FvH4_6g48001

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003399
Physical Location & Seq
Reverse (-)
36246110 .. 36247428
1319 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g48001.t1

Sequence Viewer

Length: 753 bp
ATGAAAATGGTCGAGGCAGTTACTTCATGCCAACCCTTGTTCGTTCTTGGTAGTGGTATGCGCTTTCGGCCAGAAAAATTGAAGGTGAAGCTGAAGAAGGACAACCGTTGCCATTATGCGAAAGCGGTATTGAATTCCAAACAGCAGCCGAAAAAGGATCATAAGAAGCCTCTAACTGTGAACCTCAGTACCACTATTGATGCACATCTGTATGAGTCTCCTGAGGTTTCATTTGATCAATATATGGAGGATCAACAAAGGGTTGTTAAAGCTATTTTTCCAGGCATAAAAGATCAGCAACTTAAAAAGAAAGAGTGGAGGATTCAAATGCCAACCATGCAATTCTTGTTCCTGTCTATCTACTTTGCATTCTACATCAAGTTAAGATACAAATCCGTGGGAAAAGACTACCCTCCTCATGTTCCTCATCACATTCCCAAGATCATTGAGCTTGAACTTACAAGATGGGAACTTGGGGGGATATCTGAGGAGTACCTGCCACGTGACTTAAGCGTCTCCCTCAAAGGAACCATATATCCTGATAGAGAGCAAGGAACACAAAGCAGGCTTAGAAGCCACTTTGACATGAACTTGAGCTTCATTCCATCACCTCTACTTGGTTGGCTTCCTCAGAGTGTTATGCAGGGCCTAATAGAAGCGCTGGTTAAGAAAGCCATGGTGGATTCGACATCAAATGCCAGATTGCTTGAAGACTATAACGAATTCAAGGCGGAGAAACTCAAGAAAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

251

Amino Acids

29.1

Weight (kDa)

9.52

Isoelectric Point (pI)

