MD09G1055400.v1.1

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003399
Physical Location & Seq
Forward (+)
3680678 .. 3681978
1301 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1055400.v1.1.491

Sequence Viewer

Length: 570 bp
ATGGGTGAGTTTGCTGCTGCTGCTGGTCTTTTTTCTGTAGGGCACTGCCAACCCTTGTTCTTTCCTGGTAGCGGAATGTGTTTCGGGCCGGAAAATATAGTGTGTAAGCCGATCAGGAAGCAGGAGAGTATTTGCCGTGCCAAAGCGGTGTTAAAATCGAAGAAAACGATGCAGCATAGGGAGCCTTTAAACTTTTACTTTAGATATACATTTGATGCTCCTCTCAATGAGTCACCTGCAGAAGAGTGGAGAATTCACATGCCGGCCATGCAAGTCTTGTTCCTGTCTGTCTACCTTGCATTATATGTAAAGTTGAGATGCAAATCCATGGGGAAAGACTACCCTCCTCATGTTCCTCACCACATCTCCAAGATTCTTGAGCTTGACGTTACTAGATGGGAAATCCGAGGCATTGCTGGGTATGATCCGAGTGGCGTCAACCTCAGCGTCAAAGGAATCATATTTTCAGATAGACAGGGAACACAAAGCTGGTTCAGAAACATATTCGATATAAACTTAAGCTTCATCGTACCACCTCTCTTCGCTTGGGTCCCGGACAATGTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

190

Amino Acids

21.55

Weight (kDa)

