RchiOBHm_Chr5g0046991

Protein phosphatase 2C 57-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002125
Physical Location & Seq
Reverse (-)
43225549 .. 43229032
3484 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32494

Sequence Viewer

Length: 372 bp
ATGGCCACTGGAGCAATCAATGTGGTACGAGGTTCAAGGTCATCAGTTGAAGAATTGATACAAATATACACTCTGAAAGGTTCACCTTACCAACCTGGTTATACTGCCCCATCACCTCCCCCTCAGTCTGACGACACTACTGTTGATATCAGTGTTCAATTCAGTGGAGACCTTGTTACTGCATCTCCAGACGTTTATCAAGCAAGTCTAGGGGCAGACTCAGAATTTCTAGTGTTAGCATCTGATGGTTTATGGGATTACATAAACAGTTCAGATGCAGTTTCTTTTGTAAGGAATCAGCTTCGACAACATGGAGATGTCCAGCTAGCTTGTTATGCGCTTGCTGAAGCTGCTCTGGCAACAACTAATTGA

Protein Analysis

123

Amino Acids

13.05

Weight (kDa)

4.06

Isoelectric Point (pI)

45.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 57 - 121 1.1e-15 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 224
AcuI CTGAAG 1 cut(s) 366
AfaI GTAC 1 cut(s) 27
AgsI TTSAA 3 cut(s) 36, 50, 158
AjnI CCWGG 1 cut(s) 94
AluBI AGCT 4 cut(s) 301, 325, 329, 350
AluI AGCT 4 cut(s) 301, 325, 329, 350
Alw26I GTCTC 1 cut(s) 162
AoxI GGCC 1 cut(s) 3
ApeKI GCWGC 1 cut(s) 350
ApoI RAATTY 1 cut(s) 224
AspLEI GCGC 1 cut(s) 340
AsuHPI GGTGA 2 cut(s) 75, 105
AsuNHI GCTAGC 1 cut(s) 325
BalI TGGCCA 1 cut(s) 5
BarI GAAGNNNNNNTAC 2 cut(s) 42, 74
BbvI GCAGC 1 cut(s) 337
BccI CCATC 2 cut(s) 118, 239
BciT130I CCWGG 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 162
BfaI CTAG 3 cut(s) 209, 230, 326
BisI GCNGC 1 cut(s) 351
BlsI GCNGC 1 cut(s) 352
Bme1390I CCNGG 1 cut(s) 96
BmrFI CCNGG 1 cut(s) 96
BmsI GCATC 3 cut(s) 191, 248, 265
BmtI GCTAGC 1 cut(s) 329
BpmI CTGGAG 2 cut(s) 30, 171
BsaI GGTCTC 1 cut(s) 162
BsaXI ACNNNNNCTCC 4 cut(s) 159, 169, 189, 199
Bse1I ACTGG 1 cut(s) 13
BseBI CCWGG 1 cut(s) 96
BseMII CTCAG 2 cut(s) 137, 234
BseNI ACTGG 1 cut(s) 13
BseXI GCAGC 1 cut(s) 337
BshFI GGCC 1 cut(s) 5
BsmAI GTCTC 1 cut(s) 162
BsnI GGCC 1 cut(s) 5
Bso31I GGTCTC 1 cut(s) 162
BspANI GGCC 1 cut(s) 5
BspCNI CTCAG 2 cut(s) 136, 233
BspOI GCTAGC 1 cut(s) 329
BspTNI GGTCTC 1 cut(s) 162
BsrI ACTGG 1 cut(s) 13
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 2 cut(s) 142, 269
BstC8I GCNNGC 2 cut(s) 327, 342
BstDEI CTNAG 2 cut(s) 123, 220
BstHHI GCGC 1 cut(s) 340
BstMAI GTCTC 1 cut(s) 162
BstMWI GCNNNNNNNGC 4 cut(s) 11, 335, 350, 356
BstNI CCWGG 1 cut(s) 96
BstSCI CCNGG 1 cut(s) 94
BstV1I GCAGC 1 cut(s) 337
BsuRI GGCC 1 cut(s) 5
BtsIMutI CAGTG 3 cut(s) 6, 157, 169
Cac8I GCNNGC 2 cut(s) 327, 342
CfoI GCGC 1 cut(s) 340
CsiI ACCWGGT 1 cut(s) 94
Csp6I GTAC 1 cut(s) 26
CspCI CAANNNNNGTGG 1 cut(s) 38
CviAII CATG 1 cut(s) 311
CviJI RGCY 5 cut(s) 5, 301, 325, 329, 350
