Rh2BG647400
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0000844
Physical Location & Seq
Reverse (-)
86653623 .. 86656592
2970 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG647400.1

Sequence Viewer

Length: 594 bp
ATGATCCTTCCACGGTTTCCTTCCTCCAGCAAAAGTTGGAAAAAAAAAATGGGGCTGACGTTCACGAAGCTCTTCAGCCGGCTTTTCGCCAAGAAAGAGATGCGAATTCTGATGGTCGGTCTCGACGCCGCCGGTAAGACCACCATCTTGTACAAGCTCAAGCTCGGAGAAATCGTCACCACCATCCCTACCATCGGATTTAATGTGGAGACTGTGGAATACAAGAACATCAGCTTCACTGTGTGGGATGTAGGGGGTCAGGACAAGATCCGTCCATTGTGGAGGCACTACTTCCAGAACACTCAAGGTCTTATTTTTGTGGTTGATAGCAATGACAGGGATCGAGTTGTTGAGGCGAGGGATGAGTTGCACAGGATGTTGAATGAGGATGAACTGAGGGATGCTGTTTTGCTTGTATTTGCCAACAAACAAGATCTTCCTAATGCAATGAATGCTGCAGAAATAACTGATAAGCTTGGTCTTCATTCTCTCCGCCAACGTCACTGGTACATTCAGAGCACATGTGCAACCTCTGGTGAGGGACTGTACGAGGGATTGGACTGGCTTTCCAACAACATTGCTAGCAAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko05110 ko05134 map04072 map04144 map05110 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

197

Amino Acids

22.57

Weight (kDa)

