Rorug05G0189600

Prohibitin-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001997
Physical Location & Seq
Forward (+)
18421645 .. 18422183
539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0189600.1

Sequence Viewer

Length: 408 bp
ATGCAAGCATCAAGAGCAAACTCGGATTGTAGAAGAGTTTCATCAGCATGGTGTCCAATTGTGAACATGAGGAATATATTGGCGCAGCCCTCTACAGAAAACTTGATGGGCAATCTTTTAGGGTTCTTTGTAGCAATATCAACAGGAGCTGAAAGTGATGATGATCTTCAAGGCCTGGTTGCTAAAATGAGGAAGCGGTTTCCTTTCTATGAAGCCAATTTTGGATGGGGAAAGCCACTGATGAGTATTCCTGCTACTGAAGAACTCAAGAATTTGATTACGTTGATGGATATTAGTTATGGAGATGGTATAGAAGTGCGCTTGACTTTGAAAGAAGAAGACATGGCCATATTTGAAAACAATGAGGAGCTGCTAGCATATGCTTCTTTAAATCCCTCTGTCATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Band_7

Protein Analysis

135

Amino Acids

15.04

Weight (kDa)

4.62

Isoelectric Point (pI)

54.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 60 - 124 9.9e-11 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 196
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 1 cut(s) 271
AcuI CTGAAG 1 cut(s) 279
AgsI TTSAA 3 cut(s) 170, 331, 356
AjnI CCWGG 1 cut(s) 174
AjuI GAANNNNNNNTTGG 2 cut(s) 204, 236
AluBI AGCT 2 cut(s) 149, 370
AluI AGCT 2 cut(s) 149, 370
AlwNI CAGNNNCTG 1 cut(s) 149
AoxI GGCC 2 cut(s) 172, 345
ApeKI GCWGC 2 cut(s) 85, 370
ApoI RAATTY 1 cut(s) 271
Asp700I GAANNNNTTC 1 cut(s) 37
AspLEI GCGC 2 cut(s) 85, 321
AsuNHI GCTAGC 1 cut(s) 373
BalI TGGCCA 1 cut(s) 347
BbsI GAAGAC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 97, 357
BccI CCATC 4 cut(s) 100, 219, 280, 299
BciT130I CCWGG 1 cut(s) 176
BfaI CTAG 1 cut(s) 374
BfmI CTRYAG 1 cut(s) 93
BisI GCNGC 2 cut(s) 86, 371
BlsI GCNGC 2 cut(s) 87, 372
Bme1390I CCNGG 1 cut(s) 176
BmrFI CCNGG 1 cut(s) 176
BmsI GCATC 1 cut(s) 17
BmtI GCTAGC 1 cut(s) 377
BpiI GAAGAC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 251
BsaBI GATNNNNATC 1 cut(s) 162
Bse8I GATNNNNATC 1 cut(s) 162
BseBI CCWGG 1 cut(s) 176
BseGI GGATG 1 cut(s) 230
BseJI GATNNNNATC 1 cut(s) 162
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 2 cut(s) 97, 357
BshFI GGCC 2 cut(s) 174, 347
BsnI GGCC 2 cut(s) 174, 347
Bsp143I GATC 1 cut(s) 163
BspACI CCGC 1 cut(s) 196
BspANI GGCC 2 cut(s) 174, 347
BspOI GCTAGC 1 cut(s) 377
BssMI GATC 1 cut(s) 163
Bst2UI CCWGG 1 cut(s) 176
Bst6I CTCTTC 1 cut(s) 28
BstC8I GCNNGC 2 cut(s) 6, 375
BstF5I GGATG 1 cut(s) 230
BstHHI GCGC 2 cut(s) 85, 321
BstKTI GATC 1 cut(s) 166
BstMBI GATC 1 cut(s) 163
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNI CCWGG 1 cut(s) 176
BstSCI CCNGG 1 cut(s) 174
BstSFI CTRYAG 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 97, 357
BstV2I GAAGAC 1 cut(s) 345
BsuRI GGCC 2 cut(s) 174, 347
BtsCI GGATG 1 cut(s) 230
BtsIMutI CAGTG 1 cut(s) 236
Cac8I GCNNGC 2 cut(s) 6, 375
CaiI CAGNNNCTG 1 cut(s) 149
CfoI GCGC 2 cut(s) 85, 321
CviAII CATG 3 cut(s) 48, 67, 343
