AT4G24750

Rhodanese Homology Domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0002465
Physical Location & Seq
Reverse (-)
12757958 .. 12760934
2977 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G24750.1

Sequence Viewer

Length: 879 bp
ATGGAGTCTCTATCTCTTCCCGTTCTAAATCCTCTGTTAGCTTCTGGTTCTAATCTTTTTAGAAACCAGCACTCGAGGATGACCTCTTCAATGGTATCTTCACTTAAATCCCCCATTGGAGGCACATCGCTCTCCACTGTTCGTCGCTTTGGAGTAGGTGTTGTTAGGATGCAAGCTGTTGATGAAGATATTGATTTGAAGCAAATGAGAGACATAGCTGCTGCTAAAAAGCGTTGGGATGGTTTGTTAAGGGAAGGAAAAGTGAAGCTTCTTACACCAAGAGAAGCAGGCTATGCTATTTCTCTTTCAAACAAACCTCTCCTTGATGTTCGTCCCTCTAGCGAGCGCAACAAGGCATGGATTAAAGGCTCCACTTGGGTTCCTATCTTCGACAATGATGACAACCTTGACGCTGGAACTCTTTCCAAAAAAGTCACTAGTTTTGCTATGGGGGGATGGTGGAGTGGAGCACCTACACTATCTTTTAATAGGCTCTTCTTGTCAAAGGTTGAGGAGAAATTCCCAAAAGACTCAGAGCTGATTGTTGCTTGCCAAAAAGGATTGAGATCCTTAGCTGCTTGCGAGCTACTATATAATGCAGGGTATGAGAATCTGTTCTGGGTACAAGGTGGTTTAGAATCTGCTCAAGACGAGGATCTAGTGACAGAAGGTGTCCAACCCTTGAAGCTTGCTGGTATTGGAGGTTTTTCAGAGTTCCTCGGTTGGACAGACCAGCAAAGAGCTCAAGCTGCGAAAGAAGGTTGGGGGTACCGTTTGGTATATACTGCTCGTCTGTTTGGAGTTGTTCTGGCTGCTGATGCTTTGTTCGTTGGGGCTCAGCAACTAGGCCATTATATTCAGGAGTTAAGAGGCCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

292

Amino Acids

32.11

Weight (kDa)

8.77

Isoelectric Point (pI)

