(s) 576, 695, 1123, 1363
Bse3DI GCAATG 1 cut(s) 1346
BseAI TCCGGA 1 cut(s) 960
BseBI CCWGG 5 cut(s) 309, 449, 981, 1246, 1353
BseCI ATCGAT 1 cut(s) 126
BseDI CCNNGG 4 cut(s) 308, 538, 610, 765
BseGI GGATG 1 cut(s) 707
BseLI CCNNNNNNNGG 5 cut(s) 105, 1085, 1120, 1251, 1358
BseMI GCAATG 1 cut(s) 1346
BseMII CTCAG 3 cut(s) 42, 93, 156
BseNI ACTGG 4 cut(s) 576, 695, 1123, 1363
BseRI GAGGAG 2 cut(s) 124, 127
BseSI GKGCMC 1 cut(s) 535
BseYI CCCAGC 1 cut(s) 410
BshFI GGCC 2 cut(s) 894, 996
BshVI ATCGAT 1 cut(s) 126
BsiHKAI GWGCWC 2 cut(s) 535, 960
BsiSI CCGG 1 cut(s) 961
BslFI GGGAC 2 cut(s) 1062, 1136
BslI CCNNNNNNNGG 5 cut(s) 105, 1085, 1120, 1251, 1358
BsmAI GTCTC 2 cut(s) 456, 722
BsmFI GGGAC 2 cut(s) 1062, 1136
BsmI GAATGC 2 cut(s) 1246, 1291
BsnI GGCC 2 cut(s) 894, 996
Bso31I GGTCTC 1 cut(s) 456
Bsp1286I GDGCHC 2 cut(s) 535, 960
Bsp13I TCCGGA 1 cut(s) 960
Bsp1407I TGTACA 3 cut(s) 419, 1102, 1365
Bsp143I GATC 8 cut(s) 69, 123, 354, 492, 802, 865, 1270, 1375
Bsp1720I GCTNAGC 1 cut(s) 51
Bsp19I CCATGG 1 cut(s) 538
BspACI CCGC 2 cut(s) 443, 1205
BspANI GGCC 2 cut(s) 894, 996
BspCNI CTCAG 3 cut(s) 43, 94, 157
BspDI ATCGAT 1 cut(s) 126
BspEI TCCGGA 1 cut(s) 960
BspHI TCATGA 1 cut(s) 1168
BspLI GGNNCC 3 cut(s) 62, 306, 867
BspMAI CTGCAG 2 cut(s) 281, 834
BspPI GGATC 4 cut(s) 797, 860, 873, 1370
BspQI GCTCTTC 1 cut(s) 19
BspTNI GGTCTC 1 cut(s) 456
BsrDI GCAATG 1 cut(s) 1346
BsrGI TGTACA 3 cut(s) 419, 1102, 1365
BsrI ACTGG 4 cut(s) 576, 695, 1123, 1363
BssECI CCNNGG 4 cut(s) 308, 538, 610, 765
BssMI GATC 8 cut(s) 69, 123, 354, 492, 802, 865, 1270, 1375
BssT1I CCWWGG 2 cut(s) 538, 765
Bst2UI CCWGG 5 cut(s) 309, 449, 981, 1246, 1353
Bst4CI ACNGT 6 cut(s) 232, 370, 426, 526, 759, 1161
Bst6I CTCTTC 2 cut(s) 19, 454
BstAUI TGTACA 3 cut(s) 419, 1102, 1365
BstC8I GCNNGC 3 cut(s) 441, 922, 1068
BstDEI CTNAG 4 cut(s) 51, 102, 165, 262
BstDSI CCRYGG 2 cut(s) 538, 610
BstF5I GGATG 1 cut(s) 707
BstH2I RGCGCY 1 cut(s) 977
BstHHI GCGC 1 cut(s) 976
BstKTI GATC 8 cut(s) 72, 126, 357, 495, 805, 868, 1273, 1378
BstMAI GTCTC 2 cut(s) 456, 722
BstMBI GATC 8 cut(s) 69, 123, 354, 492, 802, 865, 1270, 1375
BstNI CCWGG 5 cut(s) 309, 449, 981, 1246, 1353
BstNSI RCATGY 1 cut(s) 1366
BstSCI CCNGG 5 cut(s) 307, 447, 979, 1244, 1351
