Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0002125

Physical Location & Seq

Genomic Coordinates Reverse (-)

13851777 .. 13854237

Gene Structure 2461 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G27800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1167 bp

ATGGCGCTTCTGAGGCCGCATCTTCATCGCTTTCACTCCAACACTCTCCGCCACTCAGCCTATCCTTCCGCCGACGCTGGCGGTGGCCTTGTCGTCTATCCCACGTATGGTCGTCACCGTTGCTCCGCGATTGCGATCGACGCTCCGTCGTCTCTGACTGGCGTGACGCCGATTCGTTGGGGTTACACGAGTGTTCAAGGATTCCGAGATGAGATGGAAGACGACATCGTAATCCGTTCTGATGCTGTCGATAGCTTCTCCTACGCAGCGGTCTTCGACGGCCACGCTGGGTCGTCCTCTGTCAAATTCCTCAGGGAAGAGCTGTATAAAGAGTGTGTTGGTGCATTGCAAGCTGGATCGTTGTTGAATGGAGGCGATTTCGCAGCAATCAAGGAAGCTTTGATTAAGGCCTTTGAAAGCGTTGACCGCAATTTGCTCAAATGGCTAGAAGCTAACGGTGACGAAGAAGATGAATCAGGTTCAACAGCCACTGTAATGATCATCAGAAATGATGTTTCCTTCATTGCTCATATTGGTGATTCGTGTGCAGTCTTATCTCGATCTGGGCAAATCGAGGAATTGACTGATTATCACCGTCCATATGGAAGCAGTAGAGCAGCTATTCAGGAAGTGAAAAGGGTCAAAGAAGCAGGTGGATGGATTGTCAATGGAAGGATTTGTGGAGACATAGCTGTATCTCGTGCTTTTGGTGACATCCGGTTTAAGACAAAGAAGAATGATATGTTAAAGAAGGGTGTTGATGAAGGAAGATGGTCCGAGAAGTTTGTTTCCAGAATCGAGTTTAAAGGGGACATGGTTGTGGCGACTCCAGATATCTTCCAAGTACCTCTGACATCTGATGTGGAATTCATTATTTTAGCTTCTGATGGATTATGGGACTACATGAAGAGCTCAGATGTGGTTAGTTATGTTAGGGATCAGCTCCGGAAACATGGGAATGTGCAGCTTGCTTGTGAATCTCTTGCGCAAGTAGCTTTGGACCGGCGATCTCAGGACAACATTAGCATCATTATTGCTGATCTAGGAAGAACAGAGTGGAAGAATCTCCCCGCGCAGCGACAAAACGTAGTGGTTGAACTGGTTCAGGCTGCAACGACTATCGGTTTAGTTACTGTTGGCATATGGATGTCTTCACATCTATCTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006091 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009579 GO:0009767 GO:0009987 GO:0010109 GO:0015979 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019222 GO:0019684 GO:0022900 GO:0031323 GO:0031974 GO:0031981 GO:0042578 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044422 GO:0044424 GO:0044428 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0050789 GO:0050794 GO:0055114 GO:0065007 GO:0070013 GO:0080005

KEGG Pathways

Metabolic & Signaling

ko04010 map04010

Pfam Domains

Protein Families

PP2C

Protein Analysis

388

Amino Acids

42.72

Weight (kDa)

5.73

Isoelectric Point (pI)

