MD06G1224500.v1.1

Rhodanese-like domain-containing protein 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002465
Physical Location & Seq
Reverse (-)
35524490 .. 35527774
3285 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1224500.v1.1.491

Sequence Viewer

Length: 891 bp
ATGGAAGCTCTGGGATTTCCTTCTCTCAACACTCATAGCATTTTGTGCTCCAGTTCTCAGAAACGAAGAGCCACTAAACCACCATGTCAAAGAACATATTTTTCAAGCATTTCACCTCCATCTTCCCTAAAACTCACACCCTCTTACAGAAGTTACAATTTTCGAAGGAGTGCCACTCGAATGCAAGCTGACAGTGAAGATTACGAGTTGAAGCAAATGAGGGATATGGCTGCTGCCAAAAAGAGATGGGAGGCTCTGGTTAGGGAAGGAAAGGTTAAAGCTTTAACACCAAGGGAAGCCGGGTATGCAATTCAGCTATCTAACAAAACCCTTCTCGATGTTCGTCCCTCTACAGAACACAAAAAGGCATGGGTTAAAGGATCAACCTGGATTCCGATATTTGACGTTGGCAATGAATTCGACGCTGGAACTCTTTCCAGAAAGTTCGTGAATTTCACAATGGGGGGTTGGTGGAGTGGCGTTCCTATGTTGTCATATAACAGCCAGTTCGTGGCCAAAGTTAGTGAGAAGTTTCCAGAAGATACAGATCTTATCGTTGCATGTCAGAAGGGATTGAGATCTCTGGCTGCTTGCGAGCAGCTGTACAATGCAGGCTACAAAAACCTTTTCTGGGTTCAAGGAGGTTTAGAGGCTGCTGAAGAAGAGGATCTTGTCAGAGAAGGACCTCAGCCATTTAAGTTTGCTGGAATTGGTGGGGTTTCAGAATTCCTCGGTTGGACAGATCAGCAAAGAGCTGCAGGTGCCAAAGAAGGTTGGAGTTACCGATTAGTGTTCTCTGCCCGGCTGGTCGGAGTCATTGTCCTTGCCGATGCCTTGTTTCTTGGTGCTCAGCAATTCGGACATTATCTTCAGGATATAAGGTCGCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

297

Amino Acids

33.17

Weight (kDa)

9.25

Isoelectric Point (pI)

