Belongs to the yippee family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 165 |
| AcsI | RAATTY | 2 cut(s) | 278, 293 |
| AfaI | GTAC | 2 cut(s) | 198, 266 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 106 |
| AgsI | TTSAA | 1 cut(s) | 290 |
| AleI | CACNNNNGTG | 1 cut(s) | 204 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 267, 299 |
| AluBI | AGCT | 2 cut(s) | 14, 86 |
| AluI | AGCT | 2 cut(s) | 14, 86 |
| Alw26I | GTCTC | 2 cut(s) | 82, 182 |
| AlwI | GGATC | 1 cut(s) | 165 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 191 |
| AoxI | GGCC | 3 cut(s) | 17, 108, 146 |
| ApoI | RAATTY | 2 cut(s) | 278, 293 |
| AspS9I | GGNCC | 3 cut(s) | 17, 108, 146 |
| BceAI | ACGGC | 1 cut(s) | 84 |
| BclI | TGATCA | 1 cut(s) | 168 |
| BcoDI | GTCTC | 2 cut(s) | 82, 182 |
| BmgT120I | GGNCC | 3 cut(s) | 17, 108, 146 |
| BmsI | GCATC | 1 cut(s) | 172 |
| BsaBI | GATNNNNATC | 1 cut(s) | 162 |
| BsaI | GGTCTC | 1 cut(s) | 182 |
| BsaJI | CCNNGG | 2 cut(s) | 20, 100 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 106 |
| Bse8I | GATNNNNATC | 1 cut(s) | 162 |
| BseDI | CCNNGG | 2 cut(s) | 20, 100 |
| BseJI | GATNNNNATC | 1 cut(s) | 162 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 106 |
| BseYI | CCCAGC | 1 cut(s) | 14 |
| BshFI | GGCC | 3 cut(s) | 19, 110, 148 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 106 |
| BsmAI | GTCTC | 2 cut(s) | 82, 182 |
| BsnI | GGCC | 3 cut(s) | 19, 110, 148 |
| Bso31I | GGTCTC | 1 cut(s) | 182 |
| Bsp1407I | TGTACA | 1 cut(s) | 196 |
| Bsp143I | GATC | 3 cut(s) | 157, 168, 309 |
| BspANI | GGCC | 3 cut(s) | 19, 110, 148 |
| BspHI | TCATGA | 1 cut(s) | 64 |
| BspPI | GGATC | 1 cut(s) | 165 |
| BspTNI | GGTCTC | 1 cut(s) | 182 |
| BsrGI | TGTACA | 1 cut(s) | 196 |
| BssECI | CCNNGG | 2 cut(s) | 20, 100 |
| BssMI | GATC | 3 cut(s) | 157, 168, 309 |
| BssT1I | CCWWGG | 1 cut(s) | 20 |
| Bst4CI | ACNGT | 1 cut(s) | 187 |
| BstAUI | TGTACA | 1 cut(s) | 196 |
| BstC8I | GCNNGC | 1 cut(s) | 97 |
| BstDSI | CCRYGG | 1 cut(s) | 100 |
| BstKTI | GATC | 3 cut(s) | 160, 171, 312 |
| BstMAI | GTCTC | 2 cut(s) | 82, 182 |
| BstMBI | GATC | 3 cut(s) | 157, 168, 309 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 11, 42, 92 |
| BsuRI | GGCC | 3 cut(s) | 19, 110, 148 |
| BtgI | CCRYGG | 1 cut(s) | 100 |
| BtsI | GCAGTG | 1 cut(s) | 199 |
| BtsIMutI | CAGTG | 1 cut(s) | 199 |
| Cac8I | GCNNGC | 1 cut(s) | 97 |
| CaiI | CAGNNNCTG | 1 cut(s) | 191 |
| CciI | TCATGA | 1 cut(s) | 64 |
| Cfr13I | GGNCC | 3 cut(s) | 17, 108, 146 |
| Csp6I | GTAC | 2 cut(s) | 197, 265 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 117, 152 |
| CviAII | CATG | 3 cut(s) | 53, 65, 125 |
| CviQI | GTAC | 2 cut(s) | 197, 265 |
| DpnI | GATC | 3 cut(s) | 159, 170, 311 |
| DpnII | GATC | 3 cut(s) | 157, 168, 309 |
