AT5G14670

ARF-like small GTPases; ARF, ADP-ribosylation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000844
Physical Location & Seq
Forward (+)
4728947 .. 4730952
2006 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G14670.3

Sequence Viewer

Length: 567 bp
ATGGGGCTTAATTTCACAAAGTTGTTCAGCCGGCTTTTTGCCAAGAAAGAAATGAGGATTCTGATGGTAGGTCTTGATGCTGCTGGTAAGACCACTATCTTGTATAAGCTGAAGCTTGGCGAAATCGTCACTACCATCCCGACCATCGGATTCAACGTGGAGACTGTTGAATACAAGAACATCAGCTTCACTGTTTGGGATGTGGGGGGTCAGGACAAGATTCGTCCCTTGTGGAGGCACTATTTCCAAAACACTCAAGGTCTTATATTCGTGGTTGATAGCAACGACAGGGACCGAGTTGTGGAAGCGAGGGATGAGTTACATCGCATGTTGAATGAGGATGAACTGAGGGATGCTGTCCTTCTTGTGTTTGCAAACAAACAAGATCTTCCCAACGCAATGAATGCTGCAGAAATAACTGATAAGCTTGGTCTTCACTCTCTTCGCCAACGCCATTGGTACATTCAAAGCACTTGTGCCACATCTGGAGAAGGTCTCTATGAAGGCCTTGATTGGCTCTCCAACAACATCGCTACCAAGGTAAATAACTTAAATATCCCATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko05110 ko05134 map04072 map04144 map05110 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

188

Amino Acids

21.53

Weight (kDa)

6.43

Isoelectric Point (pI)