CviKI_1 RGCY 5 cut(s) 5, 301, 325, 329, 350
CviQI GTAC 1 cut(s) 26
DdeI CTNAG 2 cut(s) 123, 220
EaeI YGGCCR 1 cut(s) 3
Eco31I GGTCTC 1 cut(s) 162
Eco32I GATATC 1 cut(s) 148
Eco57I CTGAAG 1 cut(s) 366
EcoRII CCWGG 1 cut(s) 94
EcoRV GATATC 1 cut(s) 148
FaeI CATG 1 cut(s) 314
FaiI YATR 6 cut(s) 67, 102, 253, 263, 312, 336
FatI CATG 1 cut(s) 310
Fnu4HI GCNGC 1 cut(s) 351
Fsp4HI GCNGC 1 cut(s) 351
FspBI CTAG 3 cut(s) 209, 230, 326
GlaI GCGC 1 cut(s) 339
GluI GCNGC 1 cut(s) 351
GsuI CTGGAG 2 cut(s) 30, 171
HaeIII GGCC 1 cut(s) 5
HhaI GCGC 1 cut(s) 340
Hin1II CATG 1 cut(s) 314
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HinfI GANTC 2 cut(s) 218, 295
HphI GGTGA 2 cut(s) 75, 105
Hpy166II GTNNAC 1 cut(s) 83
Hpy188I TCNGA 5 cut(s) 75, 130, 223, 244, 274
Hpy188III TCNNGA 1 cut(s) 188
Hpy8I GTNNAC 1 cut(s) 83
HpyCH4III ACNGT 2 cut(s) 142, 269
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 2 cut(s) 182, 278
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 335, 350, 356
HpyF3I CTNAG 2 cut(s) 123, 220
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 1 cut(s) 314
HspAI GCGC 1 cut(s) 338
LmnI GCTCC 1 cut(s) 11
LpnPI CCDG 5 cut(s) 81, 108, 201, 335, 341
Lsp1109I GCAGC 1 cut(s) 337
LweI GCATC 3 cut(s) 191, 248, 265
MabI ACCWGGT 1 cut(s) 94
MaeI CTAG 3 cut(s) 209, 230, 326
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 175
MboII GAAGA 1 cut(s) 62
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 53, 158, 224, 367
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 212
MnlI CCTC 3 cut(s) 23, 126, 132
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 315
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 96
MvaI CCWGG 1 cut(s) 96
MwoI GCNNNNNNNGC 4 cut(s) 11, 335, 350, 356
NheI GCTAGC 1 cut(s) 325
NlaIII CATG 1 cut(s) 314
PfeI GAWTC 1 cut(s) 295
PkrI GCNGC 1 cut(s) 352
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp6I CCWGG 1 cut(s) 94
PspGI CCWGG 1 cut(s) 94
RsaI GTAC 1 cut(s) 27
RsaNI GTAC 1 cut(s) 26
RseI CAYNNNNRTG 1 cut(s) 315
SatI GCNGC 1 cut(s) 351
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 1 cut(s) 96
SexAI ACCWGGT 1 cut(s) 94
SfaNI GCATC 3 cut(s) 191, 248, 265
SmiMI CAYNNNNRTG 1 cut(s) 315
Sse9I AATT 4 cut(s) 53, 158, 224, 367
SspMI CTAG 3 cut(s) 209, 230, 326
StyD4I CCNGG 1 cut(s) 94
TaaI ACNGT 2 cut(s) 142, 269
TaiI ACGT 1 cut(s) 195
TaqI TCGA 1 cut(s) 304
TasI AATT 4 cut(s) 53, 158, 224, 367
TfiI GAWTC 1 cut(s) 295
TscAI CASTG 3 cut(s) 13, 157, 169
TseI GCWGC 1 cut(s) 350
TspRI CASTG 3 cut(s) 13, 157, 169
XapI RAATTY 1 cut(s) 224
XspI CTAG 3 cut(s) 209, 230, 326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.