FvH4_6g48000

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003399
Physical Location & Seq
Reverse (-)
36244498 .. 36245704
1207 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g48000.t1

Sequence Viewer

Length: 756 bp
ATGGTTGAGGCAATTGCGATACGCCAACACTTGCTCTTTCTTGATAGAGGTATGCATTTTTCCTCGGAAAACCTGAAGCTGATGCTGAAGAAGAAGCAGCAAGAAAGGCATTGCCATTATGTAAAGGCGCTATCCAACTTGAAGCAGCAGCCGGAGAAGGATTACCAGAAGAAGCCTTTAACCTTGCACCACAGTATCAGTACTGGTGCCCCACTTTATGAGTCCTCCAAGGCTTCATTTGATCAATATATGGTGGATGAACAAAGGGTTTTTAAAGCCCTTTTTCCAGGCATAAAGGGAAGCAAAAAACTTAACAAGAAAGAGTGGAGGATTCAAATGCCAACCATGCAGTTCTTGTTCATGTCTGTCTGCCTTGTAATATACATCAATTTTAGATACAAATCCAAAGGAAAAGACTACCCTCCTCATGTTCCTCATCACATCTCCAATGTCATTGAGCTTGAAGCTACAAGGTGGGAACTTGAGGGCCTTCCCGATAAGTACCGGCCGCGTGACTTTAGCATATCTGTTAAAGGAACCATATATCCTGATAGACAGCAAGGAAAACAAAGCAGGCTTAGAACACAGCTTGACGTAATCGTAAGCTTCATTGTTTCACCTCTGCCTGGTTGGGTTCCACACAGTGTTATGCATAGCCTTACAGAATCGCTGGTGAAGACAGTTATGGCGGATTCGACATCCAATGGCAGATTGCTTGAAGACTATGATGAATTCAAAACGAAGAATGTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

252

Amino Acids

29.25

Weight (kDa)

