pycom10g02030

Prohibitin-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0001997
Physical Location & Seq
Forward (+)
2130783 .. 2133500
2718 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g02030.1

Sequence Viewer

Length: 999 bp
ATGTTGGGCTGTGGAGATGAAACTTGTTCTCGCTCGTCGCCTGTAAACCCTAAACCCTTGCGTCTGCTACTGGGGTCTGAATCCCCACCGTCACGACCGCCACGCTCCTCACCTCTCACTGCGAGAAGGAGTATGAATTTCAAGAACGTTAATGTTAAGGTTCCGAAAGTTCCCGGTGGTGGTGCAATTTCTGCTTTGCTAATTGGTGGGCTTGGATTGTATGGAGCTGCCAACAGTCTGTACAATGTCGAGGGAGGGCATCGAGCCATTGTGTTCAACCGTATAGTCGGTGTCAAAGACAAGGTTTATCCGGAGGGGACACACCTTATCATTCCATGGTTTGAGAGGCCAATCATTTATGATGTCCGTGCACGACCTCATCTAGTGGAGAGTACTTCTGGGAGCCGTGATCTCCAAATGGTGAAAATTGGGCTTCGAGTGCTTACTCGACCTTTACCAGACCAGCTGCCTACAGTTTATCGGACTCTTGGTGAGAATTACAATGAGAGAGTCCTTCCTTCTATTGTTCATGAAACTTTGAAAGCTGTTGTTGCACAGTATAATGCTAGTCAACTCATTACTCAGAGAGAGACTGTTAGTAGGGAAATACGGAAGATTTTGACAGAAAGAGCTGCCAACTTCAACATTGCGCTGGATGATGTGTCAATTACCACCCTCACTTTTGGGAAGGAGTTCACGGCTGCAATTGAGGCCAAACAAGTGGCTGCACAGGAAGCTGAGAGAGCTAAGTTTGTTGTGGACAAGGCTGAACAAGACAAGAAGAGTGCTATCATCAGAGCAGAGGGAGAGGCCACTAGTGCCAAGCTGATTGGTGAAGCTATTGCGAACAATCCGGCATTTATAACACTGAGGAAGATTGAAGCTGCCAGAGAAATTGCGCATACAATCTCAAATTCAGCAAACAAAGTTTTCTTGAACTCGGATGATCTGTTGCTGAACCTTCAGGAGATGAACTTGGATATCAACAAGAAGAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Band_7

Protein Analysis

333

Amino Acids

36.44

Weight (kDa)

9.55

Isoelectric Point (pI)

