FvH4_5g06601

Rhodanese-like domain-containing protein 11

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002465
Physical Location & Seq
Reverse (-)
3891848 .. 3896306
4459 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06601.t1

Sequence Viewer

Length: 876 bp
ATGGAAGCTGTTGGATTTCCTTCTCTCAATACTCACACCATTTCGTGCTCTAGTTTTCTGAAACGAACACCCACCAAATTACCATATCAAACAAACCATGTTTCAACTTCAAGACCAAGAACACCCATATACAGAACCCACCAAATTCGGAGGAGTGCTACACGAATGCAAGCTGACAATGAAGACTTCGAGTTGAAGCAAATGAGAGACATGGCTGCTGCCAAGAAGAGATGGGATGCTCTGATTAGGGATGGAAAGGTTAAACCTTTAACGCCAAGGGAAGCTGGGTATGCAATTCAGCTATCTAACAAAACCCTTCTTGATGTCCGTCCCTCTACAGAACACAAAAAGGCATGGGTTAAAGGCTCAACTTGGATTCCAATATTTGATGTTGGTGATGAATTCGATGCTGGAACTCTTTCCAGAAAGATCATGGGCTTCACTATGGGGGGTTGGTGGAGTGGCGTGCCTACATTGTCGTATAACAACCAGTTCTTGTCTAAGGTTGATGAGAAATTTCCCAAGGATACAGATCTTATCGTTGCATGCCAGAAGGGATTGAGATCTATAGCTGCTTGTGAGGTGCTGTACAATGCTGGCTACAGAAACCTTTTCTGGGTTCAGGGGGGTCTAGAGGCTGCTGAAGAAGAGGATCTTGTCAGAGAAGGCCCTCAGCCATTTAAGCTTGCAGGAATTGGCGGGGTATCAGAGTTCCTTGGTTGGACTGATCAACAAAGAGCTGCAGCTGCTAAAGAAGGGTGGGGTTACCGATTTATGTTCTCTGCCCGTCTGGTTGGAATCTTTCTCATTGCTGATGCCTTATTTCTTGGTGGTCAGCAACTAGTTCATTATCTTCAGGATATAAGGTCACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0009507 GO:0009536 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rhodanese

Protein Analysis

292

Amino Acids

32.8

Weight (kDa)

9.26

Isoelectric Point (pI)