| Eco130I | CCWWGG | 1 cut(s) | 20 |
| Eco31I | GGTCTC | 1 cut(s) | 182 |
| EcoO109I | RGGNCCY | 2 cut(s) | 108, 146 |
| EcoT14I | CCWWGG | 1 cut(s) | 20 |
| ErhI | CCWWGG | 1 cut(s) | 20 |
| FaeI | CATG | 3 cut(s) | 56, 68, 128 |
| FaiI | YATR | 6 cut(s) | 30, 54, 66, 126, 237, 249 |
| FatI | CATG | 3 cut(s) | 52, 64, 124 |
| FbaI | TGATCA | 1 cut(s) | 168 |
| GsaI | CCCAGC | 1 cut(s) | 18 |
| HaeIII | GGCC | 3 cut(s) | 19, 110, 148 |
| Hin1II | CATG | 3 cut(s) | 56, 68, 128 |
| HindIII | AAGCTT | 1 cut(s) | 84 |
| HinfI | GANTC | 1 cut(s) | 49 |
| Hpy166II | GTNNAC | 1 cut(s) | 199 |
| Hpy188I | TCNGA | 1 cut(s) | 168 |
| Hpy188III | TCNNGA | 2 cut(s) | 65, 287 |
| Hpy8I | GTNNAC | 1 cut(s) | 199 |
| HpyAV | CCTTC | 1 cut(s) | 266 |
| HpyCH4III | ACNGT | 1 cut(s) | 187 |
| HpyCH4IV | ACGT | 1 cut(s) | 135 |
| HpyCH4V | TGCA | 2 cut(s) | 128, 259 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 11, 42, 92 |
| HpySE526I | ACGT | 1 cut(s) | 135 |
| Hsp92II | CATG | 3 cut(s) | 56, 68, 128 |
| Ksp22I | TGATCA | 1 cut(s) | 168 |
| Kzo9I | GATC | 3 cut(s) | 157, 168, 309 |
| LpnPI | CCDG | 5 cut(s) | 59, 77, 91, 159, 214 |
| LweI | GCATC | 1 cut(s) | 172 |
| MaeII | ACGT | 1 cut(s) | 135 |
| MalI | GATC | 3 cut(s) | 159, 170, 311 |
| MboI | GATC | 3 cut(s) | 157, 168, 309 |
| MfeI | CAATTG | 1 cut(s) | 313 |
| MluCI | AATT | 4 cut(s) | 254, 278, 293, 313 |
| MnlI | CCTC | 3 cut(s) | 148, 211, 244 |
| MseI | TTAA | 2 cut(s) | 297, 306 |
| MslI | CAYNNNNRTG | 1 cut(s) | 204 |
| MunI | CAATTG | 1 cut(s) | 313 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 11, 42, 92 |
| NdeII | GATC | 3 cut(s) | 157, 168, 309 |
| NlaIII | CATG | 3 cut(s) | 56, 68, 128 |
| OliI | CACNNNNGTG | 1 cut(s) | 204 |
| PagI | TCATGA | 1 cut(s) | 64 |
| PfeI | GAWTC | 1 cut(s) | 49 |
| PspFI | CCCAGC | 1 cut(s) | 14 |
| PspPI | GGNCC | 3 cut(s) | 17, 108, 146 |
| PstNI | CAGNNNCTG | 1 cut(s) | 191 |
| RsaI | GTAC | 2 cut(s) | 198, 266 |
| RsaNI | GTAC | 2 cut(s) | 197, 265 |
| RseI | CAYNNNNRTG | 1 cut(s) | 204 |
| SaqAI | TTAA | 2 cut(s) | 297, 306 |
| Sau3AI | GATC | 3 cut(s) | 157, 168, 309 |
| Sau96I | GGNCC | 3 cut(s) | 17, 108, 146 |
| SetI | ASST | 5 cut(s) | 16, 88, 138, 178, 222 |
| SfaNI | GCATC | 1 cut(s) | 172 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 204 |
| Sse9I | AATT | 4 cut(s) | 254, 278, 293, 313 |
| StyI | CCWWGG | 1 cut(s) | 20 |
| TaaI | ACNGT | 1 cut(s) | 187 |
| TaiI | ACGT | 1 cut(s) | 138 |
| TasI | AATT | 4 cut(s) | 254, 278, 293, 313 |
| TatI | WGTACW | 2 cut(s) | 196, 264 |
| TfiI | GAWTC | 1 cut(s) | 49 |
| Tru1I | TTAA | 2 cut(s) | 297, 306 |
| Tru9I | TTAA | 2 cut(s) | 297, 306 |
| TscAI | CASTG | 1 cut(s) | 206 |
| TspRI | CASTG | 1 cut(s) | 206 |
| XapI | RAATTY | 2 cut(s) | 278, 293 |