MD13G1093800.v1.1
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000844
Physical Location & Seq
Reverse (-)
6611354 .. 6614747
3394 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1093800.v1.1.491

Sequence Viewer

Length: 786 bp
ATGACCAGTGACATGGCGGGCACTGATTGGACACACAATGTCACCCGCAGGCTTAGCGTTGTAGGGGCATTTTCGTACTTCAAGCCAATTACGAAAAAAATAGTCGAGTTTATAAACCCCCAGTTGACAATCCTCACCCCTACACATTCACGCGTTGGTTCAAAGCTTCCGTCTCCTTCTCTCTCTCTCTCTCCCTCGATCTCCATCTTCAAGATCCCTCTGCGAAAGCTCATCGAAGAAATGGGGCTTACATTCACGAAGCTTTTCAGCCGGCTTTTTGCCAAGAAGGAGATGCGAATTCTGATGGTCGGTCTTGATGCCGCCGGTAAGACCACCATCCTCTACAAGCTCAAGCTTGGTGAGATCGTCACAACCATTCCCACCATTGGGTTTAATGTGGAGACTGTGGAGTACAAGAACATCAGCTTCACTGTCTGGGATGTCGGGGGTCAGGACAAGATCCGACCATTGTGGAGGCACTACTTCCAAAACACACAGGGTCTTATCTTTGTTGTGGACAGCAATGACAGAGACCGTGTTGTTGAGGCAAGGGATGAATTGCATAGGATGTTGAATGAGGATGAACTGCGTGATGCTGTGTTGCTCGTATTTGCTAATAAACAAGATCTTCCCAATGCAATGAATGCTGCCGAAATCACTGATAAGCTTGGCCTGCACTCTCTGAGACAGCGTCACTGGTACATTCAGAGCACCTGTGCAACCTCTGGGGAGGGTCTATATGAGGGGCTCGATTGGCTCTCCAACAACATTGCTAACAAGGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko05110 ko05134 map04072 map04144 map05110 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

262

Amino Acids

29.6

Weight (kDa)