36.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rhodanese PF00581 103 - 214 3.1e-09 Rhodanese-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 699
AclWI GGATC 1 cut(s) 660
AcsI RAATTY 3 cut(s) 144, 401, 515
AcuI CTGAAG 2 cut(s) 663, 839
AfaI GTAC 1 cut(s) 590
AfiI CCNNNNNNNGG 1 cut(s) 616
AgsI TTSAA 3 cut(s) 105, 111, 196
AhlI ACTAGT 1 cut(s) 841
AluBI AGCT 8 cut(s) 8, 173, 284, 301, 572, 685, 740, 746
AluI AGCT 8 cut(s) 8, 173, 284, 301, 572, 685, 740, 746
Alw21I GWGCWC 1 cut(s) 50
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 1 cut(s) 660
AoxI GGCC 1 cut(s) 667
ApeKI GCWGC 7 cut(s) 215, 218, 572, 638, 740, 743, 746
ApoI RAATTY 3 cut(s) 144, 401, 515
Asp700I GAANNNNTTC 1 cut(s) 418
AspS9I GGNCC 1 cut(s) 668
AsuHPI GGTGA 1 cut(s) 407
BbsI GAAGAC 1 cut(s) 189
Bbv12I GWGCWC 1 cut(s) 50
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 7 cut(s) 202, 205, 559, 625, 727, 733, 755
BccI CCATC 2 cut(s) 225, 245
BciVI GTATCC 1 cut(s) 520
BclI TGATCA 1 cut(s) 727
BcoDI GTCTC 1 cut(s) 201
BcuI ACTAGT 1 cut(s) 841
BfaI CTAG 3 cut(s) 51, 632, 842
BfmI CTRYAG 4 cut(s) 336, 567, 601, 741
BfuI GTATCC 1 cut(s) 520
BglII AGATCT 2 cut(s) 532, 563
BisI GCNGC 7 cut(s) 216, 219, 573, 639, 741, 744, 747
BlsI GCNGC 7 cut(s) 217, 220, 574, 640, 742, 745, 748
BmgT120I GGNCC 1 cut(s) 668
BmsI GCATC 3 cut(s) 226, 397, 805
BpiI GAAGAC 1 cut(s) 189
Bpu10I CCTNAGC 1 cut(s) 672
BsaBI GATNNNNATC 2 cut(s) 531, 562
BsaJI CCNNGG 3 cut(s) 275, 522, 715
Bsc4I CCNNNNNNNGG 1 cut(s) 616
Bse1I ACTGG 1 cut(s) 490
Bse3DI GCAATG 1 cut(s) 807
Bse8I GATNNNNATC 2 cut(s) 531, 562
BseDI CCNNGG 3 cut(s) 275, 522, 715
BseGI GGATG 2 cut(s) 241, 256
BseJI GATNNNNATC 2 cut(s) 531, 562
BseLI CCNNNNNNNGG 1 cut(s) 616
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 1 cut(s) 686
BseNI ACTGG 1 cut(s) 490
BseRI GAGGAG 1 cut(s) 166
BseXI GCAGC 7 cut(s) 202, 205, 559, 625, 727, 733, 755
BseYI CCCAGC 1 cut(s) 284
BshFI GGCC 1 cut(s) 669
BsiHKAI GWGCWC 1 cut(s) 50
BslFI GGGAC 1 cut(s) 315
BslI CCNNNNNNNGG 1 cut(s) 616
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 315
BsmI GAATGC 1 cut(s) 171
BsnI GGCC 1 cut(s) 669
Bsp1286I GDGCHC 1 cut(s) 50
Bsp1407I TGTACA 1 cut(s) 588
Bsp143I GATC 5 cut(s) 429, 532, 563, 652, 727
BspACI CCGC 1 cut(s) 699
BspANI GGCC 1 cut(s) 669
BspCNI CTCAG 1 cut(s) 685
BspMAI CTGCAG 1 cut(s) 745
BspPI GGATC 1 cut(s) 660
BsrDI GCAATG 1 cut(s) 807
BsrGI TGTACA 1 cut(s) 588
BsrI ACTGG 1 cut(s) 490
BssECI CCNNGG 3 cut(s) 275, 522, 715
BssMI GATC 5 cut(s) 429, 532, 563, 652, 727
BssT1I CCWWGG 3 cut(s) 275, 522, 715
Bst6I CTCTTC 2 cut(s) 221, 642
BstAUI TGTACA 1 cut(s) 588
BstC8I GCNNGC 5 cut(s) 171, 467, 547, 598, 687
BstDEI CTNAG 2 cut(s) 501, 672
BstEII GGTNACC 1 cut(s) 764
BstF5I GGATG 2 cut(s) 241, 256
BstKTI GATC 5 cut(s) 432, 535, 566, 655, 730
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 5 cut(s) 429, 532, 563, 652, 727
BstMWI GCNNNNNNNGC 3 cut(s) 290, 682, 746
BstNSI RCATGY 1 cut(s) 549
BstPI GGTNACC 1 cut(s) 764
BstSFI CTRYAG 4 cut(s) 336, 567, 601, 741
BstV1I GCAGC 7 cut(s) 202, 205, 559, 625, 727, 733, 755
BstV2I GAAGAC 1 cut(s) 189
BstX2I RGATCY 3 cut(s) 532, 563, 652
BstYI RGATCY 3 cut(s) 532, 563, 652
BsuI GTATCC 1 cut(s) 520
BsuRI GGCC 1 cut(s) 669
BtsCI GGATG 2 cut(s) 241, 256
BtsIMutI CAGTG 1 cut(s) 871
Cac8I GCNNGC 5 cut(s) 171, 467, 547, 598, 687
Cfr13I GGNCC 1 cut(s) 668
Csp6I GTAC 1 cut(s) 589
CviAII CATG 5 cut(s) 98, 211, 354, 433, 546
CviQI GTAC 1 cut(s) 589
DdeI CTNAG 2 cut(s) 501, 672
DpnI GATC 5 cut(s) 431, 534, 565, 654, 729
DpnII GATC 5 cut(s) 429, 532, 563, 652, 727