9.14

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 21 - 193 1.7e-81 ADP-ribosylation factor family
SRPRB PF09439 32 - 159 6.2e-13 Signal recognition particle receptor beta subunit
Roc PF08477 35 - 145 1e-13 Ras of Complex, Roc, domain of DAPkinase
Ras PF00071 35 - 188 7e-12 Ras family
Gtr1_RagA PF04670 35 - 157 2.1e-09 Gtr1/RagA G protein conserved region
G-alpha PF00503 61 - 127 9.7e-10 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 129, 493
AclWI GGATC 2 cut(s) 262, 348
AcsI RAATTY 1 cut(s) 105
AcuI CTGAAG 1 cut(s) 58
AcyI GRCGYC 1 cut(s) 126
AdeI CACNNNGTG 1 cut(s) 243
AfaI GTAC 3 cut(s) 152, 509, 548
AfiI CCNNNNNNNGG 1 cut(s) 194
AflIII ACRYGT 1 cut(s) 521
AgsI TTSAA 1 cut(s) 382
AluBI AGCT 5 cut(s) 70, 157, 163, 234, 475
AluI AGCT 5 cut(s) 70, 157, 163, 234, 475
Alw21I GWGCWC 1 cut(s) 521
Alw26I GTCTC 2 cut(s) 125, 203
AlwI GGATC 2 cut(s) 262, 348
ApeKI GCWGC 1 cut(s) 455
ApoI RAATTY 1 cut(s) 105
Asp700I GAANNNNTTC 1 cut(s) 71
AsuHPI GGTGA 2 cut(s) 169, 548
AsuNHI GCTAGC 1 cut(s) 581
BbsI GAAGAC 1 cut(s) 473
Bbv12I GWGCWC 1 cut(s) 521
BbvI GCAGC 1 cut(s) 442
BccI CCATC 4 cut(s) 106, 152, 191, 200
BcoDI GTCTC 2 cut(s) 125, 203
BfaI CTAG 1 cut(s) 582
BfmI CTRYAG 1 cut(s) 456
BglII AGATCT 1 cut(s) 433
BisI GCNGC 2 cut(s) 129, 456
BlsI GCNGC 2 cut(s) 130, 457
BmsI GCATC 2 cut(s) 90, 391
BmtI GCTAGC 1 cut(s) 585
BpiI GAAGAC 1 cut(s) 473
BpmI CTGGAG 1 cut(s) 10
BpuEI CTTGAG 2 cut(s) 143, 288
BsaHI GRCGYC 1 cut(s) 126
BsaI GGTCTC 1 cut(s) 125
BsaJI CCNNGG 1 cut(s) 11
BsaXI ACNNNNNCTCC 2 cut(s) 159, 189
Bsc4I CCNNNNNNNGG 1 cut(s) 194
Bse118I RCCGGY 2 cut(s) 78, 131
Bse1I ACTGG 2 cut(s) 509, 566
Bse3DI GCAATG 3 cut(s) 337, 453, 576
BseDI CCNNGG 1 cut(s) 11
BseGI GGATG 6 cut(s) 183, 253, 367, 381, 394, 406
BseLI CCNNNNNNNGG 1 cut(s) 194
BseMI GCAATG 3 cut(s) 337, 453, 576
BseMII CTCAG 1 cut(s) 386
BseNI ACTGG 2 cut(s) 509, 566
BseXI GCAGC 1 cut(s) 442
BsiHKAI GWGCWC 1 cut(s) 521
BsiSI CCGG 2 cut(s) 79, 132
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 1 cut(s) 194
BsmAI GTCTC 2 cut(s) 125, 203
BsmFI GGGAC 1 cut(s) 555
BsmI GAATGC 1 cut(s) 457
Bso31I GGTCTC 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 521
Bsp1407I TGTACA 1 cut(s) 150
Bsp143I GATC 4 cut(s) 3, 267, 340, 433
BspACI CCGC 2 cut(s) 129, 493
BspCNI CTCAG 1 cut(s) 387
BspMAI CTGCAG 1 cut(s) 460
BspOI GCTAGC 1 cut(s) 585
BspPI GGATC 2 cut(s) 262, 348
BspQI GCTCTTC 1 cut(s) 77
BspTNI GGTCTC 1 cut(s) 125
BsrDI GCAATG 3 cut(s) 337, 453, 576
BsrFI RCCGGY 2 cut(s) 78, 131
BsrGI TGTACA 1 cut(s) 150
BsrI ACTGG 2 cut(s) 509, 566
BssAI RCCGGY 2 cut(s) 78, 131
BssECI CCNNGG 1 cut(s) 11
BssMI GATC 4 cut(s) 3, 267, 340, 433
BssNI GRCGYC 1 cut(s) 126
Bst4CI ACNGT 4 cut(s) 15, 214, 241, 546
Bst6I CTCTTC 1 cut(s) 77
BstACI GRCGYC 1 cut(s) 126
BstAPI GCANNNNNTGC 1 cut(s) 452
BstAUI TGTACA 1 cut(s) 150
BstC8I GCNNGC 2 cut(s) 80, 583
BstDEI CTNAG 1 cut(s) 395
BstDSI CCRYGG 1 cut(s) 11
BstF5I GGATG 6 cut(s) 183, 253, 367, 381, 394, 406
BstKTI GATC 4 cut(s) 6, 270, 343, 436
BstMAI GTCTC 2 cut(s) 125, 203
BstMBI GATC 4 cut(s) 3, 267, 340, 433
BstMWI GCNNNNNNNGC 2 cut(s) 452, 587
BstNSI RCATGY 1 cut(s) 525
BstSFI CTRYAG 1 cut(s) 456
BstV1I GCAGC 1 cut(s) 442
BstV2I GAAGAC 1 cut(s) 473
BstX2I RGATCY 2 cut(s) 267, 433
BstYI RGATCY 2 cut(s) 267, 433
BtgI CCRYGG 1 cut(s) 11
BtsCI GGATG 6 cut(s) 183, 253, 367, 381, 394, 406
BtsIMutI CAGTG 2 cut(s) 237, 502
Cac8I GCNNGC 2 cut(s) 80, 583
Cfr10I RCCGGY 2 cut(s) 78, 131
CseI GACGC 1 cut(s) 134
Csp6I GTAC 3 cut(s) 151, 508, 547
CviAII CATG 1 cut(s) 522
