Rw5G025880

Prohibitin-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001997
Physical Location & Seq
Forward (+)
37069871 .. 37074048
4178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G025880.1

Sequence Viewer

Length: 858 bp
ATGAATTTCAACAAGAATATGAAAGTTCCGAAAGTGCCAGTTCGCGGGATTGGTACTTTGCTTACTGTGGGAGTTATTGGTGGTCTTGGTGTTTATGGACTTAACAATGGTCTCTACAATGTTGAAGGAGGGAGCCGGGCTATTGTGTTCAACCGCATTACTGGTGTCAAAGACAAGGTCTATCCGGAAGGGACACACCTAATAATTCCATGGTTTGAGAGGCCAATCATCTATGATGTCCGCGCACGACCCCATTTTGTGGACAGCATTGCTGGAAGCCGTGACCTCCAGATGGTGAAAATAGGGCTTCGAGTTCTGGTCCGTCCTGTGCCTGACCAATTACCTACAATTTATCGTACTTTAGGTGTGGATTATCTTGACAGGATCATGAAGTCAATCATTCATGAAACTTTGAAAGCTGTAGTTGCACAATATAATGCTAGCCAACTGATTACTCAGAGAGAGACTGTTAGTAGGGAAATTCGGAAGATTTTGACAGAGAGGGCAGCCAAGTTCAATATTGCAATGGATGATGTATCTATTACCAGCCTGACTTTTGGGAAGGAGTTTATGATTGCAATTGAAGCGAAACAAGTAGCTGCTCAAGAAGCTGAGAGGGCCAAGTATGTTGTGGAGAAAGCTGAACAAGACAAGAAAAGTGCTATTATCAGAGCCGAGGGTGAAGCAAAAAGTGCCCAACTTATAGGTGAAGCCACTGCCAAGAACCCAGCATTTGTCACTCTAAGGAAGATTGAAGCTGGAAGAGAAATTGCACAAATCATCTCCAATTCAGATAATAAAGTTTATCTTAACTCCGATAGTCTGTTGCTCAACCTTCAGGAGTTGAATTTGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Band_7

Protein Analysis

285

Amino Acids

31.66

Weight (kDa)

9.5

Isoelectric Point (pI)