45.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	61 - 341	6.1e-55	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002125)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27800 AT4G27800 AT4G27800
fragaria_vesca	FvH4_7g32030 FvH4_7g32030 FvH4_7g32030 FvH4_7g32030
malus_domestica	MD01G1223200.v1.1 MD07G1293900.v1.1
prunus_persica	Prupe.2G313700_v2.0.a1
pyrus_communis	pycom01g23250 pycom07g26670
rosa_chinensis	RchiOBHm_Chr1g0381641 RchiOBHm_Chr3g0453351 RchiOBHm_Chr5g0046991
rosa_laevigata	RLG00000026218
rosa_multiflora	Rmu_sc0001912.1_g000016 Rmu_sc0003181.1_g000017 Rmu_sc0003181.1_g000018 Rmu_sc0010385.1_g000007 Rmu_sc0022590.1_g000002 Rmu_sc0026037.1_g000002 Rmu_sc0034099.1_g000001
rosa_roxburghii	Rroxscaffold_3G00223370 Rroxscaffold_4G00278560
rosa_rugosa	Rorug01G0430100 Rorug01G0430200 Rorug03G0259100 Rorug03G0265500 Rorug04G0148900
rosa_samantha	Rh1AG157900 Rh1AG197200 Rh1AG340200 Rh1AG453300 Rh1BG410400 Rh1CG147500 Rh1CG182200 Rh1CG424900 Rh1DG442100 Rh3AG297400 Rh5BG473100 Rh5CG495700
rosa_wichuraiana	Rw1G039540 Rw6G012220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	641
Acc16I	TGCGCA	1 cut(s)	987
Acc36I	ACCTGC	1 cut(s)	641
AccII	CGCG	2 cut(s)	128, 1073
AccIII	TCCGGA	1 cut(s)	945
AciI	CCGC	8 cut(s)	17, 49, 69, 81, 126, 269, 427, 1071
AclWI	GGATC	2 cut(s)	364, 945
AcoI	YGGCCR	1 cut(s)	280
AcsI	RAATTY	2 cut(s)	305, 866
AcyI	GRCGYC	1 cut(s)	167
AfaI	GTAC	1 cut(s)	846
AfiI	CCNNNNNNNGG	1 cut(s)	107
AgsI	TTSAA	5 cut(s)	197, 367, 416, 483, 1097
AhdI	GACNNNNNGTC	1 cut(s)	145
Alw21I	GWGCWC	1 cut(s)	914
Alw26I	GTCTC	2 cut(s)	156, 678
AlwI	GGATC	2 cut(s)	364, 945
AlwNI	CAGNNNCTG	1 cut(s)	491
Aor13HI	TCCGGA	1 cut(s)	945
AoxI	GGCC	4 cut(s)	14, 85, 280, 408
ApeKI	GCWGC	6 cut(s)	266, 383, 617, 964, 1075, 1109
ApoI	RAATTY	2 cut(s)	305, 866
Asp700I	GAANNNNTTC	1 cut(s)	1101
AspLEI	GCGC	3 cut(s)	7, 988, 1075
AspS9I	GGNCC	2 cut(s)	774, 1000
AsuHPI	GGTGA	5 cut(s)	107, 470, 548, 584, 722
AvaII	GGWCC	2 cut(s)	774, 1000
AxyI	CCTNAGG	1 cut(s)	311
BanII	GRGCYC	1 cut(s)	914
BauI	CACGAG	2 cut(s)	187, 699
BbsI	GAAGAC	3 cut(s)	225, 265, 1143
Bbv12I	GWGCWC	1 cut(s)	914
BbvI	GCAGC	6 cut(s)	278, 395, 629, 976, 1087, 1096
BccI	CCATC	4 cut(s)	208, 651, 765, 881