40.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 82 - 259 1.4e-31 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 863
Acc16I TGCGCA 1 cut(s) 900
AccIII TCCGGA 1 cut(s) 310
AciI CCGC 1 cut(s) 98
AclI AACGTT 1 cut(s) 147
AcsI RAATTY 2 cut(s) 136, 913
AcuI CTGAAG 1 cut(s) 947
AfaI GTAC 2 cut(s) 242, 394
AfiI CCNNNNNNNGG 1 cut(s) 179
AgsI TTSAA 6 cut(s) 142, 277, 541, 643, 881, 937
AhlI ACTAGT 1 cut(s) 815
AluBI AGCT 9 cut(s) 227, 466, 545, 632, 737, 746, 826, 839, 884
AluI AGCT 9 cut(s) 227, 466, 545, 632, 737, 746, 826, 839, 884
Alw21I GWGCWC 1 cut(s) 373
Alw26I GTCTC 1 cut(s) 584
Alw44I GTGCAC 1 cut(s) 369
Aor13HI TCCGGA 1 cut(s) 310
AoxI GGCC 3 cut(s) 347, 711, 810
ApaLI GTGCAC 1 cut(s) 369
ApeKI GCWGC 6 cut(s) 227, 466, 632, 701, 725, 884
ApoI RAATTY 2 cut(s) 136, 913
Asp700I GAANNNNTTC 1 cut(s) 692
AspLEI GCGC 2 cut(s) 652, 901
AsuC2I CCSGG 1 cut(s) 174
AsuHPI GGTGA 4 cut(s) 102, 433, 503, 845
BaeGI GKGCMC 1 cut(s) 373
Bbv12I GWGCWC 1 cut(s) 373
BbvI GCAGC 6 cut(s) 214, 453, 619, 688, 712, 871
BceAI ACGGC 2 cut(s) 390, 714
BcnI CCSGG 1 cut(s) 174
BcoDI GTCTC 1 cut(s) 584
BcuI ACTAGT 1 cut(s) 815
BfaI CTAG 3 cut(s) 383, 567, 816
BfmI CTRYAG 1 cut(s) 471
BisI GCNGC 6 cut(s) 228, 467, 633, 702, 726, 885
BlsI GCNGC 6 cut(s) 229, 468, 634, 703, 727, 886
BmcAI AGTACT 1 cut(s) 394
Bme1390I CCNGG 1 cut(s) 174
BmiI GGNNCC 2 cut(s) 162, 404
BmrFI CCNGG 1 cut(s) 174
BmrI ACTGGG 1 cut(s) 80
BmsI GCATC 1 cut(s) 268
BmuI ACTGGG 1 cut(s) 80
BpuMI CCSGG 1 cut(s) 174
BsaJI CCNNGG 1 cut(s) 335
BsaWI WCCGGW 1 cut(s) 310
Bsc4I CCNNNNNNNGG 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 75
Bse3DI GCAATG 1 cut(s) 645
BseAI TCCGGA 1 cut(s) 310
BseDI CCNNGG 1 cut(s) 335
BseGI GGATG 2 cut(s) 661, 949
BseLI CCNNNNNNNGG 1 cut(s) 179
BseMI GCAATG 1 cut(s) 645
BseMII CTCAG 3 cut(s) 596, 729, 860
BseNI ACTGG 1 cut(s) 75
BseRI GAGGAG 1 cut(s) 97
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 6 cut(s) 214, 453, 619, 688, 712, 871
BsgI GTGCAG 1 cut(s) 711
Bsh1285I CGRYCG 1 cut(s) 98
BshFI GGCC 3 cut(s) 349, 713, 812
BsiEI CGRYCG 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 373
BsiSI CCGG 3 cut(s) 174, 311, 854
BslFI GGGAC 1 cut(s) 331
BslI CCNNNNNNNGG 1 cut(s) 179
BsmAI GTCTC 1 cut(s) 584
BsmFI GGGAC 1 cut(s) 331
BsnI GGCC 3 cut(s) 349, 713, 812
Bsp1286I GDGCHC 1 cut(s) 373
Bsp13I TCCGGA 1 cut(s) 310
Bsp1407I TGTACA 1 cut(s) 240
Bsp143I GATC 2 cut(s) 409, 946
Bsp19I CCATGG 1 cut(s) 335
BspACI CCGC 1 cut(s) 98
BspANI GGCC 3 cut(s) 349, 713, 812
BspCNI CTCAG 3 cut(s) 595, 730, 861
BspEI TCCGGA 1 cut(s) 310
BspHI TCATGA 1 cut(s) 529
BspLI GGNNCC 2 cut(s) 162, 404