CviQI GTAC 3 cut(s) 151, 508, 547
DdeI CTNAG 1 cut(s) 395
DpnI GATC 4 cut(s) 5, 269, 342, 435
DpnII GATC 4 cut(s) 3, 267, 340, 433
DraIII CACNNNGTG 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 77
EarI CTCTTC 1 cut(s) 77
EciI GGCGGA 1 cut(s) 482
Eco31I GGTCTC 1 cut(s) 125
Eco57I CTGAAG 1 cut(s) 58
EcoRI GAATTC 1 cut(s) 105
FaeI CATG 1 cut(s) 525
FaiI YATR 1 cut(s) 523
FaqI GGGAC 1 cut(s) 555
FatI CATG 1 cut(s) 521
Fnu4HI GCNGC 2 cut(s) 129, 456
FokI GGATG 6 cut(s) 170, 260, 374, 388, 401, 413
Fsp4HI GCNGC 2 cut(s) 129, 456
FspBI CTAG 1 cut(s) 582
GluI GCNGC 2 cut(s) 129, 456
GsuI CTGGAG 1 cut(s) 10
HapII CCGG 2 cut(s) 79, 132
HgaI GACGC 1 cut(s) 134
Hin1I GRCGYC 1 cut(s) 126
Hin1II CATG 1 cut(s) 525
HindIII AAGCTT 1 cut(s) 473
HpaII CCGG 2 cut(s) 79, 132
HphI GGTGA 2 cut(s) 169, 548
Hpy166II GTNNAC 1 cut(s) 63
Hpy188I TCNGA 4 cut(s) 111, 167, 197, 516
Hpy188III TCNNGA 4 cut(s) 64, 122, 260, 295
Hpy8I GTNNAC 1 cut(s) 63
Hpy99I CGWCG 1 cut(s) 128
HpyAV CCTTC 2 cut(s) 17, 30
HpyCH4III ACNGT 4 cut(s) 15, 214, 241, 546
HpyCH4IV ACGT 2 cut(s) 59, 499
HpyCH4V TGCA 4 cut(s) 370, 446, 458, 527
HpyF10VI GCNNNNNNNGC 2 cut(s) 452, 587
HpyF3I CTNAG 1 cut(s) 395
HpySE526I ACGT 2 cut(s) 59, 499
Hsp92I GRCGYC 1 cut(s) 126
Hsp92II CATG 1 cut(s) 525
KroI GCCGGC 1 cut(s) 78
KroNI GCCGGC 1 cut(s) 80
Kzo9I GATC 4 cut(s) 3, 267, 340, 433
LguI GCTCTTC 1 cut(s) 77
Lsp1109I GCAGC 1 cut(s) 442
LweI GCATC 2 cut(s) 90, 391
MaeI CTAG 1 cut(s) 582
MaeII ACGT 2 cut(s) 59, 499
MaeIII GTNAC 2 cut(s) 175, 500
MalI GATC 4 cut(s) 5, 269, 342, 435
MboI GATC 4 cut(s) 3, 267, 340, 433
MboII GAAGA 3 cut(s) 64, 428, 473
MflI RGATCY 2 cut(s) 267, 433
MhlI GDGCHC 1 cut(s) 521
MluCI AATT 1 cut(s) 105
MmeI TCCRAC 2 cut(s) 17, 594
MnlI CCTC 9 cut(s) 34, 276, 346, 351, 379, 390, 532, 541, 544
MroNI GCCGGC 1 cut(s) 78
MroXI GAANNNNTTC 1 cut(s) 71
MseI TTAA 2 cut(s) 201, 592
MspI CCGG 2 cut(s) 79, 132
Mva1269I GAATGC 1 cut(s) 457
MwoI GCNNNNNNNGC 2 cut(s) 452, 587
NaeI GCCGGC 1 cut(s) 80
NdeII GATC 4 cut(s) 3, 267, 340, 433
NgoMIV GCCGGC 1 cut(s) 78
NheI GCTAGC 1 cut(s) 581
NlaIII CATG 1 cut(s) 525
NmuCI GTSAC 2 cut(s) 175, 500
NspI RCATGY 1 cut(s) 525
PciI ACATGT 1 cut(s) 521
PciSI GCTCTTC 1 cut(s) 77
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PctI GAATGC 1 cut(s) 457
PdiI GCCGGC 1 cut(s) 80
PdmI GAANNNNTTC 1 cut(s) 71
PkrI GCNGC 2 cut(s) 130, 457
PscI ACATGT 1 cut(s) 521
PstI CTGCAG 1 cut(s) 460
PsuI RGATCY 2 cut(s) 267, 433
RsaI GTAC 3 cut(s) 152, 509, 548
RsaNI GTAC 3 cut(s) 151, 508, 547
SapI GCTCTTC 1 cut(s) 77
SaqAI TTAA 2 cut(s) 201, 592
SatI GCNGC 2 cut(s) 129, 456
Sau3AI GATC 4 cut(s) 3, 267, 340, 433
SduI GDGCHC 1 cut(s) 521
SetI ASST 9 cut(s) 62, 72, 159, 165, 236, 310, 477, 502, 533
SfaNI GCATC 2 cut(s) 90, 391
SfcI CTRYAG 1 cut(s) 456
SmlI CTYRAG 2 cut(s) 158, 303
SmoI CTYRAG 2 cut(s) 158, 303
Sse9I AATT 1 cut(s) 105
SsiI CCGC 2 cut(s) 129, 493
SspMI CTAG 1 cut(s) 582
TaaI ACNGT 4 cut(s) 15, 214, 241, 546
TaiI ACGT 2 cut(s) 62, 502
TaqI TCGA 2 cut(s) 123, 343
TaqII GACCGA 1 cut(s) 107
TasI AATT 1 cut(s) 105
TatI WGTACW 1 cut(s) 150
TauI GCSGC 1 cut(s) 131
Tru1I TTAA 2 cut(s) 201, 592
Tru9I TTAA 2 cut(s) 201, 592
TscAI CASTG 2 cut(s) 244, 509
TseFI GTSAC 2 cut(s) 175, 500
TseI GCWGC 1 cut(s) 455
Tsp45I GTSAC 2 cut(s) 175, 500
TspDTI ATGAA 3 cut(s) 405, 464, 473
TspGWI ACGGA 1 cut(s) 260
TspRI CASTG 2 cut(s) 244, 509
XapI RAATTY 1 cut(s) 105
XceI RCATGY 1 cut(s) 525
XmnI GAANNNNTTC 1 cut(s) 71
XspI CTAG 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.