AT4G28510

SPFH domain / Band 7 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001997
Physical Location & Seq
Reverse (-)
14084624 .. 14086882
2259 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G28510.1

Sequence Viewer

Length: 867 bp
ATGAACAACGTCAAAGTTCCAAAGATACCAGGTGGTGGTGCCATTTCGACGTTGCTTAAGGTTGGGATTATTGGTGGGCTTGGTCTCTATGGTGCTACGCACAGTCTCTACAATGTTGAAGGAGGACATCGAGCCATCATGTTCAATCGTTTAGTCGGTATTAAAGATAAGGTTTACCCTGAGGGTACACACCTTATGATTCCTTGGTTTGAAAGGCCGGTCATCTATGACGTTCGTGCTCGACCTTACCTTGTTGAGAGTACATCCGGAAGCCGTGATCTTCAGATGGTGAAAATTGGGCTTAGGGTTCTCACACGTCCCATGGCAGACCAGTTACCTGAAATCTACAGAAGCCTTGGTGAGAACTACAGCGAGAGAGTTCTACCTTCTATAATCAACGAGACTTTGAAAGCTGTGGTTGCTCAGTACAATGCAAGCCAGCTTATTACTCAGAGAGAGGCGGTCAGTAGGGAGATCAGGAAGATTCTGACTGAACGAGCAGCAAACTTCAATGTTGCGCTTGACGATGTGTCCATCACAAACCTGACATTCGGGAAGGAGTTCACAGCTGCCATTGAAGCAAAGCAGGTGGCGGCTCAAGAGGCTGAGCGGGCTAAGTTCATTGTTGAGAAGGCCGAACAAGACAAGAGAAGTGCTGTTATCCGCGCCCAGGGAGAAGCCAAGAGTGCTCAGCTCATTGGTCAAGCAATTGCAAACAACCAAGCGTTTATCACGCTCAGGAAGATCGAGGCTGCAAGAGAGATTGCACAGACCATAGCAAACTCGGCGAACAAGGTTTACTTGAGCTCAGACGATCTTTTGCTTAACCTACAAGGGATGAATTTGGATGTTGATGCAAAGAACTAG

Protein Analysis

288

Amino Acids

31.71

Weight (kDa)

9.26

Isoelectric Point (pI)