BsrDI GCAATG 1 cut(s) 645
BsrGI TGTACA 1 cut(s) 240
BsrI ACTGG 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 335
BssMI GATC 2 cut(s) 409, 946
BssT1I CCWWGG 1 cut(s) 335
Bst4CI ACNGT 6 cut(s) 90, 236, 281, 475, 558, 595
Bst6I CTCTTC 1 cut(s) 776
BstAPI GCANNNNNTGC 1 cut(s) 191
BstAUI TGTACA 1 cut(s) 240
BstDEI CTNAG 4 cut(s) 582, 738, 747, 869
BstDSI CCRYGG 1 cut(s) 335
BstF5I GGATG 2 cut(s) 661, 949
BstHHI GCGC 2 cut(s) 652, 901
BstKTI GATC 2 cut(s) 412, 949
BstMAI GTCTC 1 cut(s) 584
BstMBI GATC 2 cut(s) 409, 946
BstMCI CGRYCG 1 cut(s) 98
BstMWI GCNNNNNNNGC 7 cut(s) 191, 439, 551, 710, 734, 743, 818
BstSCI CCNGG 1 cut(s) 172
BstSFI CTRYAG 1 cut(s) 471
BstSLI GKGCMC 1 cut(s) 373
BstV1I GCAGC 6 cut(s) 214, 453, 619, 688, 712, 871
BstXI CCANNNNNNTGG 1 cut(s) 721
BsuRI GGCC 3 cut(s) 349, 713, 812
BtgI CCRYGG 1 cut(s) 335
BtsCI GGATG 2 cut(s) 661, 949
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 2 cut(s) 117, 866
CciI TCATGA 1 cut(s) 529
CfoI GCGC 2 cut(s) 652, 901
CseI GACGC 1 cut(s) 50
Csp6I GTAC 2 cut(s) 241, 393
CviAII CATG 2 cut(s) 336, 530
CviQI GTAC 2 cut(s) 241, 393
DdeI CTNAG 4 cut(s) 582, 738, 747, 869
DpnI GATC 2 cut(s) 411, 948
DpnII GATC 2 cut(s) 409, 946
Eam1104I CTCTTC 1 cut(s) 776
EarI CTCTTC 1 cut(s) 776
Eco130I CCWWGG 1 cut(s) 335
Eco32I GATATC 1 cut(s) 982
Eco57I CTGAAG 1 cut(s) 947
EcoRV GATATC 1 cut(s) 982
EcoT14I CCWWGG 1 cut(s) 335
ErhI CCWWGG 1 cut(s) 335
FaeI CATG 2 cut(s) 339, 533
FaiI YATR 9 cut(s) 134, 222, 284, 337, 360, 531, 561, 863, 903
FaqI GGGAC 1 cut(s) 331
FatI CATG 2 cut(s) 335, 529
Fnu4HI GCNGC 6 cut(s) 228, 467, 633, 702, 726, 885
FokI GGATG 2 cut(s) 668, 956
Fsp4HI GCNGC 6 cut(s) 228, 467, 633, 702, 726, 885
FspBI CTAG 3 cut(s) 383, 567, 816
FspI TGCGCA 1 cut(s) 900
GlaI GCGC 2 cut(s) 651, 900
GluI GCNGC 6 cut(s) 228, 467, 633, 702, 726, 885
HaeIII GGCC 3 cut(s) 349, 713, 812
HapII CCGG 3 cut(s) 174, 311, 854
HgaI GACGC 1 cut(s) 50
HhaI GCGC 2 cut(s) 652, 901
Hin1II CATG 2 cut(s) 339, 533
Hin6I GCGC 2 cut(s) 650, 899
HinP1I GCGC 2 cut(s) 650, 899
HincII GTYRAC 1 cut(s) 572
HindII GTYRAC 1 cut(s) 572
HinfI GANTC 3 cut(s) 80, 484, 510
HpaII CCGG 3 cut(s) 174, 311, 854
HphI GGTGA 4 cut(s) 102, 433, 503, 845
Hpy166II GTNNAC 5 cut(s) 46, 371, 572, 696, 760
Hpy188I TCNGA 6 cut(s) 79, 165, 483, 585, 797, 943
Hpy188III TCNNGA 6 cut(s) 93, 142, 311, 530, 934, 965
Hpy8I GTNNAC 5 cut(s) 46, 371, 572, 696, 760
Hpy99I CGWCG 1 cut(s) 40
HpyAV CCTTC 5 cut(s) 120, 524, 528, 682, 971
HpyCH4III ACNGT 6 cut(s) 90, 236, 281, 475, 558, 595
HpyCH4IV ACGT 1 cut(s) 147
HpyCH4V TGCA 5 cut(s) 185, 371, 554, 704, 728
