pycom10g04330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0003644
Physical Location & Seq
Forward (+)
4578350 .. 4579007
658 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g04330.3

Sequence Viewer

Length: 411 bp
ATGCCTCATTTCCTTGTCGGAAAATGGAGAACCACCATCTTTGCCTCGGCTTCTCCGCTGTCACAAGCCCGGAGCTTCCGTTCAGACGCAGCGTTAGAAGCCCTGGCCAAAGCCTCTCAAGACAAAGTTCCCAACTTGCTTCTCTACAACTACCCTTCTTTCTCTGGAGCTTTCTCCGCTCTATTCGCTTATCTCTTTCACTCTCGCCTCAACCTCCCCTTTCTCTCTCTTCCTTTCTCTTCCGTCGAACCCTTCAGAGTTGATGAGTTGCGGACTGAAGGGCTGGAAAGGTGCTATCTTGTTGATTTTCTTGGCCGGGAGGGATTTGCGGTCGAGCTTGCGCAGAGAGCTTCGTGCGAGGTAATTGCTTTTGATCGATCGAAAAAGGGTACTTCCACAGATACCCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

137

Amino Acids

15.19

Weight (kDa)

7.79

Isoelectric Point (pI)

41.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 342
AccBSI CCGCTC 1 cut(s) 179
AciI CCGC 4 cut(s) 56, 177, 271, 329
AcoI YGGCCR 2 cut(s) 105, 313
AcuI CTGAAG 2 cut(s) 238, 297
AfaI GTAC 1 cut(s) 391
AjnI CCWGG 1 cut(s) 102
AloI GAACNNNNNNTCC 2 cut(s) 64, 96
AluBI AGCT 4 cut(s) 75, 170, 337, 350
AluI AGCT 4 cut(s) 75, 170, 337, 350
AoxI GGCC 2 cut(s) 105, 313
ApeKI GCWGC 1 cut(s) 89
AspLEI GCGC 1 cut(s) 343
AsuC2I CCSGG 2 cut(s) 70, 317
BalI TGGCCA 1 cut(s) 107
BbvI GCAGC 1 cut(s) 101
BccI CCATC 1 cut(s) 44
BcgI CGANNNNNNTGC 2 cut(s) 347, 381
BciT130I CCWGG 1 cut(s) 104
BcnI CCSGG 2 cut(s) 70, 317
BisI GCNGC 1 cut(s) 90
BlsI GCNGC 1 cut(s) 91
Bme1390I CCNGG 3 cut(s) 70, 104, 317
BmrFI CCNGG 3 cut(s) 70, 104, 317
BpmI CTGGAG 1 cut(s) 186
BpuEI CTTGAG 1 cut(s) 102
BpuMI CCSGG 2 cut(s) 70, 317
Bsa29I ATCGAT 1 cut(s) 376
BsaJI CCNNGG 2 cut(s) 45, 102
BsaXI ACNNNNNCTCC 2 cut(s) 64, 94
BseBI CCWGG 1 cut(s) 104
BseCI ATCGAT 1 cut(s) 376
BseDI CCNNGG 2 cut(s) 45, 102
BseXI GCAGC 1 cut(s) 101
Bsh1285I CGRYCG 2 cut(s) 333, 380
BshFI GGCC 2 cut(s) 107, 315
BshVI ATCGAT 1 cut(s) 376
BsiEI CGRYCG 2 cut(s) 333, 380
BsiSI CCGG 2 cut(s) 70, 316
BsnI GGCC 2 cut(s) 107, 315
Bsp143I GATC 2 cut(s) 373, 377
BspACI CCGC 4 cut(s) 56, 177, 271, 329
BspANI GGCC 2 cut(s) 107, 315
BspDI ATCGAT 1 cut(s) 376
BsrBI CCGCTC 1 cut(s) 179
BssECI CCNNGG 2 cut(s) 45, 102
BssMI GATC 2 cut(s) 373, 377
Bst2UI CCWGG 1 cut(s) 104
Bst6I CTCTTC 2 cut(s) 234, 244
BstC8I GCNNGC 1 cut(s) 339
BstHHI GCGC 1 cut(s) 343
BstKTI GATC 2 cut(s) 376, 380
BstMBI GATC 2 cut(s) 373, 377
BstMCI CGRYCG 2 cut(s) 333, 380
BstMWI GCNNNNNNNGC 4 cut(s) 98, 176, 185, 347
BstNI CCWGG 1 cut(s) 104
BstSCI CCNGG 3 cut(s) 68, 102, 315
BstV1I GCAGC 1 cut(s) 101