48.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 78 - 242 1.8e-22 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 512
Acc36I ACCTGC 1 cut(s) 504
AciI CCGC 2 cut(s) 125, 731
AclWI GGATC 2 cut(s) 165, 258
AcoI YGGCCR 1 cut(s) 68
AcsI RAATTY 2 cut(s) 133, 722
AcuI CTGAAG 1 cut(s) 113
AcvI CACGTG 1 cut(s) 503
AfaI GTAC 2 cut(s) 190, 494
AfeI AGCGCT 1 cut(s) 660
AfiI CCNNNNNNNGG 1 cut(s) 617
AflII CTTAAG 1 cut(s) 508
AgsI TTSAA 6 cut(s) 82, 133, 326, 455, 710, 727
AjnI CCWGG 1 cut(s) 280
AloI GAACNNNNNNTCC 2 cut(s) 520, 552
AluBI AGCT 4 cut(s) 91, 272, 451, 597
AluI AGCT 4 cut(s) 91, 272, 451, 597
Alw26I GTCTC 2 cut(s) 222, 520
AlwI GGATC 2 cut(s) 165, 258
Aor51HI AGCGCT 1 cut(s) 660
AoxI GGCC 2 cut(s) 68, 646
ApeKI GCWGC 1 cut(s) 145
ApoI RAATTY 2 cut(s) 133, 722
AspLEI GCGC 2 cut(s) 63, 661
AspS9I GGNCC 1 cut(s) 646
AsuHPI GGTGA 2 cut(s) 97, 600
AxyI CCTNAGG 1 cut(s) 222
BaeI ACNNNNGTAYC 2 cut(s) 172, 205
BbrPI CACGTG 1 cut(s) 503
BbsI GAAGAC 1 cut(s) 717
BbvI GCAGC 1 cut(s) 157
BccI CCATC 2 cut(s) 459, 613
BciT130I CCWGG 1 cut(s) 282
BclI TGATCA 1 cut(s) 235
BcoDI GTCTC 2 cut(s) 222, 520
BfoI RGCGCY 1 cut(s) 662
BfrI CTTAAG 1 cut(s) 508
BfuAI ACCTGC 1 cut(s) 504
BisI GCNGC 1 cut(s) 146
BlsI GCNGC 1 cut(s) 147
Bme1390I CCNGG 1 cut(s) 282
BmgT120I GGNCC 1 cut(s) 646
BmiI GGNNCC 1 cut(s) 529
BmrFI CCNGG 1 cut(s) 282
BmsI GCATC 1 cut(s) 190
BpiI GAAGAC 1 cut(s) 717
BpuEI CTTGAG 2 cut(s) 613, 725
BsaAI YACGTR 1 cut(s) 503
BsaBI GATNNNNATC 2 cut(s) 204, 391
BsaJI CCNNGG 2 cut(s) 396, 675
Bsc4I CCNNNNNNNGG 1 cut(s) 617
Bse21I CCTNAGG 1 cut(s) 222
Bse8I GATNNNNATC 2 cut(s) 204, 391
BseBI CCWGG 1 cut(s) 282
BseDI CCNNGG 2 cut(s) 396, 675
BseJI GATNNNNATC 2 cut(s) 204, 391
BseLI CCNNNNNNNGG 1 cut(s) 617
BseMII CTCAG 4 cut(s) 199, 213, 477, 644
BseRI GAGGAG 2 cut(s) 405, 503
BseXI GCAGC 1 cut(s) 157
BshFI GGCC 2 cut(s) 70, 648
BslI CCNNNNNNNGG 1 cut(s) 617
BsmAI GTCTC 2 cut(s) 222, 520
BsmBI CGTCTC 1 cut(s) 520
BsmI GAATGC 1 cut(s) 368
BsnI GGCC 2 cut(s) 70, 648
Bsp143I GATC 5 cut(s) 157, 235, 250, 292, 441
Bsp19I CCATGG 1 cut(s) 675
BspACI CCGC 2 cut(s) 125, 731
BspANI GGCC 2 cut(s) 70, 648
BspCNI CTCAG 4 cut(s) 198, 214, 478, 643
BspLI GGNNCC 1 cut(s) 529
BspMI ACCTGC 1 cut(s) 504
BspPI GGATC 2 cut(s) 165, 258
BspTI CTTAAG 1 cut(s) 508
BssECI CCNNGG 2 cut(s) 396, 675
BssMI GATC 5 cut(s) 157, 235, 250, 292, 441
BssT1I CCWWGG 1 cut(s) 675
Bst2UI CCWGG 1 cut(s) 282
Bst4CI ACNGT 2 cut(s) 107, 178
BstAFI CTTAAG 1 cut(s) 508
BstBAI YACGTR 1 cut(s) 503
BstC8I GCNNGC 1 cut(s) 566
BstDEI CTNAG 5 cut(s) 185, 222, 486, 569, 630
BstDSI CCRYGG 2 cut(s) 396, 675
BstH2I RGCGCY 1 cut(s) 662
BstHHI GCGC 2 cut(s) 63, 661
BstKTI GATC 5 cut(s) 160, 238, 253, 295, 444
BstMAI GTCTC 2 cut(s) 222, 520
BstMBI GATC 5 cut(s) 157, 235, 250, 292, 441
BstMWI GCNNNNNNNGC 2 cut(s) 67, 337
BstNI CCWGG 1 cut(s) 282
BstSCI CCNGG 1 cut(s) 280
BstV1I GCAGC 1 cut(s) 157
BstV2I GAAGAC 1 cut(s) 717
Bsu36I CCTNAGG 1 cut(s) 222
BsuRI GGCC 2 cut(s) 70, 648
BtgI CCRYGG 2 cut(s) 396, 675
BveI ACCTGC 1 cut(s) 504
Cac8I GCNNGC 1 cut(s) 566
CfoI GCGC 2 cut(s) 63, 661
Cfr13I GGNCC 1 cut(s) 646
CseI GACGC 1 cut(s) 502
Csp6I GTAC 2 cut(s) 189, 493
CviAII CATG 5 cut(s) 27, 337, 419, 586, 676
CviQI GTAC 2 cut(s) 189, 493
DdeI CTNAG 5 cut(s) 185, 222, 486, 569, 630
DpnI GATC 5 cut(s) 159, 237, 252, 294, 443
DpnII GATC 5 cut(s) 157, 235, 250, 292, 441
DrdI GACNNNNNNGTC 1 cut(s) 512
DseDI GACNNNNNNGTC 1 cut(s) 512
