Belongs to the yippee family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 166 |
| AciI | CCGC | 1 cut(s) | 5 |
| AcoI | YGGCCR | 1 cut(s) | 103 |
| AcyI | GRCGYC | 1 cut(s) | 163 |
| AfaI | GTAC | 3 cut(s) | 29, 236, 266 |
| AgeI | ACCGGT | 1 cut(s) | 173 |
| AleI | CACNNNNGTG | 1 cut(s) | 204 |
| AluBI | AGCT | 2 cut(s) | 33, 245 |
| AluI | AGCT | 2 cut(s) | 33, 245 |
| Alw26I | GTCTC | 1 cut(s) | 170 |
| AoxI | GGCC | 2 cut(s) | 17, 103 |
| AsiGI | ACCGGT | 1 cut(s) | 173 |
| AspS9I | GGNCC | 1 cut(s) | 17 |
| AsuHPI | GGTGA | 1 cut(s) | 218 |
| BbsI | GAAGAC | 1 cut(s) | 162 |
| BceAI | ACGGC | 1 cut(s) | 198 |
| BcoDI | GTCTC | 1 cut(s) | 170 |
| BfaI | CTAG | 1 cut(s) | 21 |
| BmgT120I | GGNCC | 1 cut(s) | 17 |
| BmiI | GGNNCC | 1 cut(s) | 18 |
| BoxI | GACNNNNGTC | 1 cut(s) | 162 |
| BpiI | GAAGAC | 1 cut(s) | 162 |
| BsaHI | GRCGYC | 1 cut(s) | 163 |
| BsaWI | WCCGGW | 1 cut(s) | 173 |
| Bse118I | RCCGGY | 1 cut(s) | 173 |
| Bse3DI | GCAATG | 2 cut(s) | 135, 254 |
| BseMI | GCAATG | 2 cut(s) | 135, 254 |
| BshFI | GGCC | 2 cut(s) | 19, 105 |
| BshTI | ACCGGT | 1 cut(s) | 173 |
| BsiSI | CCGG | 1 cut(s) | 174 |
| BsmAI | GTCTC | 1 cut(s) | 170 |
| BsmBI | CGTCTC | 1 cut(s) | 170 |
| BsnI | GGCC | 2 cut(s) | 19, 105 |
| Bsp1407I | TGTACA | 1 cut(s) | 27 |
| BspACI | CCGC | 1 cut(s) | 5 |
| BspANI | GGCC | 2 cut(s) | 19, 105 |
| BspHI | TCATGA | 1 cut(s) | 64 |
| BspLI | GGNNCC | 1 cut(s) | 18 |
| BsrDI | GCAATG | 2 cut(s) | 135, 254 |
| BsrFI | RCCGGY | 1 cut(s) | 173 |
| BsrGI | TGTACA | 1 cut(s) | 27 |
| BssAI | RCCGGY | 1 cut(s) | 173 |
| BssNI | GRCGYC | 1 cut(s) | 163 |
| Bst4CI | ACNGT | 2 cut(s) | 187, 203 |
| BstACI | GRCGYC | 1 cut(s) | 163 |
| BstAUI | TGTACA | 1 cut(s) | 27 |
| BstMAI | GTCTC | 1 cut(s) | 170 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 251 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 162 |
| BstV2I | GAAGAC | 1 cut(s) | 162 |
| BsuRI | GGCC | 2 cut(s) | 19, 105 |
| BtsIMutI | CAGTG | 1 cut(s) | 199 |
| CciI | TCATGA | 1 cut(s) | 64 |
| Cfr10I | RCCGGY | 1 cut(s) | 173 |
| Cfr13I | GGNCC | 1 cut(s) | 17 |
| Csp6I | GTAC | 3 cut(s) | 28, 235, 265 |
| CspAI | ACCGGT | 1 cut(s) | 173 |
| CviAII | CATG | 3 cut(s) | 53, 65, 101 |
| CviJI | RGCY | 7 cut(s) | 19, 33, 86, 105, 110, 228, 245 |
| CviKI_1 | RGCY | 7 cut(s) | 19, 33, 86, 105, 110, 228, 245 |
| CviQI | GTAC | 3 cut(s) | 28, 235, 265 |
| EaeI | YGGCCR | 1 cut(s) | 103 |
| EciI | GGCGGA | 1 cut(s) | 20 |
| Eco32I | GATATC | 2 cut(s) | 73, 196 |
