Rw7G010550

Rhodanese-like domain-containing protein 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0002465
Physical Location & Seq
Forward (+)
9816153 .. 9820128
3976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G010550.1

Sequence Viewer

Length: 882 bp
ATGGAAGCTCTGGGATTTCCTTCTCTGAATACTCACACCATTTCGTGCTCTAGTTTTCGGCAACGAACACCCACCAAATTACCATATGAAACAAACCATTTCTCAAGCTCTTCTTCAAAACCAAAAGGACCCTCTTACAGAACCCGCCATTTTCGTAGGAGTGCTACACGAATGCAAGCTGACAATGAAGATTTCGAGTTGAAGCAAATGAGAGATATGGCTGCTGCCAAGAAAAGATGGGATGCTCTGATTAGGGACGGAAAGGTTAAACCTTTAACACCAAGGGAAGCTGGGTATGCAATTCAGCTATCTAACAAAACCCTTCTTGATGTTCGTCCCTCTACAGAACACAAAAAGGCATGGGTCAAAGGCTCAACCTGGATTCCAATATTTGATGTTGGTGACGAATTCGATGTTGGAACTCTTTCCAGAAAGATTATGGGATTCACTATGGGGGGTTGGTGGAGTGGTGTGCCTACATTGGCGTATAACAACCAGTTCTTGTCTAAGGTTGATGAGAAATTTCCAAAGGATACTGATCTTATCGTTGCATGCCAGAGGGGATTGAGATCTATAGCTGCTTGTGAGGTGCTGTACAATGCTGGCTACAGAAACCTTTTCTGGGTTCAGGGGGGTTTAGAAGCTGCTGAAGAAGAGGATCTTGTCAGAGAAGGCCCTCAGCCATTTAAGTTTGCAGGAATCGGTGGGGTTTCGGAGTTCCTCGGTTGGACTGATCAACAAAGAGCTGCAGCTGCTAAAGAAGGGTGGGGTTACCGATTTATGTTCTCTGCCCGTCTGGTTGGAGTCTTTCTGATTGCTGATGCCTTATTTCTTGGTTCTCAGCAACTAGTTCATTATCTTCAGGATATAAGGTCACACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

293

Amino Acids

33.09

Weight (kDa)