BstSFI CTRYAG 4 cut(s) 277, 620, 649, 830
BstSLI GKGCMC 1 cut(s) 535
BstV2I GAAGAC 1 cut(s) 264
BstX2I RGATCY 3 cut(s) 69, 802, 865
BstXI CCANNNNNNTGG 1 cut(s) 455
BstYI RGATCY 3 cut(s) 69, 802, 865
Bsu15I ATCGAT 1 cut(s) 126
BsuRI GGCC 2 cut(s) 894, 996
BsuTUI ATCGAT 1 cut(s) 126
BtgI CCRYGG 2 cut(s) 538, 610
BtsCI GGATG 1 cut(s) 707
BtsIMutI CAGTG 6 cut(s) 366, 422, 569, 1116, 1166, 1263
Cac8I GCNNGC 3 cut(s) 441, 922, 1068
CaiI CAGNNNCTG 1 cut(s) 1358
CciI TCATGA 1 cut(s) 1168
CfoI GCGC 1 cut(s) 976
Cfr13I GGNCC 4 cut(s) 61, 445, 614, 692
ClaI ATCGAT 1 cut(s) 126
CsiI ACCWGGT 2 cut(s) 447, 979
Csp6I GTAC 5 cut(s) 233, 420, 1076, 1103, 1366
CviAII CATG 4 cut(s) 539, 1169, 1236, 1363
CviQI GTAC 5 cut(s) 233, 420, 1076, 1103, 1366
DdeI CTNAG 4 cut(s) 51, 102, 165, 262
DpnI GATC 8 cut(s) 71, 125, 356, 494, 804, 867, 1272, 1377
DpnII GATC 8 cut(s) 69, 123, 354, 492, 802, 865, 1270, 1375
DraI TTTAAA 3 cut(s) 195, 475, 1032
DraIII CACNNNGTG 1 cut(s) 611
EaeI YGGCCR 1 cut(s) 994
Eam1104I CTCTTC 2 cut(s) 19, 454
EarI CTCTTC 2 cut(s) 19, 454
EciI GGCGGA 2 cut(s) 458, 1194
Ecl136II GAGCTC 1 cut(s) 958
Eco130I CCWWGG 2 cut(s) 538, 765
Eco24I GRGCYC 1 cut(s) 960
Eco31I GGTCTC 1 cut(s) 456
Eco47I GGWCC 4 cut(s) 61, 445, 614, 692
Eco47III AGCGCT 1 cut(s) 975
Eco53kI GAGCTC 1 cut(s) 958
Eco57I CTGAAG 1 cut(s) 891
EcoICRI GAGCTC 1 cut(s) 958
EcoRI GAATTC 2 cut(s) 836, 966
EcoRII CCWGG 5 cut(s) 307, 447, 979, 1244, 1351
EcoT14I CCWWGG 2 cut(s) 538, 765
EcoT22I ATGCAT 2 cut(s) 1291, 1297
EcoT38I GRGCYC 1 cut(s) 960
ErhI CCWWGG 2 cut(s) 538, 765
FaeI CATG 4 cut(s) 542, 1172, 1239, 1366
FaqI GGGAC 2 cut(s) 1062, 1136
FatI CATG 4 cut(s) 538, 1168, 1235, 1362
FauNDI CATATG 2 cut(s) 556, 1291
FbaI TGATCA 2 cut(s) 492, 1270
FokI GGATG 1 cut(s) 694
FriOI GRGCYC 1 cut(s) 960
FspBI CTAG 5 cut(s) 267, 563, 825, 890, 971
GlaI GCGC 1 cut(s) 975
GsaI CCCAGC 1 cut(s) 414
GsuI CTGGAG 1 cut(s) 455
HaeII RGCGCY 1 cut(s) 977
HaeIII GGCC 2 cut(s) 894, 996
HapII CCGG 1 cut(s) 961
HhaI GCGC 1 cut(s) 976
Hin1II CATG 4 cut(s) 542, 1172, 1239, 1366
Hin6I GCGC 1 cut(s) 974
HinP1I GCGC 1 cut(s) 974
HincII GTYRAC 1 cut(s) 45
HindII GTYRAC 1 cut(s) 45
HindIII AAGCTT 2 cut(s) 498, 1177
HinfI GANTC 3 cut(s) 813, 1381, 1427