Eam1104I CTCTTC 2 cut(s) 221, 642
EarI CTCTTC 2 cut(s) 221, 642
Eco130I CCWWGG 3 cut(s) 275, 522, 715
Eco57I CTGAAG 2 cut(s) 663, 839
Eco91I GGTNACC 1 cut(s) 764
EcoO109I RGGNCCY 1 cut(s) 668
EcoO65I GGTNACC 1 cut(s) 764
EcoRI GAATTC 1 cut(s) 401
EcoT14I CCWWGG 3 cut(s) 275, 522, 715
ErhI CCWWGG 3 cut(s) 275, 522, 715
FaeI CATG 5 cut(s) 101, 214, 357, 436, 549
FalI AAGNNNNNCTT 2 cut(s) 639, 671
FaqI GGGAC 1 cut(s) 315
FatI CATG 5 cut(s) 97, 210, 353, 432, 545
FauI CCCGC 1 cut(s) 692
FbaI TGATCA 1 cut(s) 727
Fnu4HI GCNGC 7 cut(s) 216, 219, 573, 639, 741, 744, 747
FokI GGATG 2 cut(s) 248, 263
Fsp4HI GCNGC 7 cut(s) 216, 219, 573, 639, 741, 744, 747
FspBI CTAG 3 cut(s) 51, 632, 842
GluI GCNGC 7 cut(s) 216, 219, 573, 639, 741, 744, 747
GsaI CCCAGC 1 cut(s) 288
HaeIII GGCC 1 cut(s) 669
Hin1II CATG 5 cut(s) 101, 214, 357, 436, 549
HindIII AAGCTT 1 cut(s) 683
HinfI GANTC 2 cut(s) 376, 798
HphI GGTGA 1 cut(s) 407
Hpy188I TCNGA 5 cut(s) 60, 150, 243, 662, 709
Hpy188III TCNNGA 5 cut(s) 111, 320, 423, 632, 857
HpyAV CCTTC 5 cut(s) 30, 326, 547, 659, 749
HpyCH4V TGCA 5 cut(s) 169, 293, 545, 689, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 290, 682, 746
HpyF3I CTNAG 2 cut(s) 501, 672
Hsp92II CATG 5 cut(s) 101, 214, 357, 436, 549
Ksp22I TGATCA 1 cut(s) 727
Kzo9I GATC 5 cut(s) 429, 532, 563, 652, 727
Lsp1109I GCAGC 7 cut(s) 202, 205, 559, 625, 727, 733, 755
LweI GCATC 3 cut(s) 226, 397, 805
MaeI CTAG 3 cut(s) 51, 632, 842
MaeIII GTNAC 2 cut(s) 764, 867
MalI GATC 5 cut(s) 431, 534, 565, 654, 729
MboI GATC 5 cut(s) 429, 532, 563, 652, 727
MboII GAAGA 5 cut(s) 194, 238, 656, 659, 845
MflI RGATCY 3 cut(s) 532, 563, 652
MhlI GDGCHC 1 cut(s) 50
MluCI AATT 6 cut(s) 77, 144, 294, 401, 515, 693
MmeI TCCRAC 2 cut(s) 701, 775
MnlI CCTC 6 cut(s) 144, 343, 574, 628, 643, 681
MroXI GAANNNNTTC 1 cut(s) 418
MseI TTAA 4 cut(s) 261, 269, 360, 681
MspA1I CMGCKG 1 cut(s) 746
Mva1269I GAATGC 1 cut(s) 171
MwoI GCNNNNNNNGC 3 cut(s) 290, 682, 746
NdeII GATC 5 cut(s) 429, 532, 563, 652, 727
NlaIII CATG 5 cut(s) 101, 214, 357, 436, 549
NmuCI GTSAC 1 cut(s) 867
NspI RCATGY 1 cut(s) 549
PaeI GCATGC 1 cut(s) 549
PctI GAATGC 1 cut(s) 171
PdmI GAANNNNTTC 1 cut(s) 418
PfeI GAWTC 2 cut(s) 376, 798
PkrI GCNGC 7 cut(s) 217, 220, 574, 640, 742, 745, 748
PspEI GGTNACC 1 cut(s) 764
PspFI CCCAGC 1 cut(s) 284
PspPI GGNCC 1 cut(s) 668
PstI CTGCAG 1 cut(s) 745
PsuI RGATCY 3 cut(s) 532, 563, 652
PvuII CAGCTG 1 cut(s) 746
RsaI GTAC 1 cut(s) 590
RsaNI GTAC 1 cut(s) 589
SaqAI TTAA 4 cut(s) 261, 269, 360, 681
SatI GCNGC 7 cut(s) 216, 219, 573, 639, 741, 744, 747
Sau3AI GATC 5 cut(s) 429, 532, 563, 652, 727
Sau96I GGNCC 1 cut(s) 668
SduI GDGCHC 1 cut(s) 50
SfaNI GCATC 3 cut(s) 226, 397, 805
SfcI CTRYAG 4 cut(s) 336, 567, 601, 741
SpeI ACTAGT 1 cut(s) 841
SphI GCATGC 1 cut(s) 549
Sse9I AATT 6 cut(s) 77, 144, 294, 401, 515, 693
SsiI CCGC 1 cut(s) 699
SspI AATATT 1 cut(s) 384
SspMI CTAG 3 cut(s) 51, 632, 842
StyI CCWWGG 3 cut(s) 275, 522, 715
TaqI TCGA 2 cut(s) 189, 405
TasI AATT 6 cut(s) 77, 144, 294, 401, 515, 693
TatI WGTACW 1 cut(s) 588
TfiI GAWTC 2 cut(s) 376, 798
Tru1I TTAA 4 cut(s) 261, 269, 360, 681
Tru9I TTAA 4 cut(s) 261, 269, 360, 681
TseFI GTSAC 1 cut(s) 867
TseI GCWGC 7 cut(s) 215, 218, 572, 638, 740, 743, 746
Tsp45I GTSAC 1 cut(s) 867
TspDTI ATGAA 3 cut(s) 195, 414, 836
TspGWI ACGGA 1 cut(s) 317
XapI RAATTY 3 cut(s) 144, 401, 515
XbaI TCTAGA 1 cut(s) 631
XceI RCATGY 1 cut(s) 549
XcmI CCANNNNNNNNNTGG 1 cut(s) 430
XmnI GAANNNNTTC 1 cut(s) 418
XspI CTAG 3 cut(s) 51, 632, 842
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.