9.26

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 105 - 216 5.7e-09 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 145
AclWI GGATC 1 cut(s) 666
AcsI RAATTY 2 cut(s) 407, 521
AcuI CTGAAG 2 cut(s) 669, 845
AfaI GTAC 1 cut(s) 596
AfiI CCNNNNNNNGG 1 cut(s) 622
AgsI TTSAA 2 cut(s) 117, 202
AhlI ACTAGT 1 cut(s) 847
AjnI CCWGG 1 cut(s) 377
AjuI GAANNNNNNNTTGG 2 cut(s) 399, 431
AluBI AGCT 9 cut(s) 8, 108, 179, 290, 307, 578, 644, 746, 752
AluI AGCT 9 cut(s) 8, 108, 179, 290, 307, 578, 644, 746, 752
Alw21I GWGCWC 1 cut(s) 50
AlwI GGATC 1 cut(s) 666
AoxI GGCC 1 cut(s) 673
ApeKI GCWGC 7 cut(s) 221, 224, 578, 644, 746, 749, 752
ApoI RAATTY 2 cut(s) 407, 521
Asp700I GAANNNNTTC 1 cut(s) 424
AspS9I GGNCC 2 cut(s) 128, 674
AsuHPI GGTGA 1 cut(s) 413
AvaII GGWCC 1 cut(s) 128
Bbv12I GWGCWC 1 cut(s) 50
BbvCI CCTCAGC 1 cut(s) 678
BbvI GCAGC 7 cut(s) 208, 211, 565, 631, 733, 739, 761
BccI CCATC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 379
BciVI GTATCC 1 cut(s) 526
BclI TGATCA 1 cut(s) 733
BcuI ACTAGT 1 cut(s) 847
BfaI CTAG 2 cut(s) 51, 848
BfmI CTRYAG 4 cut(s) 342, 573, 607, 747
BfuI GTATCC 1 cut(s) 526
BglII AGATCT 1 cut(s) 569
BisI GCNGC 7 cut(s) 222, 225, 579, 645, 747, 750, 753
BlsI GCNGC 7 cut(s) 223, 226, 580, 646, 748, 751, 754
Bme1390I CCNGG 1 cut(s) 379
Bme18I GGWCC 1 cut(s) 128
BmgT120I GGNCC 2 cut(s) 128, 674
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 379
BmsI GCATC 2 cut(s) 232, 811
Bpu10I CCTNAGC 1 cut(s) 678
BpuEI CTTGAG 1 cut(s) 88
BsaBI GATNNNNATC 2 cut(s) 537, 568
BsaJI CCNNGG 2 cut(s) 281, 721
Bsc4I CCNNNNNNNGG 1 cut(s) 622
Bse1I ACTGG 1 cut(s) 496
Bse8I GATNNNNATC 2 cut(s) 537, 568
BseBI CCWGG 1 cut(s) 379
BseDI CCNNGG 2 cut(s) 281, 721
BseGI GGATG 1 cut(s) 247
BseJI GATNNNNATC 2 cut(s) 537, 568
BseLI CCNNNNNNNGG 1 cut(s) 622
BseMII CTCAG 2 cut(s) 692, 854
BseNI ACTGG 1 cut(s) 496
BseXI GCAGC 7 cut(s) 208, 211, 565, 631, 733, 739, 761
BseYI CCCAGC 1 cut(s) 290
BshFI GGCC 1 cut(s) 675
BsiHKAI GWGCWC 1 cut(s) 50
BslFI GGGAC 2 cut(s) 269, 321
BslI CCNNNNNNNGG 1 cut(s) 622
BsmFI GGGAC 2 cut(s) 269, 321
BsmI GAATGC 1 cut(s) 177
BsnI GGCC 1 cut(s) 675
Bsp1286I GDGCHC 1 cut(s) 50
Bsp1407I TGTACA 1 cut(s) 594
Bsp143I GATC 4 cut(s) 538, 569, 658, 733
BspACI CCGC 1 cut(s) 145
BspANI GGCC 1 cut(s) 675
BspCNI CTCAG 2 cut(s) 691, 853
BspLI GGNNCC 1 cut(s) 130
BspMAI CTGCAG 1 cut(s) 751
BspPI GGATC 1 cut(s) 666
BspQI GCTCTTC 1 cut(s) 115
BsrGI TGTACA 1 cut(s) 594
BsrI ACTGG 1 cut(s) 496
BssECI CCNNGG 2 cut(s) 281, 721
