pycom02g14440
ERF Family

Belongs to the small GTPase superfamily. Arf family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000844
Physical Location & Seq
Reverse (-)
11003924 .. 11005116
1193 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g14440.1

Sequence Viewer

Length: 495 bp
ATGAGAATACTAATGGTGGGTCTCGACGCTGCCGGAAAAACCACCATCCTCTACAAGCTCAAACTTGGAGAGGTTGTCACTACCATACCCACAATTGGGTTTAATGTGGAAACTGTTGAATACAAAAATGTGAGCTTCACTGTTTGGGATGTCGGAGGACAAGACAAGATTCGACCGCTATGGAGACATTATTTTCAGAACACTCAAGGTCTTATCTTTGTGCTGGATAGCAATGACAGAGACAGAGTTTTGGAAGCAAGAGATGAGCTCCATCGAATGCTCAGTGAGGATGAACTCCGTGACGCAACACTGCTTGTATTTGCCAACAAGCAAGATCTTCCGAATGCCATGAATGTCTCTGAGATCACTGATAAGCTTGGCCTGCATTCACTCCGGCAACGCAGATGGTACATTCAGGCTGCTAGTGCCACCTCCGGCCAAGGACTCTACGAGGGCCTCGATTGGCTATCCAGCAACTTCTCAAACAAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko05110 ko05134 map04072 map04144 map05110 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

165

Amino Acids

18.72

Weight (kDa)

5.91

Isoelectric Point (pI)