48.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 108 - 219 1.9e-09 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 749
Acc36I ACCTGC 1 cut(s) 749
AccB1I GGYRCC 1 cut(s) 761
AccB7I CCANNNNNTGG 1 cut(s) 511
AclWI GGATC 2 cut(s) 388, 675
AcoI YGGCCR 1 cut(s) 513
AcsI RAATTY 3 cut(s) 416, 451, 725
AcuI CTGAAG 2 cut(s) 678, 854
AfaI GTAC 1 cut(s) 605
AfiI CCNNNNNNNGG 2 cut(s) 511, 631
AgsI TTSAA 3 cut(s) 105, 211, 638
AjnI CCWGG 1 cut(s) 386
AluBI AGCT 6 cut(s) 8, 188, 281, 316, 601, 755
AluI AGCT 6 cut(s) 8, 188, 281, 316, 601, 755
Alw21I GWGCWC 2 cut(s) 50, 850
AlwI GGATC 2 cut(s) 388, 675
AoxI GGCC 1 cut(s) 513
ApeKI GCWGC 6 cut(s) 230, 233, 587, 598, 653, 755
ApoI RAATTY 3 cut(s) 416, 451, 725
Asp700I GAANNNNTTC 1 cut(s) 433
AspS9I GGNCC 1 cut(s) 683
AsuC2I CCSGG 2 cut(s) 301, 802
AsuHPI GGTGA 1 cut(s) 105
AsuII TTCGAA 1 cut(s) 163
AvaII GGWCC 1 cut(s) 683
BalI TGGCCA 1 cut(s) 515
BanI GGYRCC 1 cut(s) 761
Bbv12I GWGCWC 2 cut(s) 50, 850
BbvCI CCTCAGC 1 cut(s) 687
BbvI GCAGC 6 cut(s) 217, 220, 574, 610, 640, 742
BccI CCATC 2 cut(s) 127, 240
BcgI CGANNNNNNTGC 2 cut(s) 400, 434
BciT130I CCWGG 1 cut(s) 388
BcnI CCSGG 2 cut(s) 301, 802
BfmI CTRYAG 2 cut(s) 351, 756
BfuAI ACCTGC 1 cut(s) 749
BglII AGATCT 2 cut(s) 547, 578
BisI GCNGC 6 cut(s) 231, 234, 588, 599, 654, 756
BlpI GCTNAGC 1 cut(s) 849
BlsI GCNGC 6 cut(s) 232, 235, 589, 600, 655, 757
Bme1390I CCNGG 3 cut(s) 301, 388, 802
Bme18I GGWCC 1 cut(s) 683
BmgT120I GGNCC 1 cut(s) 683
BmiI GGNNCC 1 cut(s) 763
BmrFI CCNGG 3 cut(s) 301, 388, 802
BmsI GCATC 1 cut(s) 820
BplI GAGNNNNNCTC 2 cut(s) 160, 192
BpmI CTGGAG 1 cut(s) 34
Bpu10I CCTNAGC 1 cut(s) 687
Bpu1102I GCTNAGC 1 cut(s) 849
Bpu14I TTCGAA 1 cut(s) 163
BpuMI CCSGG 2 cut(s) 301, 802
BsaBI GATNNNNATC 2 cut(s) 546, 577
BsaJI CCNNGG 2 cut(s) 290, 730
BsaXI ACNNNNNCTCC 2 cut(s) 804, 834
Bsc4I CCNNNNNNNGG 2 cut(s) 511, 631
Bse1I ACTGG 2 cut(s) 51, 505
Bse3DI GCAATG 1 cut(s) 418
Bse8I GATNNNNATC 2 cut(s) 546, 577
BseBI CCWGG 1 cut(s) 388
BseDI CCNNGG 2 cut(s) 290, 730
BseJI GATNNNNATC 2 cut(s) 546, 577
BseLI CCNNNNNNNGG 2 cut(s) 511, 631
BseMI GCAATG 1 cut(s) 418
BseMII CTCAG 3 cut(s) 71, 701, 863
BseNI ACTGG 2 cut(s) 51, 505
BseXI GCAGC 6 cut(s) 217, 220, 574, 610, 640, 742
BshFI GGCC 1 cut(s) 515
BshNI GGYRCC 1 cut(s) 761
BsiHKAI GWGCWC 2 cut(s) 50, 850
BsiSI CCGG 2 cut(s) 300, 802
BslFI GGGAC 1 cut(s) 330
BslI CCNNNNNNNGG 2 cut(s) 511, 631
BsmFI GGGAC 1 cut(s) 330
BsmI GAATGC 1 cut(s) 186
BsnI GGCC 1 cut(s) 515
Bsp119I TTCGAA 1 cut(s) 163
Bsp1286I GDGCHC 2 cut(s) 50, 850
Bsp1407I TGTACA 1 cut(s) 603
Bsp143I GATC 5 cut(s) 380, 547, 578, 667, 742
Bsp1720I GCTNAGC 1 cut(s) 849
BspANI GGCC 1 cut(s) 515
BspCNI CTCAG 3 cut(s) 70, 700, 862
BspLI GGNNCC 1 cut(s) 763
BspMAI CTGCAG 1 cut(s) 760
BspMI ACCTGC 1 cut(s) 749
