MD05G1030500.v1.1

Prohibitin-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0001997
Physical Location & Seq
Forward (+)
4898213 .. 4901434
3222 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1030500.v1.1.491

Sequence Viewer

Length: 1140 bp
ATGAAAAGTGAGACTGATTCCTGTACCGAATTTTCAGGATCGGTTTTGGATTTTCAATTTGAAATTTTTTTGGTTTGTTGGACCTTGGCCGATACTCTAGGCCTTTTAAAGGCCGCCTTAAACCTTTCGCGTTCTCGCTCGTTACCTGTAAACCCTAAACCCTTGCATCTGCTGCTGGGGCCTGAATCCTCCGCATCATCCTCATCACCGTTTCTCCACCGTTGCGACAGCCACGTTCCTCACCTCTCACTGGTTCCGAGAGTGAATATGAATTTCAACAACGTTAAGGTTCCGAAAGTTCCCGGTGGTGGTGCAGTTTCTGCTTTGCTTAAGGTTGGCATAATTGGTGGGCTTGGCTTGTATGGAGCTGCCAACAGTCTGTACAATGTCGAGGGAGGGCATCGAGCCATTGTGTTCAACCGGATAATCGGTGTCAAGGACAAGGTCTATCCAGAGGGTACACACCTCATCATTCCATGGTTTGAGAGGCCAGTCATTTATGATGTCCGTGCACGACCTCATCTAGTGGAGAGTACTTCTGGGAGCCGTGATCTCCAAATGGTGAAAATTGGGCTTCGAGTGCTTACTCGACCTCTACCAGACCAGCTGCCTACAGTTTATCGGACTCTTGGTGAGAATTACAATGAGAGAGTCCTTCCTTCCATTGTTCATGAAACTTTGAAAGCTGTTGTTGCACAGTATAATGCTAGTCAGCTCATTACTCAGAGAGAGACTGTCAGTAGGGAAATACGAAAGATTTTGACTGAAAGGGCTGCCAATTTCAACATTGCGCTGGACGATGTGTCAATTACAACTCTTACTTTTGGGAAGGAGTTCACGGCTGCAATTGAGGCCAAACAAGTGGCTGCACAGGAAGCTGAGAGAGCCAAGTTTGTTGTGGACAAGGCTGAACAAGACAAGAAGAGTGCTATCATCAGAGCAGAGGGAGAGGCGACAAGTGCCAAGCTGATTGGTGAAGCTATTGCAAACAATCCGGCATTTATTACACTGAGGAAGATTGAGGCTGCAAGAGAGATTGCACATACAATCTCAAATTCCGCAAACAAAGTTTTCTTGAACTCAGATGATCTGTTGCTGAACCTTCAGGAGATGAATTTGGATGTCAACAAGAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Band_7

Protein Analysis

380

Amino Acids

41.64

Weight (kDa)

8.97

Isoelectric Point (pI)