8.99

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 80 - 188 5.5e-11 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 244
Acc36I ACCTGC 1 cut(s) 244
AccI GTMKAC 1 cut(s) 291
AciI CCGC 2 cut(s) 72, 146
AclWI GGATC 1 cut(s) 419
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 1 cut(s) 252
AcyI GRCGYC 1 cut(s) 435
AfaI GTAC 1 cut(s) 531
AfiI CCNNNNNNNGG 1 cut(s) 71
AflII CTTAAG 1 cut(s) 517
AjnI CCWGG 1 cut(s) 64
AluBI AGCT 3 cut(s) 382, 489, 522
AluI AGCT 3 cut(s) 382, 489, 522
AlwI GGATC 1 cut(s) 419
AoxI GGCC 2 cut(s) 86, 264
ApeKI GCWGC 4 cut(s) 14, 17, 20, 172
ApoI RAATTY 1 cut(s) 252
AspS9I GGNCC 2 cut(s) 86, 550
AsuC2I CCSGG 1 cut(s) 554
AsuHPI GGTGA 3 cut(s) 17, 225, 350
AvaII GGWCC 1 cut(s) 550
BaeGI GKGCMC 1 cut(s) 45
BbvCI CCTCAGC 1 cut(s) 443
BbvI GCAGC 3 cut(s) 4, 7, 184
BccI CCATC 1 cut(s) 390
BceAI ACGGC 1 cut(s) 120
BciT130I CCWGG 1 cut(s) 66
BcnI CCSGG 1 cut(s) 554
BfaI CTAG 1 cut(s) 393
BfmI CTRYAG 2 cut(s) 36, 237
BfrI CTTAAG 1 cut(s) 517
BfuAI ACCTGC 1 cut(s) 244
BisI GCNGC 4 cut(s) 15, 18, 21, 173
BlsI GCNGC 4 cut(s) 16, 19, 22, 174
Bme1390I CCNGG 2 cut(s) 66, 554
Bme18I GGWCC 1 cut(s) 550
BmgT120I GGNCC 2 cut(s) 86, 550
BmiI GGNNCC 3 cut(s) 183, 551, 552
BmrFI CCNGG 2 cut(s) 66, 554
BmsI GCATC 3 cut(s) 159, 205, 308
Bpu10I CCTNAGC 1 cut(s) 443
BpuEI CTTGAG 1 cut(s) 398
BpuMI CCSGG 1 cut(s) 554
BsaBI GATNNNNATC 1 cut(s) 322
BsaHI GRCGYC 1 cut(s) 435
BsaJI CCNNGG 2 cut(s) 327, 406
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 1 cut(s) 71
Bse118I RCCGGY 1 cut(s) 262
Bse3DI GCAATG 1 cut(s) 411
Bse8I GATNNNNATC 1 cut(s) 322
BseBI CCWGG 1 cut(s) 66
BseDI CCNNGG 2 cut(s) 327, 406
BseJI GATNNNNATC 1 cut(s) 322
BseLI CCNNNNNNNGG 1 cut(s) 71
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 1 cut(s) 457
BseRI GAGGAG 2 cut(s) 210, 336
BseSI GKGCMC 1 cut(s) 45
BseXI GCAGC 3 cut(s) 4, 7, 184
BseYI CCCAGC 1 cut(s) 416
BshFI GGCC 2 cut(s) 88, 266
BsiSI CCGG 3 cut(s) 89, 263, 554
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 1 cut(s) 71
BsmFI GGGAC 1 cut(s) 536
BsnI GGCC 2 cut(s) 88, 266
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 2 cut(s) 111, 424
Bsp19I CCATGG 1 cut(s) 327
BspACI CCGC 2 cut(s) 72, 146
BspANI GGCC 2 cut(s) 88, 266
BspCNI CTCAG 1 cut(s) 456
BspLI GGNNCC 3 cut(s) 183, 551, 552
BspMAI CTGCAG 1 cut(s) 241
BspMI ACCTGC 1 cut(s) 244
BspPI GGATC 1 cut(s) 419
BspTI CTTAAG 1 cut(s) 517
BsrDI GCAATG 1 cut(s) 411
BsrFI RCCGGY 1 cut(s) 262
BssAI RCCGGY 1 cut(s) 262
BssECI CCNNGG 2 cut(s) 327, 406
BssMI GATC 2 cut(s) 111, 424
BssNI GRCGYC 1 cut(s) 435
BssT1I CCWWGG 1 cut(s) 327
Bst2UI CCWGG 1 cut(s) 66
Bst6I CTCTTC 2 cut(s) 237, 545
BstACI GRCGYC 1 cut(s) 435
BstAFI CTTAAG 1 cut(s) 517
BstC8I GCNNGC 1 cut(s) 264
BstDEI CTNAG 1 cut(s) 443
BstDSI CCRYGG 1 cut(s) 327
BstKTI GATC 2 cut(s) 114, 427
BstMBI GATC 2 cut(s) 111, 424
BstMWI GCNNNNNNNGC 3 cut(s) 20, 181, 268
BstNI CCWGG 1 cut(s) 66
BstNSI RCATGY 1 cut(s) 262
BstSCI CCNGG 2 cut(s) 64, 552
BstSFI CTRYAG 2 cut(s) 36, 237
BstSLI GKGCMC 1 cut(s) 45
BstV1I GCAGC 3 cut(s) 4, 7, 184
BsuRI GGCC 2 cut(s) 88, 266
BtgI CCRYGG 1 cut(s) 327
BtsI GCAGTG 1 cut(s) 43
BtsIMutI CAGTG 1 cut(s) 43
BveI ACCTGC 1 cut(s) 244
Cac8I GCNNGC 1 cut(s) 264
Cfr10I RCCGGY 1 cut(s) 262
Cfr13I GGNCC 2 cut(s) 86, 550
CseI GACGC 2 cut(s) 424, 436
Csp6I GTAC 1 cut(s) 530
CviAII CATG 5 cut(s) 259, 268, 328, 350, 565
CviJI RGCY 7 cut(s) 88, 109, 184, 266, 382, 489, 522
CviKI_1 RGCY 7 cut(s) 88, 109, 184, 266, 382, 489, 522
CviQI GTAC 1 cut(s) 530
DdeI CTNAG 1 cut(s) 443
DpnI GATC 2 cut(s) 113, 426
DpnII GATC 2 cut(s) 111, 424
DraI TTTAAA 1 cut(s) 189
EaeI YGGCCR 1 cut(s) 264
Eam1104I CTCTTC 2 cut(s) 237, 545
EarI CTCTTC 2 cut(s) 237, 545
Eco130I CCWWGG 1 cut(s) 327
Eco47I GGWCC 1 cut(s) 550
EcoO109I RGGNCCY 1 cut(s) 550
