MD03G1195500.v1.1

Prohibitin-1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001997
Physical Location & Seq
Reverse (-)
26801618 .. 26805419
3802 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1195500.v1.1.491

Sequence Viewer

Length: 957 bp
ATGAAAGTTCCCAAACTGCCTGGAGCTCGTGGCATTGCTACTTTGCTTACGGTGGGAGTTGTTGGTGGTCTTGGTTTATATGGACTTAACAATAGTCTCTACAATGTCATGGAAGGCATAGGGTGTGAGTTCGAAGGTTCTTCCATTGTGCAAGGAAGAACTTTATCAGAAGAAGAAGGTTCTGGGCTGTTTTTTCCTGGGGACGACGTGGTGTTTGTGAACTGCTTGCACACTTTGGGTAAAGCCGCGAAACGTCTTAAAGAGAGCGACTTAGTCCGCAACTCATCCCAAGAATCATTCACCACTCTAGGCTTCTACATTTTTCGGGTAACTTCGTTCATTTTTTATTTCAGTTTACAACACCTAATAATCCCATGGTATGAAAGGCCAGTCATCTATGATGTTCGTGCAAAACCTTATGTAGTGGAGAGTAAAGCTGGAAGCCGTGACCTCCAGATGGTGTTAGTAGAGCTTCGAGTGCTGACCCGTCCCGTATCAGACGAATTACCTACAGTTTATCGAACTCTGGTTGCACAGTATAATGCCAGCCAACTTATTACTCAGAAAGAGAGAAGGGAGATGCGGAAAATTTTGACAGAGAAGGCAGCGAATTTTAACATTGCACTGGATGATGTGTCTATCACTAACCTGACTTTCGGAAAGGAGTTTCTGAATGCAATTGAAGCGAAACAGGTAGCTGCACAAGAGGCTGAGAGGGTAAAATTCGTTGTGGAGAAAGCCGAACAAGACAAGAAAAGTGCTGTCATCAAAGCAGAGGGCGAAGCCACGAGTGCAAAGCTGATCAGTCAAGTCATCGCCAACAATCCAGCGTTTATCACTCTAAGGAAGATCGAGGCTGCAAGAGAAATCGCACGAGTTATCTCAGATTCCAAAAACAAGATTTACTTGAACGCCGATGAACTTTTGCTGAACATTCAGGAGACGAATGTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Band_7

Protein Analysis

319

Amino Acids

35.42

Weight (kDa)

7.67

Isoelectric Point (pI)

