AT5G39520

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003399
Physical Location & Seq
Reverse (-)
15823735 .. 15825566
1832 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G39520.1

Sequence Viewer

Length: 693 bp
ATGGCGGTAAGCTCTGCGGTGTATTCATGCCCGGGGATGATCGGAAGTTGTGGGCAGGTTAGATCAAAACGGTGGAGAGTGAAGTGTCACTTGTCACCTGTAAAATCCGCCAAGTATTCCTCTAAAATATCAACCGATATCGCTCTCCACGAGTCTCCTCAGGCCTTGTTTGATGAATATTTGGAAGACAAATCAAGGGTTTTCGAAGCAATGTTTCCGGATAAACCCAAAAACTATAGGCTTAATGAGGAAGAATGGAGAATCCAAATGTTACCAATAAAATTTTTCTTCCTCACTGCGTGTCCAGTGGTGGTCATGCGGATTAGATGCAAATCCAACGGTCAGGATTACCCATCAGACGTACCTCTACACATAACCAAAGTTCTTGAGCTCAACATGACGAAATGGGAGCTTCAAGGGCTTGACCGAGTAATGGAACCAACTGATTTCACTCTTGGAGTAAAAGGAGCATTATACCCAGACCGGCGTGGAAGGCACACGAGGCTCAAAGGTCGACTAGAAACGACCATTAGCTTCGTGCTTCCTTCAGTTCTCGCGCTTGTACCAGAAGATGTTAGGAGAAACATGGCCAATGCGATTTTGGCCGGTTTGGTGGATAACATGAAGCATAGGGTTATTGAGAGCTTGGTCGCTGATTATAGCAAATTCAAATATGAAAGAAAAAAACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

230

Amino Acids

26.44

Weight (kDa)

9.47

Isoelectric Point (pI)