36.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 106 - 215 1.1e-08 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 768
AccB1I GGYRCC 1 cut(s) 768
AclWI GGATC 2 cut(s) 561, 663
AcsI RAATTY 1 cut(s) 518
AfaI GTAC 2 cut(s) 624, 770
AfiI CCNNNNNNNGG 1 cut(s) 119
AgsI TTSAA 4 cut(s) 90, 199, 309, 685
AhlI ACTAGT 1 cut(s) 437
Alw21I GWGCWC 2 cut(s) 472, 745
Alw26I GTCTC 2 cut(s) 12, 204
AlwI GGATC 2 cut(s) 561, 663
Ama87I CYCGRG 1 cut(s) 73
AoxI GGCC 2 cut(s) 847, 871
ApeKI GCWGC 5 cut(s) 218, 221, 575, 749, 812
ApoI RAATTY 1 cut(s) 518
Asp700I GAANNNNTTC 2 cut(s) 421, 614
Asp718I GGTACC 1 cut(s) 768
AspLEI GCGC 1 cut(s) 348
AvaI CYCGRG 1 cut(s) 73
BanI GGYRCC 1 cut(s) 768
BanII GRGCYC 2 cut(s) 745, 838
Bbv12I GWGCWC 2 cut(s) 472, 745
BbvI GCAGC 5 cut(s) 205, 208, 562, 736, 799
BccI CCATC 2 cut(s) 233, 450
BcoDI GTCTC 2 cut(s) 12, 204
BcuI ACTAGT 1 cut(s) 437
BfaI CTAG 4 cut(s) 339, 438, 659, 845
BisI GCNGC 5 cut(s) 219, 222, 576, 750, 813
BlpI GCTNAGC 1 cut(s) 837
BlsI GCNGC 5 cut(s) 220, 223, 577, 751, 814
BmeT110I CYCGRG 1 cut(s) 73
BmiI GGNNCC 3 cut(s) 370, 381, 770
BmsI GCATC 2 cut(s) 159, 808
Bpu10I CCTNAGC 1 cut(s) 571
Bpu1102I GCTNAGC 1 cut(s) 837
BpuEI CTTGAG 2 cut(s) 630, 729
BsaBI GATNNNNATC 1 cut(s) 565
BsaJI CCNNGG 1 cut(s) 718
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 119
Bse8I GATNNNNATC 1 cut(s) 565
BseDI CCNNGG 1 cut(s) 718
BseGI GGATG 4 cut(s) 84, 174, 244, 461
BseJI GATNNNNATC 1 cut(s) 565
BseLI CCNNNNNNNGG 1 cut(s) 119
BseMII CTCAG 2 cut(s) 546, 851
BseRI GAGGAG 1 cut(s) 527
BseXI GCAGC 5 cut(s) 205, 208, 562, 736, 799
BshFI GGCC 2 cut(s) 849, 873
BshNI GGYRCC 1 cut(s) 768
BsiHKAI GWGCWC 2 cut(s) 472, 745
BsiHKCI CYCGRG 1 cut(s) 73
BslFI GGGAC 1 cut(s) 318
BslI CCNNNNNNNGG 1 cut(s) 119
BsmAI GTCTC 2 cut(s) 12, 204
BsmFI GGGAC 1 cut(s) 318
BsnI GGCC 2 cut(s) 849, 873
BsoBI CYCGRG 1 cut(s) 73
Bsp1286I GDGCHC 3 cut(s) 472, 745, 838
Bsp143I GATC 2 cut(s) 566, 655
Bsp1720I GCTNAGC 1 cut(s) 837
BspANI GGCC 2 cut(s) 849, 873
BspCNI CTCAG 2 cut(s) 545, 850
BspLI GGNNCC 3 cut(s) 370, 381, 770
BspPI GGATC 2 cut(s) 561, 663
BspQI GCTCTTC 1 cut(s) 500
BspT107I GGYRCC 1 cut(s) 768
BssECI CCNNGG 1 cut(s) 718
BssMI GATC 2 cut(s) 566, 655
Bst4CI ACNGT 2 cut(s) 139, 773
Bst6I CTCTTC 3 cut(s) 21, 91, 500
BstAPI GCANNNNNTGC 1 cut(s) 293
BstC8I GCNNGC 7 cut(s) 174, 289, 344, 550, 580, 584, 690
BstDEI CTNAG 3 cut(s) 532, 571, 837
BstF5I GGATG 4 cut(s) 84, 174, 244, 461
BstHHI GCGC 1 cut(s) 348
BstKTI GATC 2 cut(s) 569, 658
BstMAI GTCTC 2 cut(s) 12, 204
BstMBI GATC 2 cut(s) 566, 655
BstMWI GCNNNNNNNGC 3 cut(s) 293, 749, 818
BstV1I GCAGC 5 cut(s) 205, 208, 562, 736, 799
BstX2I RGATCY 2 cut(s) 566, 655
BstYI RGATCY 2 cut(s) 566, 655
BsuRI GGCC 2 cut(s) 849, 873
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 4 cut(s) 84, 174, 244, 461
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 7 cut(s) 174, 289, 344, 550, 580, 584, 690
CfoI GCGC 1 cut(s) 348
CseI GACGC 1 cut(s) 419
Csp6I GTAC 2 cut(s) 623, 769
CviAII CATG 1 cut(s) 357
CviQI GTAC 2 cut(s) 623, 769
DdeI CTNAG 3 cut(s) 532, 571, 837
DpnI GATC 2 cut(s) 568, 657
DpnII GATC 2 cut(s) 566, 655
Eam1104I CTCTTC 3 cut(s) 21, 91, 500
EarI CTCTTC 3 cut(s) 21, 91, 500
Ecl136II GAGCTC 1 cut(s) 743