BssMI GATC 4 cut(s) 538, 569, 658, 733
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 379
Bst6I CTCTTC 2 cut(s) 115, 648
BstAUI TGTACA 1 cut(s) 594
BstC8I GCNNGC 3 cut(s) 177, 553, 604
BstDEI CTNAG 3 cut(s) 507, 678, 840
BstEII GGTNACC 1 cut(s) 770
BstF5I GGATG 1 cut(s) 247
BstKTI GATC 4 cut(s) 541, 572, 661, 736
BstMBI GATC 4 cut(s) 538, 569, 658, 733
BstMWI GCNNNNNNNGC 2 cut(s) 296, 752
BstNI CCWGG 1 cut(s) 379
BstNSI RCATGY 1 cut(s) 555
BstPI GGTNACC 1 cut(s) 770
BstSCI CCNGG 1 cut(s) 377
BstSFI CTRYAG 4 cut(s) 342, 573, 607, 747
BstV1I GCAGC 7 cut(s) 208, 211, 565, 631, 733, 739, 761
BstX2I RGATCY 2 cut(s) 569, 658
BstYI RGATCY 2 cut(s) 569, 658
BsuI GTATCC 1 cut(s) 526
BsuRI GGCC 1 cut(s) 675
BtsCI GGATG 1 cut(s) 247
Cac8I GCNNGC 3 cut(s) 177, 553, 604
Cfr13I GGNCC 2 cut(s) 128, 674
Csp6I GTAC 1 cut(s) 595
CviAII CATG 2 cut(s) 360, 552
CviQI GTAC 1 cut(s) 595
DdeI CTNAG 3 cut(s) 507, 678, 840
DpnI GATC 4 cut(s) 540, 571, 660, 735
DpnII GATC 4 cut(s) 538, 569, 658, 733
Eam1104I CTCTTC 2 cut(s) 115, 648
EarI CTCTTC 2 cut(s) 115, 648
Eco130I CCWWGG 1 cut(s) 281
Eco47I GGWCC 1 cut(s) 128
Eco57I CTGAAG 2 cut(s) 669, 845
Eco91I GGTNACC 1 cut(s) 770
EcoO109I RGGNCCY 2 cut(s) 128, 674
EcoO65I GGTNACC 1 cut(s) 770
EcoRI GAATTC 1 cut(s) 407
EcoRII CCWGG 1 cut(s) 377
EcoT14I CCWWGG 1 cut(s) 281
ErhI CCWWGG 1 cut(s) 281
FaeI CATG 2 cut(s) 363, 555
FalI AAGNNNNNCTT 4 cut(s) 97, 129, 645, 677
FaqI GGGAC 2 cut(s) 269, 321
FatI CATG 2 cut(s) 359, 551
FauI CCCGC 1 cut(s) 152
FauNDI CATATG 1 cut(s) 85
FbaI TGATCA 1 cut(s) 733
Fnu4HI GCNGC 7 cut(s) 222, 225, 579, 645, 747, 750, 753
FokI GGATG 1 cut(s) 254
Fsp4HI GCNGC 7 cut(s) 222, 225, 579, 645, 747, 750, 753
FspBI CTAG 2 cut(s) 51, 848
GluI GCNGC 7 cut(s) 222, 225, 579, 645, 747, 750, 753
GsaI CCCAGC 1 cut(s) 294
HaeIII GGCC 1 cut(s) 675
Hin1II CATG 2 cut(s) 363, 555
HinfI GANTC 4 cut(s) 382, 444, 699, 804
HphI GGTGA 1 cut(s) 413
Hpy188I TCNGA 5 cut(s) 27, 249, 668, 715, 813
Hpy188III TCNNGA 3 cut(s) 326, 429, 863
HpyAV CCTTC 4 cut(s) 30, 332, 665, 755
HpyCH4V TGCA 5 cut(s) 175, 299, 551, 695, 749
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 752
HpyF3I CTNAG 3 cut(s) 507, 678, 840
Hsp92II CATG 2 cut(s) 363, 555
Ksp22I TGATCA 1 cut(s) 733
Kzo9I GATC 4 cut(s) 538, 569, 658, 733
LguI GCTCTTC 1 cut(s) 115
Lsp1109I GCAGC 7 cut(s) 208, 211, 565, 631, 733, 739, 761
LweI GCATC 2 cut(s) 232, 811
MaeI CTAG 2 cut(s) 51, 848
MaeIII GTNAC 3 cut(s) 401, 770, 873
MalI GATC 4 cut(s) 540, 571, 660, 735
MboI GATC 4 cut(s) 538, 569, 658, 733