50.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 57 - 223 1.2e-42 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 46
AccI GTMKAC 1 cut(s) 514
AccII CGCG 1 cut(s) 557
AccIII TCCGGA 1 cut(s) 217
AciI CCGC 4 cut(s) 5, 17, 108, 319
AcoI YGGCCR 2 cut(s) 588, 603
AcsI RAATTY 2 cut(s) 281, 665
AcuI CTGAAG 1 cut(s) 531
AdeI CACNNNGTG 1 cut(s) 300
AfaI GTAC 2 cut(s) 363, 564
AfiI CCNNNNNNNGG 1 cut(s) 433
AgsI TTSAA 2 cut(s) 416, 670
AluBI AGCT 5 cut(s) 12, 391, 412, 534, 645
AluI AGCT 5 cut(s) 12, 391, 412, 534, 645
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 1 cut(s) 159
Ama87I CYCGRG 1 cut(s) 31
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 3 cut(s) 162, 588, 603
ApoI RAATTY 2 cut(s) 281, 665
AspLEI GCGC 1 cut(s) 559
AsuC2I CCSGG 2 cut(s) 32, 33
AsuHPI GGTGA 1 cut(s) 87
AsuII TTCGAA 1 cut(s) 204
AvaI CYCGRG 1 cut(s) 31
AxyI CCTNAGG 1 cut(s) 159
BalI TGGCCA 1 cut(s) 590
BanII GRGCYC 1 cut(s) 393
BauI CACGAG 2 cut(s) 149, 499
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 393
BccI CCATC 1 cut(s) 361
BcnI CCSGG 2 cut(s) 32, 33
BcoDI GTCTC 1 cut(s) 159
BfaI CTAG 2 cut(s) 518, 691
BfmI CTRYAG 1 cut(s) 235
BfuAI ACCTGC 1 cut(s) 46
Bme1390I CCNGG 2 cut(s) 32, 33
BmeT110I CYCGRG 1 cut(s) 31
BmiI GGNNCC 1 cut(s) 438
BmrFI CCNGG 2 cut(s) 32, 33
BmsI GCATC 1 cut(s) 317
BpiI GAAGAC 1 cut(s) 192
Bpu14I TTCGAA 1 cut(s) 204
BpuEI CTTGAG 1 cut(s) 407
BpuMI CCSGG 2 cut(s) 32, 33
BsaBI GATNNNNATC 1 cut(s) 331
BsaJI CCNNGG 2 cut(s) 31, 32
BsaWI WCCGGW 1 cut(s) 217
Bsc4I CCNNNNNNNGG 1 cut(s) 433
Bse118I RCCGGY 2 cut(s) 483, 605
Bse1I ACTGG 1 cut(s) 305
Bse21I CCTNAGG 1 cut(s) 159
Bse3DI GCAATG 1 cut(s) 216
Bse8I GATNNNNATC 1 cut(s) 331
BseAI TCCGGA 1 cut(s) 217
BseDI CCNNGG 2 cut(s) 31, 32
BseGI GGATG 1 cut(s) 42
BseJI GATNNNNATC 1 cut(s) 331
BseLI CCNNNNNNNGG 1 cut(s) 433
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 1 cut(s) 173
BseNI ACTGG 1 cut(s) 305
BseRI GAGGAG 1 cut(s) 147
Bsh1236I CGCG 1 cut(s) 557
BshFI GGCC 3 cut(s) 164, 590, 605
BsiHKAI GWGCWC 1 cut(s) 393
BsiHKCI CYCGRG 1 cut(s) 31
BsiSI CCGG 4 cut(s) 32, 218, 484, 606
BslI CCNNNNNNNGG 1 cut(s) 433
BsmAI GTCTC 1 cut(s) 159
BsnI GGCC 3 cut(s) 164, 590, 605
BsoBI CYCGRG 1 cut(s) 31
Bsp119I TTCGAA 1 cut(s) 204
Bsp1286I GDGCHC 1 cut(s) 393
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 2 cut(s) 39, 62
BspACI CCGC 4 cut(s) 5, 17, 108, 319
BspANI GGCC 3 cut(s) 164, 590, 605
BspCNI CTCAG 1 cut(s) 172
BspEI TCCGGA 1 cut(s) 217
BspFNI CGCG 1 cut(s) 557
BspLI GGNNCC 1 cut(s) 438
BspMI ACCTGC 1 cut(s) 46
BspT104I TTCGAA 1 cut(s) 204
BsrDI GCAATG 1 cut(s) 216
BsrFI RCCGGY 2 cut(s) 483, 605
BsrI ACTGG 1 cut(s) 305
BssAI RCCGGY 2 cut(s) 483, 605
BssECI CCNNGG 2 cut(s) 31, 32
BssMI GATC 2 cut(s) 39, 62
BssSI CACGAG 2 cut(s) 149, 499
Bst2BI CACGAG 2 cut(s) 149, 499
Bst4CI ACNGT 2 cut(s) 72, 341
BstBI TTCGAA 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 159
BstF5I GGATG 1 cut(s) 42
BstFNI CGCG 1 cut(s) 557
BstHHI GCGC 1 cut(s) 559
BstKTI GATC 2 cut(s) 42, 65
BstMAI GTCTC 1 cut(s) 159
BstMBI GATC 2 cut(s) 39, 62
BstMWI GCNNNNNNNGC 4 cut(s) 418, 493, 502, 602
BstSCI CCNGG 2 cut(s) 30, 31
BstSFI CTRYAG 1 cut(s) 235
BstUI CGCG 1 cut(s) 557
BstV2I GAAGAC 1 cut(s) 192
Bsu36I CCTNAGG 1 cut(s) 159
BsuRI GGCC 3 cut(s) 164, 590, 605
BtsCI GGATG 1 cut(s) 42
BtsI GCAGTG 1 cut(s) 294
BtsIMutI CAGTG 2 cut(s) 294, 312
BveI ACCTGC 1 cut(s) 46
CfoI GCGC 1 cut(s) 559
Cfr10I RCCGGY 2 cut(s) 483, 605
Cfr9I CCCGGG 1 cut(s) 31
Csp6I GTAC 2 cut(s) 362, 563
CviAII CATG 5 cut(s) 27, 316, 397, 586, 622
CviQI GTAC 2 cut(s) 362, 563
DdeI CTNAG 1 cut(s) 159
DpnI GATC 2 cut(s) 41, 64
DpnII GATC 2 cut(s) 39, 62
DraIII CACNNNGTG 1 cut(s) 300
