AT4G27745

Belongs to the yippee family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000903
Physical Location & Seq
Forward (+)
13839842 .. 13841536
1695 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G27745.4

Sequence Viewer

Length: 321 bp
ATGGCTCAGGCAATTGGTCCAAGACTGTATAGTTGCTGCAACTGCAGAAACCATGTTGGACTTCACGATGATATCATCTCTAAGGCTTTTCAGGGAAGAACTGGGCGAGCCTTCCTGTTCTCCCACGCAATGAACATTGTGGTAGGACCTAAAGAAGACCGGAATCTTCTAACTGGTCTACACACCGTGGCTGATATATCTTGTGTTGACTGTAACGAACCATTGGGTTGGAAATACGAGCGAGCTTATGAGACCTCACAGAAGTACAAGGAGGGCAAGTTCATATTCGAAAAGGCTAAGATTGTCAAGGAGGATTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Yippee-Mis18

Protein Analysis

106

Amino Acids

12.08

Weight (kDa)

8.29

Isoelectric Point (pI)

18.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yippee-Mis18 PF03226 10 - 102 4.3e-13 Yippee zinc-binding/DNA-binding /Mis18, centromere assembly
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 178
AdeI CACNNNGTG 1 cut(s) 187
AfaI GTAC 1 cut(s) 266
AloI GAACNNNNNNTCC 2 cut(s) 263, 295
AluBI AGCT 1 cut(s) 245
AluI AGCT 1 cut(s) 245
Alw26I GTCTC 1 cut(s) 245
ApeKI GCWGC 1 cut(s) 36
AspS9I GGNCC 2 cut(s) 17, 146
AsuII TTCGAA 1 cut(s) 288
AvaII GGWCC 2 cut(s) 17, 146
BbsI GAAGAC 1 cut(s) 162
BbvI GCAGC 1 cut(s) 23
BcoDI GTCTC 1 cut(s) 245
BfmI CTRYAG 1 cut(s) 43
BisI GCNGC 1 cut(s) 37
BlsI GCNGC 1 cut(s) 38
Bme18I GGWCC 2 cut(s) 17, 146
BmgT120I GGNCC 2 cut(s) 17, 146
BmrI ACTGGG 1 cut(s) 111
BmuI ACTGGG 1 cut(s) 111
BpiI GAAGAC 1 cut(s) 162
Bpu10I CCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 245
BsaJI CCNNGG 1 cut(s) 186
BsaWI WCCGGW 1 cut(s) 159
BsaXI ACNNNNNCTCC 3 cut(s) 263, 293, 302
Bse1I ACTGG 2 cut(s) 106, 178
Bse3DI GCAATG 1 cut(s) 135
BseDI CCNNGG 1 cut(s) 186
BseMI GCAATG 1 cut(s) 135
BseMII CTCAG 1 cut(s) 20
BseNI ACTGG 2 cut(s) 106, 178
BseXI GCAGC 1 cut(s) 23
BsiSI CCGG 1 cut(s) 160
BsmAI GTCTC 1 cut(s) 245
Bso31I GGTCTC 1 cut(s) 245
Bsp119I TTCGAA 1 cut(s) 288
BspCNI CTCAG 1 cut(s) 19
BspMAI CTGCAG 1 cut(s) 47
BspT104I TTCGAA 1 cut(s) 288
BspTNI GGTCTC 1 cut(s) 245
BsrDI GCAATG 1 cut(s) 135
BsrI ACTGG 2 cut(s) 106, 178
BssECI CCNNGG 1 cut(s) 186
Bst4CI ACNGT 3 cut(s) 27, 187, 212
BstBI TTCGAA 1 cut(s) 288
BstC8I GCNNGC 2 cut(s) 108, 243
BstDEI CTNAG 3 cut(s) 6, 81, 297
BstDSI CCRYGG 1 cut(s) 186
BstMAI GTCTC 1 cut(s) 245