HpaII CCGG 1 cut(s) 961
HphI GGTGA 5 cut(s) 275, 305, 754, 995, 1456
Hpy166II GTNNAC 7 cut(s) 45, 296, 420, 422, 533, 614, 762
Hpy188I TCNGA 7 cut(s) 22, 103, 161, 246, 725, 802, 1380
Hpy188III TCNNGA 6 cut(s) 434, 490, 909, 961, 1169, 1222
Hpy8I GTNNAC 7 cut(s) 45, 296, 420, 422, 533, 614, 762
HpyAV CCTTC 3 cut(s) 296, 1221, 1413
HpyCH4III ACNGT 6 cut(s) 232, 370, 426, 526, 759, 1161
HpyCH4IV ACGT 2 cut(s) 200, 858
HpyF3I CTNAG 4 cut(s) 51, 102, 165, 262
HpySE526I ACGT 2 cut(s) 200, 858
Hsp92II CATG 4 cut(s) 542, 1172, 1239, 1366
HspAI GCGC 1 cut(s) 974
Kpn2I TCCGGA 1 cut(s) 960
Ksp22I TGATCA 2 cut(s) 492, 1270
Kzo9I GATC 8 cut(s) 69, 123, 354, 492, 802, 865, 1270, 1375
LguI GCTCTTC 1 cut(s) 19
LmnI GCTCC 3 cut(s) 118, 436, 963
LweI GCATC 1 cut(s) 687
MabI ACCWGGT 2 cut(s) 447, 979
MaeI CTAG 5 cut(s) 267, 563, 825, 890, 971
MaeII ACGT 2 cut(s) 200, 858
MaeIII GTNAC 3 cut(s) 281, 901, 1024
MalI GATC 8 cut(s) 71, 125, 356, 494, 804, 867, 1272, 1377
MboI GATC 8 cut(s) 69, 123, 354, 492, 802, 865, 1270, 1375
MflI RGATCY 3 cut(s) 69, 802, 865
MhlI GDGCHC 2 cut(s) 535, 960
MlsI TGGCCA 1 cut(s) 996
MluCI AATT 6 cut(s) 656, 670, 751, 770, 836, 966
MluNI TGGCCA 1 cut(s) 996
MlyI GAGTC 2 cut(s) 1390, 1436
MmeI TCCRAC 1 cut(s) 436
Mox20I TGGCCA 1 cut(s) 996
Mph1103I ATGCAT 2 cut(s) 1291, 1297
MroI TCCGGA 1 cut(s) 960
MscI TGGCCA 1 cut(s) 996
MseI TTAA 7 cut(s) 194, 351, 399, 474, 717, 927, 1031
MslI CAYNNNNRTG 1 cut(s) 1314
Msp20I TGGCCA 1 cut(s) 996
MspA1I CMGCKG 1 cut(s) 50
MspI CCGG 1 cut(s) 961
MspR9I CCNGG 5 cut(s) 309, 449, 981, 1246, 1353
Mva1269I GAATGC 2 cut(s) 1246, 1291
MvaI CCWGG 5 cut(s) 309, 449, 981, 1246, 1353
NcoI CCATGG 1 cut(s) 538
NdeI CATATG 2 cut(s) 556, 1291
NdeII GATC 8 cut(s) 69, 123, 354, 492, 802, 865, 1270, 1375
NlaIII CATG 4 cut(s) 542, 1172, 1239, 1366
NlaIV GGNNCC 3 cut(s) 62, 306, 867
NmuCI GTSAC 2 cut(s) 281, 1024
NsiI ATGCAT 2 cut(s) 1291, 1297
NspI RCATGY 1 cut(s) 1366
PagI TCATGA 1 cut(s) 1168
PciSI GCTCTTC 1 cut(s) 19
PctI GAATGC 2 cut(s) 1246, 1291
PfeI GAWTC 1 cut(s) 813
PflFI GACNNNGTC 2 cut(s) 449, 1252
PflMI CCANNNNNTGG 3 cut(s) 1085, 1251, 1358
PleI GAGTC 2 cut(s) 1389, 1435
PpsI GAGTC 2 cut(s) 1389, 1435
Psp124BI GAGCTC 1 cut(s) 960