EcoRI GAATTC 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 64
EcoT14I CCWWGG 1 cut(s) 327
ErhI CCWWGG 1 cut(s) 327
FaeI CATG 5 cut(s) 262, 271, 331, 353, 568
FaqI GGGAC 1 cut(s) 536
FatI CATG 5 cut(s) 258, 267, 327, 349, 564
FblI GTMKAC 1 cut(s) 291
Fnu4HI GCNGC 4 cut(s) 15, 18, 21, 173
Fsp4HI GCNGC 4 cut(s) 15, 18, 21, 173
FspBI CTAG 1 cut(s) 393
GluI GCNGC 4 cut(s) 15, 18, 21, 173
GsaI CCCAGC 1 cut(s) 420
HaeIII GGCC 2 cut(s) 88, 266
HapII CCGG 3 cut(s) 89, 263, 554
HgaI GACGC 2 cut(s) 424, 436
Hin1I GRCGYC 1 cut(s) 435
Hin1II CATG 5 cut(s) 262, 271, 331, 353, 568
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HindIII AAGCTT 1 cut(s) 520
HinfI GANTC 3 cut(s) 230, 373, 456
HpaII CCGG 3 cut(s) 89, 263, 554
HphI GGTGA 3 cut(s) 17, 225, 350
Hpy166II GTNNAC 2 cut(s) 292, 439
Hpy188I TCNGA 4 cut(s) 407, 429, 469, 497
Hpy188III TCNNGA 2 cut(s) 115, 377
Hpy8I GTNNAC 2 cut(s) 292, 439
HpyCH4IV ACGT 1 cut(s) 387
HpyCH4V TGCA 5 cut(s) 172, 239, 271, 299, 321
HpyF10VI GCNNNNNNNGC 3 cut(s) 20, 181, 268
HpyF3I CTNAG 1 cut(s) 443
HpySE526I ACGT 1 cut(s) 387
Hsp92I GRCGYC 1 cut(s) 435
Hsp92II CATG 5 cut(s) 262, 271, 331, 353, 568
KflI GGGWCCC 1 cut(s) 550
KroI GCCGGC 1 cut(s) 262
KroNI GCCGGC 1 cut(s) 264
Kzo9I GATC 2 cut(s) 111, 424
LmnI GCTCC 2 cut(s) 181, 223
Lsp1109I GCAGC 3 cut(s) 4, 7, 184
LweI GCATC 3 cut(s) 159, 205, 308
MaeI CTAG 1 cut(s) 393
MaeII ACGT 1 cut(s) 387
MaeIII GTNAC 2 cut(s) 231, 388
MalI GATC 2 cut(s) 113, 426
MboI GATC 2 cut(s) 111, 424
MboII GAAGA 3 cut(s) 172, 254, 532
MhlI GDGCHC 1 cut(s) 45
MluCI AATT 1 cut(s) 252
MlyI GAGTC 1 cut(s) 239
MnlI CCTC 7 cut(s) 231, 354, 357, 366, 401, 452, 546
MroNI GCCGGC 1 cut(s) 262
MseI TTAA 3 cut(s) 152, 188, 518
MspCI CTTAAG 1 cut(s) 517
MspI CCGG 3 cut(s) 89, 263, 554
MspR9I CCNGG 2 cut(s) 66, 554
MvaI CCWGG 1 cut(s) 66
MwoI GCNNNNNNNGC 3 cut(s) 20, 181, 268
NaeI GCCGGC 1 cut(s) 264
NciI CCSGG 1 cut(s) 554
NcoI CCATGG 1 cut(s) 327
NdeII GATC 2 cut(s) 111, 424
NgoMIV GCCGGC 1 cut(s) 262
NlaIII CATG 5 cut(s) 262, 271, 331, 353, 568
NlaIV GGNNCC 3 cut(s) 183, 551, 552
NmuCI GTSAC 1 cut(s) 231
NspI RCATGY 1 cut(s) 262
PaqCI CACCTGC 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 164
PdiI GCCGGC 1 cut(s) 264
PfeI GAWTC 2 cut(s) 373, 456
PflFI GACNNNGTC 1 cut(s) 560
PfoI TCCNGGA 1 cut(s) 552
PkrI GCNGC 4 cut(s) 16, 19, 22, 174
PleI GAGTC 1 cut(s) 238
PpsI GAGTC 1 cut(s) 238
PpuMI RGGWCCY 1 cut(s) 550
Psp5II RGGWCCY 1 cut(s) 550
Psp6I CCWGG 1 cut(s) 64
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 1 cut(s) 64
PspN4I GGNNCC 3 cut(s) 183, 551, 552
PspPI GGNCC 2 cut(s) 86, 550
PspPPI RGGWCCY 1 cut(s) 550
PstI CTGCAG 1 cut(s) 241
PsyI GACNNNGTC 1 cut(s) 560
RsaI GTAC 1 cut(s) 531
RsaNI GTAC 1 cut(s) 530
SaqAI TTAA 3 cut(s) 152, 188, 518
SatI GCNGC 4 cut(s) 15, 18, 21, 173
Sau3AI GATC 2 cut(s) 111, 424
Sau96I GGNCC 2 cut(s) 86, 550
SchI GAGTC 1 cut(s) 239
ScrFI CCNGG 2 cut(s) 66, 554
SduI GDGCHC 1 cut(s) 45
SetI ASST 8 cut(s) 238, 297, 384, 390, 444, 491, 524, 538
SfaNI GCATC 3 cut(s) 159, 205, 308
SfcI CTRYAG 2 cut(s) 36, 237
SinI GGWCC 1 cut(s) 550
SmlI CTYRAG 2 cut(s) 377, 517
SmoI CTYRAG 2 cut(s) 377, 517
Sse9I AATT 1 cut(s) 252
SsiI CCGC 2 cut(s) 72, 146
SspMI CTAG 1 cut(s) 393
StyD4I CCNGG 2 cut(s) 64, 552
StyI CCWWGG 1 cut(s) 327
TaiI ACGT 1 cut(s) 390
TaqI TCGA 2 cut(s) 158, 507
TasI AATT 1 cut(s) 252
TfiI GAWTC 2 cut(s) 373, 456
Tru1I TTAA 3 cut(s) 152, 188, 518
Tru9I TTAA 3 cut(s) 152, 188, 518
TscAI CASTG 1 cut(s) 50
TseFI GTSAC 1 cut(s) 231
TseI GCWGC 4 cut(s) 14, 17, 20, 172
Tsp45I GTSAC 1 cut(s) 231
TspDTI ATGAA 1 cut(s) 514
TspRI CASTG 1 cut(s) 50
Tth111I GACNNNGTC 1 cut(s) 560
Vha464I CTTAAG 1 cut(s) 517
VpaK11BI GGWCC 1 cut(s) 550
XapI RAATTY 1 cut(s) 252
XceI RCATGY 1 cut(s) 262
XmiI GTMKAC 1 cut(s) 291
XspI CTAG 1 cut(s) 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.