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstSFI CTRYAG 1 cut(s) 43
BstV1I GCAGC 1 cut(s) 23
BstV2I GAAGAC 1 cut(s) 162
BstXI CCANNNNNNTGG 1 cut(s) 228
BtgI CCRYGG 1 cut(s) 186
Cac8I GCNNGC 2 cut(s) 108, 243
Cfr13I GGNCC 2 cut(s) 17, 146
Csp6I GTAC 1 cut(s) 265
CviAII CATG 1 cut(s) 53
CviJI RGCY 6 cut(s) 5, 86, 110, 191, 245, 296
CviKI_1 RGCY 6 cut(s) 5, 86, 110, 191, 245, 296
CviQI GTAC 1 cut(s) 265
DdeI CTNAG 3 cut(s) 6, 81, 297
DraIII CACNNNGTG 1 cut(s) 187
Eco31I GGTCTC 1 cut(s) 245
Eco32I GATATC 1 cut(s) 73
Eco47I GGWCC 2 cut(s) 17, 146
EcoO109I RGGNCCY 1 cut(s) 146
EcoRV GATATC 1 cut(s) 73
FaeI CATG 1 cut(s) 56
FaiI YATR 5 cut(s) 30, 54, 197, 249, 284
FatI CATG 1 cut(s) 52
FblI GTMKAC 1 cut(s) 178
Fnu4HI GCNGC 1 cut(s) 37
Fsp4HI GCNGC 1 cut(s) 37
GluI GCNGC 1 cut(s) 37
HapII CCGG 1 cut(s) 160
Hin1II CATG 1 cut(s) 56
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 1 cut(s) 163
HpaII CCGG 1 cut(s) 160
Hpy166II GTNNAC 2 cut(s) 179, 208
Hpy188III TCNNGA 1 cut(s) 65
Hpy8I GTNNAC 2 cut(s) 179, 208
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 3 cut(s) 27, 187, 212
HpyCH4V TGCA 2 cut(s) 39, 45
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
HpyF3I CTNAG 3 cut(s) 6, 81, 297
Hsp92II CATG 1 cut(s) 56
LpnPI CCDG 5 cut(s) 77, 87, 128, 159, 173
Lsp1109I GCAGC 1 cut(s) 23
MaeIII GTNAC 1 cut(s) 212
MboII GAAGA 3 cut(s) 108, 158, 167
MfeI CAATTG 1 cut(s) 12
MluCI AATT 1 cut(s) 12
MmeI TCCRAC 2 cut(s) 37, 209
MnlI CCTC 3 cut(s) 265, 265, 304
MspI CCGG 1 cut(s) 160
MunI CAATTG 1 cut(s) 12
MwoI GCNNNNNNNGC 1 cut(s) 42
NlaIII CATG 1 cut(s) 56
NspV TTCGAA 1 cut(s) 288
PfeI GAWTC 1 cut(s) 163
PkrI GCNGC 1 cut(s) 38
PpuMI RGGWCCY 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 146
PspPI GGNCC 2 cut(s) 17, 146
PspPPI RGGWCCY 1 cut(s) 146
PstI CTGCAG 1 cut(s) 47
RsaI GTAC 1 cut(s) 266
RsaNI GTAC 1 cut(s) 265
SatI GCNGC 1 cut(s) 37
Sau96I GGNCC 2 cut(s) 17, 146
SetI ASST 3 cut(s) 151, 247, 257
SfcI CTRYAG 1 cut(s) 43
SfuI TTCGAA 1 cut(s) 288
SinI GGWCC 2 cut(s) 17, 146
Sse9I AATT 1 cut(s) 12
TaaI ACNGT 3 cut(s) 27, 187, 212
TaqI TCGA 1 cut(s) 288
TasI AATT 1 cut(s) 12
TatI WGTACW 1 cut(s) 264
TfiI GAWTC 1 cut(s) 163
TseI GCWGC 1 cut(s) 36
TspDTI ATGAA 2 cut(s) 146, 271
VpaK11BI GGWCC 2 cut(s) 17, 146
XmiI GTMKAC 1 cut(s) 178
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.