AT5G09580

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003644
Physical Location & Seq
Forward (+)
2972639 .. 2974776
2138 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G09580.2

Sequence Viewer

Length: 1182 bp
ATGCAATTAAAAGCCAATTTCCTCCGCTTCAAAATCTCAGTCGTGTGCGCACATAAACCTTTACCAAAATCCCAGCAATCGATGATTTTCCGGCGATTCTCCGGGAGATTTCTAACTGCGATCCCCGACTCTTGTAAACTTAAATTCGAAGCTAAACGGAGTTTCCGATCTGATGCGGCGCTCGAAGCCATAGCTAATGCATTAGAAGAGAAAGTACCAAACTTAGTGTTATACAATTATCCTTCCTTCTCCGGCGCTTTTTCAGCTCTCTTCGCTCATCTCTACCACTATCGACTCCGTCTTCCTTGTCTCATCTTACCCTTTTCTTCCCTCATACCCTTTAGGGTTGAAGATTTGTGTTTGGAGGGGTTTGAGAGGTGTTACCTTCTCGATTTTGTTGTTCCAAAAGATTTTGCTTGCCAGAAAACAGCTTGCGAGATCGTTTGCTTTGATCATCGGAACTCCGCATTGAAAAGGATTGGCTTAATAAAGGAAGAGCACAAGAAGAGGCTTAAGATTTATGTTGATACTGAAACTAGTAGCTCAAAGGCTGTGTATAAATATTTCTCTAGTAAACTCACAGATCAAAGATCTTCTGAGGTAGAAGCTCTCAGTTTGCTAAGTGTAGAAGACAAAACTCGGGTTGAATCGGTTCTTGATTACATTGAGGATATCGATCTTCGGCGATGGATGTTACCAGATATCAAAGCTTTCAGCTTTGGTCTTAAGGATTGGCGCTCAAGGATCAATTGTATCATCAATCCTTACATGTACGAACAGTTGTTGAAAATAAGTTCAGCGGATCTCATTGCGTATGGGAATTCGTATTTTTCATCTCGTCTTATTGATGCAAAGAAACTGCTAAAGCTGAATAAAGCTTTCAAGATTAGATTGGGAAGAGGGTTATATGGAGAGTGCCTGGGAATGCGCGCAGATGGTAACCATCAACTAAGCGATGAACTTGGCAAGCTTCTTAGTCTACAAAGTTCTGCAGCTGGTCTGAGGCCTATAGGAGCTGTTACATTTGTGCAAAGGAATAATCTCAAAATGTGCTTGAGAAGTACCGATGCTATAACCAATACATCCGAAGTTGCTAAGGCTTATGGAGGAGGTGGAACGTCTAGTTCAAGCTCGTTTATCATAAGAATGGATGAATATAATCAATGGATTTCGAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

393

Amino Acids

44.73

Weight (kDa)

9.34

Isoelectric Point (pI)