9.08

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 85 - 257 4.3e-81 ADP-ribosylation factor family
SRPRB PF09439 96 - 223 1.4e-12 Signal recognition particle receptor beta subunit
Roc PF08477 99 - 209 2.1e-13 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 99 - 253 1.6e-11 Ras family
Gtr1_RagA PF04670 99 - 220 4.6e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 125 - 191 1.7e-09 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 113
AccII CGCG 1 cut(s) 153
AciI CCGC 3 cut(s) 17, 46, 321
AclWI GGATC 2 cut(s) 208, 454
AcsI RAATTY 1 cut(s) 297
AfaI GTAC 3 cut(s) 77, 413, 701
AfiI CCNNNNNNNGG 2 cut(s) 386, 387
AflIII ACRYGT 1 cut(s) 151
AgsI TTSAA 4 cut(s) 82, 162, 211, 574
AluBI AGCT 7 cut(s) 166, 229, 262, 349, 355, 426, 667
AluI AGCT 7 cut(s) 166, 229, 262, 349, 355, 426, 667
Alw21I GWGCWC 1 cut(s) 713
Alw26I GTCTC 4 cut(s) 177, 395, 525, 679
AlwI GGATC 2 cut(s) 208, 454
AoxI GGCC 1 cut(s) 670
ApeKI GCWGC 1 cut(s) 647
ApoI RAATTY 1 cut(s) 297
ArsI GACNNNNNNTTYG 2 cut(s) 155, 187
Asp700I GAANNNNTTC 1 cut(s) 263
AsuHPI GGTGA 3 cut(s) 34, 127, 371
BaeGI GKGCMC 1 cut(s) 23
BanII GRGCYC 1 cut(s) 750
Bbv12I GWGCWC 1 cut(s) 713
BbvI GCAGC 1 cut(s) 634
BccI CCATC 3 cut(s) 212, 298, 344
BcoDI GTCTC 4 cut(s) 177, 395, 525, 679
BglII AGATCT 1 cut(s) 625
BisI GCNGC 2 cut(s) 321, 648
BlpI GCTNAGC 1 cut(s) 53
BlsI GCNGC 2 cut(s) 322, 649
BmrI ACTGGG 1 cut(s) 115
BmsI GCATC 3 cut(s) 282, 307, 583
BmuI ACTGGG 1 cut(s) 115
Bpu1102I GCTNAGC 1 cut(s) 53
BpuEI CTTGAG 1 cut(s) 335
BsaBI GATNNNNATC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 525
Bsc4I CCNNNNNNNGG 2 cut(s) 386, 387
Bse118I RCCGGY 2 cut(s) 270, 323
Bse1I ACTGG 3 cut(s) 6, 121, 701
Bse3DI GCAATG 3 cut(s) 529, 645, 768
Bse8I GATNNNNATC 1 cut(s) 203
BseGI GGATG 5 cut(s) 336, 445, 559, 573, 586
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 2 cut(s) 386, 387
BseMI GCAATG 3 cut(s) 529, 645, 768
BseMII CTCAG 1 cut(s) 674
BseNI ACTGG 3 cut(s) 6, 121, 701
BseSI GKGCMC 1 cut(s) 23
BseXI GCAGC 1 cut(s) 634
BsgI GTGCAG 1 cut(s) 659
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 1 cut(s) 672
BsiHKAI GWGCWC 1 cut(s) 713
BsiSI CCGG 2 cut(s) 271, 324
BslI CCNNNNNNNGG 2 cut(s) 386, 387
BsmAI GTCTC 4 cut(s) 177, 395, 525, 679
BsmBI CGTCTC 1 cut(s) 177
BsmI GAATGC 1 cut(s) 649
BsnI GGCC 1 cut(s) 672
Bso31I GGTCTC 1 cut(s) 525
Bsp1286I GDGCHC 3 cut(s) 23, 713, 750
Bsp143I GATC 5 cut(s) 198, 213, 363, 459, 625
Bsp1720I GCTNAGC 1 cut(s) 53
BspACI CCGC 3 cut(s) 17, 46, 321
BspANI GGCC 1 cut(s) 672
BspCNI CTCAG 1 cut(s) 675
BspFNI CGCG 1 cut(s) 153
BspPI GGATC 2 cut(s) 208, 454
BspTNI GGTCTC 1 cut(s) 525
BsrDI GCAATG 3 cut(s) 529, 645, 768
BsrFI RCCGGY 2 cut(s) 270, 323
BsrI ACTGG 3 cut(s) 6, 121, 701
BssAI RCCGGY 2 cut(s) 270, 323
BssMI GATC 5 cut(s) 198, 213, 363, 459, 625
Bst4CI ACNGT 3 cut(s) 406, 433, 536
BstAPI GCANNNNNTGC 1 cut(s) 644
BstC8I GCNNGC 4 cut(s) 19, 50, 272, 674
BstDEI CTNAG 2 cut(s) 53, 683
BstF5I GGATG 5 cut(s) 336, 445, 559, 573, 586
BstFNI CGCG 1 cut(s) 153
BstKTI GATC 5 cut(s) 201, 216, 366, 462, 628
BstMAI GTCTC 4 cut(s) 177, 395, 525, 679
BstMBI GATC 5 cut(s) 198, 213, 363, 459, 625
BstMWI GCNNNNNNNGC 5 cut(s) 54, 644, 673, 754, 779
BstSLI GKGCMC 1 cut(s) 23
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 1 cut(s) 634
BstX2I RGATCY 3 cut(s) 213, 459, 625
BstXI CCANNNNNNTGG 1 cut(s) 13
BstYI RGATCY 3 cut(s) 213, 459, 625
BsuRI GGCC 1 cut(s) 672
BtsCI GGATG 5 cut(s) 336, 445, 559, 573, 586
