MD05G1087900.v1.1

ADP-ribosylation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000844
Physical Location & Seq
Reverse (-)
18824991 .. 18826810
1820 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1087900.v1.1.491

Sequence Viewer

Length: 549 bp
ATGGGAGGAGCGGTTTCGCGAATTGCAAAGAGGTTCTTCCCAAAGAATGGAGTGAGGATTCTAATGGTGGGTCTTGATGCTTCAGGAAAGACAACCATCCTCTACAAGCTGAAGCTTGGAGACATCGTTAACACCATACCCACCATCGGTTTCAATGTGGAGACAATAGAGTACAAAAATATTACCTTCACTATTTGGGATGTTGGTGGACAAGACAAGATACGGCCTTTGTGGAGGCACTACTTTCAGAATACTCAAGGCATTATATTTGTTGTGGACAGCATTGACAGGGAAAGAATCTCAGAAGCCCGCAACGAGCTTCATTGTATTTTAAGTGAGAGTGAACTAAGCAATGCAACACTTCTTGTCTTTGCTAATAAGCAAGACCTTCCACATGCTATGAGTTCTTCTGAGGTTGCTGATAAACTTGCCCTGCACTTACTCCGGAATCGTTTCTGGTACATCCAAAGTACTTCTGCCACCACTGGCCAAGGACTCTATGAAGGTCTTGATTGGCTATCTGATAATATTGTCAATAACAAGGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04072 ko04144 ko05110 ko05134 map04072 map04144 map05110 map05134
Pfam Domains
Protein Families
Arf

Protein Analysis

183

Amino Acids

20.52

Weight (kDa)