49.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 49
AccI GTMKAC 1 cut(s) 979
AccII CGCG 1 cut(s) 930
AciI CCGC 4 cut(s) 25, 176, 465, 800
AclWI GGATC 3 cut(s) 115, 752, 810
AcsI RAATTY 2 cut(s) 143, 820
AfaI GTAC 3 cut(s) 216, 773, 1063
AflII CTTAAG 2 cut(s) 512, 725
AflIII ACRYGT 1 cut(s) 768
AgsI TTSAA 7 cut(s) 31, 350, 472, 647, 787, 883, 1128
AhlI ACTAGT 1 cut(s) 536
AjnI CCWGG 1 cut(s) 918
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 1 cut(s) 314
AlwI GGATC 3 cut(s) 115, 752, 810
Ama87I CYCGRG 1 cut(s) 639
AoxI GGCC 1 cut(s) 1004
ApeKI GCWGC 1 cut(s) 992
ApoI RAATTY 2 cut(s) 143, 820
ArsI GACNNNNNNTTYG 2 cut(s) 24, 56
Asp700I GAANNNNTTC 1 cut(s) 651
AspLEI GCGC 6 cut(s) 50, 181, 257, 738, 930, 932
AsuC2I CCSGG 1 cut(s) 103
AsuII TTCGAA 2 cut(s) 147, 1172
AvaI CYCGRG 1 cut(s) 639
BarI GAAGNNNNNNTAC 2 cut(s) 198, 230
BbsI GAAGAC 2 cut(s) 293, 636
Bbv12I GWGCWC 1 cut(s) 501
BbvI GCAGC 1 cut(s) 1004
BccI CCATC 3 cut(s) 681, 929, 951
BciT130I CCWGG 1 cut(s) 920
BclI TGATCA 1 cut(s) 451
BcnI CCSGG 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 314
BcuI ACTAGT 1 cut(s) 536
BfaI CTAG 3 cut(s) 537, 570, 1122
BfmI CTRYAG 2 cut(s) 990, 1008
BfoI RGCGCY 3 cut(s) 182, 258, 739
BfrI CTTAAG 2 cut(s) 512, 725
BglII AGATCT 1 cut(s) 590
BisI GCNGC 2 cut(s) 177, 993
BlsI GCNGC 2 cut(s) 178, 994
Bme1390I CCNGG 2 cut(s) 103, 920
BmeT110I CYCGRG 1 cut(s) 639
BmrFI CCNGG 2 cut(s) 103, 920
BmsI GCATC 3 cut(s) 163, 838, 1057
BpiI GAAGAC 2 cut(s) 293, 636
Bpu10I CCTNAGC 1 cut(s) 1095
Bpu14I TTCGAA 2 cut(s) 147, 1172
BpuEI CTTGAG 2 cut(s) 724, 1075
BpuMI CCSGG 1 cut(s) 103
Bsa29I ATCGAT 2 cut(s) 80, 675
BsaBI GATNNNNATC 1 cut(s) 675
BsaJI CCNNGG 1 cut(s) 919
Bse3DI GCAATG 1 cut(s) 807
Bse8I GATNNNNATC 1 cut(s) 675
BseBI CCWGG 1 cut(s) 920
BseCI ATCGAT 2 cut(s) 80, 675
BseDI CCNNGG 1 cut(s) 919
BseGI GGATG 3 cut(s) 696, 1082, 1156
BseJI GATNNNNATC 1 cut(s) 675
BseMI GCAATG 1 cut(s) 807
BseMII CTCAG 4 cut(s) 51, 588, 625, 992
BsePI GCGCGC 1 cut(s) 928
BseRI GAGGAG 1 cut(s) 1122
BseXI GCAGC 1 cut(s) 1004
BseYI CCCAGC 1 cut(s) 72
Bsh1236I CGCG 1 cut(s) 930
BshFI GGCC 1 cut(s) 1006
BshVI ATCGAT 2 cut(s) 80, 675
BsiHKAI GWGCWC 1 cut(s) 501
BsiHKCI CYCGRG 1 cut(s) 639
BsiSI CCGG 3 cut(s) 91, 102, 252
BsmAI GTCTC 1 cut(s) 314
BsmI GAATGC 1 cut(s) 930
BsnI GGCC 1 cut(s) 1006
BsoBI CYCGRG 1 cut(s) 639
Bsp119I TTCGAA 2 cut(s) 147, 1172