28.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 41 - 217 9.8e-31 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 259
AccII CGCG 2 cut(s) 45, 243
AccIII TCCGGA 1 cut(s) 184
AciI CCGC 3 cut(s) 45, 154, 241
AclWI GGATC 1 cut(s) 392
AcsI RAATTY 3 cut(s) 4, 480, 847
AcuI CTGAAG 1 cut(s) 821
AfaI GTAC 2 cut(s) 55, 358
AfiI CCNNNNNNNGG 3 cut(s) 44, 259, 292
AgsI TTSAA 8 cut(s) 10, 125, 151, 415, 517, 584, 755, 847
AluBI AGCT 5 cut(s) 419, 599, 611, 641, 758
AluI AGCT 5 cut(s) 419, 599, 611, 641, 758
Alw26I GTCTC 2 cut(s) 116, 458
AlwI GGATC 1 cut(s) 392
Aor13HI TCCGGA 1 cut(s) 184
AoxI GGCC 2 cut(s) 221, 618
ApeKI GCWGC 2 cut(s) 506, 599
ApoI RAATTY 3 cut(s) 4, 480, 847
ArsI GACNNNNNNTTYG 2 cut(s) 162, 194
AspLEI GCGC 1 cut(s) 245
AspS9I GGNCC 2 cut(s) 319, 618
AsuC2I CCSGG 1 cut(s) 137
AsuHPI GGTGA 3 cut(s) 307, 692, 719
AsuNHI GCTAGC 1 cut(s) 440
AvaII GGWCC 1 cut(s) 319
BaeGI GKGCMC 1 cut(s) 697
BbvI GCAGC 2 cut(s) 518, 586
BccI CCATC 1 cut(s) 286
BceAI ACGGC 1 cut(s) 264
BcnI CCSGG 1 cut(s) 137
BcoDI GTCTC 2 cut(s) 116, 458
BfaI CTAG 1 cut(s) 441
BfmI CTRYAG 1 cut(s) 420
BisI GCNGC 2 cut(s) 507, 600
BlsI GCNGC 2 cut(s) 508, 601
Bme1390I CCNGG 1 cut(s) 137
Bme18I GGWCC 1 cut(s) 319
BmgT120I GGNCC 2 cut(s) 319, 618
BmiI GGNNCC 1 cut(s) 134
BmrFI CCNGG 1 cut(s) 137
BmtI GCTAGC 1 cut(s) 444
BpmI CTGGAG 1 cut(s) 272
BpuEI CTTGAG 1 cut(s) 588
BpuMI CCSGG 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 116
BsaJI CCNNGG 2 cut(s) 209, 675
BsaWI WCCGGW 1 cut(s) 184
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 259, 292
Bse1I ACTGG 2 cut(s) 38, 166
Bse3DI GCAATG 2 cut(s) 267, 531
BseAI TCCGGA 1 cut(s) 184
BseDI CCNNGG 2 cut(s) 209, 675
BseGI GGATG 1 cut(s) 535
BseLI CCNNNNNNNGG 3 cut(s) 44, 259, 292
BseMI GCAATG 2 cut(s) 267, 531
BseMII CTCAG 2 cut(s) 470, 603
BseNI ACTGG 2 cut(s) 38, 166
BseSI GKGCMC 1 cut(s) 697
BseXI GCAGC 2 cut(s) 518, 586
BseYI CCCAGC 1 cut(s) 727
Bsh1236I CGCG 2 cut(s) 45, 243
BshFI GGCC 2 cut(s) 223, 620
BsiSI CCGG 2 cut(s) 136, 185
BslFI GGGAC 1 cut(s) 205
BslI CCNNNNNNNGG 3 cut(s) 44, 259, 292
BsmAI GTCTC 2 cut(s) 116, 458
BsmFI GGGAC 1 cut(s) 205
BsnI GGCC 2 cut(s) 223, 620
Bso31I GGTCTC 1 cut(s) 116
Bsp1286I GDGCHC 1 cut(s) 697
Bsp13I TCCGGA 1 cut(s) 184
Bsp143I GATC 1 cut(s) 384
Bsp19I CCATGG 1 cut(s) 209
BspACI CCGC 3 cut(s) 45, 154, 241
BspANI GGCC 2 cut(s) 223, 620
BspCNI CTCAG 2 cut(s) 469, 604
BspEI TCCGGA 1 cut(s) 184
BspFNI CGCG 2 cut(s) 45, 243
BspHI TCATGA 2 cut(s) 387, 403
BspLI GGNNCC 1 cut(s) 134
BspOI GCTAGC 1 cut(s) 444
BspPI GGATC 1 cut(s) 392
BspTNI GGTCTC 1 cut(s) 116
BsrDI GCAATG 2 cut(s) 267, 531
BsrI ACTGG 2 cut(s) 38, 166
BssECI CCNNGG 2 cut(s) 209, 675
BssMI GATC 1 cut(s) 384
BssT1I CCWWGG 1 cut(s) 209
Bst4CI ACNGT 2 cut(s) 67, 469
Bst6I CTCTTC 1 cut(s) 757
BstAPI GCANNNNNTGC 1 cut(s) 692
BstC8I GCNNGC 1 cut(s) 442
BstDEI CTNAG 3 cut(s) 456, 612, 743
BstDSI CCRYGG 1 cut(s) 209
BstF5I GGATG 1 cut(s) 535
BstFNI CGCG 2 cut(s) 45, 243
BstHHI GCGC 1 cut(s) 245
BstKTI GATC 1 cut(s) 387
BstMAI GTCTC 2 cut(s) 116, 458
BstMBI GATC 1 cut(s) 384
BstMWI GCNNNNNNNGC 5 cut(s) 425, 584, 608, 617, 692
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 1 cut(s) 420
BstSLI GKGCMC 1 cut(s) 697
BstUI CGCG 2 cut(s) 45, 243
BstV1I GCAGC 2 cut(s) 518, 586
BsuRI GGCC 2 cut(s) 223, 620
BtgI CCRYGG 1 cut(s) 209
BtsCI GGATG 1 cut(s) 535
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 1 cut(s) 714
Cac8I GCNNGC 1 cut(s) 442
CciI TCATGA 2 cut(s) 387, 403
CfoI GCGC 1 cut(s) 245
Cfr13I GGNCC 2 cut(s) 319, 618
Csp6I GTAC 2 cut(s) 54, 357
CviAII CATG 3 cut(s) 210, 388, 404