CviJI RGCY 7 cut(s) 88, 149, 174, 215, 235, 347, 370
CviKI_1 RGCY 7 cut(s) 88, 149, 174, 215, 235, 347, 370
DpnI GATC 1 cut(s) 165
DpnII GATC 1 cut(s) 163
DraI TTTAAA 1 cut(s) 390
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 1 cut(s) 28
EarI CTCTTC 1 cut(s) 28
Eco147I AGGCCT 1 cut(s) 174
Eco57I CTGAAG 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 174
FaeI CATG 3 cut(s) 51, 70, 346
FatI CATG 3 cut(s) 47, 66, 342
FauNDI CATATG 1 cut(s) 379
Fnu4HI GCNGC 2 cut(s) 86, 371
FokI GGATG 1 cut(s) 237
Fsp4HI GCNGC 2 cut(s) 86, 371
FspBI CTAG 1 cut(s) 374
GlaI GCGC 2 cut(s) 84, 320
GluI GCNGC 2 cut(s) 86, 371
HaeIII GGCC 2 cut(s) 174, 347
HhaI GCGC 2 cut(s) 85, 321
Hin1II CATG 3 cut(s) 51, 70, 346
Hin6I GCGC 2 cut(s) 83, 319
HinP1I GCGC 2 cut(s) 83, 319
Hpy166II GTNNAC 1 cut(s) 64
Hpy188I TCNGA 1 cut(s) 25
Hpy188III TCNNGA 2 cut(s) 12, 268
Hpy8I GTNNAC 1 cut(s) 64
HpyCH4IV ACGT 1 cut(s) 281
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpySE526I ACGT 1 cut(s) 281
Hsp92II CATG 3 cut(s) 51, 70, 346
HspAI GCGC 2 cut(s) 83, 319
Kzo9I GATC 1 cut(s) 163
LmnI GCTCC 2 cut(s) 146, 367
LpnPI CCDG 4 cut(s) 129, 161, 188, 264
Lsp1109I GCAGC 2 cut(s) 97, 357
LweI GCATC 1 cut(s) 17
MaeI CTAG 1 cut(s) 374
MaeII ACGT 1 cut(s) 281
MalI GATC 1 cut(s) 165
MboI GATC 1 cut(s) 163
MboII GAAGA 5 cut(s) 45, 158, 272, 347, 350
MfeI CAATTG 1 cut(s) 57
MlsI TGGCCA 1 cut(s) 347
MluCI AATT 3 cut(s) 57, 217, 271
MluNI TGGCCA 1 cut(s) 347
MnlI CCTC 5 cut(s) 63, 100, 183, 358, 406
Mox20I TGGCCA 1 cut(s) 347
MroXI GAANNNNTTC 1 cut(s) 37
MscI TGGCCA 1 cut(s) 347
MseI TTAA 1 cut(s) 389
MslI CAYNNNNRTG 1 cut(s) 46
Msp20I TGGCCA 1 cut(s) 347
MspR9I CCNGG 1 cut(s) 176
MunI CAATTG 1 cut(s) 57
MvaI CCWGG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 14
NdeI CATATG 1 cut(s) 379
NdeII GATC 1 cut(s) 163
NheI GCTAGC 1 cut(s) 373
NlaIII CATG 3 cut(s) 51, 70, 346
PceI AGGCCT 1 cut(s) 174
PdmI GAANNNNTTC 1 cut(s) 37
PkrI GCNGC 2 cut(s) 87, 372
Psp6I CCWGG 1 cut(s) 174
PspGI CCWGG 1 cut(s) 174
PstNI CAGNNNCTG 1 cut(s) 149
RseI CAYNNNNRTG 1 cut(s) 46
SaqAI TTAA 1 cut(s) 389
SatI GCNGC 2 cut(s) 86, 371
Sau3AI GATC 1 cut(s) 163
ScrFI CCNGG 1 cut(s) 176
SetI ASST 3 cut(s) 151, 284, 372
SfaNI GCATC 1 cut(s) 17
SfcI CTRYAG 1 cut(s) 93
SmiMI CAYNNNNRTG 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
Sse9I AATT 3 cut(s) 57, 217, 271
SseBI AGGCCT 1 cut(s) 174
SsiI CCGC 1 cut(s) 196
SspMI CTAG 1 cut(s) 374
StuI AGGCCT 1 cut(s) 174
StyD4I CCNGG 1 cut(s) 174
TaiI ACGT 1 cut(s) 284
TasI AATT 3 cut(s) 57, 217, 271
Tru1I TTAA 1 cut(s) 389
Tru9I TTAA 1 cut(s) 389
TscAI CASTG 1 cut(s) 243
TseI GCWGC 2 cut(s) 85, 370
TspDTI ATGAA 2 cut(s) 30, 225
TspRI CASTG 1 cut(s) 243
XapI RAATTY 1 cut(s) 271
XmnI GAANNNNTTC 1 cut(s) 37
XspI CTAG 1 cut(s) 374
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.