Bsp1286I GDGCHC 1 cut(s) 501
Bsp143I GATC 9 cut(s) 120, 167, 438, 451, 583, 590, 676, 744, 802
BspACI CCGC 4 cut(s) 25, 176, 465, 800
BspANI GGCC 1 cut(s) 1006
BspCNI CTCAG 4 cut(s) 50, 589, 624, 993
BspDI ATCGAT 2 cut(s) 80, 675
BspFNI CGCG 1 cut(s) 930
BspMAI CTGCAG 1 cut(s) 994
BspPI GGATC 3 cut(s) 115, 752, 810
BspQI GCTCTTC 1 cut(s) 489
BspT104I TTCGAA 2 cut(s) 147, 1172
BspTI CTTAAG 2 cut(s) 512, 725
BsrDI GCAATG 1 cut(s) 807
BssECI CCNNGG 1 cut(s) 919
BssHII GCGCGC 1 cut(s) 928
BssMI GATC 9 cut(s) 120, 167, 438, 451, 583, 590, 676, 744, 802
Bst2UI CCWGG 1 cut(s) 920
Bst4CI ACNGT 1 cut(s) 780
Bst6I CTCTTC 5 cut(s) 201, 275, 489, 500, 892
BstAFI CTTAAG 2 cut(s) 512, 725
BstBI TTCGAA 2 cut(s) 147, 1172
BstC8I GCNNGC 4 cut(s) 418, 433, 930, 968
BstDEI CTNAG 9 cut(s) 37, 223, 597, 611, 620, 950, 974, 1001, 1095
BstEII GGTNACC 1 cut(s) 938
BstF5I GGATG 3 cut(s) 696, 1082, 1156
BstFNI CGCG 1 cut(s) 930
BstH2I RGCGCY 3 cut(s) 182, 258, 739
BstHHI GCGC 6 cut(s) 50, 181, 257, 738, 930, 932
BstKTI GATC 9 cut(s) 123, 170, 441, 454, 586, 593, 679, 747, 805
BstMAI GTCTC 1 cut(s) 314
BstMBI GATC 9 cut(s) 120, 167, 438, 451, 583, 590, 676, 744, 802
BstMWI GCNNNNNNNGC 3 cut(s) 185, 263, 272
BstNI CCWGG 1 cut(s) 920
BstNSI RCATGY 1 cut(s) 772
BstPI GGTNACC 1 cut(s) 938
BstSCI CCNGG 2 cut(s) 101, 918
BstSFI CTRYAG 2 cut(s) 990, 1008
BstUI CGCG 1 cut(s) 930
BstV1I GCAGC 1 cut(s) 1004
BstV2I GAAGAC 2 cut(s) 293, 636
BstX2I RGATCY 2 cut(s) 590, 802
BstYI RGATCY 2 cut(s) 590, 802
Bsu15I ATCGAT 2 cut(s) 80, 675
BsuRI GGCC 1 cut(s) 1006
BsuTUI ATCGAT 2 cut(s) 80, 675
BtgZI GCGATG 2 cut(s) 700, 969
BtsCI GGATG 3 cut(s) 696, 1082, 1156
Cac8I GCNNGC 4 cut(s) 418, 433, 930, 968
CfoI GCGC 6 cut(s) 50, 181, 257, 738, 930, 932
ClaI ATCGAT 2 cut(s) 80, 675
Csp6I GTAC 3 cut(s) 215, 772, 1062
CviAII CATG 1 cut(s) 769
CviQI GTAC 3 cut(s) 215, 772, 1062
DdeI CTNAG 9 cut(s) 37, 223, 597, 611, 620, 950, 974, 1001, 1095
DpnI GATC 9 cut(s) 122, 169, 440, 453, 585, 592, 678, 746, 804
DpnII GATC 9 cut(s) 120, 167, 438, 451, 583, 590, 676, 744, 802
Eam1104I CTCTTC 5 cut(s) 201, 275, 489, 500, 892
EarI CTCTTC 5 cut(s) 201, 275, 489, 500, 892
Eco147I AGGCCT 1 cut(s) 1006
Eco32I GATATC 2 cut(s) 673, 703
Eco88I CYCGRG 1 cut(s) 639
Eco91I GGTNACC 1 cut(s) 938
EcoO65I GGTNACC 1 cut(s) 938
EcoRI GAATTC 1 cut(s) 820
EcoRII CCWGG 1 cut(s) 918
EcoRV GATATC 2 cut(s) 673, 703
