Rorug05G0559300

ADP-ribosylation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000844
Physical Location & Seq
Forward (+)
74886923 .. 74889536
2614 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0559300.1

Sequence Viewer

Length: 1011 bp
ATGACAGATCCCTACCCATCTAATTACTTCAATGGTTGGTTCAATCTCAACTTCTCTCCCCCCACACCTTCTCTTGCCTATAATTTTCATCATCATCATCAAGCTCCTTTCTCAAATTATAACATGATTAACAACAATAGCAATAGCTTTTTCAGTAGCCATCACCAATCTTCATCATCACCTCCCTCTCCTCCTTTGAAAGAAGCCCTTCCTCTACTCAGCTTAAGCCCTAAAAGAGATCATCAAGATGAATTAGCTGAGGATCATTATCAGCATGCTGATGATAATGATGATGATGAAGAGGAAACTGTTACTGTGGCTTTGCATTTAGGGCTACCAAACCCTAGCTCTGCTGCTGATTTAATCTCAAGGCTCTCCTCCTCAGAGATCTCATCTGCAGATAGAGAAGATGCAGTCACTACTCCTAATGCTTCTTCACCTGGGTATGTATCAAACAGACTCAACAAGGGCCAGTATTGGATCCCAACACCTTCTCAAATTTTAATTGGTCCAACCCAGTTCTCTTGCCCCCTTTGCTTCAAGACCTTCAACAGATACAATAACATGCAGATGCATATGTGGGGGCATGGATCTCAATACAGAAAAGGGCCAGAGTCTCTAAGAGGAACCCAACCAACAGCCATGCTTAGACTCCCTTGCTATTGCTGTGCACCAGGTTGCAGGAACAACATTGACCATCCTAGGTCAAAGCCTCTCAAAGACTTCAGAACCCTCCAAACCCATTACAAGAGAAAACATGGGATCAAGCCTTTCATGTGTAGGAAATGTGGCAAGGCGTTTGCGGTGAGGGGTGATTGGAGAACCCATGAGAAAAATTGCGGCAAGCTTTGGTACTGCACTTGTGGCTCGGATTTCAAGCACAAGAGGTCTCTCAAGGATCATATCAAGGCTTTTGGACAAGGCCATGCAGCTTATGGCATTGATTGTTTTGAAGAAGAAGAAGATGATGATGCAGCCTCTGAAATTGAGGAAAATGACTCTTGCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko05110 ko05134 map04072 map04144 map05110 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

336

Amino Acids

38.09

Weight (kDa)

6.43

Isoelectric Point (pI)