32.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 5 - 177 1.2e-81 ADP-ribosylation factor family
SRPRB PF09439 16 - 143 4.8e-13 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 129 8.3e-14 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 173 5.2e-12 Ras family
Gtr1_RagA PF04670 19 - 141 1.6e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 45 - 111 7.9e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcuI CTGAAG 1 cut(s) 131
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 3 cut(s) 146, 234, 301
AgsI TTSAA 4 cut(s) 154, 170, 334, 467
AluBI AGCT 4 cut(s) 109, 115, 186, 427
AluI AGCT 4 cut(s) 109, 115, 186, 427
Alw26I GTCTC 2 cut(s) 155, 500
AoxI GGCC 1 cut(s) 505
ApeKI GCWGC 2 cut(s) 80, 407
AspS9I GGNCC 1 cut(s) 292
AvaII GGWCC 1 cut(s) 292
BbsI GAAGAC 1 cut(s) 425
BbvI GCAGC 2 cut(s) 67, 394
BccI CCATC 3 cut(s) 58, 143, 152
BcoDI GTCTC 2 cut(s) 155, 500
BfmI CTRYAG 1 cut(s) 408
BglII AGATCT 1 cut(s) 385
BisI GCNGC 2 cut(s) 81, 408
BlsI GCNGC 2 cut(s) 82, 409
Bme18I GGWCC 1 cut(s) 292
BmgT120I GGNCC 1 cut(s) 292
BmiI GGNNCC 1 cut(s) 293
BmsI GCATC 2 cut(s) 67, 343
BpiI GAAGAC 1 cut(s) 425
BplI GAGNNNNNCTC 2 cut(s) 480, 512
BpmI CTGGAG 1 cut(s) 507
BpuEI CTTGAG 1 cut(s) 240
BsaI GGTCTC 1 cut(s) 500
BsaJI CCNNGG 1 cut(s) 537
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 234, 301
Bse118I RCCGGY 1 cut(s) 30
Bse3DI GCAATG 1 cut(s) 405
BseDI CCNNGG 1 cut(s) 537
BseGI GGATG 5 cut(s) 135, 205, 319, 346, 358
BseLI CCNNNNNNNGG 3 cut(s) 146, 234, 301
BseMI GCAATG 1 cut(s) 405
BseMII CTCAG 1 cut(s) 338
BseXI GCAGC 2 cut(s) 67, 394
BshFI GGCC 1 cut(s) 507
BsiSI CCGG 1 cut(s) 31
BslFI GGGAC 2 cut(s) 210, 305
BslI CCNNNNNNNGG 3 cut(s) 146, 234, 301
BsmAI GTCTC 2 cut(s) 155, 500
BsmFI GGGAC 2 cut(s) 210, 305
BsmI GAATGC 1 cut(s) 409
BsnI GGCC 1 cut(s) 507
Bso31I GGTCTC 1 cut(s) 500
Bsp143I GATC 1 cut(s) 385
BspANI GGCC 1 cut(s) 507
BspCNI CTCAG 1 cut(s) 339
BspLI GGNNCC 1 cut(s) 293
BspMAI CTGCAG 1 cut(s) 412
BspTNI GGTCTC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 405
BsrFI RCCGGY 1 cut(s) 30
BssAI RCCGGY 1 cut(s) 30
BssECI CCNNGG 1 cut(s) 537
BssMI GATC 1 cut(s) 385
BssT1I CCWWGG 1 cut(s) 537
Bst4CI ACNGT 2 cut(s) 166, 193
Bst6I CTCTTC 1 cut(s) 447
BstAPI GCANNNNNTGC 1 cut(s) 404
BstC8I GCNNGC 1 cut(s) 32
BstDEI CTNAG 1 cut(s) 347
BstENI CCTNNNNNAGG 1 cut(s) 232
BstF5I GGATG 5 cut(s) 135, 205, 319, 346, 358
BstKTI GATC 1 cut(s) 388
BstMAI GTCTC 2 cut(s) 155, 500
BstMBI GATC 1 cut(s) 385
BstMWI GCNNNNNNNGC 1 cut(s) 404
BstNSI RCATGY 1 cut(s) 331
BstSFI CTRYAG 1 cut(s) 408
BstV1I GCAGC 2 cut(s) 67, 394
BstV2I GAAGAC 1 cut(s) 425
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuRI GGCC 1 cut(s) 507
BtgZI GCGATG 2 cut(s) 308, 514
BtsCI GGATG 5 cut(s) 135, 205, 319, 346, 358
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 32
Cfr10I RCCGGY 1 cut(s) 30
Cfr13I GGNCC 1 cut(s) 292
Csp6I GTAC 1 cut(s) 460
CviAII CATG 1 cut(s) 328
CviJI RGCY 9 cut(s) 7, 30, 34, 109, 115, 186, 427, 507, 517
CviKI_1 RGCY 9 cut(s) 7, 30, 34, 109, 115, 186, 427, 507, 517
CviQI GTAC 1 cut(s) 460
DdeI CTNAG 1 cut(s) 347
DpnI GATC 1 cut(s) 387
DpnII GATC 1 cut(s) 385