EcoT22I ATGCAT 1 cut(s) 202
FaeI CATG 1 cut(s) 772
FatI CATG 1 cut(s) 768
FbaI TGATCA 1 cut(s) 451
FblI GTMKAC 1 cut(s) 979
Fnu4HI GCNGC 2 cut(s) 177, 993
FokI GGATG 3 cut(s) 703, 1069, 1163
Fsp4HI GCNGC 2 cut(s) 177, 993
FspAI RTGCGCAY 1 cut(s) 49
FspBI CTAG 3 cut(s) 537, 570, 1122
FspI TGCGCA 1 cut(s) 49
GlaI GCGC 6 cut(s) 49, 180, 256, 737, 929, 931
GluI GCNGC 2 cut(s) 177, 993
GsaI CCCAGC 1 cut(s) 76
HaeII RGCGCY 3 cut(s) 182, 258, 739
HaeIII GGCC 1 cut(s) 1006
HapII CCGG 3 cut(s) 91, 102, 252
HhaI GCGC 6 cut(s) 50, 181, 257, 738, 930, 932
Hin1II CATG 1 cut(s) 772
Hin6I GCGC 6 cut(s) 48, 179, 255, 736, 928, 930
HinP1I GCGC 6 cut(s) 48, 179, 255, 736, 928, 930
HindIII AAGCTT 4 cut(s) 708, 876, 968, 1175
HinfI GANTC 4 cut(s) 96, 128, 294, 647
HpaII CCGG 3 cut(s) 91, 102, 252
Hpy166II GTNNAC 3 cut(s) 137, 575, 980
Hpy188I TCNGA 6 cut(s) 167, 172, 459, 598, 1002, 1087
Hpy188III TCNNGA 3 cut(s) 389, 656, 883
Hpy8I GTNNAC 3 cut(s) 137, 575, 980
HpyAV CCTTC 3 cut(s) 252, 256, 395
HpyCH4III ACNGT 1 cut(s) 780
HpyCH4IV ACGT 1 cut(s) 1118
HpyCH4V TGCA 5 cut(s) 4, 200, 851, 992, 1030
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 263, 272
HpyF3I CTNAG 9 cut(s) 37, 223, 597, 611, 620, 950, 974, 1001, 1095
HpySE526I ACGT 1 cut(s) 1118
Hsp92II CATG 1 cut(s) 772
HspAI GCGC 6 cut(s) 48, 179, 255, 736, 928, 930
Ksp22I TGATCA 1 cut(s) 451
Kzo9I GATC 9 cut(s) 120, 167, 438, 451, 583, 590, 676, 744, 802
LguI GCTCTTC 1 cut(s) 489
LmnI GCTCC 1 cut(s) 1013
LpnPI CCDG 9 cut(s) 86, 104, 115, 265, 434, 711, 905, 932, 981
Lsp1109I GCAGC 1 cut(s) 1004
LweI GCATC 3 cut(s) 163, 838, 1057
MaeI CTAG 3 cut(s) 537, 570, 1122
MaeII ACGT 1 cut(s) 1118
MaeIII GTNAC 4 cut(s) 380, 693, 938, 1018
MalI GATC 9 cut(s) 122, 169, 440, 453, 585, 592, 678, 746, 804
MboI GATC 9 cut(s) 120, 167, 438, 451, 583, 590, 676, 744, 802
MfeI CAATTG 1 cut(s) 748
MflI RGATCY 2 cut(s) 590, 802
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 6 cut(s) 5, 16, 143, 235, 748, 820
MlyI GAGTC 2 cut(s) 122, 288
Mph1103I ATGCAT 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 651
MseI TTAA 5 cut(s) 8, 141, 485, 513, 726
MslI CAYNNNNRTG 1 cut(s) 1145
MspA1I CMGCKG 2 cut(s) 800, 995
MspCI CTTAAG 2 cut(s) 512, 725
MspI CCGG 3 cut(s) 91, 102, 252
MspR9I CCNGG 2 cut(s) 103, 920
MunI CAATTG 1 cut(s) 748
Mva1269I GAATGC 1 cut(s) 930
MvaI CCWGG 1 cut(s) 920