Psp6I CCWGG 5 cut(s) 307, 447, 979, 1244, 1351
PspFI CCCAGC 1 cut(s) 410
PspGI CCWGG 5 cut(s) 307, 447, 979, 1244, 1351
PspN4I GGNNCC 3 cut(s) 62, 306, 867
PspPI GGNCC 4 cut(s) 61, 445, 614, 692
PstI CTGCAG 2 cut(s) 281, 834
PstNI CAGNNNCTG 1 cut(s) 1358
PsuI RGATCY 3 cut(s) 69, 802, 865
PsyI GACNNNGTC 2 cut(s) 449, 1252
PvuII CAGCTG 1 cut(s) 50
RsaI GTAC 5 cut(s) 234, 421, 1077, 1104, 1367
RsaNI GTAC 5 cut(s) 233, 420, 1076, 1103, 1366
RseI CAYNNNNRTG 1 cut(s) 1314
SacI GAGCTC 1 cut(s) 960
SapI GCTCTTC 1 cut(s) 19
SaqAI TTAA 7 cut(s) 194, 351, 399, 474, 717, 927, 1031
Sau3AI GATC 8 cut(s) 69, 123, 354, 492, 802, 865, 1270, 1375
Sau96I GGNCC 4 cut(s) 61, 445, 614, 692
ScaI AGTACT 1 cut(s) 234
SchI GAGTC 2 cut(s) 1390, 1436
ScrFI CCNGG 5 cut(s) 309, 449, 981, 1246, 1353
SduI GDGCHC 2 cut(s) 535, 960
SexAI ACCWGGT 2 cut(s) 447, 979
SfaNI GCATC 1 cut(s) 687
SfcI CTRYAG 4 cut(s) 277, 620, 649, 830
SinI GGWCC 4 cut(s) 61, 445, 614, 692
SmiMI CAYNNNNRTG 1 cut(s) 1314
SmlI CTYRAG 1 cut(s) 1215
SmoI CTYRAG 1 cut(s) 1215
Sse9I AATT 6 cut(s) 656, 670, 751, 770, 836, 966
SsiI CCGC 2 cut(s) 443, 1205
SspI AATATT 1 cut(s) 1149
SspMI CTAG 5 cut(s) 267, 563, 825, 890, 971
SstI GAGCTC 1 cut(s) 960
StyD4I CCNGG 5 cut(s) 307, 447, 979, 1244, 1351
StyI CCWWGG 2 cut(s) 538, 765
TaaI ACNGT 6 cut(s) 232, 370, 426, 526, 759, 1161
TaiI ACGT 2 cut(s) 203, 861
TaqI TCGA 1 cut(s) 126
TasI AATT 6 cut(s) 656, 670, 751, 770, 836, 966
TatI WGTACW 4 cut(s) 232, 419, 1102, 1365
TfiI GAWTC 1 cut(s) 813
Tru1I TTAA 7 cut(s) 194, 351, 399, 474, 717, 927, 1031
Tru9I TTAA 7 cut(s) 194, 351, 399, 474, 717, 927, 1031
TscAI CASTG 6 cut(s) 373, 429, 576, 1123, 1166, 1263
TseFI GTSAC 2 cut(s) 281, 1024
Tsp45I GTSAC 2 cut(s) 281, 1024
TspDTI ATGAA 7 cut(s) 384, 587, 591, 620, 1157, 1188, 1298
TspGWI ACGGA 2 cut(s) 599, 700
TspRI CASTG 6 cut(s) 373, 429, 576, 1123, 1166, 1263
Tth111I GACNNNGTC 2 cut(s) 449, 1252
Van91I CCANNNNNTGG 3 cut(s) 1085, 1251, 1358
VneI GTGCAC 1 cut(s) 531
VpaK11BI GGWCC 4 cut(s) 61, 445, 614, 692
XapI RAATTY 5 cut(s) 656, 751, 770, 836, 966
XceI RCATGY 1 cut(s) 1366
XspI CTAG 5 cut(s) 267, 563, 825, 890, 971
ZrmI AGTACT 1 cut(s) 234
Zsp2I ATGCAT 2 cut(s) 1291, 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.