56.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C2H2_STOP2_3rd PF23115 220 - 254 1e-18 STOP2-like, C2H2-type zinc finger
C2CH-3rd_BIRD-IDD PF22995 257 - 281 6.6e-12 BIRD-IDD transcription factor, third C2HC zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 120
AciI CCGC 2 cut(s) 803, 840
AclWI GGATC 7 cut(s) 2, 270, 475, 488, 598, 770, 906
AcsI RAATTY 1 cut(s) 498
AcuI CTGAAG 1 cut(s) 709
AfaI GTAC 1 cut(s) 854
AflII CTTAAG 1 cut(s) 223
AgsI TTSAA 7 cut(s) 31, 43, 199, 541, 550, 877, 953
AjnI CCWGG 2 cut(s) 439, 673
AluBI AGCT 7 cut(s) 104, 147, 222, 257, 348, 847, 932
AluI AGCT 7 cut(s) 104, 147, 222, 257, 348, 847, 932
Alw21I GWGCWC 1 cut(s) 673
Alw26I GTCTC 2 cut(s) 621, 894
Alw44I GTGCAC 1 cut(s) 669
AlwI GGATC 7 cut(s) 2, 270, 475, 488, 598, 770, 906
AlwNI CAGNNNCTG 1 cut(s) 980
AoxI GGCC 3 cut(s) 469, 608, 922
ApaLI GTGCAC 1 cut(s) 669
ApeKI GCWGC 3 cut(s) 353, 929, 974
ApoI RAATTY 1 cut(s) 498
Asp700I GAANNNNTTC 1 cut(s) 207
AspA2I CCTAGG 1 cut(s) 701
AspS9I GGNCC 3 cut(s) 469, 509, 608
AsuHPI GGTGA 5 cut(s) 155, 171, 429, 817, 824
AvaII GGWCC 1 cut(s) 509
AvrII CCTAGG 1 cut(s) 701
BaeGI GKGCMC 1 cut(s) 673
BamHI GGATCC 1 cut(s) 480
Bbv12I GWGCWC 1 cut(s) 673
BbvCI CCTCAGC 1 cut(s) 258
BbvI GCAGC 3 cut(s) 340, 941, 986
BccI CCATC 3 cut(s) 25, 168, 705
BciT130I CCWGG 2 cut(s) 441, 675
BcoDI GTCTC 2 cut(s) 621, 894
BfaI CTAG 2 cut(s) 345, 702
BfmI CTRYAG 1 cut(s) 396
BfrI CTTAAG 1 cut(s) 223
BglII AGATCT 1 cut(s) 387
BisI GCNGC 4 cut(s) 354, 841, 930, 975
BlnI CCTAGG 1 cut(s) 701
BlsI GCNGC 4 cut(s) 355, 842, 931, 976
Bme1390I CCNGG 2 cut(s) 441, 675
Bme18I GGWCC 1 cut(s) 509
BmgT120I GGNCC 3 cut(s) 469, 509, 608
BmiI GGNNCC 2 cut(s) 482, 628
BmrFI CCNGG 2 cut(s) 441, 675
BmrI ACTGGG 1 cut(s) 511
BmsI GCATC 3 cut(s) 400, 561, 961
BmuI ACTGGG 1 cut(s) 511
Bpu10I CCTNAGC 1 cut(s) 258
BpuEI CTTGAG 2 cut(s) 352, 878
BsaBI GATNNNNATC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 894
BsaJI CCNNGG 2 cut(s) 440, 701
Bse1I ACTGG 2 cut(s) 472, 517
Bse8I GATNNNNATC 1 cut(s) 267
BseBI CCWGG 2 cut(s) 441, 675
BseDI CCNNGG 2 cut(s) 440, 701
BseGI GGATG 1 cut(s) 697
BseJI GATNNNNATC 1 cut(s) 267
BseMII CTCAG 3 cut(s) 232, 249, 396
BseNI ACTGG 2 cut(s) 472, 517
BseRI GAGGAG 3 cut(s) 180, 367, 370
BseSI GKGCMC 1 cut(s) 673
BseXI GCAGC 3 cut(s) 340, 941, 986
BsgI GTGCAG 1 cut(s) 841
BshFI GGCC 3 cut(s) 471, 610, 924
BsiHKAI GWGCWC 1 cut(s) 673
BsmAI GTCTC 2 cut(s) 621, 894
BsnI GGCC 3 cut(s) 471, 610, 924
Bso31I GGTCTC 1 cut(s) 894
Bsp1286I GDGCHC 1 cut(s) 673
Bsp143I GATC 8 cut(s) 7, 238, 262, 387, 480, 590, 762, 898
BspACI CCGC 2 cut(s) 803, 840
BspANI GGCC 3 cut(s) 471, 610, 924
BspCNI CTCAG 3 cut(s) 231, 250, 395
BspLI GGNNCC 2 cut(s) 482, 628
BspMAI CTGCAG 1 cut(s) 400
BspPI GGATC 7 cut(s) 2, 270, 475, 488, 598, 770, 906