HpyF10VI GCNNNNNNNGC 7 cut(s) 191, 439, 551, 710, 734, 743, 818
HpyF3I CTNAG 4 cut(s) 582, 738, 747, 869
HpySE526I ACGT 1 cut(s) 147
Hsp92II CATG 2 cut(s) 339, 533
HspAI GCGC 2 cut(s) 650, 899
Kpn2I TCCGGA 1 cut(s) 310
Kzo9I GATC 2 cut(s) 409, 946
LmnI GCTCC 3 cut(s) 110, 224, 402
Lsp1109I GCAGC 6 cut(s) 214, 453, 619, 688, 712, 871
LweI GCATC 1 cut(s) 268
MaeI CTAG 3 cut(s) 383, 567, 816
MaeII ACGT 1 cut(s) 147
MaeIII GTNAC 1 cut(s) 90
MalI GATC 2 cut(s) 411, 948
MboI GATC 2 cut(s) 409, 946
MboII GAAGA 3 cut(s) 625, 793, 886
MfeI CAATTG 1 cut(s) 705
MhlI GDGCHC 1 cut(s) 373
MluCI AATT 9 cut(s) 136, 186, 201, 426, 496, 666, 705, 894, 913
MlyI GAGTC 2 cut(s) 478, 519
MroI TCCGGA 1 cut(s) 310
MroXI GAANNNNTTC 1 cut(s) 692
MseI TTAA 2 cut(s) 150, 156
MspA1I CMGCKG 1 cut(s) 466
MspI CCGG 3 cut(s) 174, 311, 854
MspR9I CCNGG 1 cut(s) 174
MunI CAATTG 1 cut(s) 705
MwoI GCNNNNNNNGC 7 cut(s) 191, 439, 551, 710, 734, 743, 818
NciI CCSGG 1 cut(s) 174
NcoI CCATGG 1 cut(s) 335
NdeII GATC 2 cut(s) 409, 946
NlaIII CATG 2 cut(s) 339, 533
NlaIV GGNNCC 2 cut(s) 162, 404
NmuCI GTSAC 1 cut(s) 90
NsbI TGCGCA 1 cut(s) 900
PagI TCATGA 1 cut(s) 529
PdmI GAANNNNTTC 1 cut(s) 692
PfeI GAWTC 1 cut(s) 80
PkrI GCNGC 6 cut(s) 229, 468, 634, 703, 727, 886
PleI GAGTC 2 cut(s) 478, 518
PpsI GAGTC 2 cut(s) 478, 518
PsiI TTATAA 1 cut(s) 863
Psp1406I AACGTT 1 cut(s) 147
PspN4I GGNNCC 2 cut(s) 162, 404
PvuII CAGCTG 1 cut(s) 466
RsaI GTAC 2 cut(s) 242, 394
RsaNI GTAC 2 cut(s) 241, 393
SaqAI TTAA 2 cut(s) 150, 156
SatI GCNGC 6 cut(s) 228, 467, 633, 702, 726, 885
Sau3AI GATC 2 cut(s) 409, 946
ScaI AGTACT 1 cut(s) 394
SchI GAGTC 2 cut(s) 478, 519
ScrFI CCNGG 1 cut(s) 174
SduI GDGCHC 1 cut(s) 373
SfaNI GCATC 1 cut(s) 268
SfcI CTRYAG 1 cut(s) 471
SpeI ACTAGT 1 cut(s) 815
Sse9I AATT 9 cut(s) 136, 186, 201, 426, 496, 666, 705, 894, 913
SsiI CCGC 1 cut(s) 98
SspMI CTAG 3 cut(s) 383, 567, 816
StyD4I CCNGG 1 cut(s) 172
StyI CCWWGG 1 cut(s) 335
TaaI ACNGT 6 cut(s) 90, 236, 281, 475, 558, 595
TaiI ACGT 1 cut(s) 150
TaqI TCGA 4 cut(s) 249, 262, 436, 448
TasI AATT 9 cut(s) 136, 186, 201, 426, 496, 666, 705, 894, 913
TatI WGTACW 2 cut(s) 240, 392
TfiI GAWTC 1 cut(s) 80
Tru1I TTAA 2 cut(s) 150, 156
Tru9I TTAA 2 cut(s) 150, 156
TscAI CASTG 2 cut(s) 124, 873
TseFI GTSAC 1 cut(s) 90
TseI GCWGC 6 cut(s) 227, 466, 632, 701, 725, 884
Tsp45I GTSAC 1 cut(s) 90
TspDTI ATGAA 5 cut(s) 33, 149, 518, 546, 986
TspGWI ACGGA 2 cut(s) 356, 625
TspRI CASTG 2 cut(s) 124, 873
VneI GTGCAC 1 cut(s) 369
XapI RAATTY 2 cut(s) 136, 913
XmnI GAANNNNTTC 1 cut(s) 692
XspI CTAG 3 cut(s) 383, 567, 816
ZrmI AGTACT 1 cut(s) 394
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.