BtsIMutI CAGTG 5 cut(s) 13, 21, 429, 657, 694
Cac8I GCNNGC 4 cut(s) 19, 50, 272, 674
Cfr10I RCCGGY 2 cut(s) 270, 323
CseI GACGC 1 cut(s) 680
Csp6I GTAC 3 cut(s) 76, 412, 700
CviAII CATG 1 cut(s) 13
CviQI GTAC 3 cut(s) 76, 412, 700
DdeI CTNAG 2 cut(s) 53, 683
DpnI GATC 5 cut(s) 200, 215, 365, 461, 627
DpnII GATC 5 cut(s) 198, 213, 363, 459, 625
Eco24I GRGCYC 1 cut(s) 750
Eco31I GGTCTC 1 cut(s) 525
EcoRI GAATTC 1 cut(s) 297
EcoT38I GRGCYC 1 cut(s) 750
Esp3I CGTCTC 1 cut(s) 177
FaeI CATG 1 cut(s) 16
FaiI YATR 5 cut(s) 14, 113, 564, 739, 741
FatI CATG 1 cut(s) 12
FauI CCCGC 2 cut(s) 10, 53
Fnu4HI GCNGC 2 cut(s) 321, 648
FokI GGATG 5 cut(s) 323, 452, 566, 580, 593
FriOI GRGCYC 1 cut(s) 750
Fsp4HI GCNGC 2 cut(s) 321, 648
GluI GCNGC 2 cut(s) 321, 648
HaeIII GGCC 1 cut(s) 672
HapII CCGG 2 cut(s) 271, 324
HgaI GACGC 1 cut(s) 680
Hin1II CATG 1 cut(s) 16
HincII GTYRAC 1 cut(s) 126
HindII GTYRAC 1 cut(s) 126
HindIII AAGCTT 4 cut(s) 164, 260, 353, 665
HpaII CCGG 2 cut(s) 271, 324
HphI GGTGA 3 cut(s) 34, 127, 371
Hpy166II GTNNAC 2 cut(s) 126, 517
Hpy188I TCNGA 4 cut(s) 303, 464, 684, 708
Hpy188III TCNNGA 4 cut(s) 211, 256, 314, 452
Hpy8I GTNNAC 2 cut(s) 126, 517
HpyAV CCTTC 2 cut(s) 186, 280
HpyCH4III ACNGT 3 cut(s) 406, 433, 536
HpyCH4V TGCA 4 cut(s) 562, 638, 676, 719
HpyF10VI GCNNNNNNNGC 5 cut(s) 54, 644, 673, 754, 779
HpyF3I CTNAG 2 cut(s) 53, 683
Hsp92II CATG 1 cut(s) 16
KroI GCCGGC 1 cut(s) 270
KroNI GCCGGC 1 cut(s) 272
Kzo9I GATC 5 cut(s) 198, 213, 363, 459, 625
Lsp1109I GCAGC 1 cut(s) 634
LweI GCATC 3 cut(s) 282, 307, 583
MaeIII GTNAC 4 cut(s) 8, 40, 367, 692
MalI GATC 5 cut(s) 200, 215, 365, 461, 627
MboI GATC 5 cut(s) 198, 213, 363, 459, 625
MboII GAAGA 3 cut(s) 199, 248, 620
MflI RGATCY 3 cut(s) 213, 459, 625
MhlI GDGCHC 3 cut(s) 23, 713, 750
MluCI AATT 3 cut(s) 87, 297, 557
MluI ACGCGT 1 cut(s) 151
MmeI TCCRAC 2 cut(s) 487, 786
MroNI GCCGGC 1 cut(s) 270
MroXI GAANNNNTTC 1 cut(s) 263
MseI TTAA 1 cut(s) 393
MspI CCGG 2 cut(s) 271, 324
Mva1269I GAATGC 1 cut(s) 649
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 5 cut(s) 54, 644, 673, 754, 779
NaeI GCCGGC 1 cut(s) 272
NdeII GATC 5 cut(s) 198, 213, 363, 459, 625
NgoMIV GCCGGC 1 cut(s) 270
NlaIII CATG 1 cut(s) 16
NmuCI GTSAC 4 cut(s) 8, 40, 367, 692
PctI GAATGC 1 cut(s) 649
PdiI GCCGGC 1 cut(s) 272
PdmI GAANNNNTTC 1 cut(s) 263
PflFI GACNNNGTC 1 cut(s) 690
PkrI GCNGC 2 cut(s) 322, 649
PsiI TTATAA 1 cut(s) 113
PsuI RGATCY 3 cut(s) 213, 459, 625
PsyI GACNNNGTC 1 cut(s) 690
RsaI GTAC 3 cut(s) 77, 413, 701
RsaNI GTAC 3 cut(s) 76, 412, 700
SaqAI TTAA 1 cut(s) 393
SatI GCNGC 2 cut(s) 321, 648
Sau3AI GATC 5 cut(s) 198, 213, 363, 459, 625
SduI GDGCHC 3 cut(s) 23, 713, 750
SetI ASST 9 cut(s) 168, 231, 264, 351, 357, 428, 669, 716, 725
SfaNI GCATC 3 cut(s) 282, 307, 583
SmlI CTYRAG 1 cut(s) 350
SmoI CTYRAG 1 cut(s) 350
Sse9I AATT 3 cut(s) 87, 297, 557
SsiI CCGC 3 cut(s) 17, 46, 321
TaaI ACNGT 3 cut(s) 406, 433, 536
TaqI TCGA 4 cut(s) 105, 197, 234, 750
TaqII GACCGA 1 cut(s) 299
TasI AATT 3 cut(s) 87, 297, 557
TatI WGTACW 1 cut(s) 411
TauI GCSGC 1 cut(s) 323
Tru1I TTAA 1 cut(s) 393
Tru9I TTAA 1 cut(s) 393
TscAI CASTG 5 cut(s) 13, 28, 436, 664, 701
TseFI GTSAC 4 cut(s) 8, 40, 367, 692
TseI GCWGC 1 cut(s) 647
Tsp45I GTSAC 4 cut(s) 8, 40, 367, 692
TspDTI ATGAA 3 cut(s) 570, 597, 656
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 5 cut(s) 13, 28, 436, 664, 701
Tth111I GACNNNGTC 1 cut(s) 690
XapI RAATTY 1 cut(s) 297
XmnI GAANNNNTTC 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.