30.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 38 - 213 9.2e-33 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 577
Acc36I ACCTGC 1 cut(s) 577
AccB1I GGYRCC 1 cut(s) 38
AccB7I CCANNNNNTGG 1 cut(s) 35
AccBSI CCGCTC 1 cut(s) 610
AccII CGCG 1 cut(s) 666
AccIII TCCGGA 1 cut(s) 266
AciI CCGC 4 cut(s) 461, 593, 610, 664
AcsI RAATTY 1 cut(s) 841
AcuI CTGAAG 1 cut(s) 266
AfaI GTAC 3 cut(s) 187, 262, 428
AfiI CCNNNNNNNGG 2 cut(s) 35, 670
AflII CTTAAG 1 cut(s) 56
AflIII ACRYGT 1 cut(s) 314
AgsI TTSAA 6 cut(s) 119, 145, 212, 409, 511, 578
AhdI GACNNNNNGTC 1 cut(s) 529
AjiI CACGTC 1 cut(s) 317
AjnI CCWGG 2 cut(s) 28, 669
AluBI AGCT 5 cut(s) 413, 442, 569, 694, 807
AluI AGCT 5 cut(s) 413, 442, 569, 694, 807
Alw21I GWGCWC 3 cut(s) 241, 691, 809
Alw26I GTCTC 3 cut(s) 89, 110, 395
Aor13HI TCCGGA 1 cut(s) 266
AoxI GGCC 2 cut(s) 215, 633
ApeKI GCWGC 3 cut(s) 500, 569, 752
ApoI RAATTY 1 cut(s) 841
Asp700I GAANNNNTTC 1 cut(s) 560
AspLEI GCGC 2 cut(s) 520, 668
AsuHPI GGTGA 2 cut(s) 301, 371
AxyI CCTNAGG 1 cut(s) 180
BanI GGYRCC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 809
Bbv12I GWGCWC 3 cut(s) 241, 691, 809
BbvI GCAGC 3 cut(s) 512, 556, 739
BccI CCATC 3 cut(s) 143, 280, 542
BceAI ACGGC 1 cut(s) 258
BciT130I CCWGG 2 cut(s) 30, 671
BcoDI GTCTC 3 cut(s) 89, 110, 395
BfaI CTAG 1 cut(s) 865
BfmI CTRYAG 2 cut(s) 346, 367
BfrI CTTAAG 1 cut(s) 56
BfuAI ACCTGC 1 cut(s) 577
BisI GCNGC 4 cut(s) 501, 570, 594, 753
BlpI GCTNAGC 2 cut(s) 606, 690
BlsI GCNGC 4 cut(s) 502, 571, 595, 754
Bme1390I CCNGG 2 cut(s) 30, 671
BmeRI GACNNNNNGTC 1 cut(s) 529
BmgBI CACGTC 1 cut(s) 317
BmiI GGNNCC 1 cut(s) 40
BmrFI CCNGG 2 cut(s) 30, 671
BmsI GCATC 1 cut(s) 844
Bpu10I CCTNAGC 2 cut(s) 302, 737
Bpu1102I GCTNAGC 2 cut(s) 606, 690
BpuEI CTTGAG 2 cut(s) 582, 823
BsaI GGTCTC 1 cut(s) 89
BsaJI CCNNGG 5 cut(s) 203, 321, 355, 669, 670
BsaWI WCCGGW 1 cut(s) 266
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 670
Bse118I RCCGGY 1 cut(s) 217
Bse1I ACTGG 1 cut(s) 331
Bse21I CCTNAGG 1 cut(s) 180
BseAI TCCGGA 1 cut(s) 266
BseBI CCWGG 2 cut(s) 30, 671
BseDI CCNNGG 5 cut(s) 203, 321, 355, 669, 670
BseGI GGATG 3 cut(s) 263, 843, 853
BseLI CCNNNNNNNGG 2 cut(s) 35, 670
BseMII CTCAG 7 cut(s) 171, 437, 464, 597, 704, 751, 822
BseNI ACTGG 1 cut(s) 331
BseXI GCAGC 3 cut(s) 512, 556, 739
Bsh1236I CGCG 1 cut(s) 666
BshFI GGCC 2 cut(s) 217, 635
BshNI GGYRCC 1 cut(s) 38
BsiHKAI GWGCWC 3 cut(s) 241, 691, 809
BsiSI CCGG 2 cut(s) 218, 267
BslFI GGGAC 1 cut(s) 303
BslI CCNNNNNNNGG 2 cut(s) 35, 670
BsmAI GTCTC 3 cut(s) 89, 110, 395
BsmFI GGGAC 1 cut(s) 303
BsnI GGCC 2 cut(s) 217, 635
Bso31I GGTCTC 1 cut(s) 89
Bsp1286I GDGCHC 3 cut(s) 241, 691, 809
Bsp13I TCCGGA 1 cut(s) 266
Bsp143I GATC 4 cut(s) 277, 474, 744, 814
Bsp1720I GCTNAGC 2 cut(s) 606, 690
Bsp19I CCATGG 1 cut(s) 321
BspACI CCGC 4 cut(s) 461, 593, 610, 664
BspANI GGCC 2 cut(s) 217, 635
BspCNI CTCAG 7 cut(s) 172, 436, 463, 598, 703, 750, 821
