Rw2G014750
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000844
Physical Location & Seq
Reverse (-)
16860951 .. 16862173
1223 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G014750.1

Sequence Viewer

Length: 546 bp
ATGGGTATGGCAATATCTAGGATGTTGAAGATGCTGTTTGCAAGGAAAGAAATGAGAATACTAATGGTGGGTCTTGATGCTGCTGGTAAAACCACCATCCTCTACAAGCTCAAACTTGGAGAGGTTGTCACTACCATACCCACAATTGGGTTCAATGTGGAAACTGTGGAATACAAAAATGTAAGCTTCACTGTCTGGGATGTAGGAGGACAAGACAAGATTCGGCCACTTTGGAGACACTATTTCCAGAACACTCAGGGTCTTATCTTTGTGGTGGATAGCAATGACAGAGACAGAGTTTCAGAAGCTAGAGACGAGCTCCATCGAATGCTAAGTGAGGATGAACTCTCCAATGCAACACTACTTGTATTTGCCAACAAGCAAGATCTTCCAAATGCCATGAGTGTTTCTGAGATCACTGATAAGCTTGGCCTGCATTCACTCCGCCAGCGCCGCTGGTACATTCAGGCTGCTTGTGCCACTTCTGGGCAAGGACTTTACGAGGGTCTTGATTGGTTATCCAGCAACATCTCAAGCAAAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko05110 ko05134 map04072 map04144 map05110 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

181

Amino Acids

20.54

Weight (kDa)

7.85

Isoelectric Point (pI)