36.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 129 - 306 2.1e-31 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 130
AciI CCGC 3 cut(s) 114, 192, 1059
AclI AACGTT 1 cut(s) 282
AclWI GGATC 1 cut(s) 46
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 5 cut(s) 29, 63, 271, 1054, 1114
AcuI CTGAAG 1 cut(s) 1088
AfaI GTAC 4 cut(s) 25, 383, 460, 535
AfiI CCNNNNNNNGG 3 cut(s) 109, 250, 308
AflII CTTAAG 1 cut(s) 329
AgsI TTSAA 7 cut(s) 56, 62, 277, 418, 682, 784, 1078
AhdI GACNNNNNGTC 1 cut(s) 802
AluBI AGCT 7 cut(s) 368, 607, 686, 715, 878, 967, 980
AluI AGCT 7 cut(s) 368, 607, 686, 715, 878, 967, 980
Alw21I GWGCWC 1 cut(s) 514
Alw26I GTCTC 2 cut(s) 5, 725
Alw44I GTGCAC 1 cut(s) 510
AlwI GGATC 1 cut(s) 46
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 6 cut(s) 87, 100, 111, 179, 488, 852
ApaLI GTGCAC 1 cut(s) 510
ApeKI GCWGC 7 cut(s) 172, 368, 607, 773, 842, 866, 1025
ApoI RAATTY 5 cut(s) 29, 63, 271, 1054, 1114
Asp700I GAANNNNTTC 1 cut(s) 833
AspLEI GCGC 1 cut(s) 793
AspS9I GGNCC 2 cut(s) 81, 179
AsuC2I CCSGG 1 cut(s) 303
AsuHPI GGTGA 5 cut(s) 198, 233, 574, 644, 986
AvaII GGWCC 1 cut(s) 81
BaeGI GKGCMC 1 cut(s) 514
Bbv12I GWGCWC 1 cut(s) 514
BbvI GCAGC 7 cut(s) 159, 355, 594, 760, 829, 853, 1012
BceAI ACGGC 2 cut(s) 531, 855
BcnI CCSGG 1 cut(s) 303
BcoDI GTCTC 2 cut(s) 5, 725
BfaI CTAG 3 cut(s) 98, 524, 708
BfmI CTRYAG 1 cut(s) 612
BfrI CTTAAG 1 cut(s) 329
BisI GCNGC 8 cut(s) 114, 173, 369, 608, 774, 843, 867, 1026
BlsI GCNGC 8 cut(s) 115, 174, 370, 609, 775, 844, 868, 1027
BmcAI AGTACT 1 cut(s) 535
Bme1390I CCNGG 1 cut(s) 303
Bme18I GGWCC 1 cut(s) 81
BmeRI GACNNNNNGTC 1 cut(s) 802
BmgT120I GGNCC 2 cut(s) 81, 179
BmiI GGNNCC 4 cut(s) 180, 255, 291, 545
BmrFI CCNGG 1 cut(s) 303
BmsI GCATC 3 cut(s) 175, 203, 409
BpuMI CCSGG 1 cut(s) 303
BsaJI CCNNGG 2 cut(s) 84, 476
BsaWI WCCGGW 1 cut(s) 420
BsaXI ACNNNNNCTCC 2 cut(s) 198, 228
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 250, 308
Bse1I ACTGG 2 cut(s) 255, 491
Bse3DI GCAATG 1 cut(s) 786
BseDI CCNNGG 2 cut(s) 84, 476
BseGI GGATG 2 cut(s) 197, 1126
BseLI CCNNNNNNNGG 3 cut(s) 109, 250, 308
BseMI GCAATG 1 cut(s) 786
BseMII CTCAG 4 cut(s) 737, 870, 1001, 1095
BseNI ACTGG 2 cut(s) 255, 491
BseSI GKGCMC 1 cut(s) 514