9.56

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 80 - 245 4.5e-24 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 206
AccII CGCG 1 cut(s) 511
AciI CCGC 2 cut(s) 509, 689
AcoI YGGCCR 1 cut(s) 506
AcsI RAATTY 1 cut(s) 731
AcuI CTGAAG 2 cut(s) 95, 107
AdeI CACNNNGTG 1 cut(s) 644
AfaI GTAC 2 cut(s) 202, 503
AfiI CCNNNNNNNGG 1 cut(s) 626
AgsI TTSAA 5 cut(s) 142, 335, 464, 719, 736
AjnI CCWGG 2 cut(s) 286, 625
AloI GAACNNNNNNTCC 2 cut(s) 529, 561
AluBI AGCT 5 cut(s) 79, 460, 467, 589, 606
AluI AGCT 5 cut(s) 79, 460, 467, 589, 606
AoxI GGCC 2 cut(s) 487, 506
ApeKI GCWGC 3 cut(s) 97, 145, 148
ApoI RAATTY 1 cut(s) 731
AspLEI GCGC 1 cut(s) 130
AspS9I GGNCC 1 cut(s) 487
AsuHPI GGTGA 2 cut(s) 609, 685
BaeGI GKGCMC 1 cut(s) 211
BaeI ACNNNNGTAYC 2 cut(s) 178, 211
BanI GGYRCC 1 cut(s) 206
BbsI GAAGAC 2 cut(s) 683, 726
BbvI GCAGC 3 cut(s) 109, 157, 160
BciT130I CCWGG 2 cut(s) 288, 627
BclI TGATCA 1 cut(s) 241
BfoI RGCGCY 1 cut(s) 131
BisI GCNGC 4 cut(s) 98, 146, 149, 509
BlsI GCNGC 4 cut(s) 99, 147, 150, 510
BmcAI AGTACT 1 cut(s) 202
Bme1390I CCNGG 2 cut(s) 288, 627
BmgT120I GGNCC 1 cut(s) 487
BmiI GGNNCC 3 cut(s) 208, 538, 636
BmrFI CCNGG 2 cut(s) 288, 627
BmsI GCATC 1 cut(s) 72
BpiI GAAGAC 2 cut(s) 683, 726
BpuEI CTTGAG 1 cut(s) 503
BsaBI GATNNNNATC 1 cut(s) 400
BsaJI CCNNGG 2 cut(s) 63, 228
Bsc4I CCNNNNNNNGG 1 cut(s) 626
Bse118I RCCGGY 1 cut(s) 504
Bse1I ACTGG 1 cut(s) 208
Bse3DI GCAATG 1 cut(s) 109
Bse8I GATNNNNATC 1 cut(s) 400
BseBI CCWGG 2 cut(s) 288, 627
BseDI CCNNGG 2 cut(s) 63, 228
BseGI GGATG 2 cut(s) 262, 698
BseJI GATNNNNATC 1 cut(s) 400
BseLI CCNNNNNNNGG 1 cut(s) 626
BseMI GCAATG 1 cut(s) 109
BseNI ACTGG 1 cut(s) 208
BseRI GAGGAG 1 cut(s) 414
BseSI GKGCMC 1 cut(s) 211
BseX3I CGGCCG 1 cut(s) 506
BseXI GCAGC 3 cut(s) 109, 157, 160
Bsh1236I CGCG 1 cut(s) 511
Bsh1285I CGRYCG 1 cut(s) 509
BshFI GGCC 2 cut(s) 489, 508
BshNI GGYRCC 1 cut(s) 206
BsiEI CGRYCG 1 cut(s) 509
BsiSI CCGG 2 cut(s) 152, 505
BslI CCNNNNNNNGG 1 cut(s) 626
BsnI GGCC 2 cut(s) 489, 508
Bsp1286I GDGCHC 1 cut(s) 211
Bsp143I GATC 1 cut(s) 241
BspACI CCGC 2 cut(s) 509, 689
BspANI GGCC 2 cut(s) 489, 508
BspFNI CGCG 1 cut(s) 511
BspLI GGNNCC 3 cut(s) 208, 538, 636
BspT107I GGYRCC 1 cut(s) 206
BsrDI GCAATG 1 cut(s) 109
BsrFI RCCGGY 1 cut(s) 504
BsrI ACTGG 1 cut(s) 208
BssAI RCCGGY 1 cut(s) 504
BssECI CCNNGG 2 cut(s) 63, 228
BssMI GATC 1 cut(s) 241
BssT1I CCWWGG 1 cut(s) 228
Bst2UI CCWGG 2 cut(s) 288, 627
Bst4CI ACNGT 3 cut(s) 194, 644, 682
BstC8I GCNNGC 1 cut(s) 575
BstDEI CTNAG 1 cut(s) 578
BstF5I GGATG 2 cut(s) 262, 698
BstFNI CGCG 1 cut(s) 511
BstH2I RGCGCY 1 cut(s) 131
BstHHI GCGC 1 cut(s) 130
BstKTI GATC 1 cut(s) 244
BstMBI GATC 1 cut(s) 241
BstMCI CGRYCG 1 cut(s) 509
BstMWI GCNNNNNNNGC 2 cut(s) 106, 346
BstNI CCWGG 2 cut(s) 288, 627
BstSCI CCNGG 2 cut(s) 286, 625
BstSLI GKGCMC 1 cut(s) 211
BstUI CGCG 1 cut(s) 511
BstV1I GCAGC 3 cut(s) 109, 157, 160
BstV2I GAAGAC 2 cut(s) 683, 726
BstZI CGGCCG 1 cut(s) 506
BsuRI GGCC 2 cut(s) 489, 508
BtsCI GGATG 2 cut(s) 262, 698
BtsIMutI CAGTG 1 cut(s) 649
Cac8I GCNNGC 1 cut(s) 575
CfoI GCGC 1 cut(s) 130
Cfr10I RCCGGY 1 cut(s) 504
Cfr13I GGNCC 1 cut(s) 487
Csp6I GTAC 2 cut(s) 201, 502
CviAII CATG 3 cut(s) 346, 361, 428
CviQI GTAC 2 cut(s) 201, 502
DdeI CTNAG 1 cut(s) 578
DpnI GATC 1 cut(s) 243
DpnII GATC 1 cut(s) 241
DraI TTTAAA 1 cut(s) 274
DraIII CACNNNGTG 1 cut(s) 644
EaeI YGGCCR 1 cut(s) 506
EagI CGGCCG 1 cut(s) 506
EciI GGCGGA 1 cut(s) 704
EclXI CGGCCG 1 cut(s) 506