EaeI YGGCCR 1 cut(s) 68
EciI GGCGGA 1 cut(s) 746
Eco130I CCWWGG 1 cut(s) 675
Eco32I GATATC 1 cut(s) 483
Eco47III AGCGCT 1 cut(s) 660
Eco57I CTGAAG 1 cut(s) 113
Eco72I CACGTG 1 cut(s) 503
Eco81I CCTNAGG 1 cut(s) 222
EcoO109I RGGNCCY 1 cut(s) 646
EcoRI GAATTC 2 cut(s) 133, 722
EcoRII CCWGG 1 cut(s) 280
EcoRV GATATC 1 cut(s) 483
EcoT14I CCWWGG 1 cut(s) 675
ErhI CCWWGG 1 cut(s) 675
Esp3I CGTCTC 1 cut(s) 520
FaeI CATG 5 cut(s) 30, 340, 422, 589, 679
FatI CATG 5 cut(s) 26, 336, 418, 585, 675
FbaI TGATCA 1 cut(s) 235
Fnu4HI GCNGC 1 cut(s) 146
Fsp4HI GCNGC 1 cut(s) 146
GlaI GCGC 2 cut(s) 62, 660
GluI GCNGC 1 cut(s) 146
HaeII RGCGCY 1 cut(s) 662
HaeIII GGCC 2 cut(s) 70, 648
HgaI GACGC 1 cut(s) 502
HhaI GCGC 2 cut(s) 63, 661
Hin1II CATG 5 cut(s) 30, 340, 422, 589, 679
Hin6I GCGC 2 cut(s) 61, 659
HinP1I GCGC 2 cut(s) 61, 659
HinfI GANTC 3 cut(s) 215, 322, 683
HphI GGTGA 2 cut(s) 97, 600
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 2 cut(s) 487, 633
Hpy188III TCNNGA 3 cut(s) 221, 539, 742
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 2 cut(s) 76, 91
HpyCH4III ACNGT 2 cut(s) 107, 178
HpyCH4IV ACGT 1 cut(s) 502
HpyCH4V TGCA 4 cut(s) 203, 340, 368, 643
HpyF10VI GCNNNNNNNGC 2 cut(s) 67, 337
HpyF3I CTNAG 5 cut(s) 185, 222, 486, 569, 630
HpySE526I ACGT 1 cut(s) 502
Hsp92II CATG 5 cut(s) 30, 340, 422, 589, 679
HspAI GCGC 2 cut(s) 61, 659
Ksp22I TGATCA 1 cut(s) 235
Kzo9I GATC 5 cut(s) 157, 235, 250, 292, 441
Lsp1109I GCAGC 1 cut(s) 157
LweI GCATC 1 cut(s) 190
MaeII ACGT 1 cut(s) 502
MaeIII GTNAC 2 cut(s) 19, 503
MalI GATC 5 cut(s) 159, 237, 252, 294, 443
MboI GATC 5 cut(s) 157, 235, 250, 292, 441
MboII GAAGA 2 cut(s) 106, 722
MluCI AATT 4 cut(s) 77, 133, 341, 722
MlyI GAGTC 1 cut(s) 224
MseI TTAA 5 cut(s) 267, 303, 383, 509, 666
MslI CAYNNNNRTG 1 cut(s) 210
MspCI CTTAAG 1 cut(s) 508
MspR9I CCNGG 1 cut(s) 282
Mva1269I GAATGC 1 cut(s) 368
MvaI CCWGG 1 cut(s) 282
MwoI GCNNNNNNNGC 2 cut(s) 67, 337
NcoI CCATGG 1 cut(s) 675
NdeII GATC 5 cut(s) 157, 235, 250, 292, 441
NlaIII CATG 5 cut(s) 30, 340, 422, 589, 679
NlaIV GGNNCC 1 cut(s) 529
NmuCI GTSAC 1 cut(s) 503
PctI GAATGC 1 cut(s) 368
PfeI GAWTC 2 cut(s) 322, 683
PkrI GCNGC 1 cut(s) 147
PleI GAGTC 1 cut(s) 223
PmaCI CACGTG 1 cut(s) 503
PmlI CACGTG 1 cut(s) 503
PpsI GAGTC 1 cut(s) 223
Ppu21I YACGTR 1 cut(s) 503
Psp6I CCWGG 1 cut(s) 280
PspCI CACGTG 1 cut(s) 503
PspGI CCWGG 1 cut(s) 280
PspN4I GGNNCC 1 cut(s) 529
PspPI GGNCC 1 cut(s) 646
RsaI GTAC 2 cut(s) 190, 494
RsaNI GTAC 2 cut(s) 189, 493
RseI CAYNNNNRTG 1 cut(s) 210
SaqAI TTAA 5 cut(s) 267, 303, 383, 509, 666
SatI GCNGC 1 cut(s) 146
Sau3AI GATC 5 cut(s) 157, 235, 250, 292, 441
Sau96I GGNCC 1 cut(s) 646
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 282
SfaNI GCATC 1 cut(s) 190
SmiMI CAYNNNNRTG 1 cut(s) 210
SmlI CTYRAG 3 cut(s) 508, 592, 740
SmoI CTYRAG 3 cut(s) 508, 592, 740
Sse9I AATT 4 cut(s) 77, 133, 341, 722
SsiI CCGC 2 cut(s) 125, 731
StyD4I CCNGG 1 cut(s) 280
StyI CCWWGG 1 cut(s) 675
TaaI ACNGT 2 cut(s) 107, 178
TaiI ACGT 1 cut(s) 505
TaqI TCGA 2 cut(s) 12, 686
TasI AATT 4 cut(s) 77, 133, 341, 722
TfiI GAWTC 2 cut(s) 322, 683
Tru1I TTAA 5 cut(s) 267, 303, 383, 509, 666
Tru9I TTAA 5 cut(s) 267, 303, 383, 509, 666
TseFI GTSAC 1 cut(s) 503
TseI GCWGC 1 cut(s) 145
Tsp45I GTSAC 1 cut(s) 503
TspDTI ATGAA 5 cut(s) 15, 17, 219, 589, 602
TspGWI ACGGA 1 cut(s) 385
Vha464I CTTAAG 1 cut(s) 508
XapI RAATTY 2 cut(s) 133, 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.