BseXI GCAGC 7 cut(s) 159, 355, 594, 760, 829, 853, 1012
BseYI CCCAGC 1 cut(s) 175
BsgI GTGCAG 2 cut(s) 333, 852
Bsh1236I CGCG 1 cut(s) 130
BshFI GGCC 6 cut(s) 89, 102, 113, 181, 490, 854
BsiHKAI GWGCWC 1 cut(s) 514
BsiSI CCGG 3 cut(s) 303, 421, 995
BslI CCNNNNNNNGG 3 cut(s) 109, 250, 308
BsmAI GTCTC 2 cut(s) 5, 725
BsnI GGCC 6 cut(s) 89, 102, 113, 181, 490, 854
Bsp1286I GDGCHC 1 cut(s) 514
Bsp1407I TGTACA 1 cut(s) 381
Bsp143I GATC 3 cut(s) 38, 550, 1087
Bsp19I CCATGG 1 cut(s) 476
BspACI CCGC 3 cut(s) 114, 192, 1059
BspANI GGCC 6 cut(s) 89, 102, 113, 181, 490, 854
BspCNI CTCAG 4 cut(s) 736, 871, 1002, 1094
BspFNI CGCG 1 cut(s) 130
BspHI TCATGA 1 cut(s) 670
BspLI GGNNCC 4 cut(s) 180, 255, 291, 545
BspPI GGATC 1 cut(s) 46
BspTI CTTAAG 1 cut(s) 329
BsrDI GCAATG 1 cut(s) 786
BsrGI TGTACA 1 cut(s) 381
BsrI ACTGG 2 cut(s) 255, 491
BssECI CCNNGG 2 cut(s) 84, 476
BssMI GATC 3 cut(s) 38, 550, 1087
BssT1I CCWWGG 2 cut(s) 84, 476
Bst4CI ACNGT 6 cut(s) 210, 221, 377, 616, 699, 736
Bst6I CTCTTC 1 cut(s) 917
BstAFI CTTAAG 1 cut(s) 329
BstAPI GCANNNNNTGC 2 cut(s) 172, 320
BstAUI TGTACA 1 cut(s) 381
BstDEI CTNAG 4 cut(s) 723, 879, 1010, 1081
BstDSI CCRYGG 1 cut(s) 476
BstENI CCTNNNNNAGG 1 cut(s) 107
BstF5I GGATG 2 cut(s) 197, 1126
BstFNI CGCG 1 cut(s) 130
BstHHI GCGC 1 cut(s) 793
BstKTI GATC 3 cut(s) 41, 553, 1090
BstMAI GTCTC 2 cut(s) 5, 725
BstMBI GATC 3 cut(s) 38, 550, 1087
BstMWI GCNNNNNNNGC 9 cut(s) 172, 178, 320, 580, 692, 851, 875, 884, 959
BstSCI CCNGG 1 cut(s) 301
BstSFI CTRYAG 1 cut(s) 612
BstSLI GKGCMC 1 cut(s) 514
BstUI CGCG 1 cut(s) 130
BstV1I GCAGC 7 cut(s) 159, 355, 594, 760, 829, 853, 1012
BstXI CCANNNNNNTGG 1 cut(s) 862
BsuRI GGCC 6 cut(s) 89, 102, 113, 181, 490, 854
BtgI CCRYGG 1 cut(s) 476
BtsCI GGATG 2 cut(s) 197, 1126
BtsIMutI CAGTG 2 cut(s) 248, 1007
CaiI CAGNNNCTG 1 cut(s) 320
CciI TCATGA 1 cut(s) 670
CfoI GCGC 1 cut(s) 793
Cfr13I GGNCC 2 cut(s) 81, 179
Csp6I GTAC 4 cut(s) 24, 382, 459, 534
CviAII CATG 2 cut(s) 477, 671
CviQI GTAC 4 cut(s) 24, 382, 459, 534
DdeI CTNAG 4 cut(s) 723, 879, 1010, 1081
DpnI GATC 3 cut(s) 40, 552, 1089
DpnII GATC 3 cut(s) 38, 550, 1087
DraI TTTAAA 1 cut(s) 108
DriI GACNNNNNGTC 1 cut(s) 802
EaeI YGGCCR 1 cut(s) 87
Eam1104I CTCTTC 1 cut(s) 917
Eam1105I GACNNNNNGTC 1 cut(s) 802
EarI CTCTTC 1 cut(s) 917
