MD11G1212000.v1.1

Prohibitin-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001997
Physical Location & Seq
Reverse (-)
30944221 .. 30948360
4140 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1212000.v1.1.491

Sequence Viewer

Length: 840 bp
ATGAAAGTCCCCAAACTTCCTGGAGCTCGTGGCATTGCAACTTTGCTTACGGTGGGAGTTGTTGGTAGTCTTGGTGTATATGGACTTAACAATAGTCTCTACAATGTCGATGGAGGGCATAGGTCCATTGTGTTCAGTCGTCTTGTTGGTGTCAAAGACAAGGTTTATCCTGAAGGGACACACCTTATAATTCCATGGTTTGAAAGGCCGGTCATCTATGATGTTCGTGCAAAACCTTATGTAGTGGAGAGTAAAGCTGGAAGCCGTGACCTCCAGATGGTATTAGTAGGTCTTCGAGTGCTGACCCGTCCCGTGCCAGACGAATTACCTACAGTTTATCGAACTCTGGGTGAGAATTATGTTGAGAGGGTCATGCCTTCAATTATCCATGAAACTTTGAAAGCTGTAGTTGCACAGTATAATGCCAGCCAACTTATTACTCAGAGAGAGGCCGTCAGTAGGGAGATACGGAAAATTTTGACAGAGAGGGCAGCGAATTTTAACATTGCACTGGATGATGTGTCTATCACTAACCTGACTTTTGGAAAGGAGTTTCTGACTGCAATTGAAGCAAAACAGGTAGCTGCACAAGAGGCTGAGAGGGCAAAATTTGTTGTGGAGAAAGCCGAACAAGACAAGAAAAGTGCTGTTATCAGAGCAGAGGGTGAAGCAACGAGTGCGAAGCTGATTGGTCAAGCCATTGCCAACAATCCAGCATTTATCACTCTACGGAAGATTGAGGCTGCAAGAGAAATCGCCCAGGTTATCTCAGATTCCAAAAACATGGTTTACTTGAACGCCGATGAACTTTTGCTGAACATTCAGGGGAATGTGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Band_7

Protein Analysis

280

Amino Acids

30.72

Weight (kDa)

9.11

Isoelectric Point (pI)