BspTI CTTAAG 1 cut(s) 223
BspTNI GGTCTC 1 cut(s) 894
BsrI ACTGG 2 cut(s) 472, 517
BssECI CCNNGG 2 cut(s) 440, 701
BssMI GATC 8 cut(s) 7, 238, 262, 387, 480, 590, 762, 898
BssT1I CCWWGG 1 cut(s) 701
Bst2UI CCWGG 2 cut(s) 441, 675
Bst4CI ACNGT 2 cut(s) 310, 316
Bst6I CTCTTC 1 cut(s) 294
BstAFI CTTAAG 1 cut(s) 223
BstC8I GCNNGC 2 cut(s) 276, 845
BstDEI CTNAG 5 cut(s) 218, 258, 382, 620, 647
BstF5I GGATG 1 cut(s) 697
BstKTI GATC 8 cut(s) 10, 241, 265, 390, 483, 593, 765, 901
BstMAI GTCTC 2 cut(s) 621, 894
BstMBI GATC 8 cut(s) 7, 238, 262, 387, 480, 590, 762, 898
BstMWI GCNNNNNNNGC 3 cut(s) 331, 534, 864
BstNI CCWGG 2 cut(s) 441, 675
BstNSI RCATGY 2 cut(s) 278, 568
BstSCI CCNGG 2 cut(s) 439, 673
BstSFI CTRYAG 1 cut(s) 396
BstSLI GKGCMC 1 cut(s) 673
BstV1I GCAGC 3 cut(s) 340, 941, 986
BstX2I RGATCY 4 cut(s) 7, 387, 480, 590
BstYI RGATCY 4 cut(s) 7, 387, 480, 590
BsuRI GGCC 3 cut(s) 471, 610, 924
BtsCI GGATG 1 cut(s) 697
Cac8I GCNNGC 2 cut(s) 276, 845
CaiI CAGNNNCTG 1 cut(s) 980
Cfr13I GGNCC 3 cut(s) 469, 509, 608
CsiI ACCWGGT 1 cut(s) 673
Csp6I GTAC 1 cut(s) 853
CviAII CATG 9 cut(s) 124, 275, 565, 587, 643, 758, 775, 827, 926
CviQI GTAC 1 cut(s) 853
DdeI CTNAG 5 cut(s) 218, 258, 382, 620, 647
DpnI GATC 8 cut(s) 9, 240, 264, 389, 482, 592, 764, 900
DpnII GATC 8 cut(s) 7, 238, 262, 387, 480, 590, 762, 898
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
Eco130I CCWWGG 1 cut(s) 701
Eco31I GGTCTC 1 cut(s) 894
Eco47I GGWCC 1 cut(s) 509
Eco57I CTGAAG 1 cut(s) 709
EcoRII CCWGG 2 cut(s) 439, 673
EcoT14I CCWWGG 1 cut(s) 701
EcoT22I ATGCAT 1 cut(s) 576
ErhI CCWWGG 1 cut(s) 701
FaeI CATG 9 cut(s) 127, 278, 568, 590, 646, 761, 778, 830, 929
FalI AAGNNNNNCTT 2 cut(s) 192, 224
FatI CATG 9 cut(s) 123, 274, 564, 586, 642, 757, 774, 826, 925
FauNDI CATATG 1 cut(s) 576
Fnu4HI GCNGC 4 cut(s) 354, 841, 930, 975
FokI GGATG 1 cut(s) 684
Fsp4HI GCNGC 4 cut(s) 354, 841, 930, 975
FspBI CTAG 2 cut(s) 345, 702
GluI GCNGC 4 cut(s) 354, 841, 930, 975
HaeIII GGCC 3 cut(s) 471, 610, 924
Hin1II CATG 9 cut(s) 127, 278, 568, 590, 646, 761, 778, 830, 929
HindIII AAGCTT 1 cut(s) 845
HinfI GANTC 4 cut(s) 459, 614, 651, 998
HphI GGTGA 5 cut(s) 155, 171, 429, 817, 824
Hpy166II GTNNAC 1 cut(s) 671
Hpy188I TCNGA 4 cut(s) 385, 728, 871, 982
Hpy188III TCNNGA 2 cut(s) 245, 541
Hpy8I GTNNAC 1 cut(s) 671
HpyAV CCTTC 4 cut(s) 78, 218, 501, 556
HpyCH4III ACNGT 2 cut(s) 310, 316
HpyF10VI GCNNNNNNNGC 3 cut(s) 331, 534, 864
HpyF3I CTNAG 5 cut(s) 218, 258, 382, 620, 647
Hsp92II CATG 9 cut(s) 127, 278, 568, 590, 646, 761, 778, 830, 929
Kzo9I GATC 8 cut(s) 7, 238, 262, 387, 480, 590, 762, 898
LmnI GCTCC 1 cut(s) 109
LpnPI CCDG 8 cut(s) 426, 453, 485, 530, 624, 660, 667, 687
Lsp1109I GCAGC 3 cut(s) 340, 941, 986
LweI GCATC 3 cut(s) 400, 561, 961
MabI ACCWGGT 1 cut(s) 673