Eco130I CCWWGG 2 cut(s) 84, 476
Eco147I AGGCCT 1 cut(s) 102
Eco47I GGWCC 1 cut(s) 81
Eco57I CTGAAG 1 cut(s) 1088
EcoNI CCTNNNNNAGG 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 179
EcoT14I CCWWGG 2 cut(s) 84, 476
ErhI CCWWGG 2 cut(s) 84, 476
FaeI CATG 2 cut(s) 480, 674
FaiI YATR 8 cut(s) 269, 341, 363, 478, 501, 672, 702, 1044
FalI AAGNNNNNCTT 2 cut(s) 101, 133
FatI CATG 2 cut(s) 476, 670
Fnu4HI GCNGC 8 cut(s) 114, 173, 369, 608, 774, 843, 867, 1026
FokI GGATG 2 cut(s) 184, 1133
Fsp4HI GCNGC 8 cut(s) 114, 173, 369, 608, 774, 843, 867, 1026
FspBI CTAG 3 cut(s) 98, 524, 708
GlaI GCGC 1 cut(s) 792
GluI GCNGC 8 cut(s) 114, 173, 369, 608, 774, 843, 867, 1026
GsaI CCCAGC 1 cut(s) 179
HaeIII GGCC 6 cut(s) 89, 102, 113, 181, 490, 854
HapII CCGG 3 cut(s) 303, 421, 995
HhaI GCGC 1 cut(s) 793
Hin1II CATG 2 cut(s) 480, 674
Hin6I GCGC 1 cut(s) 791
HinP1I GCGC 1 cut(s) 791
HincII GTYRAC 1 cut(s) 1126
HindII GTYRAC 1 cut(s) 1126
HinfI GANTC 4 cut(s) 17, 185, 625, 651
HpaII CCGG 3 cut(s) 303, 421, 995
HphI GGTGA 5 cut(s) 198, 233, 574, 644, 986
Hpy166II GTNNAC 6 cut(s) 151, 461, 512, 837, 901, 1126
Hpy188I TCNGA 6 cut(s) 258, 294, 624, 726, 938, 1084
Hpy188III TCNNGA 5 cut(s) 36, 452, 671, 1075, 1106
Hpy8I GTNNAC 6 cut(s) 151, 461, 512, 837, 901, 1126
HpyAV CCTTC 4 cut(s) 665, 669, 823, 1112
HpyCH4III ACNGT 6 cut(s) 210, 221, 377, 616, 699, 736
HpyCH4IV ACGT 2 cut(s) 234, 282
HpyCH4V TGCA 9 cut(s) 166, 314, 512, 695, 845, 869, 986, 1028, 1040
HpyF10VI GCNNNNNNNGC 9 cut(s) 172, 178, 320, 580, 692, 851, 875, 884, 959
HpyF3I CTNAG 4 cut(s) 723, 879, 1010, 1081
HpySE526I ACGT 2 cut(s) 234, 282
Hsp92II CATG 2 cut(s) 480, 674
HspAI GCGC 1 cut(s) 791
Kzo9I GATC 3 cut(s) 38, 550, 1087
LmnI GCTCC 2 cut(s) 365, 543
Lsp1109I GCAGC 7 cut(s) 159, 355, 594, 760, 829, 853, 1012
LweI GCATC 3 cut(s) 175, 203, 409
MaeI CTAG 3 cut(s) 98, 524, 708
MaeII ACGT 2 cut(s) 234, 282
MaeIII GTNAC 1 cut(s) 141
MalI GATC 3 cut(s) 40, 552, 1089
MboI GATC 3 cut(s) 38, 550, 1087
MboII GAAGA 2 cut(s) 934, 1027
MfeI CAATTG 1 cut(s) 846
MhlI GDGCHC 1 cut(s) 514
MlyI GAGTC 2 cut(s) 619, 660
MmeI TCCRAC 1 cut(s) 59
MroXI GAANNNNTTC 1 cut(s) 833
MseI TTAA 4 cut(s) 107, 119, 285, 330
MspA1I CMGCKG 1 cut(s) 607
MspCI CTTAAG 1 cut(s) 329
MspI CCGG 3 cut(s) 303, 421, 995