35.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 1 - 159 1.1e-75 ADP-ribosylation factor family
Roc PF08477 2 - 112 1.9e-13 Ras of Complex, Roc, domain of DAPkinase
SRPRB PF09439 2 - 126 1.7e-12 Signal recognition particle receptor beta subunit
Ras PF00071 2 - 122 2.9e-12 Ras family
Gtr1_RagA PF04670 2 - 122 2.7e-10 Gtr1/RagA G protein conserved region
G-alpha PF00503 28 - 94 1.8e-09 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 176
AcoI YGGCCR 1 cut(s) 436
AfaI GTAC 1 cut(s) 410
AfiI CCNNNNNNNGG 2 cut(s) 95, 96
AgsI TTSAA 1 cut(s) 119
AluBI AGCT 4 cut(s) 58, 135, 268, 376
AluI AGCT 4 cut(s) 58, 135, 268, 376
Alw21I GWGCWC 1 cut(s) 270
Alw26I GTCTC 4 cut(s) 26, 178, 234, 361
AoxI GGCC 3 cut(s) 379, 436, 454
ApeKI GCWGC 2 cut(s) 29, 419
AspS9I GGNCC 1 cut(s) 454
BanII GRGCYC 1 cut(s) 270
Bbv12I GWGCWC 1 cut(s) 270
BbvI GCAGC 2 cut(s) 16, 406
BccI CCATC 3 cut(s) 53, 279, 399
BcoDI GTCTC 4 cut(s) 26, 178, 234, 361
BfaI CTAG 1 cut(s) 423
BglII AGATCT 1 cut(s) 334
BisI GCNGC 2 cut(s) 30, 420
BlsI GCNGC 2 cut(s) 31, 421
BmgT120I GGNCC 1 cut(s) 454
BplI GAGNNNNNCTC 2 cut(s) 252, 284
BpuEI CTTGAG 1 cut(s) 189
BsaI GGTCTC 1 cut(s) 26
BsaJI CCNNGG 1 cut(s) 439
BsaXI ACNNNNNCTCC 2 cut(s) 60, 90
Bsc4I CCNNNNNNNGG 2 cut(s) 95, 96
Bse3DI GCAATG 1 cut(s) 238
BseDI CCNNGG 1 cut(s) 439
BseGI GGATG 3 cut(s) 45, 154, 295
BseLI CCNNNNNNNGG 2 cut(s) 95, 96
BseMI GCAATG 1 cut(s) 238
BseMII CTCAG 2 cut(s) 295, 351
BseXI GCAGC 2 cut(s) 16, 406
Bsh1285I CGRYCG 1 cut(s) 176
BshFI GGCC 3 cut(s) 381, 438, 456
BsiEI CGRYCG 1 cut(s) 176
BsiHKAI GWGCWC 1 cut(s) 270
BsiSI CCGG 3 cut(s) 33, 394, 435
BslI CCNNNNNNNGG 2 cut(s) 95, 96
BsmAI GTCTC 4 cut(s) 26, 178, 234, 361
BsmI GAATGC 3 cut(s) 282, 349, 385
BsnI GGCC 3 cut(s) 381, 438, 456
Bso31I GGTCTC 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 270
Bsp143I GATC 2 cut(s) 334, 363
BspACI CCGC 1 cut(s) 176
BspANI GGCC 3 cut(s) 381, 438, 456
BspCNI CTCAG 2 cut(s) 294, 352
BspTNI GGTCTC 1 cut(s) 26
BsrDI GCAATG 1 cut(s) 238
BssECI CCNNGG 1 cut(s) 439
BssMI GATC 2 cut(s) 334, 363
BssT1I CCWWGG 1 cut(s) 439
Bst4CI ACNGT 2 cut(s) 115, 142
BstC8I GCNNGC 1 cut(s) 383
BstDEI CTNAG 2 cut(s) 281, 360
BstF5I GGATG 3 cut(s) 45, 154, 295
BstKTI GATC 2 cut(s) 337, 366
BstMAI GTCTC 4 cut(s) 26, 178, 234, 361
BstMBI GATC 2 cut(s) 334, 363
BstMCI CGRYCG 1 cut(s) 176
BstMWI GCNNNNNNNGC 2 cut(s) 382, 425
BstV1I GCAGC 2 cut(s) 16, 406
BstX2I RGATCY 1 cut(s) 334
BstYI RGATCY 1 cut(s) 334
BsuRI GGCC 3 cut(s) 381, 438, 456
BtsCI GGATG 3 cut(s) 45, 154, 295
BtsI GCAGTG 1 cut(s) 308
BtsIMutI CAGTG 4 cut(s) 138, 289, 308, 366
Cac8I GCNNGC 1 cut(s) 383
Cfr13I GGNCC 1 cut(s) 454
CseI GACGC 2 cut(s) 35, 311
Csp6I GTAC 1 cut(s) 409
CviAII CATG 2 cut(s) 349, 492
CviQI GTAC 1 cut(s) 409
DdeI CTNAG 2 cut(s) 281, 360
DpnI GATC 2 cut(s) 336, 365
DpnII GATC 2 cut(s) 334, 363
EaeI YGGCCR 1 cut(s) 436
Ecl136II GAGCTC 1 cut(s) 268