36.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Band_7 PF01145 174 - 250 1.7e-07 SPFH domain / Band 7 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 248
AciI CCGC 3 cut(s) 246, 277, 583
AcsI RAATTY 3 cut(s) 588, 610, 722
AfaI GTAC 1 cut(s) 951
AfiI CCNNNNNNNGG 1 cut(s) 457
AgsI TTSAA 2 cut(s) 683, 910
AjiI CACGTC 1 cut(s) 208
AjnI CCWGG 2 cut(s) 19, 196
AluBI AGCT 5 cut(s) 26, 437, 472, 698, 799
AluI AGCT 5 cut(s) 26, 437, 472, 698, 799
Alw21I GWGCWC 1 cut(s) 28
Alw26I GTCTC 2 cut(s) 101, 935
AoxI GGCC 1 cut(s) 386
ApeKI GCWGC 3 cut(s) 605, 698, 857
ApoI RAATTY 3 cut(s) 588, 610, 722
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AsuHPI GGTGA 1 cut(s) 292
AsuII TTCGAA 1 cut(s) 132
BanII GRGCYC 1 cut(s) 28
BauI CACGAG 3 cut(s) 27, 787, 873
Bbv12I GWGCWC 1 cut(s) 28
BbvI GCAGC 3 cut(s) 617, 685, 844
BccI CCATC 1 cut(s) 451
BceAI ACGGC 1 cut(s) 429
BciT130I CCWGG 2 cut(s) 21, 198
BclI TGATCA 1 cut(s) 801
BcoDI GTCTC 2 cut(s) 101, 935
BfaI CTAG 1 cut(s) 308
BfmI CTRYAG 1 cut(s) 510
BisI GCNGC 4 cut(s) 246, 606, 699, 858
BlsI GCNGC 4 cut(s) 247, 607, 700, 859
Bme1390I CCNGG 2 cut(s) 21, 198
BmgBI CACGTC 1 cut(s) 208
BmrFI CCNGG 2 cut(s) 21, 198
BmsI GCATC 1 cut(s) 570
BpmI CTGGAG 2 cut(s) 42, 437
Bpu14I TTCGAA 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 197, 374
Bsc4I CCNNNNNNNGG 1 cut(s) 457
Bse1I ACTGG 2 cut(s) 389, 630
Bse3DI GCAATG 2 cut(s) 33, 618
BseBI CCWGG 2 cut(s) 21, 198
BseDI CCNNGG 2 cut(s) 197, 374
BseGI GGATG 2 cut(s) 284, 634
BseLI CCNNNNNNNGG 1 cut(s) 457
BseMI GCAATG 2 cut(s) 33, 618
BseMII CTCAG 3 cut(s) 575, 702, 897
BseNI ACTGG 2 cut(s) 389, 630
BseXI GCAGC 3 cut(s) 617, 685, 844
BsgI GTGCAG 1 cut(s) 684
Bsh1236I CGCG 1 cut(s) 248
BshFI GGCC 1 cut(s) 388
BsiHKAI GWGCWC 1 cut(s) 28
BslFI GGGAC 2 cut(s) 215, 474
BslI CCNNNNNNNGG 1 cut(s) 457
BsmAI GTCTC 2 cut(s) 101, 935
BsmBI CGTCTC 1 cut(s) 935
BsmFI GGGAC 2 cut(s) 215, 474
BsmI GAATGC 1 cut(s) 679
BsnI GGCC 1 cut(s) 388
Bsp119I TTCGAA 1 cut(s) 132
Bsp1286I GDGCHC 1 cut(s) 28
Bsp1407I TGTACA 1 cut(s) 949
Bsp143I GATC 2 cut(s) 801, 849
Bsp19I CCATGG 1 cut(s) 374
BspACI CCGC 3 cut(s) 246, 277, 583
BspANI GGCC 1 cut(s) 388
BspCNI CTCAG 3 cut(s) 574, 703, 896
BspFNI CGCG 1 cut(s) 248
BspT104I TTCGAA 1 cut(s) 132
BsrDI GCAATG 2 cut(s) 33, 618
BsrGI TGTACA 1 cut(s) 949
BsrI ACTGG 2 cut(s) 389, 630
BssECI CCNNGG 2 cut(s) 197, 374
BssMI GATC 2 cut(s) 801, 849
BssSI CACGAG 3 cut(s) 27, 787, 873
BssT1I CCWWGG 1 cut(s) 374
Bst2BI CACGAG 3 cut(s) 27, 787, 873
Bst2UI CCWGG 2 cut(s) 21, 198
Bst4CI ACNGT 4 cut(s) 52, 514, 537, 954
BstAUI TGTACA 1 cut(s) 949
BstBI TTCGAA 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 227, 547
BstDEI CTNAG 5 cut(s) 271, 561, 711, 842, 883
BstDSI CCRYGG 1 cut(s) 374
BstF5I GGATG 2 cut(s) 284, 634
BstFNI CGCG 1 cut(s) 248
BstKTI GATC 2 cut(s) 804, 852
BstMAI GTCTC 2 cut(s) 101, 935
BstMBI GATC 2 cut(s) 801, 849
BstMWI GCNNNNNNNGC 4 cut(s) 478, 683, 707, 791
BstNI CCWGG 2 cut(s) 21, 198
BstSCI CCNGG 2 cut(s) 19, 196
BstSFI CTRYAG 1 cut(s) 510
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 3 cut(s) 617, 685, 844
BsuRI GGCC 1 cut(s) 388
BtgI CCRYGG 1 cut(s) 374
BtgZI GCGATG 1 cut(s) 799
BtrI CACGTC 1 cut(s) 208
BtsCI GGATG 2 cut(s) 284, 634
BtsIMutI CAGTG 1 cut(s) 623
Cac8I GCNNGC 2 cut(s) 227, 547
Csp6I GTAC 1 cut(s) 950
CviAII CATG 2 cut(s) 109, 375
CviQI GTAC 1 cut(s) 950
DdeI CTNAG 5 cut(s) 271, 561, 711, 842, 883
DpnI GATC 2 cut(s) 803, 851
DpnII GATC 2 cut(s) 801, 849