MspR9I CCNGG 1 cut(s) 303
MunI CAATTG 1 cut(s) 846
MvnI CGCG 1 cut(s) 130
MwoI GCNNNNNNNGC 9 cut(s) 172, 178, 320, 580, 692, 851, 875, 884, 959
NciI CCSGG 1 cut(s) 303
NcoI CCATGG 1 cut(s) 476
NdeII GATC 3 cut(s) 38, 550, 1087
NlaIII CATG 2 cut(s) 480, 674
NlaIV GGNNCC 4 cut(s) 180, 255, 291, 545
PagI TCATGA 1 cut(s) 670
PceI AGGCCT 1 cut(s) 102
PdmI GAANNNNTTC 1 cut(s) 833
PfeI GAWTC 2 cut(s) 17, 185
PflFI GACNNNGTC 1 cut(s) 443
PkrI GCNGC 8 cut(s) 115, 174, 370, 609, 775, 844, 868, 1027
PleI GAGTC 2 cut(s) 619, 659
PpsI GAGTC 2 cut(s) 619, 659
Psp1406I AACGTT 1 cut(s) 282
PspFI CCCAGC 1 cut(s) 175
PspN4I GGNNCC 4 cut(s) 180, 255, 291, 545
PspPI GGNCC 2 cut(s) 81, 179
PstNI CAGNNNCTG 1 cut(s) 320
PsyI GACNNNGTC 1 cut(s) 443
PvuII CAGCTG 1 cut(s) 607
RsaI GTAC 4 cut(s) 25, 383, 460, 535
RsaNI GTAC 4 cut(s) 24, 382, 459, 534
SaqAI TTAA 4 cut(s) 107, 119, 285, 330
SatI GCNGC 8 cut(s) 114, 173, 369, 608, 774, 843, 867, 1026
Sau3AI GATC 3 cut(s) 38, 550, 1087
Sau96I GGNCC 2 cut(s) 81, 179
ScaI AGTACT 1 cut(s) 535
SchI GAGTC 2 cut(s) 619, 660
ScrFI CCNGG 1 cut(s) 303
SduI GDGCHC 1 cut(s) 514
SfaNI GCATC 3 cut(s) 175, 203, 409
SfcI CTRYAG 1 cut(s) 612
SinI GGWCC 1 cut(s) 81
SmlI CTYRAG 1 cut(s) 329
SmoI CTYRAG 1 cut(s) 329
SseBI AGGCCT 1 cut(s) 102
SsiI CCGC 3 cut(s) 114, 192, 1059
SspMI CTAG 3 cut(s) 98, 524, 708
StuI AGGCCT 1 cut(s) 102
StyD4I CCNGG 1 cut(s) 301
StyI CCWWGG 2 cut(s) 84, 476
TaaI ACNGT 6 cut(s) 210, 221, 377, 616, 699, 736
TaiI ACGT 2 cut(s) 237, 285
TaqI TCGA 4 cut(s) 390, 403, 577, 589
TatI WGTACW 2 cut(s) 381, 533
TauI GCSGC 1 cut(s) 116
TfiI GAWTC 2 cut(s) 17, 185
Tru1I TTAA 4 cut(s) 107, 119, 285, 330
Tru9I TTAA 4 cut(s) 107, 119, 285, 330
TscAI CASTG 2 cut(s) 255, 1014
TseI GCWGC 7 cut(s) 172, 368, 607, 773, 842, 866, 1025
TspDTI ATGAA 5 cut(s) 17, 284, 659, 687, 1127
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 2 cut(s) 255, 1014
Tth111I GACNNNGTC 1 cut(s) 443
Vha464I CTTAAG 1 cut(s) 329
VneI GTGCAC 1 cut(s) 510
VpaK11BI GGWCC 1 cut(s) 81
XagI CCTNNNNNAGG 1 cut(s) 107
XapI RAATTY 5 cut(s) 29, 63, 271, 1054, 1114
XcmI CCANNNNNNNNNTGG 1 cut(s) 895
XmnI GAANNNNTTC 1 cut(s) 833
XspI CTAG 3 cut(s) 98, 524, 708
ZrmI AGTACT 1 cut(s) 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.