| EcoO109I | RGGNCCY | 1 cut(s) | 17 |
| EcoRV | GATATC | 2 cut(s) | 73, 196 |
| Esp3I | CGTCTC | 1 cut(s) | 170 |
| FaeI | CATG | 3 cut(s) | 56, 68, 104 |
| FaiI | YATR | 6 cut(s) | 54, 66, 102, 183, 249, 284 |
| FatI | CATG | 3 cut(s) | 52, 64, 100 |
| FspBI | CTAG | 1 cut(s) | 21 |
| HaeIII | GGCC | 2 cut(s) | 19, 105 |
| HapII | CCGG | 1 cut(s) | 174 |
| Hin1I | GRCGYC | 1 cut(s) | 163 |
| Hin1II | CATG | 3 cut(s) | 56, 68, 104 |
| HpaII | CCGG | 1 cut(s) | 174 |
| HphI | GGTGA | 1 cut(s) | 218 |
| Hpy188III | TCNNGA | 1 cut(s) | 65 |
| HpyAV | CCTTC | 2 cut(s) | 266, 301 |
| HpyCH4III | ACNGT | 2 cut(s) | 187, 203 |
| HpyCH4IV | ACGT | 1 cut(s) | 163 |
| HpyCH4V | TGCA | 3 cut(s) | 45, 181, 259 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 251 |
| HpySE526I | ACGT | 1 cut(s) | 163 |
| Hsp92I | GRCGYC | 1 cut(s) | 163 |
| Hsp92II | CATG | 3 cut(s) | 56, 68, 104 |
| LpnPI | CCDG | 3 cut(s) | 77, 187, 214 |
| MaeI | CTAG | 1 cut(s) | 21 |
| MaeII | ACGT | 1 cut(s) | 163 |
| MboII | GAAGA | 1 cut(s) | 167 |
| MluCI | AATT | 2 cut(s) | 40, 300 |
| MnlI | CCTC | 2 cut(s) | 211, 244 |
| MseI | TTAA | 1 cut(s) | 170 |
| MslI | CAYNNNNRTG | 1 cut(s) | 204 |
| MspA1I | CMGCKG | 1 cut(s) | 33 |
| MspI | CCGG | 1 cut(s) | 174 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 251 |
| NlaIII | CATG | 3 cut(s) | 56, 68, 104 |
| NlaIV | GGNNCC | 1 cut(s) | 18 |
| NmeAIII | GCCGAG | 1 cut(s) | 131 |
| OliI | CACNNNNGTG | 1 cut(s) | 204 |
| PagI | TCATGA | 1 cut(s) | 64 |
| PinAI | ACCGGT | 1 cut(s) | 173 |
| PshAI | GACNNNNGTC | 1 cut(s) | 162 |
| PspN4I | GGNNCC | 1 cut(s) | 18 |
| PspPI | GGNCC | 1 cut(s) | 17 |
| PvuII | CAGCTG | 1 cut(s) | 33 |
| RsaI | GTAC | 3 cut(s) | 29, 236, 266 |
| RsaNI | GTAC | 3 cut(s) | 28, 235, 265 |
| RseI | CAYNNNNRTG | 1 cut(s) | 204 |
| SaqAI | TTAA | 1 cut(s) | 170 |
| Sau96I | GGNCC | 1 cut(s) | 17 |
| SetI | ASST | 4 cut(s) | 35, 166, 222, 247 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 204 |
| Sse9I | AATT | 2 cut(s) | 40, 300 |
| SsiI | CCGC | 1 cut(s) | 5 |
| SspMI | CTAG | 1 cut(s) | 21 |
| TaaI | ACNGT | 2 cut(s) | 187, 203 |
| TaiI | ACGT | 1 cut(s) | 166 |
| TasI | AATT | 2 cut(s) | 40, 300 |
| TatI | WGTACW | 2 cut(s) | 27, 264 |
| Tru1I | TTAA | 1 cut(s) | 170 |
| Tru9I | TTAA | 1 cut(s) | 170 |
| TscAI | CASTG | 1 cut(s) | 206 |
| TspDTI | ATGAA | 2 cut(s) | 53, 146 |
| TspRI | CASTG | 1 cut(s) | 206 |
| XspI | CTAG | 1 cut(s) | 21 |
| ZraI | GACGTC | 1 cut(s) | 164 |