Bsu15I ATCGAT 1 cut(s) 376
BsuRI GGCC 2 cut(s) 107, 315
BsuTUI ATCGAT 1 cut(s) 376
Cac8I GCNNGC 1 cut(s) 339
CfoI GCGC 1 cut(s) 343
ClaI ATCGAT 1 cut(s) 376
CseI GACGC 1 cut(s) 95
Csp6I GTAC 1 cut(s) 390
CspCI CAANNNNNGTGG 2 cut(s) 22, 57
CviQI GTAC 1 cut(s) 390
DpnI GATC 2 cut(s) 375, 379
DpnII GATC 2 cut(s) 373, 377
EaeI YGGCCR 2 cut(s) 105, 313
Eam1104I CTCTTC 2 cut(s) 234, 244
EarI CTCTTC 2 cut(s) 234, 244
Eco57I CTGAAG 2 cut(s) 238, 297
EcoRII CCWGG 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 90
Fsp4HI GCNGC 1 cut(s) 90
FspI TGCGCA 1 cut(s) 342
GlaI GCGC 1 cut(s) 342
GluI GCNGC 1 cut(s) 90
GsuI CTGGAG 1 cut(s) 186
HaeIII GGCC 2 cut(s) 107, 315
HapII CCGG 2 cut(s) 70, 316
HgaI GACGC 1 cut(s) 95
HhaI GCGC 1 cut(s) 343
Hin6I GCGC 1 cut(s) 341
HinP1I GCGC 1 cut(s) 341
HpaII CCGG 2 cut(s) 70, 316
Hpy188I TCNGA 3 cut(s) 20, 85, 257
Hpy188III TCNNGA 2 cut(s) 119, 165
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 3 cut(s) 165, 262, 272
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 176, 185, 347
HspAI GCGC 1 cut(s) 341
Kzo9I GATC 2 cut(s) 373, 377
LmnI GCTCC 2 cut(s) 72, 167
LpnPI CCDG 6 cut(s) 83, 89, 116, 150, 269, 329
Lsp1109I GCAGC 1 cut(s) 101
MaeIII GTNAC 1 cut(s) 60
MalI GATC 2 cut(s) 375, 379
MbiI CCGCTC 1 cut(s) 179
MboI GATC 2 cut(s) 373, 377
MboII GAAGA 2 cut(s) 221, 231
MlsI TGGCCA 1 cut(s) 107
MluCI AATT 1 cut(s) 363
MluNI TGGCCA 1 cut(s) 107
MnlI CCTC 7 cut(s) 15, 55, 124, 218, 224, 313, 352
Mox20I TGGCCA 1 cut(s) 107
MscI TGGCCA 1 cut(s) 107
Msp20I TGGCCA 1 cut(s) 107
MspA1I CMGCKG 1 cut(s) 58
MspI CCGG 2 cut(s) 70, 316
MspR9I CCNGG 3 cut(s) 70, 104, 317
MvaI CCWGG 1 cut(s) 104
MwoI GCNNNNNNNGC 4 cut(s) 98, 176, 185, 347
NciI CCSGG 2 cut(s) 70, 317
NdeII GATC 2 cut(s) 373, 377
NmeAIII GCCGAG 1 cut(s) 26
NmuCI GTSAC 1 cut(s) 60
NsbI TGCGCA 1 cut(s) 342
PkrI GCNGC 1 cut(s) 91
Ple19I CGATCG 1 cut(s) 380
Psp6I CCWGG 1 cut(s) 102
PspGI CCWGG 1 cut(s) 102
PvuI CGATCG 1 cut(s) 380
RsaI GTAC 1 cut(s) 391
RsaNI GTAC 1 cut(s) 390
SatI GCNGC 1 cut(s) 90
Sau3AI GATC 2 cut(s) 373, 377
ScrFI CCNGG 3 cut(s) 70, 104, 317
SetI ASST 7 cut(s) 77, 172, 216, 293, 339, 352, 363
SmlI CTYRAG 1 cut(s) 117
SmoI CTYRAG 1 cut(s) 117
Sse9I AATT 1 cut(s) 363
SsiI CCGC 4 cut(s) 56, 177, 271, 329
StyD4I CCNGG 3 cut(s) 68, 102, 315
TaqI TCGA 4 cut(s) 246, 333, 376, 380
TasI AATT 1 cut(s) 363
TseFI GTSAC 1 cut(s) 60
TseI GCWGC 1 cut(s) 89
Tsp45I GTSAC 1 cut(s) 60
TspGWI ACGGA 2 cut(s) 68, 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.