BspPI GGATC 2 cut(s) 388, 675
BspQI GCTCTTC 1 cut(s) 61
BspT104I TTCGAA 1 cut(s) 163
BspT107I GGYRCC 1 cut(s) 761
BsrDI GCAATG 1 cut(s) 418
BsrGI TGTACA 1 cut(s) 603
BsrI ACTGG 2 cut(s) 51, 505
BssECI CCNNGG 2 cut(s) 290, 730
BssMI GATC 5 cut(s) 380, 547, 578, 667, 742
BssT1I CCWWGG 1 cut(s) 290
Bst2UI CCWGG 1 cut(s) 388
Bst4CI ACNGT 1 cut(s) 194
Bst6I CTCTTC 2 cut(s) 61, 657
BstAPI GCANNNNNTGC 1 cut(s) 45
BstAUI TGTACA 1 cut(s) 603
BstBI TTCGAA 1 cut(s) 163
BstC8I GCNNGC 4 cut(s) 186, 592, 596, 613
BstDEI CTNAG 3 cut(s) 57, 687, 849
BstKTI GATC 5 cut(s) 383, 550, 581, 670, 745
BstMBI GATC 5 cut(s) 380, 547, 578, 667, 742
BstMWI GCNNNNNNNGC 3 cut(s) 45, 305, 761
BstNI CCWGG 1 cut(s) 388
BstNSI RCATGY 1 cut(s) 564
BstSCI CCNGG 3 cut(s) 299, 386, 800
BstSFI CTRYAG 2 cut(s) 351, 756
BstV1I GCAGC 6 cut(s) 217, 220, 574, 610, 640, 742
BstX2I RGATCY 3 cut(s) 547, 578, 667
BstYI RGATCY 3 cut(s) 547, 578, 667
BsuRI GGCC 1 cut(s) 515
BtsIMutI CAGTG 2 cut(s) 199, 886
BveI ACCTGC 1 cut(s) 749
Cac8I GCNNGC 4 cut(s) 186, 592, 596, 613
Cfr13I GGNCC 1 cut(s) 683
CseI GACGC 1 cut(s) 431
Csp6I GTAC 1 cut(s) 604
CviAII CATG 3 cut(s) 84, 369, 561
CviQI GTAC 1 cut(s) 604
DdeI CTNAG 3 cut(s) 57, 687, 849
DpnI GATC 5 cut(s) 382, 549, 580, 669, 744
DpnII GATC 5 cut(s) 380, 547, 578, 667, 742
EaeI YGGCCR 1 cut(s) 513
Eam1104I CTCTTC 2 cut(s) 61, 657
EarI CTCTTC 2 cut(s) 61, 657
Eco130I CCWWGG 1 cut(s) 290
Eco47I GGWCC 1 cut(s) 683
Eco57I CTGAAG 2 cut(s) 678, 854
EcoO109I RGGNCCY 1 cut(s) 683
EcoRI GAATTC 2 cut(s) 416, 725
EcoRII CCWGG 1 cut(s) 386
EcoT14I CCWWGG 1 cut(s) 290
ErhI CCWWGG 1 cut(s) 290
FaeI CATG 3 cut(s) 87, 372, 564
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FaqI GGGAC 1 cut(s) 330
FatI CATG 3 cut(s) 83, 368, 560
Fnu4HI GCNGC 6 cut(s) 231, 234, 588, 599, 654, 756
Fsp4HI GCNGC 6 cut(s) 231, 234, 588, 599, 654, 756
GluI GCNGC 6 cut(s) 231, 234, 588, 599, 654, 756
GsuI CTGGAG 1 cut(s) 34
HaeIII GGCC 1 cut(s) 515
HapII CCGG 2 cut(s) 300, 802
HgaI GACGC 1 cut(s) 431
Hin1II CATG 3 cut(s) 87, 372, 564
HindIII AAGCTT 1 cut(s) 279
HinfI GANTC 2 cut(s) 391, 813
HpaII CCGG 2 cut(s) 300, 802
HphI GGTGA 1 cut(s) 105
Hpy188I TCNGA 7 cut(s) 60, 396, 567, 677, 724, 812, 860
Hpy188III TCNNGA 5 cut(s) 335, 438, 448, 536, 872
Hpy99I CGWCG 1 cut(s) 425
HpyAV CCTTC 7 cut(s) 30, 159, 260, 341, 562, 674, 764
HpyCH4III ACNGT 1 cut(s) 194
HpyCH4IV ACGT 1 cut(s) 405
HpyCH4V TGCA 5 cut(s) 184, 308, 560, 611, 758
HpyF10VI GCNNNNNNNGC 3 cut(s) 45, 305, 761
HpyF3I CTNAG 3 cut(s) 57, 687, 849
HpySE526I ACGT 1 cut(s) 405
Hsp92II CATG 3 cut(s) 87, 372, 564
Kzo9I GATC 5 cut(s) 380, 547, 578, 667, 742
LguI GCTCTTC 1 cut(s) 61
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 6 cut(s) 217, 220, 574, 610, 640, 742
LweI GCATC 1 cut(s) 820