BceAI	ACGGC	1 cut(s)	295
BcgI	CGANNNNNNTGC	2 cut(s)	8, 42
BclI	TGATCA	1 cut(s)	498
BcoDI	GTCTC	2 cut(s)	156, 678
BfaI	CTAG	2 cut(s)	446, 1043
BfoI	RGCGCY	1 cut(s)	8
BfuAI	ACCTGC	1 cut(s)	641
BisI	GCNGC	7 cut(s)	17, 267, 384, 618, 965, 1076, 1110
BlsI	GCNGC	7 cut(s)	18, 268, 385, 619, 966, 1077, 1111
Bme18I	GGWCC	2 cut(s)	774, 1000
BmeRI	GACNNNNNGTC	1 cut(s)	145
BmgT120I	GGNCC	2 cut(s)	774, 1000
BmsI	GCATC	3 cut(s)	28, 232, 1035
BpiI	GAAGAC	3 cut(s)	225, 265, 1143
BpmI	CTGGAG	1 cut(s)	813
BsaAI	YACGTR	1 cut(s)	105
BsaBI	GATNNNNATC	1 cut(s)	134
BsaHI	GRCGYC	1 cut(s)	167
BsaWI	WCCGGW	2 cut(s)	717, 945
BsaXI	ACNNNNNCTCC	2 cut(s)	107, 137
Bsc4I	CCNNNNNNNGG	1 cut(s)	107
Bse118I	RCCGGY	1 cut(s)	1002
Bse1I	ACTGG	2 cut(s)	163, 1104
Bse21I	CCTNAGG	1 cut(s)	311
Bse3DI	GCAATG	2 cut(s)	344, 522
Bse8I	GATNNNNATC	1 cut(s)	134
BseAI	TCCGGA	1 cut(s)	945
BseGI	GGATG	3 cut(s)	662, 714, 1152
BseJI	GATNNNNATC	1 cut(s)	134
BseLI	CCNNNNNNNGG	1 cut(s)	107
BseMI	GCAATG	2 cut(s)	344, 522
BseMII	CTCAG	4 cut(s)	69, 325, 927, 1025
BseNI	ACTGG	2 cut(s)	163, 1104
BseXI	GCAGC	6 cut(s)	278, 395, 629, 976, 1087, 1096
BseYI	CCCAGC	1 cut(s)	287
BsgI	GTGCAG	2 cut(s)	567, 983
Bsh1236I	CGCG	2 cut(s)	128, 1073
Bsh1285I	CGRYCG	1 cut(s)	138
BshFI	GGCC	4 cut(s)	16, 87, 282, 410
BsiEI	CGRYCG	1 cut(s)	138
BsiHKAI	GWGCWC	1 cut(s)	914
BsiSI	CCGG	3 cut(s)	718, 946, 1003
BslFI	GGGAC	2 cut(s)	824, 911
BslI	CCNNNNNNNGG	1 cut(s)	107
BsmAI	GTCTC	2 cut(s)	156, 678
BsmBI	CGTCTC	1 cut(s)	156
BsmFI	GGGAC	2 cut(s)	824, 911
BsnI	GGCC	4 cut(s)	16, 87, 282, 410
Bsp1286I	GDGCHC	1 cut(s)	914
Bsp13I	TCCGGA	1 cut(s)	945
Bsp143I	GATC	7 cut(s)	135, 356, 498, 560, 937, 1007, 1039
BspACI	CCGC	8 cut(s)	17, 49, 69, 81, 126, 269, 427, 1071
BspANI	GGCC	4 cut(s)	16, 87, 282, 410
BspCNI	CTCAG	4 cut(s)	68, 324, 926, 1024
BspEI	TCCGGA	1 cut(s)	945
BspFNI	CGCG	2 cut(s)	128, 1073
BspMI	ACCTGC	1 cut(s)	641
BspPI	GGATC	2 cut(s)	364, 945
BspQI	GCTCTTC	2 cut(s)	312, 902
BsrDI	GCAATG	2 cut(s)	344, 522
BsrFI	RCCGGY	1 cut(s)	1002
BsrI	ACTGG	2 cut(s)	163, 1104
BssAI	RCCGGY	1 cut(s)	1002
BssMI	GATC	7 cut(s)	135, 356, 498, 560, 937, 1007, 1039
BssNI	GRCGYC	1 cut(s)	167
BssSI	CACGAG	2 cut(s)	187, 699