7.86

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 11 - 177 1.4e-75 ADP-ribosylation factor family
SRPRB PF09439 19 - 143 2.8e-14 Signal recognition particle receptor beta subunit
Ras PF00071 19 - 176 8e-14 Ras family
Roc PF08477 19 - 129 1.6e-13 Ras of Complex, Roc, domain of DAPkinase
Gtr1_RagA PF04670 19 - 143 3.6e-10 Gtr1/RagA G protein conserved region
G-alpha PF00503 37 - 130 8e-11 G-protein alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 47
AccBSI CCGCTC 1 cut(s) 11
AccII CGCG 1 cut(s) 19
AccIII TCCGGA 1 cut(s) 444
AciI CCGC 2 cut(s) 11, 310
AcoI YGGCCR 1 cut(s) 487
AcuI CTGAAG 2 cut(s) 66, 131
AfaI GTAC 3 cut(s) 173, 461, 472
AfiI CCNNNNNNNGG 2 cut(s) 47, 146
AgsI TTSAA 1 cut(s) 154
AluBI AGCT 3 cut(s) 109, 115, 319
AluI AGCT 3 cut(s) 109, 115, 319
Alw26I GTCTC 2 cut(s) 114, 155
Aor13HI TCCGGA 1 cut(s) 444
AoxI GGCC 2 cut(s) 224, 487
Asp700I GAANNNNTTC 1 cut(s) 452
BalI TGGCCA 1 cut(s) 489
BccI CCATC 2 cut(s) 104, 152
BceAI ACGGC 1 cut(s) 239
BcoDI GTCTC 2 cut(s) 114, 155
BmcAI AGTACT 1 cut(s) 472
BmsI GCATC 1 cut(s) 67
BpuEI CTTGAG 1 cut(s) 240
BsaJI CCNNGG 1 cut(s) 490
BsaWI WCCGGW 1 cut(s) 444
BsaXI ACNNNNNCTCC 3 cut(s) 27, 111, 141
Bsc4I CCNNNNNNNGG 2 cut(s) 47, 146
Bse1I ACTGG 1 cut(s) 490
Bse3DI GCAATG 1 cut(s) 358
BseAI TCCGGA 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 490
BseGI GGATG 3 cut(s) 96, 205, 462
BseLI CCNNNNNNNGG 2 cut(s) 47, 146
BseMI GCAATG 1 cut(s) 358
BseMII CTCAG 2 cut(s) 315, 402
BseNI ACTGG 1 cut(s) 490
BseRI GAGGAG 1 cut(s) 21
BsgI GTGCAG 1 cut(s) 419
Bsh1236I CGCG 1 cut(s) 19
BshFI GGCC 2 cut(s) 226, 489
BsiSI CCGG 1 cut(s) 445
BslI CCNNNNNNNGG 2 cut(s) 47, 146
BsmAI GTCTC 2 cut(s) 114, 155
BsnI GGCC 2 cut(s) 226, 489
Bsp13I TCCGGA 1 cut(s) 444
Bsp68I TCGCGA 1 cut(s) 19
BspACI CCGC 2 cut(s) 11, 310
BspANI GGCC 2 cut(s) 226, 489
BspCNI CTCAG 2 cut(s) 314, 403
BspEI TCCGGA 1 cut(s) 444
BspFNI CGCG 1 cut(s) 19
BsrBI CCGCTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 358
BsrI ACTGG 1 cut(s) 490
BssECI CCNNGG 1 cut(s) 490
BssT1I CCWWGG 1 cut(s) 490
BstC8I GCNNGC 1 cut(s) 310
BstDEI CTNAG 3 cut(s) 301, 347, 411
BstF5I GGATG 3 cut(s) 96, 205, 462
BstFNI CGCG 1 cut(s) 19
BstMAI GTCTC 2 cut(s) 114, 155
BstNSI RCATGY 1 cut(s) 398
BstUI CGCG 1 cut(s) 19
BsuRI GGCC 2 cut(s) 226, 489
BtsCI GGATG 3 cut(s) 96, 205, 462
BtsIMutI CAGTG 1 cut(s) 483
BtuMI TCGCGA 1 cut(s) 19
Cac8I GCNNGC 1 cut(s) 310
Csp6I GTAC 3 cut(s) 172, 460, 471
CviAII CATG 2 cut(s) 395, 546
CviJI RGCY 7 cut(s) 109, 115, 226, 308, 319, 489, 517
CviKI_1 RGCY 7 cut(s) 109, 115, 226, 308, 319, 489, 517
CviQI GTAC 3 cut(s) 172, 460, 471
DdeI CTNAG 3 cut(s) 301, 347, 411
EaeI YGGCCR 1 cut(s) 487
Eco130I CCWWGG 1 cut(s) 490
Eco57I CTGAAG 2 cut(s) 66, 131
EcoT14I CCWWGG 1 cut(s) 490
ErhI CCWWGG 1 cut(s) 490
FaeI CATG 2 cut(s) 398, 549
FaiI YATR 6 cut(s) 137, 266, 396, 401, 501, 547
FalI AAGNNNNNCTT 2 cut(s) 20, 52
FatI CATG 2 cut(s) 394, 545
FauI CCCGC 1 cut(s) 317
FokI GGATG 3 cut(s) 83, 212, 449
HaeIII GGCC 2 cut(s) 226, 489
HapII CCGG 1 cut(s) 445
Hin1II CATG 2 cut(s) 398, 549
HincII GTYRAC 1 cut(s) 130
HindII GTYRAC 1 cut(s) 130
HindIII AAGCTT 1 cut(s) 113
HinfI GANTC 4 cut(s) 58, 297, 448, 495
HpaI GTTAAC 1 cut(s) 130
HpaII CCGG 1 cut(s) 445
Hpy166II GTNNAC 4 cut(s) 130, 209, 277, 344
Hpy188I TCNGA 4 cut(s) 249, 304, 412, 523
Hpy188III TCNNGA 5 cut(s) 18, 74, 84, 445, 509
Hpy8I GTNNAC 4 cut(s) 130, 209, 277, 344
HpyAV CCTTC 3 cut(s) 196, 398, 497
HpyCH4V TGCA 3 cut(s) 26, 356, 436
HpyF3I CTNAG 3 cut(s) 301, 347, 411
Hsp92II CATG 2 cut(s) 398, 549
Kpn2I TCCGGA 1 cut(s) 444
KspAI GTTAAC 1 cut(s) 130
LmnI GCTCC 1 cut(s) 8
LpnPI CCDG 6 cut(s) 69, 274, 442, 446, 458, 471
LweI GCATC 1 cut(s) 67
MbiI CCGCTC 1 cut(s) 11
MboII GAAGA 2 cut(s) 28, 399
MlsI TGGCCA 1 cut(s) 489
MluCI AATT 1 cut(s) 21
MluNI TGGCCA 1 cut(s) 489
MlyI GAGTC 1 cut(s) 489
MnlI CCTC 5 cut(s) 24, 48, 110, 228, 406
Mox20I TGGCCA 1 cut(s) 489
MroI TCCGGA 1 cut(s) 444
MroXI GAANNNNTTC 1 cut(s) 452
MscI TGGCCA 1 cut(s) 489
MseI TTAA 2 cut(s) 129, 332
Msp20I TGGCCA 1 cut(s) 489
MspI CCGG 1 cut(s) 445
MvnI CGCG 1 cut(s) 19
NlaIII CATG 2 cut(s) 398, 549
NruI TCGCGA 1 cut(s) 19
NspI RCATGY 1 cut(s) 398
PdmI GAANNNNTTC 1 cut(s) 452
PfeI GAWTC 3 cut(s) 58, 297, 448
PflMI CCANNNNNTGG 1 cut(s) 47
PleI GAGTC 1 cut(s) 489
PpsI GAGTC 1 cut(s) 489
RruI TCGCGA 1 cut(s) 19
RsaI GTAC 3 cut(s) 173, 461, 472
RsaNI GTAC 3 cut(s) 172, 460, 471
SaqAI TTAA 2 cut(s) 129, 332
ScaI AGTACT 1 cut(s) 472
SchI GAGTC 1 cut(s) 489
SetI ASST 8 cut(s) 35, 111, 117, 188, 321, 390, 417, 508
SfaNI GCATC 1 cut(s) 67
SmlI CTYRAG 1 cut(s) 255
SmoI CTYRAG 1 cut(s) 255
Sse9I AATT 1 cut(s) 21
SsiI CCGC 2 cut(s) 11, 310
SspI AATATT 2 cut(s) 181, 529
StyI CCWWGG 1 cut(s) 490
TasI AATT 1 cut(s) 21
TatI WGTACW 2 cut(s) 171, 470
TfiI GAWTC 3 cut(s) 58, 297, 448
Tru1I TTAA 2 cut(s) 129, 332
Tru9I TTAA 2 cut(s) 129, 332
TscAI CASTG 1 cut(s) 490
TspDTI ATGAA 2 cut(s) 311, 516
TspRI CASTG 1 cut(s) 490
Van91I CCANNNNNTGG 1 cut(s) 47
XceI RCATGY 1 cut(s) 398
XmnI GAANNNNTTC 1 cut(s) 452
ZrmI AGTACT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.