CviQI GTAC 2 cut(s) 54, 357
DdeI CTNAG 3 cut(s) 456, 612, 743
DpnI GATC 1 cut(s) 386
DpnII GATC 1 cut(s) 384
Eam1104I CTCTTC 1 cut(s) 757
EarI CTCTTC 1 cut(s) 757
Eco130I CCWWGG 1 cut(s) 209
Eco31I GGTCTC 1 cut(s) 116
Eco47I GGWCC 1 cut(s) 319
Eco57I CTGAAG 1 cut(s) 821
EcoT14I CCWWGG 1 cut(s) 209
ErhI CCWWGG 1 cut(s) 209
FaeI CATG 3 cut(s) 213, 391, 407
FalI AAGNNNNNCTT 2 cut(s) 792, 824
FaqI GGGAC 1 cut(s) 205
FatI CATG 3 cut(s) 209, 387, 403
FauI CCCGC 1 cut(s) 38
Fnu4HI GCNGC 2 cut(s) 507, 600
FokI GGATG 1 cut(s) 542
Fsp4HI GCNGC 2 cut(s) 507, 600
FspBI CTAG 1 cut(s) 441
GlaI GCGC 1 cut(s) 244
GluI GCNGC 2 cut(s) 507, 600
GsaI CCCAGC 1 cut(s) 731
GsuI CTGGAG 1 cut(s) 272
HaeIII GGCC 2 cut(s) 223, 620
HapII CCGG 2 cut(s) 136, 185
HhaI GCGC 1 cut(s) 245
Hin1II CATG 3 cut(s) 213, 391, 407
Hin6I GCGC 1 cut(s) 243
HinP1I GCGC 1 cut(s) 243
HpaII CCGG 2 cut(s) 136, 185
HphI GGTGA 3 cut(s) 307, 692, 719
Hpy166II GTNNAC 1 cut(s) 262
Hpy188I TCNGA 6 cut(s) 30, 459, 486, 671, 793, 817
Hpy188III TCNNGA 7 cut(s) 185, 289, 377, 388, 404, 605, 839
Hpy8I GTNNAC 1 cut(s) 262
HpyAV CCTTC 4 cut(s) 119, 182, 556, 845
HpyCH4III ACNGT 2 cut(s) 67, 469
HpyCH4V TGCA 4 cut(s) 428, 524, 578, 773
HpyF10VI GCNNNNNNNGC 5 cut(s) 425, 584, 608, 617, 692
HpyF3I CTNAG 3 cut(s) 456, 612, 743
Hsp92II CATG 3 cut(s) 213, 391, 407
HspAI GCGC 1 cut(s) 243
Kpn2I TCCGGA 1 cut(s) 184
Kzo9I GATC 1 cut(s) 384
LmnI GCTCC 1 cut(s) 132
Lsp1109I GCAGC 2 cut(s) 518, 586
MaeI CTAG 1 cut(s) 441
MaeIII GTNAC 2 cut(s) 281, 736
MalI GATC 1 cut(s) 386
MboI GATC 1 cut(s) 384
MboII GAAGA 3 cut(s) 499, 760, 774
MfeI CAATTG 1 cut(s) 579
MhlI GDGCHC 1 cut(s) 697
MluCI AATT 9 cut(s) 4, 204, 338, 348, 480, 579, 768, 787, 847
MnlI CCTC 6 cut(s) 122, 213, 296, 495, 609, 670
MroI TCCGGA 1 cut(s) 184
MseI TTAA 3 cut(s) 102, 810, 856
MspI CCGG 2 cut(s) 136, 185
MspR9I CCNGG 1 cut(s) 137
MunI CAATTG 1 cut(s) 579
MvnI CGCG 2 cut(s) 45, 243
MwoI GCNNNNNNNGC 5 cut(s) 425, 584, 608, 617, 692
NciI CCSGG 1 cut(s) 137
NcoI CCATGG 1 cut(s) 209
NdeII GATC 1 cut(s) 384
NheI GCTAGC 1 cut(s) 440
NlaIII CATG 3 cut(s) 213, 391, 407
NlaIV GGNNCC 1 cut(s) 134
NmeAIII GCCGAG 1 cut(s) 700
NmuCI GTSAC 2 cut(s) 281, 736
PagI TCATGA 2 cut(s) 387, 403
PflFI GACNNNGTC 1 cut(s) 176
PflMI CCANNNNNTGG 1 cut(s) 259
PkrI GCNGC 2 cut(s) 508, 601
PspFI CCCAGC 1 cut(s) 727
PspN4I GGNNCC 1 cut(s) 134
PspPI GGNCC 2 cut(s) 319, 618
PsyI GACNNNGTC 1 cut(s) 176
RsaI GTAC 2 cut(s) 55, 358
RsaNI GTAC 2 cut(s) 54, 357
SaqAI TTAA 3 cut(s) 102, 810, 856
SatI GCNGC 2 cut(s) 507, 600
Sau3AI GATC 1 cut(s) 384
Sau96I GGNCC 2 cut(s) 319, 618
ScrFI CCNGG 1 cut(s) 137
SduI GDGCHC 1 cut(s) 697
SfcI CTRYAG 1 cut(s) 420
SinI GGWCC 1 cut(s) 319
SmlI CTYRAG 1 cut(s) 603
SmoI CTYRAG 1 cut(s) 603
Sse9I AATT 9 cut(s) 4, 204, 338, 348, 480, 579, 768, 787, 847
SsiI CCGC 3 cut(s) 45, 154, 241
SspI AATATT 1 cut(s) 520
SspMI CTAG 1 cut(s) 441
StyD4I CCNGG 1 cut(s) 135
StyI CCWWGG 1 cut(s) 209
TaaI ACNGT 2 cut(s) 67, 469
TaqI TCGA 1 cut(s) 310
TasI AATT 9 cut(s) 4, 204, 338, 348, 480, 579, 768, 787, 847
Tru1I TTAA 3 cut(s) 102, 810, 856
Tru9I TTAA 3 cut(s) 102, 810, 856
TscAI CASTG 1 cut(s) 721
TseFI GTSAC 2 cut(s) 281, 736
TseI GCWGC 2 cut(s) 506, 599
Tsp45I GTSAC 2 cut(s) 281, 736
TspDTI ATGAA 5 cut(s) 17, 35, 392, 404, 420
TspGWI ACGGA 1 cut(s) 311
TspRI CASTG 1 cut(s) 721
Tth111I GACNNNGTC 1 cut(s) 176
Van91I CCANNNNNTGG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 319
XapI RAATTY 3 cut(s) 4, 480, 847
XcmI CCANNNNNNNNNTGG 1 cut(s) 628
XspI CTAG 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.