43.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 6 - 177 1.2e-80 ADP-ribosylation factor family
SRPRB PF09439 17 - 143 1.7e-13 Signal recognition particle receptor beta subunit
Roc PF08477 19 - 129 7.3e-14 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 19 - 138 2.1e-12 Ras family
Gtr1_RagA PF04670 19 - 138 4.3e-10 Gtr1/RagA G protein conserved region
G-alpha PF00503 45 - 129 9.1e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 445, 454
AcoI YGGCCR 1 cut(s) 224
AfaI GTAC 1 cut(s) 461
AfiI CCNNNNNNNGG 3 cut(s) 146, 147, 486
AgsI TTSAA 2 cut(s) 28, 154
AluBI AGCT 5 cut(s) 109, 186, 308, 319, 427
AluI AGCT 5 cut(s) 109, 186, 308, 319, 427
Alw21I GWGCWC 1 cut(s) 321
Alw26I GTCTC 3 cut(s) 229, 285, 306
AoxI GGCC 2 cut(s) 224, 430
ApeKI GCWGC 2 cut(s) 80, 470
AspLEI GCGC 1 cut(s) 453
BanII GRGCYC 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 321
BbvI GCAGC 2 cut(s) 67, 457
BccI CCATC 2 cut(s) 104, 330
BcoDI GTCTC 3 cut(s) 229, 285, 306
BfaI CTAG 2 cut(s) 18, 309
BfoI RGCGCY 1 cut(s) 454
BglII AGATCT 1 cut(s) 385
BisI GCNGC 3 cut(s) 81, 454, 471
BlsI GCNGC 3 cut(s) 82, 455, 472
BmsI GCATC 2 cut(s) 21, 67
BplI GAGNNNNNCTC 2 cut(s) 303, 335
BpuEI CTTGAG 1 cut(s) 517
BsaXI ACNNNNNCTCC 2 cut(s) 111, 141
Bsc4I CCNNNNNNNGG 3 cut(s) 146, 147, 486
Bse3DI GCAATG 1 cut(s) 289
BseGI GGATG 4 cut(s) 27, 96, 205, 346
BseLI CCNNNNNNNGG 3 cut(s) 146, 147, 486
BseMI GCAATG 1 cut(s) 289
BseMII CTCAG 2 cut(s) 269, 402
BseXI GCAGC 2 cut(s) 67, 457
BshFI GGCC 2 cut(s) 226, 432
BsiHKAI GWGCWC 1 cut(s) 321
BslI CCNNNNNNNGG 3 cut(s) 146, 147, 486
BsmAI GTCTC 3 cut(s) 229, 285, 306
BsmBI CGTCTC 1 cut(s) 306
BsmI GAATGC 2 cut(s) 333, 436
BsnI GGCC 2 cut(s) 226, 432
Bsp1286I GDGCHC 1 cut(s) 321
Bsp143I GATC 2 cut(s) 385, 414
BspACI CCGC 2 cut(s) 445, 454
BspANI GGCC 2 cut(s) 226, 432
BspCNI CTCAG 2 cut(s) 268, 403
BsrDI GCAATG 1 cut(s) 289
BssMI GATC 2 cut(s) 385, 414
Bst4CI ACNGT 2 cut(s) 166, 193
BstC8I GCNNGC 2 cut(s) 434, 449
BstDEI CTNAG 3 cut(s) 255, 332, 411
BstF5I GGATG 4 cut(s) 27, 96, 205, 346
BstH2I RGCGCY 1 cut(s) 454
BstHHI GCGC 1 cut(s) 453
BstKTI GATC 2 cut(s) 388, 417
BstMAI GTCTC 3 cut(s) 229, 285, 306
BstMBI GATC 2 cut(s) 385, 414
BstMWI GCNNNNNNNGC 3 cut(s) 433, 453, 476
BstV1I GCAGC 2 cut(s) 67, 457
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuRI GGCC 2 cut(s) 226, 432
BtsCI GGATG 4 cut(s) 27, 96, 205, 346
BtsIMutI CAGTG 2 cut(s) 189, 417
Cac8I GCNNGC 2 cut(s) 434, 449
CfoI GCGC 1 cut(s) 453
Csp6I GTAC 1 cut(s) 460
CviAII CATG 2 cut(s) 400, 543
CviJI RGCY 8 cut(s) 109, 186, 226, 308, 319, 427, 432, 470
CviKI_1 RGCY 8 cut(s) 109, 186, 226, 308, 319, 427, 432, 470
CviQI GTAC 1 cut(s) 460
DdeI CTNAG 3 cut(s) 255, 332, 411
DpnI GATC 2 cut(s) 387, 416
DpnII GATC 2 cut(s) 385, 414
EaeI YGGCCR 1 cut(s) 224
EciI GGCGGA 1 cut(s) 434
Ecl136II GAGCTC 1 cut(s) 319
Eco24I GRGCYC 1 cut(s) 321
Eco53kI GAGCTC 1 cut(s) 319
EcoICRI GAGCTC 1 cut(s) 319
EcoT38I GRGCYC 1 cut(s) 321
Esp3I CGTCTC 1 cut(s) 306
FaeI CATG 2 cut(s) 403, 546
FaiI YATR 4 cut(s) 8, 137, 401, 544
FatI CATG 2 cut(s) 399, 542
Fnu4HI GCNGC 3 cut(s) 81, 454, 471
FokI GGATG 4 cut(s) 34, 83, 212, 353
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 3 cut(s) 81, 454, 471
FspBI CTAG 2 cut(s) 18, 309
GlaI GCGC 1 cut(s) 452
GluI GCNGC 3 cut(s) 81, 454, 471
HaeII RGCGCY 1 cut(s) 454
HaeIII GGCC 2 cut(s) 226, 432
HhaI GCGC 1 cut(s) 453
Hin1II CATG 2 cut(s) 403, 546
Hin6I GCGC 1 cut(s) 451
HinP1I GCGC 1 cut(s) 451
HindIII AAGCTT 2 cut(s) 184, 425
HinfI GANTC 1 cut(s) 220
Hpy188I TCNGA 2 cut(s) 304, 412
Hpy188III TCNNGA 3 cut(s) 74, 247, 509
HpyCH4III ACNGT 2 cut(s) 166, 193
HpyCH4V TGCA 3 cut(s) 41, 356, 436
HpyF10VI GCNNNNNNNGC 3 cut(s) 433, 453, 476
HpyF3I CTNAG 3 cut(s) 255, 332, 411
Hsp92II CATG 2 cut(s) 403, 546
HspAI GCGC 1 cut(s) 451
Kzo9I GATC 2 cut(s) 385, 414
LmnI GCTCC 1 cut(s) 324
Lsp1109I GCAGC 2 cut(s) 67, 457
LweI GCATC 2 cut(s) 21, 67
MaeI CTAG 2 cut(s) 18, 309
MaeIII GTNAC 1 cut(s) 127
MalI GATC 2 cut(s) 387, 416
MboI GATC 2 cut(s) 385, 414
MboII GAAGA 2 cut(s) 40, 380
MfeI CAATTG 1 cut(s) 144
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 1 cut(s) 321
MluCI AATT 1 cut(s) 144
MnlI CCTC 5 cut(s) 110, 115, 200, 331, 496
MspA1I CMGCKG 1 cut(s) 456
MunI CAATTG 1 cut(s) 144
Mva1269I GAATGC 2 cut(s) 333, 436
MwoI GCNNNNNNNGC 3 cut(s) 433, 453, 476
NdeII GATC 2 cut(s) 385, 414
NlaIII CATG 2 cut(s) 403, 546
NmuCI GTSAC 1 cut(s) 127
PctI GAATGC 2 cut(s) 333, 436
PfeI GAWTC 1 cut(s) 220
PkrI GCNGC 3 cut(s) 82, 455, 472
Psp124BI GAGCTC 1 cut(s) 321
PsuI RGATCY 1 cut(s) 385
RsaI GTAC 1 cut(s) 461
RsaNI GTAC 1 cut(s) 460
SacI GAGCTC 1 cut(s) 321
SatI GCNGC 3 cut(s) 81, 454, 471
Sau3AI GATC 2 cut(s) 385, 414
SduI GDGCHC 1 cut(s) 321
SetI ASST 6 cut(s) 111, 126, 188, 310, 321, 429
SfaNI GCATC 2 cut(s) 21, 67
SmlI CTYRAG 1 cut(s) 532
SmoI CTYRAG 1 cut(s) 532
Sse9I AATT 1 cut(s) 144
SsiI CCGC 2 cut(s) 445, 454
SspMI CTAG 2 cut(s) 18, 309
SstI GAGCTC 1 cut(s) 321
TaaI ACNGT 2 cut(s) 166, 193
TaqI TCGA 1 cut(s) 325
TasI AATT 1 cut(s) 144
TauI GCSGC 1 cut(s) 456
TfiI GAWTC 1 cut(s) 220
TscAI CASTG 2 cut(s) 196, 424
TseFI GTSAC 1 cut(s) 127
TseI GCWGC 2 cut(s) 80, 470
Tsp45I GTSAC 1 cut(s) 127
TspDTI ATGAA 1 cut(s) 357
TspRI CASTG 2 cut(s) 196, 424
XspI CTAG 2 cut(s) 18, 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.