Eco24I GRGCYC 2 cut(s) 745, 838
Eco53kI GAGCTC 1 cut(s) 743
Eco88I CYCGRG 1 cut(s) 73
EcoICRI GAGCTC 1 cut(s) 743
EcoT38I GRGCYC 2 cut(s) 745, 838
FaeI CATG 1 cut(s) 360
FalI AAGNNNNNCTT 2 cut(s) 252, 284
FaqI GGGAC 1 cut(s) 318
FatI CATG 1 cut(s) 356
Fnu4HI GCNGC 5 cut(s) 219, 222, 576, 750, 813
FokI GGATG 4 cut(s) 91, 181, 251, 468
FriOI GRGCYC 2 cut(s) 745, 838
Fsp4HI GCNGC 5 cut(s) 219, 222, 576, 750, 813
FspBI CTAG 4 cut(s) 339, 438, 659, 845
GlaI GCGC 1 cut(s) 347
GluI GCNGC 5 cut(s) 219, 222, 576, 750, 813
HaeIII GGCC 2 cut(s) 849, 873
HgaI GACGC 1 cut(s) 419
HhaI GCGC 1 cut(s) 348
Hin1II CATG 1 cut(s) 360
Hin6I GCGC 1 cut(s) 346
HinP1I GCGC 1 cut(s) 346
HindIII AAGCTT 2 cut(s) 266, 686
HinfI GANTC 4 cut(s) 5, 530, 610, 638
Hpy188I TCNGA 2 cut(s) 535, 712
Hpy188III TCNNGA 2 cut(s) 647, 860
Hpy99I CGWCG 1 cut(s) 147
HpyAV CCTTC 3 cut(s) 248, 662, 752
HpyCH4III ACNGT 2 cut(s) 139, 773
HpyCH4V TGCA 2 cut(s) 172, 599
HpyF10VI GCNNNNNNNGC 3 cut(s) 293, 749, 818
HpyF3I CTNAG 3 cut(s) 532, 571, 837
Hsp92II CATG 1 cut(s) 360
HspAI GCGC 1 cut(s) 346
KpnI GGTACC 1 cut(s) 772
Kzo9I GATC 2 cut(s) 566, 655
LguI GCTCTTC 1 cut(s) 500
LmnI GCTCC 2 cut(s) 374, 467
Lsp1109I GCAGC 5 cut(s) 205, 208, 562, 736, 799
LweI GCATC 2 cut(s) 159, 808
MaeI CTAG 4 cut(s) 339, 438, 659, 845
MaeIII GTNAC 2 cut(s) 433, 661
MalI GATC 2 cut(s) 568, 657
MboI GATC 2 cut(s) 566, 655
MboII GAAGA 6 cut(s) 8, 78, 90, 197, 379, 487
MflI RGATCY 2 cut(s) 566, 655
MhlI GDGCHC 3 cut(s) 472, 745, 838
MluCI AATT 1 cut(s) 518
MlyI GAGTC 2 cut(s) 14, 524
MmeI TCCRAC 2 cut(s) 700, 704
MroXI GAANNNNTTC 2 cut(s) 421, 614
MseI TTAA 5 cut(s) 105, 248, 363, 486, 866
MwoI GCNNNNNNNGC 3 cut(s) 293, 749, 818
NdeII GATC 2 cut(s) 566, 655
NlaIII CATG 1 cut(s) 360
NlaIV GGNNCC 3 cut(s) 370, 381, 770
NmuCI GTSAC 2 cut(s) 433, 661
PaeR7I CTCGAG 1 cut(s) 73
PciSI GCTCTTC 1 cut(s) 500
PdmI GAANNNNTTC 2 cut(s) 421, 614
PfeI GAWTC 2 cut(s) 610, 638
PkrI GCNGC 5 cut(s) 220, 223, 577, 751, 814
PleI GAGTC 2 cut(s) 13, 524
PpsI GAGTC 2 cut(s) 13, 524
Psp124BI GAGCTC 1 cut(s) 745
PspN4I GGNNCC 3 cut(s) 370, 381, 770
PspXI VCTCGAGB 1 cut(s) 73
PsuI RGATCY 2 cut(s) 566, 655
RsaI GTAC 2 cut(s) 624, 770
RsaNI GTAC 2 cut(s) 623, 769
SacI GAGCTC 1 cut(s) 745
SapI GCTCTTC 1 cut(s) 500
SaqAI TTAA 5 cut(s) 105, 248, 363, 486, 866
SatI GCNGC 5 cut(s) 219, 222, 576, 750, 813
Sau3AI GATC 2 cut(s) 566, 655
SchI GAGTC 2 cut(s) 14, 524
SduI GDGCHC 3 cut(s) 472, 745, 838
SfaNI GCATC 2 cut(s) 159, 808
Sfr274I CTCGAG 1 cut(s) 73
SlaI CTCGAG 1 cut(s) 73
SmlI CTYRAG 3 cut(s) 73, 645, 744
SmoI CTYRAG 3 cut(s) 73, 645, 744
SpeI ACTAGT 1 cut(s) 437
Sse9I AATT 1 cut(s) 518
SspMI CTAG 4 cut(s) 339, 438, 659, 845
SstI GAGCTC 1 cut(s) 745
TaaI ACNGT 2 cut(s) 139, 773
TaqI TCGA 2 cut(s) 74, 390
TasI AATT 1 cut(s) 518
TfiI GAWTC 2 cut(s) 610, 638
Tru1I TTAA 5 cut(s) 105, 248, 363, 486, 866
Tru9I TTAA 5 cut(s) 105, 248, 363, 486, 866
TscAI CASTG 1 cut(s) 142
TseFI GTSAC 2 cut(s) 433, 661
TseI GCWGC 5 cut(s) 218, 221, 575, 749, 812
Tsp45I GTSAC 2 cut(s) 433, 661
TspDTI ATGAA 1 cut(s) 198
TspRI CASTG 1 cut(s) 142
XapI RAATTY 1 cut(s) 518
XhoI CTCGAG 1 cut(s) 73
XmnI GAANNNNTTC 2 cut(s) 421, 614
XspI CTAG 4 cut(s) 339, 438, 659, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.