MvnI CGCG 1 cut(s) 930
MwoI GCNNNNNNNGC 3 cut(s) 185, 263, 272
NciI CCSGG 1 cut(s) 103
NdeII GATC 9 cut(s) 120, 167, 438, 451, 583, 590, 676, 744, 802
NlaIII CATG 1 cut(s) 772
NsbI TGCGCA 1 cut(s) 49
NsiI ATGCAT 1 cut(s) 202
NspI RCATGY 1 cut(s) 772
NspV TTCGAA 2 cut(s) 147, 1172
PauI GCGCGC 1 cut(s) 928
PceI AGGCCT 1 cut(s) 1006
PciI ACATGT 1 cut(s) 768
PciSI GCTCTTC 1 cut(s) 489
PcsI WCGNNNNNNNCGW 1 cut(s) 163
PctI GAATGC 1 cut(s) 930
PdmI GAANNNNTTC 1 cut(s) 651
PfeI GAWTC 2 cut(s) 96, 647
PflFI GACNNNGTC 1 cut(s) 297
PfoI TCCNGGA 1 cut(s) 101
PkrI GCNGC 2 cut(s) 178, 994
PleI GAGTC 2 cut(s) 122, 288
PpsI GAGTC 2 cut(s) 122, 288
PscI ACATGT 1 cut(s) 768
Psp6I CCWGG 1 cut(s) 918
PspEI GGTNACC 1 cut(s) 938
PspFI CCCAGC 1 cut(s) 72
PspGI CCWGG 1 cut(s) 918
PstI CTGCAG 1 cut(s) 994
PsuI RGATCY 2 cut(s) 590, 802
PsyI GACNNNGTC 1 cut(s) 297
PteI GCGCGC 1 cut(s) 928
PvuII CAGCTG 1 cut(s) 995
RsaI GTAC 3 cut(s) 216, 773, 1063
RsaNI GTAC 3 cut(s) 215, 772, 1062
RseI CAYNNNNRTG 1 cut(s) 1145
SapI GCTCTTC 1 cut(s) 489
SaqAI TTAA 5 cut(s) 8, 141, 485, 513, 726
SatI GCNGC 2 cut(s) 177, 993
Sau3AI GATC 9 cut(s) 120, 167, 438, 451, 583, 590, 676, 744, 802
SchI GAGTC 2 cut(s) 122, 288
ScrFI CCNGG 2 cut(s) 103, 920
SduI GDGCHC 1 cut(s) 501
SfaNI GCATC 3 cut(s) 163, 838, 1057
SfcI CTRYAG 2 cut(s) 990, 1008
SfuI TTCGAA 2 cut(s) 147, 1172
SmiMI CAYNNNNRTG 1 cut(s) 1145
SmlI CTYRAG 4 cut(s) 512, 725, 739, 1054
SmoI CTYRAG 4 cut(s) 512, 725, 739, 1054
SpeI ACTAGT 1 cut(s) 536
Sse9I AATT 6 cut(s) 5, 16, 143, 235, 748, 820
SseBI AGGCCT 1 cut(s) 1006
SsiI CCGC 4 cut(s) 25, 176, 465, 800
SspI AATATT 1 cut(s) 563
SspMI CTAG 3 cut(s) 537, 570, 1122
StuI AGGCCT 1 cut(s) 1006
StyD4I CCNGG 2 cut(s) 101, 918
TaaI ACNGT 1 cut(s) 780
TaiI ACGT 1 cut(s) 1121
TaqI TCGA 7 cut(s) 80, 147, 183, 292, 390, 675, 1172
TasI AATT 6 cut(s) 5, 16, 143, 235, 748, 820
TauI GCSGC 1 cut(s) 179
TfiI GAWTC 2 cut(s) 96, 647
Tru1I TTAA 5 cut(s) 8, 141, 485, 513, 726
Tru9I TTAA 5 cut(s) 8, 141, 485, 513, 726
TseI GCWGC 1 cut(s) 992
TspDTI ATGAA 3 cut(s) 822, 972, 1167
TspGWI ACGGA 2 cut(s) 172, 287
Tth111I GACNNNGTC 1 cut(s) 297
Vha464I CTTAAG 2 cut(s) 512, 725
XapI RAATTY 2 cut(s) 143, 820
XceI RCATGY 1 cut(s) 772
XmiI GTMKAC 1 cut(s) 979
XmnI GAANNNNTTC 1 cut(s) 651
XspI CTAG 3 cut(s) 537, 570, 1122
Zsp2I ATGCAT 1 cut(s) 202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.