MaeII ACGT 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 152, 779
MalI GATC 5 cut(s) 382, 549, 580, 669, 744
MboI GATC 5 cut(s) 380, 547, 578, 667, 742
MboII GAAGA 7 cut(s) 78, 114, 209, 551, 671, 674, 860
MflI RGATCY 3 cut(s) 547, 578, 667
MhlI GDGCHC 2 cut(s) 50, 850
MlsI TGGCCA 1 cut(s) 515
MluCI AATT 7 cut(s) 157, 309, 416, 451, 708, 725, 854
MluNI TGGCCA 1 cut(s) 515
MlyI GAGTC 1 cut(s) 822
MmeI TCCRAC 3 cut(s) 716, 755, 790
Mox20I TGGCCA 1 cut(s) 515
MroXI GAANNNNTTC 1 cut(s) 433
MscI TGGCCA 1 cut(s) 515
MseI TTAA 4 cut(s) 276, 284, 375, 696
MslI CAYNNNNRTG 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 515
MspA1I CMGCKG 1 cut(s) 601
MspI CCGG 2 cut(s) 300, 802
MspR9I CCNGG 3 cut(s) 301, 388, 802
Mva1269I GAATGC 1 cut(s) 186
MvaI CCWGG 1 cut(s) 388
MwoI GCNNNNNNNGC 3 cut(s) 45, 305, 761
NciI CCSGG 2 cut(s) 301, 802
NdeII GATC 5 cut(s) 380, 547, 578, 667, 742
NlaIII CATG 3 cut(s) 87, 372, 564
NlaIV GGNNCC 1 cut(s) 763
NspI RCATGY 1 cut(s) 564
NspV TTCGAA 1 cut(s) 163
PaqCI CACCTGC 1 cut(s) 749
PciSI GCTCTTC 1 cut(s) 61
PctI GAATGC 1 cut(s) 186
PdmI GAANNNNTTC 1 cut(s) 433
PfeI GAWTC 1 cut(s) 391
PflMI CCANNNNNTGG 1 cut(s) 511
PkrI GCNGC 6 cut(s) 232, 235, 589, 600, 655, 757
PleI GAGTC 1 cut(s) 821
PpsI GAGTC 1 cut(s) 821
PpuMI RGGWCCY 1 cut(s) 683
Psp5II RGGWCCY 1 cut(s) 683
Psp6I CCWGG 1 cut(s) 386
PspGI CCWGG 1 cut(s) 386
PspN4I GGNNCC 1 cut(s) 763
PspPI GGNCC 1 cut(s) 683
PspPPI RGGWCCY 1 cut(s) 683
PstI CTGCAG 1 cut(s) 760
PsuI RGATCY 3 cut(s) 547, 578, 667
PvuII CAGCTG 1 cut(s) 601
RsaI GTAC 1 cut(s) 605
RsaNI GTAC 1 cut(s) 604
RseI CAYNNNNRTG 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 61
SaqAI TTAA 4 cut(s) 276, 284, 375, 696
SatI GCNGC 6 cut(s) 231, 234, 588, 599, 654, 756
Sau3AI GATC 5 cut(s) 380, 547, 578, 667, 742
Sau96I GGNCC 1 cut(s) 683
SchI GAGTC 1 cut(s) 822
ScrFI CCNGG 3 cut(s) 301, 388, 802
SduI GDGCHC 2 cut(s) 50, 850
SfaNI GCATC 1 cut(s) 820
SfcI CTRYAG 2 cut(s) 351, 756
SfuI TTCGAA 1 cut(s) 163
SinI GGWCC 1 cut(s) 683
SmiMI CAYNNNNRTG 1 cut(s) 179
Sse9I AATT 7 cut(s) 157, 309, 416, 451, 708, 725, 854
StyD4I CCNGG 3 cut(s) 299, 386, 800
StyI CCWWGG 1 cut(s) 290
TaaI ACNGT 1 cut(s) 194
TaiI ACGT 1 cut(s) 408
TaqI TCGA 4 cut(s) 163, 178, 336, 420
TasI AATT 7 cut(s) 157, 309, 416, 451, 708, 725, 854
TatI WGTACW 1 cut(s) 603
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 4 cut(s) 276, 284, 375, 696
Tru9I TTAA 4 cut(s) 276, 284, 375, 696
TscAI CASTG 1 cut(s) 199
TseI GCWGC 6 cut(s) 230, 233, 587, 598, 653, 755
TspDTI ATGAA 1 cut(s) 429
TspRI CASTG 1 cut(s) 199
Van91I CCANNNNNTGG 1 cut(s) 511
VpaK11BI GGWCC 1 cut(s) 683
XapI RAATTY 3 cut(s) 416, 451, 725
XceI RCATGY 1 cut(s) 564
XmnI GAANNNNTTC 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.