Eam1104I CTCTTC 1 cut(s) 447
EarI CTCTTC 1 cut(s) 447
Eco130I CCWWGG 1 cut(s) 537
Eco147I AGGCCT 1 cut(s) 507
Eco31I GGTCTC 1 cut(s) 500
Eco47I GGWCC 1 cut(s) 292
Eco57I CTGAAG 1 cut(s) 131
EcoNI CCTNNNNNAGG 1 cut(s) 232
EcoT14I CCWWGG 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 537
FaeI CATG 1 cut(s) 331
FaiI YATR 4 cut(s) 105, 266, 329, 501
FaqI GGGAC 2 cut(s) 210, 305
FatI CATG 1 cut(s) 327
Fnu4HI GCNGC 2 cut(s) 81, 408
FokI GGATG 5 cut(s) 122, 212, 326, 353, 365
Fsp4HI GCNGC 2 cut(s) 81, 408
GluI GCNGC 2 cut(s) 81, 408
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 1 cut(s) 507
HapII CCGG 1 cut(s) 31
Hin1II CATG 1 cut(s) 331
HindIII AAGCTT 2 cut(s) 113, 425
HinfI GANTC 3 cut(s) 58, 150, 220
HpaII CCGG 1 cut(s) 31
Hpy188I TCNGA 2 cut(s) 63, 149
Hpy188III TCNNGA 4 cut(s) 74, 139, 212, 486
HpyAV CCTTC 3 cut(s) 371, 485, 497
HpyCH4III ACNGT 2 cut(s) 166, 193
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 2 cut(s) 374, 410
HpyF10VI GCNNNNNNNGC 1 cut(s) 404
HpyF3I CTNAG 1 cut(s) 347
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 1 cut(s) 331
KroI GCCGGC 1 cut(s) 30
KroNI GCCGGC 1 cut(s) 32
Kzo9I GATC 1 cut(s) 385
LpnPI CCDG 5 cut(s) 44, 69, 197, 274, 471
Lsp1109I GCAGC 2 cut(s) 67, 394
LweI GCATC 2 cut(s) 67, 343
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 2 cut(s) 127, 318
MalI GATC 1 cut(s) 387
MboI GATC 1 cut(s) 385
MboII GAAGA 3 cut(s) 380, 425, 434
MflI RGATCY 1 cut(s) 385
MluCI AATT 1 cut(s) 10
MmeI TCCRAC 1 cut(s) 546
MnlI CCTC 5 cut(s) 48, 228, 303, 331, 342
MroNI GCCGGC 1 cut(s) 30
MseI TTAA 3 cut(s) 9, 551, 565
MspI CCGG 1 cut(s) 31
Mva1269I GAATGC 1 cut(s) 409
MwoI GCNNNNNNNGC 1 cut(s) 404
NaeI GCCGGC 1 cut(s) 32
NdeII GATC 1 cut(s) 385
NgoMIV GCCGGC 1 cut(s) 30
NlaIII CATG 1 cut(s) 331
NlaIV GGNNCC 1 cut(s) 293
NmuCI GTSAC 1 cut(s) 127
NspI RCATGY 1 cut(s) 331
PceI AGGCCT 1 cut(s) 507
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PctI GAATGC 1 cut(s) 409
PdiI GCCGGC 1 cut(s) 32
PfeI GAWTC 3 cut(s) 58, 150, 220
PkrI GCNGC 2 cut(s) 82, 409
PspN4I GGNNCC 1 cut(s) 293
PspPI GGNCC 1 cut(s) 292
PstI CTGCAG 1 cut(s) 412
PsuI RGATCY 1 cut(s) 385
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
SaqAI TTAA 3 cut(s) 9, 551, 565
SatI GCNGC 2 cut(s) 81, 408
Sau3AI GATC 1 cut(s) 385
Sau96I GGNCC 1 cut(s) 292
SetI ASST 9 cut(s) 73, 111, 117, 159, 188, 262, 429, 496, 543
SfaNI GCATC 2 cut(s) 67, 343
SfcI CTRYAG 1 cut(s) 408
SinI GGWCC 1 cut(s) 292
SmlI CTYRAG 1 cut(s) 255
SmoI CTYRAG 1 cut(s) 255
Sse9I AATT 1 cut(s) 10
SseBI AGGCCT 1 cut(s) 507
StuI AGGCCT 1 cut(s) 507
StyI CCWWGG 1 cut(s) 537
TaaI ACNGT 2 cut(s) 166, 193
TaiI ACGT 1 cut(s) 159
TaqII GACCGA 1 cut(s) 309
TasI AATT 1 cut(s) 10
TfiI GAWTC 3 cut(s) 58, 150, 220
Tru1I TTAA 3 cut(s) 9, 551, 565
Tru9I TTAA 3 cut(s) 9, 551, 565
TscAI CASTG 1 cut(s) 196
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 2 cut(s) 80, 407
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 3 cut(s) 357, 416, 516
TspRI CASTG 1 cut(s) 196
VpaK11BI GGWCC 1 cut(s) 292
XagI CCTNNNNNAGG 1 cut(s) 232
XceI RCATGY 1 cut(s) 331
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.