Bst2BI	CACGAG	2 cut(s)	187, 699
Bst4CI	ACNGT	5 cut(s)	119, 458, 493, 596, 1135
Bst6I	CTCTTC	2 cut(s)	312, 902
BstACI	GRCGYC	1 cut(s)	167
BstBAI	YACGTR	1 cut(s)	105
BstC8I	GCNNGC	3 cut(s)	79, 351, 969
BstDEI	CTNAG	5 cut(s)	11, 55, 311, 913, 1011
BstF5I	GGATG	3 cut(s)	662, 714, 1152
BstFNI	CGCG	2 cut(s)	128, 1073
BstH2I	RGCGCY	1 cut(s)	8
BstHHI	GCGC	3 cut(s)	7, 988, 1075
BstKTI	GATC	7 cut(s)	138, 359, 501, 563, 940, 1010, 1042
BstMAI	GTCTC	2 cut(s)	156, 678
BstMBI	GATC	7 cut(s)	135, 356, 498, 560, 937, 1007, 1039
BstMCI	CGRYCG	1 cut(s)	138
BstMWI	GCNNNNNNNGC	6 cut(s)	13, 140, 350, 426, 442, 992
BstUI	CGCG	2 cut(s)	128, 1073
BstV1I	GCAGC	6 cut(s)	278, 395, 629, 976, 1087, 1096
BstV2I	GAAGAC	3 cut(s)	225, 265, 1143
Bsu36I	CCTNAGG	1 cut(s)	311
BsuRI	GGCC	4 cut(s)	16, 87, 282, 410
BtgZI	GCGATG	1 cut(s)	11
BtsCI	GGATG	3 cut(s)	662, 714, 1152
BtsIMutI	CAGTG	1 cut(s)	489
BveI	ACCTGC	1 cut(s)	641
Cac8I	GCNNGC	3 cut(s)	79, 351, 969
CaiI	CAGNNNCTG	1 cut(s)	491
CfoI	GCGC	3 cut(s)	7, 988, 1075
Cfr10I	RCCGGY	1 cut(s)	1002
Cfr13I	GGNCC	2 cut(s)	774, 1000
CseI	GACGC	3 cut(s)	83, 149, 175
Csp6I	GTAC	1 cut(s)	845
CviAII	CATG	3 cut(s)	814, 904, 953
CviQI	GTAC	1 cut(s)	845
DdeI	CTNAG	5 cut(s)	11, 55, 311, 913, 1011
DpnI	GATC	7 cut(s)	137, 358, 500, 562, 939, 1009, 1041
DpnII	GATC	7 cut(s)	135, 356, 498, 560, 937, 1007, 1039
DraI	TTTAAA	1 cut(s)	805
DriI	GACNNNNNGTC	1 cut(s)	145
EaeI	YGGCCR	1 cut(s)	280
Eam1104I	CTCTTC	2 cut(s)	312, 902
Eam1105I	GACNNNNNGTC	1 cut(s)	145
EarI	CTCTTC	2 cut(s)	312, 902
EciI	GGCGGA	2 cut(s)	38, 58
Ecl136II	GAGCTC	1 cut(s)	912
Eco147I	AGGCCT	1 cut(s)	410
Eco24I	GRGCYC	1 cut(s)	914
Eco32I	GATATC	1 cut(s)	835
Eco47I	GGWCC	2 cut(s)	774, 1000
Eco53kI	GAGCTC	1 cut(s)	912
Eco81I	CCTNAGG	1 cut(s)	311
EcoICRI	GAGCTC	1 cut(s)	912
EcoRI	GAATTC	1 cut(s)	866
EcoRV	GATATC	1 cut(s)	835
EcoT38I	GRGCYC	1 cut(s)	914
Esp3I	CGTCTC	1 cut(s)	156
FaeI	CATG	3 cut(s)	817, 907, 956
FaqI	GGGAC	2 cut(s)	824, 911
FatI	CATG	3 cut(s)	813, 903, 952
FauI	CCCGC	1 cut(s)	1078
FauNDI	CATATG	2 cut(s)	601, 1142
FbaI	TGATCA	1 cut(s)	498
Fnu4HI	GCNGC	7 cut(s)	17, 267, 384, 618, 965, 1076, 1110
FokI	GGATG	3 cut(s)	669, 701, 1159
FriOI	GRGCYC	1 cut(s)	914