Eco130I CCWWGG 1 cut(s) 439
Eco24I GRGCYC 1 cut(s) 270
Eco31I GGTCTC 1 cut(s) 26
Eco53kI GAGCTC 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 268
EcoO109I RGGNCCY 1 cut(s) 454
EcoT14I CCWWGG 1 cut(s) 439
EcoT38I GRGCYC 1 cut(s) 270
ErhI CCWWGG 1 cut(s) 439
FaeI CATG 2 cut(s) 352, 495
FaiI YATR 4 cut(s) 86, 181, 350, 493
FatI CATG 2 cut(s) 348, 491
Fnu4HI GCNGC 2 cut(s) 30, 420
FokI GGATG 3 cut(s) 32, 161, 302
FriOI GRGCYC 1 cut(s) 270
Fsp4HI GCNGC 2 cut(s) 30, 420
FspBI CTAG 1 cut(s) 423
GluI GCNGC 2 cut(s) 30, 420
HaeIII GGCC 3 cut(s) 381, 438, 456
HapII CCGG 3 cut(s) 33, 394, 435
HgaI GACGC 2 cut(s) 35, 311
Hin1II CATG 2 cut(s) 352, 495
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 2 cut(s) 169, 444
HpaII CCGG 3 cut(s) 33, 394, 435
Hpy188I TCNGA 4 cut(s) 155, 198, 342, 361
Hpy188III TCNNGA 1 cut(s) 23
Hpy99I CGWCG 1 cut(s) 29
HpyCH4III ACNGT 2 cut(s) 115, 142
HpyCH4V TGCA 1 cut(s) 385
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 425
HpyF3I CTNAG 2 cut(s) 281, 360
Hsp92II CATG 2 cut(s) 352, 495
Kzo9I GATC 2 cut(s) 334, 363
LmnI GCTCC 1 cut(s) 273
LpnPI CCDG 7 cut(s) 46, 209, 395, 401, 407, 448, 484
Lsp1109I GCAGC 2 cut(s) 16, 406
MaeI CTAG 1 cut(s) 423
MaeIII GTNAC 2 cut(s) 76, 299
MalI GATC 2 cut(s) 336, 365
MboI GATC 2 cut(s) 334, 363
MboII GAAGA 1 cut(s) 329
MfeI CAATTG 1 cut(s) 93
MflI RGATCY 1 cut(s) 334
MhlI GDGCHC 1 cut(s) 270
MluCI AATT 1 cut(s) 93
MlyI GAGTC 1 cut(s) 438
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 7 cut(s) 59, 64, 149, 280, 442, 445, 467
MseI TTAA 1 cut(s) 102
MspI CCGG 3 cut(s) 33, 394, 435
MunI CAATTG 1 cut(s) 93
Mva1269I GAATGC 3 cut(s) 282, 349, 385
MwoI GCNNNNNNNGC 2 cut(s) 382, 425
NdeII GATC 2 cut(s) 334, 363
NlaIII CATG 2 cut(s) 352, 495
NmuCI GTSAC 2 cut(s) 76, 299
PcsI WCGNNNNNNNCGW 1 cut(s) 456
PctI GAATGC 3 cut(s) 282, 349, 385
PfeI GAWTC 1 cut(s) 169
PkrI GCNGC 2 cut(s) 31, 421
PleI GAGTC 1 cut(s) 438
PpsI GAGTC 1 cut(s) 438
Psp124BI GAGCTC 1 cut(s) 270
PspPI GGNCC 1 cut(s) 454
PsuI RGATCY 1 cut(s) 334
RsaI GTAC 1 cut(s) 410
RsaNI GTAC 1 cut(s) 409
SacI GAGCTC 1 cut(s) 270
SaqAI TTAA 1 cut(s) 102
SatI GCNGC 2 cut(s) 30, 420
Sau3AI GATC 2 cut(s) 334, 363
Sau96I GGNCC 1 cut(s) 454
SchI GAGTC 1 cut(s) 438
SduI GDGCHC 1 cut(s) 270
SetI ASST 7 cut(s) 60, 75, 137, 211, 270, 378, 434
SmlI CTYRAG 1 cut(s) 204
SmoI CTYRAG 1 cut(s) 204
Sse9I AATT 1 cut(s) 93
SsiI CCGC 1 cut(s) 176
SspMI CTAG 1 cut(s) 423
SstI GAGCTC 1 cut(s) 270
StyI CCWWGG 1 cut(s) 439
TaaI ACNGT 2 cut(s) 115, 142
TaqI TCGA 4 cut(s) 24, 172, 274, 459
TasI AATT 1 cut(s) 93
TfiI GAWTC 1 cut(s) 169
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 4 cut(s) 145, 289, 315, 373
TseFI GTSAC 2 cut(s) 76, 299
TseI GCWGC 2 cut(s) 29, 419
Tsp45I GTSAC 2 cut(s) 76, 299
TspDTI ATGAA 2 cut(s) 306, 365
TspGWI ACGGA 1 cut(s) 287
TspRI CASTG 4 cut(s) 145, 289, 315, 373
XspI CTAG 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.