29.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 36 - 212 1.5e-31 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 188
AcsI RAATTY 3 cut(s) 474, 496, 608
AcuI CTGAAG 1 cut(s) 192
AfiI CCNNNNNNNGG 2 cut(s) 277, 459
AgsI TTSAA 5 cut(s) 203, 381, 400, 569, 796
AjnI CCWGG 2 cut(s) 19, 759
AluBI AGCT 5 cut(s) 26, 257, 404, 584, 685
AluI AGCT 5 cut(s) 26, 257, 404, 584, 685
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 1 cut(s) 101
AoxI GGCC 2 cut(s) 206, 450
ApeKI GCWGC 3 cut(s) 491, 584, 743
ApoI RAATTY 3 cut(s) 474, 496, 608
AspS9I GGNCC 1 cut(s) 123
AsuHPI GGTGA 2 cut(s) 362, 677
AvaII GGWCC 1 cut(s) 123
BanII GRGCYC 1 cut(s) 28
BauI CACGAG 1 cut(s) 27
BbsI GAAGAC 1 cut(s) 284
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 3 cut(s) 503, 571, 730
BccI CCATC 2 cut(s) 104, 271
BceAI ACGGC 2 cut(s) 249, 437
BciT130I CCWGG 2 cut(s) 21, 761
BcoDI GTCTC 1 cut(s) 101
BfmI CTRYAG 2 cut(s) 330, 405
BisI GCNGC 3 cut(s) 492, 585, 744
BlsI GCNGC 3 cut(s) 493, 586, 745
Bme1390I CCNGG 2 cut(s) 21, 761
Bme18I GGWCC 1 cut(s) 123
BmgT120I GGNCC 1 cut(s) 123
BmrFI CCNGG 2 cut(s) 21, 761
BpiI GAAGAC 1 cut(s) 284
BpmI CTGGAG 2 cut(s) 42, 257
BsaJI CCNNGG 2 cut(s) 194, 759
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 459
Bse118I RCCGGY 1 cut(s) 208
Bse1I ACTGG 1 cut(s) 516
Bse3DI GCAATG 3 cut(s) 33, 504, 699
BseBI CCWGG 2 cut(s) 21, 761
BseDI CCNNGG 2 cut(s) 194, 759
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 2 cut(s) 277, 459
BseMI GCAATG 3 cut(s) 33, 504, 699
BseMII CTCAG 3 cut(s) 455, 588, 783
BseNI ACTGG 1 cut(s) 516
BseXI GCAGC 3 cut(s) 503, 571, 730
BsgI GTGCAG 1 cut(s) 570
BshFI GGCC 2 cut(s) 208, 452
BsiHKAI GWGCWC 1 cut(s) 28
BsiSI CCGG 1 cut(s) 209
BslFI GGGAC 2 cut(s) 190, 294
BslI CCNNNNNNNGG 2 cut(s) 277, 459
BsmAI GTCTC 1 cut(s) 101
BsmFI GGGAC 2 cut(s) 190, 294
BsnI GGCC 2 cut(s) 208, 452
Bsp1286I GDGCHC 1 cut(s) 28
Bsp19I CCATGG 1 cut(s) 194
BspANI GGCC 2 cut(s) 208, 452
BspCNI CTCAG 3 cut(s) 454, 589, 782
BsrDI GCAATG 3 cut(s) 33, 504, 699
BsrFI RCCGGY 1 cut(s) 208
BsrI ACTGG 1 cut(s) 516
BssAI RCCGGY 1 cut(s) 208
BssECI CCNNGG 2 cut(s) 194, 759
BssSI CACGAG 1 cut(s) 27
BssT1I CCWWGG 1 cut(s) 194
Bst2BI CACGAG 1 cut(s) 27
Bst2UI CCWGG 2 cut(s) 21, 761
Bst4CI ACNGT 3 cut(s) 52, 334, 417
BstAPI GCANNNNNTGC 1 cut(s) 677
BstC8I GCNNGC 1 cut(s) 427
BstDEI CTNAG 3 cut(s) 441, 597, 769
BstDSI CCRYGG 1 cut(s) 194
BstF5I GGATG 1 cut(s) 520
BstMAI GTCTC 1 cut(s) 101
BstMWI GCNNNNNNNGC 5 cut(s) 410, 569, 593, 602, 677
BstNI CCWGG 2 cut(s) 21, 761
BstSCI CCNGG 2 cut(s) 19, 759
BstSFI CTRYAG 2 cut(s) 330, 405
BstV1I GCAGC 3 cut(s) 503, 571, 730
BstV2I GAAGAC 1 cut(s) 284
BstXI CCANNNNNNTGG 1 cut(s) 784
BsuRI GGCC 2 cut(s) 208, 452
BtgI CCRYGG 1 cut(s) 194
BtsCI GGATG 1 cut(s) 520
BtsIMutI CAGTG 1 cut(s) 509
Cac8I GCNNGC 1 cut(s) 427
Cfr10I RCCGGY 1 cut(s) 208
Cfr13I GGNCC 1 cut(s) 123
CviAII CATG 4 cut(s) 195, 373, 389, 784