Ecl136II GAGCTC 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 374
Eco24I GRGCYC 1 cut(s) 28
Eco53kI GAGCTC 1 cut(s) 26
EcoICRI GAGCTC 1 cut(s) 26
EcoRII CCWGG 2 cut(s) 19, 196
EcoT14I CCWWGG 1 cut(s) 374
EcoT38I GRGCYC 1 cut(s) 28
ErhI CCWWGG 1 cut(s) 374
Esp3I CGTCTC 1 cut(s) 935
FaeI CATG 2 cut(s) 112, 378
FaiI YATR 9 cut(s) 79, 81, 110, 119, 376, 381, 399, 420, 540
FalI AAGNNNNNCTT 2 cut(s) 890, 922
FaqI GGGAC 2 cut(s) 215, 474
FatI CATG 2 cut(s) 108, 374
FbaI TGATCA 1 cut(s) 801
Fnu4HI GCNGC 4 cut(s) 246, 606, 699, 858
FokI GGATG 2 cut(s) 271, 641
FriOI GRGCYC 1 cut(s) 28
Fsp4HI GCNGC 4 cut(s) 246, 606, 699, 858
FspBI CTAG 1 cut(s) 308
GluI GCNGC 4 cut(s) 246, 606, 699, 858
GsuI CTGGAG 2 cut(s) 42, 437
HaeIII GGCC 1 cut(s) 388
Hin1II CATG 2 cut(s) 112, 378
HinfI GANTC 2 cut(s) 293, 887
HphI GGTGA 1 cut(s) 292
Hpy166II GTNNAC 2 cut(s) 220, 356
Hpy188I TCNGA 6 cut(s) 169, 499, 564, 659, 672, 886
Hpy188III TCNNGA 2 cut(s) 454, 938
Hpy8I GTNNAC 2 cut(s) 220, 356
Hpy99I CGWCG 1 cut(s) 209
HpyAV CCTTC 5 cut(s) 107, 128, 170, 567, 595
HpyCH4III ACNGT 4 cut(s) 52, 514, 537, 954
HpyCH4IV ACGT 2 cut(s) 207, 253
HpyCH4V TGCA 9 cut(s) 151, 229, 410, 533, 623, 677, 701, 794, 860
HpyF10VI GCNNNNNNNGC 4 cut(s) 478, 683, 707, 791
HpyF3I CTNAG 5 cut(s) 271, 561, 711, 842, 883
HpySE526I ACGT 2 cut(s) 207, 253
Hsp92II CATG 2 cut(s) 112, 378
Ksp22I TGATCA 1 cut(s) 801
Kzo9I GATC 2 cut(s) 801, 849
LmnI GCTCC 1 cut(s) 23
Lsp1109I GCAGC 3 cut(s) 617, 685, 844
LweI GCATC 1 cut(s) 570
MaeI CTAG 1 cut(s) 308
MaeII ACGT 2 cut(s) 207, 253
MaeIII GTNAC 2 cut(s) 328, 446
MalI GATC 2 cut(s) 803, 851
MboI GATC 2 cut(s) 801, 849
MboII GAAGA 5 cut(s) 132, 168, 182, 185, 859
MfeI CAATTG 1 cut(s) 678
MhlI GDGCHC 1 cut(s) 28
MluCI AATT 5 cut(s) 503, 588, 610, 678, 722
MnlI CCTC 5 cut(s) 461, 700, 708, 769, 847
MseI TTAA 3 cut(s) 87, 258, 615
MspR9I CCNGG 2 cut(s) 21, 198
MunI CAATTG 1 cut(s) 678
Mva1269I GAATGC 1 cut(s) 679
MvaI CCWGG 2 cut(s) 21, 198
MvnI CGCG 1 cut(s) 248
MwoI GCNNNNNNNGC 4 cut(s) 478, 683, 707, 791
NcoI CCATGG 1 cut(s) 374
NdeII GATC 2 cut(s) 801, 849
NlaIII CATG 2 cut(s) 112, 378
NmuCI GTSAC 1 cut(s) 446
NspV TTCGAA 1 cut(s) 132
PctI GAATGC 1 cut(s) 679
PfeI GAWTC 2 cut(s) 293, 887
PflFI GACNNNGTC 1 cut(s) 272
PkrI GCNGC 4 cut(s) 247, 607, 700, 859
Psp124BI GAGCTC 1 cut(s) 28
Psp6I CCWGG 2 cut(s) 19, 196
PspGI CCWGG 2 cut(s) 19, 196
PsyI GACNNNGTC 1 cut(s) 272
RsaI GTAC 1 cut(s) 951
RsaNI GTAC 1 cut(s) 950
SacI GAGCTC 1 cut(s) 28
SaqAI TTAA 3 cut(s) 87, 258, 615
SatI GCNGC 4 cut(s) 246, 606, 699, 858
Sau3AI GATC 2 cut(s) 801, 849
ScrFI CCNGG 2 cut(s) 21, 198
SduI GDGCHC 1 cut(s) 28
SfaNI GCATC 1 cut(s) 570
SfcI CTRYAG 1 cut(s) 510
SfuI TTCGAA 1 cut(s) 132
Sse9I AATT 5 cut(s) 503, 588, 610, 678, 722
SsiI CCGC 3 cut(s) 246, 277, 583
SspMI CTAG 1 cut(s) 308
SstI GAGCTC 1 cut(s) 28
StyD4I CCNGG 2 cut(s) 19, 196
StyI CCWWGG 1 cut(s) 374
TaaI ACNGT 4 cut(s) 52, 514, 537, 954
TaiI ACGT 2 cut(s) 210, 256
TaqI TCGA 4 cut(s) 132, 475, 520, 852
TasI AATT 5 cut(s) 503, 588, 610, 678, 722
TatI WGTACW 1 cut(s) 949
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 2 cut(s) 293, 887
Tru1I TTAA 3 cut(s) 87, 258, 615
Tru9I TTAA 3 cut(s) 87, 258, 615
TscAI CASTG 1 cut(s) 630
TseFI GTSAC 1 cut(s) 446
TseI GCWGC 3 cut(s) 605, 698, 857
Tsp45I GTSAC 1 cut(s) 446
TspDTI ATGAA 4 cut(s) 17, 328, 396, 933
TspRI CASTG 1 cut(s) 630
Tth111I GACNNNGTC 1 cut(s) 272
XapI RAATTY 3 cut(s) 588, 610, 722
XspI CTAG 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.