Fsp4HI	GCNGC	7 cut(s)	17, 267, 384, 618, 965, 1076, 1110
FspBI	CTAG	2 cut(s)	446, 1043
FspI	TGCGCA	1 cut(s)	987
GlaI	GCGC	3 cut(s)	6, 987, 1074
GluI	GCNGC	7 cut(s)	17, 267, 384, 618, 965, 1076, 1110
GsaI	CCCAGC	1 cut(s)	291
GsuI	CTGGAG	1 cut(s)	813
HaeII	RGCGCY	1 cut(s)	8
HaeIII	GGCC	4 cut(s)	16, 87, 282, 410
HapII	CCGG	3 cut(s)	718, 946, 1003
HgaI	GACGC	3 cut(s)	83, 149, 175
HhaI	GCGC	3 cut(s)	7, 988, 1075
Hin1I	GRCGYC	1 cut(s)	167
Hin1II	CATG	3 cut(s)	817, 907, 956
Hin6I	GCGC	3 cut(s)	5, 986, 1073
HinP1I	GCGC	3 cut(s)	5, 986, 1073
HincII	GTYRAC	1 cut(s)	424
HindII	GTYRAC	1 cut(s)	424
HindIII	AAGCTT	1 cut(s)	396
HinfI	GANTC	8 cut(s)	172, 201, 473, 539, 795, 826, 977, 1063
HpaII	CCGG	3 cut(s)	718, 946, 1003
HphI	GGTGA	5 cut(s)	107, 470, 548, 584, 722
Hpy166II	GTNNAC	1 cut(s)	424
Hpy188III	TCNNGA	6 cut(s)	558, 626, 792, 830, 946, 1013
Hpy8I	GTNNAC	1 cut(s)	424
Hpy99I	CGWCG	4 cut(s)	77, 143, 151, 281
HpyAV	CCTTC	5 cut(s)	75, 529, 666, 745, 758
HpyCH4III	ACNGT	5 cut(s)	119, 458, 493, 596, 1135
HpyCH4IV	ACGT	2 cut(s)	104, 1086
HpyCH4V	TGCA	5 cut(s)	344, 349, 548, 964, 1112
HpyF10VI	GCNNNNNNNGC	6 cut(s)	13, 140, 350, 426, 442, 992
HpyF3I	CTNAG	5 cut(s)	11, 55, 311, 913, 1011
HpySE526I	ACGT	2 cut(s)	104, 1086
Hsp92I	GRCGYC	1 cut(s)	167
Hsp92II	CATG	3 cut(s)	817, 907, 956
HspAI	GCGC	3 cut(s)	5, 986, 1073
Kpn2I	TCCGGA	1 cut(s)	945
Ksp22I	TGATCA	1 cut(s)	498
Kzo9I	GATC	7 cut(s)	135, 356, 498, 560, 937, 1007, 1039
LguI	GCTCTTC	2 cut(s)	312, 902
LmnI	GCTCC	3 cut(s)	128, 148, 948
Lsp1109I	GCAGC	6 cut(s)	278, 395, 629, 976, 1087, 1096
LweI	GCATC	3 cut(s)	28, 232, 1035
MaeI	CTAG	2 cut(s)	446, 1043
MaeII	ACGT	2 cut(s)	104, 1086
MaeIII	GTNAC	6 cut(s)	113, 163, 182, 458, 710, 1129
MalI	GATC	7 cut(s)	137, 358, 500, 562, 939, 1009, 1041
MboI	GATC	7 cut(s)	135, 356, 498, 560, 937, 1007, 1039
MhlI	GDGCHC	1 cut(s)	914
MluCI	AATT	4 cut(s)	305, 430, 578, 866
MlyI	GAGTC	1 cut(s)	820
MmeI	TCCRAC	1 cut(s)	63
MnlI	CCTC	6 cut(s)	6, 307, 320, 365, 568, 858
MroI	TCCGGA	1 cut(s)	945
MroXI	GAANNNNTTC	1 cut(s)	1101
MseI	TTAA	5 cut(s)	405, 723, 746, 804, 1165
MslI	CAYNNNNRTG	5 cut(s)	494, 534, 818, 957, 1145
MspA1I	CMGCKG	1 cut(s)	269
MspI	CCGG	3 cut(s)	718, 946, 1003
MvnI	CGCG	2 cut(s)	128, 1073