DdeI CTNAG 3 cut(s) 441, 597, 769
Ecl136II GAGCTC 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 194
Eco24I GRGCYC 1 cut(s) 28
Eco47I GGWCC 1 cut(s) 123
Eco53kI GAGCTC 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 192
EcoICRI GAGCTC 1 cut(s) 26
EcoRII CCWGG 2 cut(s) 19, 759
EcoT14I CCWWGG 1 cut(s) 194
EcoT38I GRGCYC 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 194
FaeI CATG 4 cut(s) 198, 376, 392, 787
FaqI GGGAC 2 cut(s) 190, 294
FatI CATG 4 cut(s) 194, 372, 388, 783
Fnu4HI GCNGC 3 cut(s) 492, 585, 744
FokI GGATG 1 cut(s) 527
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 3 cut(s) 492, 585, 744
GluI GCNGC 3 cut(s) 492, 585, 744
GsuI CTGGAG 2 cut(s) 42, 257
HaeIII GGCC 2 cut(s) 208, 452
HapII CCGG 1 cut(s) 209
Hin1II CATG 4 cut(s) 198, 376, 392, 787
HinfI GANTC 1 cut(s) 773
HpaII CCGG 1 cut(s) 209
HphI GGTGA 2 cut(s) 362, 677
Hpy166II GTNNAC 1 cut(s) 790
Hpy188I TCNGA 4 cut(s) 444, 558, 656, 772
Hpy188III TCNNGA 2 cut(s) 170, 274
Hpy8I GTNNAC 1 cut(s) 790
HpyAV CCTTC 2 cut(s) 167, 387
HpyCH4III ACNGT 3 cut(s) 52, 334, 417
HpyCH4V TGCA 8 cut(s) 38, 230, 413, 509, 563, 587, 746, 835
HpyF10VI GCNNNNNNNGC 5 cut(s) 410, 569, 593, 602, 677
HpyF3I CTNAG 3 cut(s) 441, 597, 769
Hsp92II CATG 4 cut(s) 198, 376, 392, 787
LmnI GCTCC 1 cut(s) 23
Lsp1109I GCAGC 3 cut(s) 503, 571, 730
MaeIII GTNAC 1 cut(s) 266
MboII GAAGA 2 cut(s) 284, 745
MfeI CAATTG 1 cut(s) 564
MhlI GDGCHC 1 cut(s) 28
MluCI AATT 8 cut(s) 189, 323, 355, 381, 474, 496, 564, 608
MnlI CCTC 9 cut(s) 107, 281, 360, 442, 480, 586, 594, 655, 733
MseI TTAA 2 cut(s) 87, 501
MspI CCGG 1 cut(s) 209
MspR9I CCNGG 2 cut(s) 21, 761
MunI CAATTG 1 cut(s) 564
MvaI CCWGG 2 cut(s) 21, 761
MwoI GCNNNNNNNGC 5 cut(s) 410, 569, 593, 602, 677
NcoI CCATGG 1 cut(s) 194
NlaIII CATG 4 cut(s) 198, 376, 392, 787
NmuCI GTSAC 1 cut(s) 266
PfeI GAWTC 1 cut(s) 773
PfoI TCCNGGA 1 cut(s) 19
PkrI GCNGC 3 cut(s) 493, 586, 745
PsiI TTATAA 1 cut(s) 188
Psp124BI GAGCTC 1 cut(s) 28
Psp6I CCWGG 2 cut(s) 19, 759
PspGI CCWGG 2 cut(s) 19, 759
PspPI GGNCC 1 cut(s) 123
SacI GAGCTC 1 cut(s) 28
SaqAI TTAA 2 cut(s) 87, 501
SatI GCNGC 3 cut(s) 492, 585, 744
Sau96I GGNCC 1 cut(s) 123
ScrFI CCNGG 2 cut(s) 21, 761
SduI GDGCHC 1 cut(s) 28
SfcI CTRYAG 2 cut(s) 330, 405
SinI GGWCC 1 cut(s) 123
Sse9I AATT 8 cut(s) 189, 323, 355, 381, 474, 496, 564, 608
SstI GAGCTC 1 cut(s) 28
StyD4I CCNGG 2 cut(s) 19, 759
StyI CCWWGG 1 cut(s) 194
TaaI ACNGT 3 cut(s) 52, 334, 417
TaqI TCGA 3 cut(s) 108, 295, 340
TasI AATT 8 cut(s) 189, 323, 355, 381, 474, 496, 564, 608
TfiI GAWTC 1 cut(s) 773
Tru1I TTAA 2 cut(s) 87, 501
Tru9I TTAA 2 cut(s) 87, 501
TscAI CASTG 1 cut(s) 516
TseFI GTSAC 1 cut(s) 266
TseI GCWGC 3 cut(s) 491, 584, 743
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 3 cut(s) 17, 405, 819
TspGWI ACGGA 2 cut(s) 484, 745
TspRI CASTG 1 cut(s) 516
VpaK11BI GGWCC 1 cut(s) 123
XapI RAATTY 3 cut(s) 474, 496, 608
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.