Eco130I CCWWGG 1 cut(s) 228
Eco52I CGGCCG 1 cut(s) 506
Eco57I CTGAAG 2 cut(s) 95, 107
EcoO109I RGGNCCY 1 cut(s) 487
EcoRI GAATTC 1 cut(s) 731
EcoRII CCWGG 2 cut(s) 286, 625
EcoT14I CCWWGG 1 cut(s) 228
EcoT22I ATGCAT 2 cut(s) 57, 654
ErhI CCWWGG 1 cut(s) 228
FaeI CATG 3 cut(s) 349, 364, 431
FatI CATG 3 cut(s) 345, 360, 427
FbaI TGATCA 1 cut(s) 241
Fnu4HI GCNGC 4 cut(s) 98, 146, 149, 509
FokI GGATG 2 cut(s) 269, 685
Fsp4HI GCNGC 4 cut(s) 98, 146, 149, 509
GlaI GCGC 1 cut(s) 129
GluI GCNGC 4 cut(s) 98, 146, 149, 509
HaeII RGCGCY 1 cut(s) 131
HaeIII GGCC 2 cut(s) 489, 508
HapII CCGG 2 cut(s) 152, 505
HhaI GCGC 1 cut(s) 130
Hin1II CATG 3 cut(s) 349, 364, 431
Hin6I GCGC 1 cut(s) 128
HinP1I GCGC 1 cut(s) 128
HindIII AAGCTT 1 cut(s) 604
HinfI GANTC 4 cut(s) 221, 331, 665, 692
HpaII CCGG 2 cut(s) 152, 505
HphI GGTGA 2 cut(s) 609, 685
Hpy188I TCNGA 1 cut(s) 67
Hpy188III TCNNGA 3 cut(s) 41, 494, 548
HpyAV CCTTC 2 cut(s) 151, 500
HpyCH4III ACNGT 3 cut(s) 194, 644, 682
HpyCH4IV ACGT 1 cut(s) 594
HpyCH4V TGCA 4 cut(s) 55, 187, 349, 652
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 346
HpyF3I CTNAG 1 cut(s) 578
HpySE526I ACGT 1 cut(s) 594
Hsp92II CATG 3 cut(s) 349, 364, 431
HspAI GCGC 1 cut(s) 128
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 1 cut(s) 241
Lsp1109I GCAGC 3 cut(s) 109, 157, 160
LweI GCATC 1 cut(s) 72
MaeII ACGT 1 cut(s) 594
MaeIII GTNAC 1 cut(s) 512
MalI GATC 1 cut(s) 243
MboI GATC 1 cut(s) 241
MboII GAAGA 6 cut(s) 100, 103, 181, 688, 731, 754
MfeI CAATTG 1 cut(s) 12
MhlI GDGCHC 1 cut(s) 211
MluCI AATT 3 cut(s) 12, 388, 731
MlyI GAGTC 1 cut(s) 230
MmeI TCCRAC 1 cut(s) 159
MnlI CCTC 9 cut(s) 41, 73, 235, 321, 432, 435, 444, 478, 630
Mph1103I ATGCAT 2 cut(s) 57, 654
MseI TTAA 4 cut(s) 179, 273, 312, 531
MspI CCGG 2 cut(s) 152, 505
MspR9I CCNGG 2 cut(s) 288, 627
MunI CAATTG 1 cut(s) 12
MvaI CCWGG 2 cut(s) 288, 627
MvnI CGCG 1 cut(s) 511
MwoI GCNNNNNNNGC 2 cut(s) 106, 346
NdeII GATC 1 cut(s) 241
NlaIII CATG 3 cut(s) 349, 364, 431
NlaIV GGNNCC 3 cut(s) 208, 538, 636
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 2 cut(s) 57, 654
PfeI GAWTC 3 cut(s) 331, 665, 692
PkrI GCNGC 4 cut(s) 99, 147, 150, 510
PleI GAGTC 1 cut(s) 229
PpsI GAGTC 1 cut(s) 229
Psp6I CCWGG 2 cut(s) 286, 625
PspGI CCWGG 2 cut(s) 286, 625
PspN4I GGNNCC 3 cut(s) 208, 538, 636
PspPI GGNCC 1 cut(s) 487
RsaI GTAC 2 cut(s) 202, 503
RsaNI GTAC 2 cut(s) 201, 502
SaqAI TTAA 4 cut(s) 179, 273, 312, 531
SatI GCNGC 4 cut(s) 98, 146, 149, 509
Sau3AI GATC 1 cut(s) 241
Sau96I GGNCC 1 cut(s) 487
ScaI AGTACT 1 cut(s) 202
SchI GAGTC 1 cut(s) 230
ScrFI CCNGG 2 cut(s) 288, 627
SduI GDGCHC 1 cut(s) 211
SfaNI GCATC 1 cut(s) 72
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 3 cut(s) 12, 388, 731
SsiI CCGC 2 cut(s) 509, 689
StyD4I CCNGG 2 cut(s) 286, 625
StyI CCWWGG 1 cut(s) 228
TaaI ACNGT 3 cut(s) 194, 644, 682
TaiI ACGT 1 cut(s) 597
TaqI TCGA 1 cut(s) 695
TasI AATT 3 cut(s) 12, 388, 731
TatI WGTACW 1 cut(s) 200
TauI GCSGC 1 cut(s) 511
TfiI GAWTC 3 cut(s) 331, 665, 692
Tru1I TTAA 4 cut(s) 179, 273, 312, 531
Tru9I TTAA 4 cut(s) 179, 273, 312, 531
TscAI CASTG 1 cut(s) 649
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 3 cut(s) 97, 145, 148
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 5 cut(s) 225, 273, 349, 598, 744
TspRI CASTG 1 cut(s) 649
XapI RAATTY 1 cut(s) 731
ZrmI AGTACT 1 cut(s) 202
Zsp2I ATGCAT 2 cut(s) 57, 654
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.