MboII GAAGA 6 cut(s) 102, 105, 200, 662, 665, 851
MflI RGATCY 2 cut(s) 569, 658
MhlI GDGCHC 1 cut(s) 50
MluCI AATT 4 cut(s) 77, 300, 407, 521
MlyI GAGTC 1 cut(s) 813
MmeI TCCRAC 3 cut(s) 397, 707, 781
MnlI CCTC 7 cut(s) 142, 349, 552, 580, 649, 687, 731
MroXI GAANNNNTTC 1 cut(s) 424
MseI TTAA 3 cut(s) 267, 275, 687
MspA1I CMGCKG 1 cut(s) 752
MspR9I CCNGG 1 cut(s) 379
Mva1269I GAATGC 1 cut(s) 177
MvaI CCWGG 1 cut(s) 379
MwoI GCNNNNNNNGC 2 cut(s) 296, 752
NdeI CATATG 1 cut(s) 85
NdeII GATC 4 cut(s) 538, 569, 658, 733
NlaIII CATG 2 cut(s) 363, 555
NlaIV GGNNCC 1 cut(s) 130
NmuCI GTSAC 2 cut(s) 401, 873
NspI RCATGY 1 cut(s) 555
PaeI GCATGC 1 cut(s) 555
PciSI GCTCTTC 1 cut(s) 115
PctI GAATGC 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 424
PfeI GAWTC 3 cut(s) 382, 444, 699
PkrI GCNGC 7 cut(s) 223, 226, 580, 646, 748, 751, 754
PleI GAGTC 1 cut(s) 812
PpsI GAGTC 1 cut(s) 812
PpuMI RGGWCCY 1 cut(s) 128
Psp5II RGGWCCY 1 cut(s) 128
Psp6I CCWGG 1 cut(s) 377
PspEI GGTNACC 1 cut(s) 770
PspFI CCCAGC 1 cut(s) 290
PspGI CCWGG 1 cut(s) 377
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 2 cut(s) 128, 674
PspPPI RGGWCCY 1 cut(s) 128
PstI CTGCAG 1 cut(s) 751
PsuI RGATCY 2 cut(s) 569, 658
PvuII CAGCTG 1 cut(s) 752
RsaI GTAC 1 cut(s) 596
RsaNI GTAC 1 cut(s) 595
SapI GCTCTTC 1 cut(s) 115
SaqAI TTAA 3 cut(s) 267, 275, 687
SatI GCNGC 7 cut(s) 222, 225, 579, 645, 747, 750, 753
Sau3AI GATC 4 cut(s) 538, 569, 658, 733
Sau96I GGNCC 2 cut(s) 128, 674
SchI GAGTC 1 cut(s) 813
ScrFI CCNGG 1 cut(s) 379
SduI GDGCHC 1 cut(s) 50
SfaNI GCATC 2 cut(s) 232, 811
SfcI CTRYAG 4 cut(s) 342, 573, 607, 747
SinI GGWCC 1 cut(s) 128
SmlI CTYRAG 1 cut(s) 103
SmoI CTYRAG 1 cut(s) 103
SpeI ACTAGT 1 cut(s) 847
SphI GCATGC 1 cut(s) 555
Sse9I AATT 4 cut(s) 77, 300, 407, 521
SsiI CCGC 1 cut(s) 145
SspI AATATT 1 cut(s) 390
SspMI CTAG 2 cut(s) 51, 848
StyD4I CCNGG 1 cut(s) 377
StyI CCWWGG 1 cut(s) 281
TaqI TCGA 2 cut(s) 195, 411
TasI AATT 4 cut(s) 77, 300, 407, 521
TatI WGTACW 1 cut(s) 594
TfiI GAWTC 3 cut(s) 382, 444, 699
Tru1I TTAA 3 cut(s) 267, 275, 687
Tru9I TTAA 3 cut(s) 267, 275, 687
TseFI GTSAC 2 cut(s) 401, 873
TseI GCWGC 7 cut(s) 221, 224, 578, 644, 746, 749, 752
Tsp45I GTSAC 2 cut(s) 401, 873
TspDTI ATGAA 3 cut(s) 102, 201, 842
TspGWI ACGGA 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 128
XapI RAATTY 2 cut(s) 407, 521
XceI RCATGY 1 cut(s) 555
XcmI CCANNNNNNNNNTGG 1 cut(s) 436
XmnI GAANNNNTTC 1 cut(s) 424
XspI CTAG 2 cut(s) 51, 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.