BspEI TCCGGA 1 cut(s) 266
BspFNI CGCG 1 cut(s) 666
BspLI GGNNCC 1 cut(s) 40
BspMI ACCTGC 1 cut(s) 577
BspT107I GGYRCC 1 cut(s) 38
BspTI CTTAAG 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 610
BsrFI RCCGGY 1 cut(s) 217
BsrI ACTGG 1 cut(s) 331
BssAI RCCGGY 1 cut(s) 217
BssECI CCNNGG 5 cut(s) 203, 321, 355, 669, 670
BssMI GATC 4 cut(s) 277, 474, 744, 814
BssT1I CCWWGG 3 cut(s) 203, 321, 355
Bst2UI CCWGG 2 cut(s) 30, 671
Bst4CI ACNGT 1 cut(s) 104
BstAFI CTTAAG 1 cut(s) 56
BstC8I GCNNGC 3 cut(s) 436, 440, 612
BstDEI CTNAG 9 cut(s) 180, 302, 423, 450, 606, 615, 690, 737, 808
BstDSI CCRYGG 1 cut(s) 321
BstF5I GGATG 3 cut(s) 263, 843, 853
BstFNI CGCG 1 cut(s) 666
BstHHI GCGC 2 cut(s) 520, 668
BstKTI GATC 4 cut(s) 280, 477, 747, 817
BstMAI GTCTC 3 cut(s) 89, 110, 395
BstMBI GATC 4 cut(s) 277, 474, 744, 814
BstMWI GCNNNNNNNGC 6 cut(s) 419, 578, 602, 611, 686, 785
BstNI CCWGG 2 cut(s) 30, 671
BstSCI CCNGG 2 cut(s) 28, 669
BstSFI CTRYAG 2 cut(s) 346, 367
BstUI CGCG 1 cut(s) 666
BstV1I GCAGC 3 cut(s) 512, 556, 739
Bsu36I CCTNAGG 1 cut(s) 180
BsuRI GGCC 2 cut(s) 217, 635
BtgI CCRYGG 1 cut(s) 321
BtrI CACGTC 1 cut(s) 317
BtsCI GGATG 3 cut(s) 263, 843, 853
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 3 cut(s) 436, 440, 612
CfoI GCGC 2 cut(s) 520, 668
Cfr10I RCCGGY 1 cut(s) 217
CsiI ACCWGGT 1 cut(s) 28
Csp6I GTAC 3 cut(s) 186, 261, 427
CspCI CAANNNNNGTGG 2 cut(s) 570, 605
CviAII CATG 2 cut(s) 139, 322
CviQI GTAC 3 cut(s) 186, 261, 427
DdeI CTNAG 9 cut(s) 180, 302, 423, 450, 606, 615, 690, 737, 808
DpnI GATC 4 cut(s) 279, 476, 746, 816
DpnII GATC 4 cut(s) 277, 474, 744, 814
DriI GACNNNNNGTC 1 cut(s) 529
Eam1105I GACNNNNNGTC 1 cut(s) 529
Ecl136II GAGCTC 1 cut(s) 807
Eco130I CCWWGG 3 cut(s) 203, 321, 355
Eco24I GRGCYC 1 cut(s) 809
Eco31I GGTCTC 1 cut(s) 89
Eco53kI GAGCTC 1 cut(s) 807
Eco57I CTGAAG 1 cut(s) 266
Eco81I CCTNAGG 1 cut(s) 180
EcoICRI GAGCTC 1 cut(s) 807
EcoRII CCWGG 2 cut(s) 28, 669
EcoT14I CCWWGG 3 cut(s) 203, 321, 355
EcoT38I GRGCYC 1 cut(s) 809
ErhI CCWWGG 3 cut(s) 203, 321, 355
FaeI CATG 2 cut(s) 142, 325
FaiI YATR 7 cut(s) 90, 140, 197, 228, 323, 392, 776
FalI AAGNNNNNCTT 2 cut(s) 785, 817
FaqI GGGAC 1 cut(s) 303
FatI CATG 2 cut(s) 138, 321
FauI CCCGC 1 cut(s) 603
Fnu4HI GCNGC 4 cut(s) 501, 570, 594, 753
FokI GGATG 3 cut(s) 250, 850, 860
FriOI GRGCYC 1 cut(s) 809
Fsp4HI GCNGC 4 cut(s) 501, 570, 594, 753
FspBI CTAG 1 cut(s) 865
GlaI GCGC 2 cut(s) 519, 667
GluI GCNGC 4 cut(s) 501, 570, 594, 753
HaeIII GGCC 2 cut(s) 217, 635
HapII CCGG 2 cut(s) 218, 267
HhaI GCGC 2 cut(s) 520, 668
Hin1II CATG 2 cut(s) 142, 325
Hin6I GCGC 2 cut(s) 518, 666
HinP1I GCGC 2 cut(s) 518, 666
HinfI GANTC 2 cut(s) 199, 484
HpaII CCGG 2 cut(s) 218, 267
HphI GGTGA 2 cut(s) 301, 371
Hpy166II GTNNAC 4 cut(s) 175, 188, 564, 799
Hpy188I TCNGA 4 cut(s) 285, 453, 489, 811
Hpy188III TCNNGA 5 cut(s) 267, 478, 553, 599, 739
Hpy8I GTNNAC 4 cut(s) 175, 188, 564, 799