EaeI YGGCCR 2 cut(s) 588, 603
EciI GGCGGA 1 cut(s) 97
Ecl136II GAGCTC 1 cut(s) 391
Eco147I AGGCCT 1 cut(s) 164
Eco24I GRGCYC 1 cut(s) 393
Eco32I GATATC 1 cut(s) 139
Eco53kI GAGCTC 1 cut(s) 391
Eco57I CTGAAG 1 cut(s) 531
Eco81I CCTNAGG 1 cut(s) 159
Eco88I CYCGRG 1 cut(s) 31
EcoICRI GAGCTC 1 cut(s) 391
EcoRV GATATC 1 cut(s) 139
EcoT38I GRGCYC 1 cut(s) 393
FaeI CATG 5 cut(s) 30, 319, 400, 589, 625
FalI AAGNNNNNCTT 2 cut(s) 74, 106
FatI CATG 5 cut(s) 26, 315, 396, 585, 621
FblI GTMKAC 1 cut(s) 514
FokI GGATG 1 cut(s) 49
FriOI GRGCYC 1 cut(s) 393
FspBI CTAG 2 cut(s) 518, 691
GlaI GCGC 1 cut(s) 558
HaeIII GGCC 3 cut(s) 164, 590, 605
HapII CCGG 4 cut(s) 32, 218, 484, 606
HhaI GCGC 1 cut(s) 559
Hin1II CATG 5 cut(s) 30, 319, 400, 589, 625
Hin6I GCGC 1 cut(s) 557
HinP1I GCGC 1 cut(s) 557
HincII GTYRAC 1 cut(s) 515
HindII GTYRAC 1 cut(s) 515
HinfI GANTC 2 cut(s) 152, 261
HpaII CCGG 4 cut(s) 32, 218, 484, 606
HphI GGTGA 1 cut(s) 87
Hpy166II GTNNAC 1 cut(s) 515
Hpy188I TCNGA 2 cut(s) 44, 358
Hpy188III TCNNGA 3 cut(s) 218, 344, 386
Hpy8I GTNNAC 1 cut(s) 515
HpyAV CCTTC 2 cut(s) 486, 555
HpyCH4III ACNGT 2 cut(s) 72, 341
HpyCH4IV ACGT 1 cut(s) 360
HpyCH4V TGCA 1 cut(s) 330
HpyF10VI GCNNNNNNNGC 4 cut(s) 418, 493, 502, 602
HpyF3I CTNAG 1 cut(s) 159
HpySE526I ACGT 1 cut(s) 360
Hsp92II CATG 5 cut(s) 30, 319, 400, 589, 625
HspAI GCGC 1 cut(s) 557
Kpn2I TCCGGA 1 cut(s) 217
Kzo9I GATC 2 cut(s) 39, 62
LmnI GCTCC 2 cut(s) 409, 467
LweI GCATC 1 cut(s) 317
MaeI CTAG 2 cut(s) 518, 691
MaeII ACGT 1 cut(s) 360
MaeIII GTNAC 3 cut(s) 86, 93, 270
MalI GATC 2 cut(s) 41, 64
MboI GATC 2 cut(s) 39, 62
MboII GAAGA 4 cut(s) 197, 263, 280, 581
MhlI GDGCHC 1 cut(s) 393
MlsI TGGCCA 1 cut(s) 590
MluCI AATT 2 cut(s) 281, 665
MluNI TGGCCA 1 cut(s) 590
MlyI GAGTC 1 cut(s) 161
MmeI TCCRAC 1 cut(s) 360
MnlI CCTC 6 cut(s) 130, 168, 241, 302, 375, 495
Mox20I TGGCCA 1 cut(s) 590
MroI TCCGGA 1 cut(s) 217
MscI TGGCCA 1 cut(s) 590
MseI TTAA 1 cut(s) 243
Msp20I TGGCCA 1 cut(s) 590
MspI CCGG 4 cut(s) 32, 218, 484, 606
MspR9I CCNGG 2 cut(s) 32, 33
MvnI CGCG 1 cut(s) 557
MwoI GCNNNNNNNGC 4 cut(s) 418, 493, 502, 602
NciI CCSGG 2 cut(s) 32, 33
NdeII GATC 2 cut(s) 39, 62
NlaIII CATG 5 cut(s) 30, 319, 400, 589, 625
NlaIV GGNNCC 1 cut(s) 438
NmuCI GTSAC 2 cut(s) 86, 93
NspV TTCGAA 1 cut(s) 204
PceI AGGCCT 1 cut(s) 164
PcsI WCGNNNNNNNCGW 1 cut(s) 147
PfeI GAWTC 1 cut(s) 261
PleI GAGTC 1 cut(s) 160
PpsI GAGTC 1 cut(s) 160
Psp124BI GAGCTC 1 cut(s) 393
PspN4I GGNNCC 1 cut(s) 438
RsaI GTAC 2 cut(s) 363, 564
RsaNI GTAC 2 cut(s) 362, 563
SacI GAGCTC 1 cut(s) 393
SalI GTCGAC 1 cut(s) 513
SaqAI TTAA 1 cut(s) 243
Sau3AI GATC 2 cut(s) 39, 62
SchI GAGTC 1 cut(s) 161
ScrFI CCNGG 2 cut(s) 32, 33
SduI GDGCHC 1 cut(s) 393
SfaNI GCATC 1 cut(s) 317
SfcI CTRYAG 1 cut(s) 235
SfuI TTCGAA 1 cut(s) 204
SmaI CCCGGG 1 cut(s) 33
SmlI CTYRAG 1 cut(s) 386
SmoI CTYRAG 1 cut(s) 386
Sse9I AATT 2 cut(s) 281, 665
SseBI AGGCCT 1 cut(s) 164
SsiI CCGC 4 cut(s) 5, 17, 108, 319
SspI AATATT 1 cut(s) 179
SspMI CTAG 2 cut(s) 518, 691
SstI GAGCTC 1 cut(s) 393
StuI AGGCCT 1 cut(s) 164
StyD4I CCNGG 2 cut(s) 30, 31
TaaI ACNGT 2 cut(s) 72, 341
TaiI ACGT 1 cut(s) 363
TaqI TCGA 2 cut(s) 204, 514
TaqII GACCGA 1 cut(s) 441
TasI AATT 2 cut(s) 281, 665
TfiI GAWTC 1 cut(s) 261
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 2 cut(s) 301, 312
TseFI GTSAC 2 cut(s) 86, 93
Tsp45I GTSAC 2 cut(s) 86, 93
TspDTI ATGAA 4 cut(s) 15, 189, 638, 690
TspMI CCCGGG 1 cut(s) 31
TspRI CASTG 2 cut(s) 301, 312
XapI RAATTY 2 cut(s) 281, 665
XcmI CCANNNNNNNNNTGG 1 cut(s) 598
XmaI CCCGGG 1 cut(s) 31
XmiI GTMKAC 1 cut(s) 514
XspI CTAG 2 cut(s) 518, 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.