MaeI CTAG 2 cut(s) 345, 702
MaeIII GTNAC 2 cut(s) 310, 415
MalI GATC 8 cut(s) 9, 240, 264, 389, 482, 592, 764, 900
MboI GATC 8 cut(s) 7, 238, 262, 387, 480, 590, 762, 898
MboII GAAGA 8 cut(s) 162, 311, 419, 426, 965, 968, 971, 974
MflI RGATCY 4 cut(s) 7, 387, 480, 590
MhlI GDGCHC 1 cut(s) 673
MluCI AATT 8 cut(s) 22, 82, 115, 251, 498, 504, 835, 984
MlyI GAGTC 4 cut(s) 453, 623, 645, 992
MmeI TCCRAC 1 cut(s) 536
Mph1103I ATGCAT 1 cut(s) 576
MroXI GAANNNNTTC 1 cut(s) 207
MseI TTAA 4 cut(s) 129, 224, 362, 503
MslI CAYNNNNRTG 3 cut(s) 246, 279, 569
MspCI CTTAAG 1 cut(s) 223
MspR9I CCNGG 2 cut(s) 441, 675
MvaI CCWGG 2 cut(s) 441, 675
MwoI GCNNNNNNNGC 3 cut(s) 331, 534, 864
NdeI CATATG 1 cut(s) 576
NdeII GATC 8 cut(s) 7, 238, 262, 387, 480, 590, 762, 898
NlaIII CATG 9 cut(s) 127, 278, 568, 590, 646, 761, 778, 830, 929
NlaIV GGNNCC 2 cut(s) 482, 628
NmuCI GTSAC 1 cut(s) 415
NsiI ATGCAT 1 cut(s) 576
NspI RCATGY 2 cut(s) 278, 568
PaeI GCATGC 1 cut(s) 278
PdmI GAANNNNTTC 1 cut(s) 207
PkrI GCNGC 4 cut(s) 355, 842, 931, 976
PleI GAGTC 4 cut(s) 453, 622, 645, 992
PpsI GAGTC 4 cut(s) 453, 622, 645, 992
PsiI TTATAA 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 439, 673
PspGI CCWGG 2 cut(s) 439, 673
PspN4I GGNNCC 2 cut(s) 482, 628
PspPI GGNCC 3 cut(s) 469, 509, 608
PstI CTGCAG 1 cut(s) 400
PstNI CAGNNNCTG 1 cut(s) 980
PsuI RGATCY 4 cut(s) 7, 387, 480, 590
RsaI GTAC 1 cut(s) 854
RsaNI GTAC 1 cut(s) 853
RseI CAYNNNNRTG 3 cut(s) 246, 279, 569
SaqAI TTAA 4 cut(s) 129, 224, 362, 503
SatI GCNGC 4 cut(s) 354, 841, 930, 975
Sau3AI GATC 8 cut(s) 7, 238, 262, 387, 480, 590, 762, 898
Sau96I GGNCC 3 cut(s) 469, 509, 608
SchI GAGTC 4 cut(s) 453, 623, 645, 992
ScrFI CCNGG 2 cut(s) 441, 675
SduI GDGCHC 1 cut(s) 673
SexAI ACCWGGT 1 cut(s) 673
SfaNI GCATC 3 cut(s) 400, 561, 961
SfcI CTRYAG 1 cut(s) 396
SinI GGWCC 1 cut(s) 509
SmiMI CAYNNNNRTG 3 cut(s) 246, 279, 569
SmlI CTYRAG 3 cut(s) 223, 367, 893
SmoI CTYRAG 3 cut(s) 223, 367, 893
SphI GCATGC 1 cut(s) 278
Sse9I AATT 8 cut(s) 22, 82, 115, 251, 498, 504, 835, 984
SsiI CCGC 2 cut(s) 803, 840
SspMI CTAG 2 cut(s) 345, 702
StyD4I CCNGG 2 cut(s) 439, 673
StyI CCWWGG 1 cut(s) 701
TaaI ACNGT 2 cut(s) 310, 316
TasI AATT 8 cut(s) 22, 82, 115, 251, 498, 504, 835, 984
TauI GCSGC 1 cut(s) 843
Tru1I TTAA 4 cut(s) 129, 224, 362, 503
Tru9I TTAA 4 cut(s) 129, 224, 362, 503
TseFI GTSAC 1 cut(s) 415
TseI GCWGC 3 cut(s) 353, 929, 974
Tsp45I GTSAC 1 cut(s) 415
TspDTI ATGAA 5 cut(s) 77, 162, 264, 312, 763
Vha464I CTTAAG 1 cut(s) 223
VneI GTGCAC 1 cut(s) 669
VpaK11BI GGWCC 1 cut(s) 509
XapI RAATTY 1 cut(s) 498
XceI RCATGY 2 cut(s) 278, 568
XcmI CCANNNNNNNNNTGG 1 cut(s) 932
XmaJI CCTAGG 1 cut(s) 701
XmnI GAANNNNTTC 1 cut(s) 207
XspI CTAG 2 cut(s) 345, 702
Zsp2I ATGCAT 1 cut(s) 576
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.