MwoI	GCNNNNNNNGC	6 cut(s)	13, 140, 350, 426, 442, 992
NdeI	CATATG	2 cut(s)	601, 1142
NdeII	GATC	7 cut(s)	135, 356, 498, 560, 937, 1007, 1039
NlaIII	CATG	3 cut(s)	817, 907, 956
NmuCI	GTSAC	4 cut(s)	113, 163, 458, 710
NsbI	TGCGCA	1 cut(s)	987
PaqCI	CACCTGC	1 cut(s)	641
PceI	AGGCCT	1 cut(s)	410
PciSI	GCTCTTC	2 cut(s)	312, 902
PdmI	GAANNNNTTC	1 cut(s)	1101
PfeI	GAWTC	7 cut(s)	172, 201, 473, 539, 795, 977, 1063
PkrI	GCNGC	7 cut(s)	18, 268, 385, 619, 966, 1077, 1111
Ple19I	CGATCG	1 cut(s)	138
PleI	GAGTC	1 cut(s)	820
PpsI	GAGTC	1 cut(s)	820
Ppu21I	YACGTR	1 cut(s)	105
Psp124BI	GAGCTC	1 cut(s)	914
PspFI	CCCAGC	1 cut(s)	287
PspPI	GGNCC	2 cut(s)	774, 1000
PstNI	CAGNNNCTG	1 cut(s)	491
PvuI	CGATCG	1 cut(s)	138
RsaI	GTAC	1 cut(s)	846
RsaNI	GTAC	1 cut(s)	845
RseI	CAYNNNNRTG	5 cut(s)	494, 534, 818, 957, 1145
SacI	GAGCTC	1 cut(s)	914
SapI	GCTCTTC	2 cut(s)	312, 902
SaqAI	TTAA	5 cut(s)	405, 723, 746, 804, 1165
SatI	GCNGC	7 cut(s)	17, 267, 384, 618, 965, 1076, 1110
Sau3AI	GATC	7 cut(s)	135, 356, 498, 560, 937, 1007, 1039
Sau96I	GGNCC	2 cut(s)	774, 1000
SchI	GAGTC	1 cut(s)	820
SduI	GDGCHC	1 cut(s)	914
SfaNI	GCATC	3 cut(s)	28, 232, 1035
SinI	GGWCC	2 cut(s)	774, 1000
SmiMI	CAYNNNNRTG	5 cut(s)	494, 534, 818, 957, 1145
Sse9I	AATT	4 cut(s)	305, 430, 578, 866
SseBI	AGGCCT	1 cut(s)	410
SsiI	CCGC	8 cut(s)	17, 49, 69, 81, 126, 269, 427, 1071
SspMI	CTAG	2 cut(s)	446, 1043
SstI	GAGCTC	1 cut(s)	914
StuI	AGGCCT	1 cut(s)	410
TaaI	ACNGT	5 cut(s)	119, 458, 493, 596, 1135
TaiI	ACGT	2 cut(s)	107, 1089
TaqI	TCGA	6 cut(s)	138, 249, 276, 559, 573, 798
TasI	AATT	4 cut(s)	305, 430, 578, 866
TauI	GCSGC	1 cut(s)	19
TfiI	GAWTC	7 cut(s)	172, 201, 473, 539, 795, 977, 1063
Tru1I	TTAA	5 cut(s)	405, 723, 746, 804, 1165
Tru9I	TTAA	5 cut(s)	405, 723, 746, 804, 1165
TscAI	CASTG	1 cut(s)	496
TseFI	GTSAC	4 cut(s)	113, 163, 458, 710
TseI	GCWGC	6 cut(s)	266, 383, 617, 964, 1075, 1109
Tsp45I	GTSAC	4 cut(s)	113, 163, 458, 710
TspDTI	ATGAA	6 cut(s)	14, 486, 511, 777, 859, 920
TspGWI	ACGGA	2 cut(s)	135, 224
TspRI	CASTG	1 cut(s)	496
VpaK11BI	GGWCC	2 cut(s)	774, 1000
XapI	RAATTY	2 cut(s)	305, 866
XmnI	GAANNNNTTC	1 cut(s)	1101
XspI	CTAG	2 cut(s)	446, 1043

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.