Hpy99I CGWCG 1 cut(s) 52
HpyAV CCTTC 4 cut(s) 113, 396, 550, 625
HpyCH4III ACNGT 1 cut(s) 104
HpyCH4IV ACGT 4 cut(s) 9, 50, 231, 316
HpyCH4V TGCA 5 cut(s) 434, 713, 755, 767, 857
HpyF10VI GCNNNNNNNGC 6 cut(s) 419, 578, 602, 611, 686, 785
HpyF3I CTNAG 9 cut(s) 180, 302, 423, 450, 606, 615, 690, 737, 808
HpySE526I ACGT 4 cut(s) 9, 50, 231, 316
Hsp92II CATG 2 cut(s) 142, 325
HspAI GCGC 2 cut(s) 518, 666
Kpn2I TCCGGA 1 cut(s) 266
Kzo9I GATC 4 cut(s) 277, 474, 744, 814
Lsp1109I GCAGC 3 cut(s) 512, 556, 739
LweI GCATC 1 cut(s) 844
MabI ACCWGGT 1 cut(s) 28
MaeI CTAG 1 cut(s) 865
MaeII ACGT 4 cut(s) 9, 50, 231, 316
MaeIII GTNAC 1 cut(s) 333
MalI GATC 4 cut(s) 279, 476, 746, 816
MbiI CCGCTC 1 cut(s) 610
MboI GATC 4 cut(s) 277, 474, 744, 814
MboII GAAGA 3 cut(s) 272, 493, 754
MfeI CAATTG 1 cut(s) 708
MhlI GDGCHC 3 cut(s) 241, 691, 809
MluCI AATT 3 cut(s) 294, 708, 841
MnlI CCTC 5 cut(s) 116, 175, 451, 595, 742
MroI TCCGGA 1 cut(s) 266
MroXI GAANNNNTTC 1 cut(s) 560
MseI TTAA 3 cut(s) 57, 162, 825
MspA1I CMGCKG 1 cut(s) 569
MspCI CTTAAG 1 cut(s) 56
MspI CCGG 2 cut(s) 218, 267
MspR9I CCNGG 2 cut(s) 30, 671
MunI CAATTG 1 cut(s) 708
MvaI CCWGG 2 cut(s) 30, 671
MvnI CGCG 1 cut(s) 666
MwoI GCNNNNNNNGC 6 cut(s) 419, 578, 602, 611, 686, 785
NcoI CCATGG 1 cut(s) 321
NdeII GATC 4 cut(s) 277, 474, 744, 814
NlaIII CATG 2 cut(s) 142, 325
NlaIV GGNNCC 1 cut(s) 40
NmeAIII GCCGAG 1 cut(s) 764
PaqCI CACCTGC 1 cut(s) 577
PasI CCCWGGG 1 cut(s) 670
PdmI GAANNNNTTC 1 cut(s) 560
PfeI GAWTC 2 cut(s) 199, 484
PflMI CCANNNNNTGG 1 cut(s) 35
PkrI GCNGC 4 cut(s) 502, 571, 595, 754
Psp124BI GAGCTC 1 cut(s) 809
Psp6I CCWGG 2 cut(s) 28, 669
PspGI CCWGG 2 cut(s) 28, 669
PspN4I GGNNCC 1 cut(s) 40
PsrI GAACNNNNNNTAC 2 cut(s) 782, 814
PvuII CAGCTG 1 cut(s) 569
RsaI GTAC 3 cut(s) 187, 262, 428
RsaNI GTAC 3 cut(s) 186, 261, 427
SacI GAGCTC 1 cut(s) 809
SaqAI TTAA 3 cut(s) 57, 162, 825
SatI GCNGC 4 cut(s) 501, 570, 594, 753
Sau3AI GATC 4 cut(s) 277, 474, 744, 814
ScrFI CCNGG 2 cut(s) 30, 671
SduI GDGCHC 3 cut(s) 241, 691, 809
SexAI ACCWGGT 1 cut(s) 28
SfaNI GCATC 1 cut(s) 844
SfcI CTRYAG 2 cut(s) 346, 367
SmlI CTYRAG 3 cut(s) 56, 597, 802
SmoI CTYRAG 3 cut(s) 56, 597, 802
Sse9I AATT 3 cut(s) 294, 708, 841
SsiI CCGC 4 cut(s) 461, 593, 610, 664
SspMI CTAG 1 cut(s) 865
SstI GAGCTC 1 cut(s) 809
StyD4I CCNGG 2 cut(s) 28, 669
StyI CCWWGG 3 cut(s) 203, 321, 355
TaaI ACNGT 1 cut(s) 104
TaiI ACGT 4 cut(s) 12, 53, 234, 319
TaqI TCGA 4 cut(s) 47, 130, 241, 747
TasI AATT 3 cut(s) 294, 708, 841
TatI WGTACW 2 cut(s) 260, 426
TauI GCSGC 1 cut(s) 596
TfiI GAWTC 2 cut(s) 199, 484
Tru1I TTAA 3 cut(s) 57, 162, 825
Tru9I TTAA 3 cut(s) 57, 162, 825
TseI GCWGC 3 cut(s) 500, 569, 752
TspDTI ATGAA 3 cut(s) 17, 610, 854
Van91I CCANNNNNTGG 1 cut(s) 35
Vha464I CTTAAG 1 cut(s) 56
XapI RAATTY 1 cut(s) 841
XmnI GAANNNNTTC 1 cut(s) 560
XspI CTAG 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.