Rroxscaffold_2G00083660

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003399
Physical Location & Seq
Forward (+)
6324254 .. 6325433
1180 bp
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UTR
Exon/CDS
Intron
Rroxscaffold_2G00083660.1

Sequence Viewer

Length: 651 bp
ATGGTTGAGGCAATTGCTGTTTGCAGAATTATTGATAGAGGTATGCCTTCTCGCCCAGAAAAGCTGAAGGTGAAGCTGAAAAAGAAGCAGCGAGACAGGCATTGCCATTATGCAAAGGCGCTATTCAACTTGCCAAAAAAGGATGACCAGATGAAACCTTTAACCTTGCACCACAGAATCACTACTGTTGCCCTACTCTGTGAGTCCGCCAAGAAAGAGTGGAGGATTCAAATGCCAACCATGCAATTCTTGTTCATGTCTGTCTGCCTTGTATTATACATCAATTTAAGATACAAGTCCAAAGGGAAAGACTACCCTCCTCATGTTCCTCATCACACCCCCATGGTCCTTGAGCTTGAAGCCACAAGGTGGGAACTTGAGGGCTTCCCCTATGAGTACCGGCCGCGTGACTTTAGCATATCTGCTAAAGGAACCATATATCCTGATAGAAAGCAAGGAACACAAAGCAGGCTTAGAACCCAAATTGACGTAATCTTAAGCTTCATTGTTTCACCTCTGCTTGGTTGGGTTCCACAAAGTGTTATGCATAGCCTCATAAAATCGCTGGTGAAGACTGTGATGGCGGATTCGACATCAAATGCCAGATTGCTTGAAGACTATGATGAATTCAAAACGAAGAATGTGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

216

Amino Acids

25.2

Weight (kDa)

9.69

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 70 - 210 3.3e-19 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 369
AccII CGCG 1 cut(s) 406
AciI CCGC 3 cut(s) 207, 404, 584
AcoI YGGCCR 1 cut(s) 401
AcsI RAATTY 1 cut(s) 626
AcuI CTGAAG 1 cut(s) 86
AdeI CACNNNGTG 2 cut(s) 369, 539
AfaI GTAC 1 cut(s) 398
AfiI CCNNNNNNNGG 2 cut(s) 369, 521
AflII CTTAAG 1 cut(s) 496
AgsI TTSAA 5 cut(s) 127, 230, 359, 614, 631
AloI GAACNNNNNNTCC 2 cut(s) 424, 456
AluBI AGCT 4 cut(s) 64, 76, 355, 501
AluI AGCT 4 cut(s) 64, 76, 355, 501
Alw26I GTCTC 1 cut(s) 87
AoxI GGCC 1 cut(s) 401
ApeKI GCWGC 1 cut(s) 88
ApoI RAATTY 1 cut(s) 626
AspLEI GCGC 1 cut(s) 121
AspS9I GGNCC 1 cut(s) 346
AsuHPI GGTGA 3 cut(s) 82, 504, 580
AvaII GGWCC 1 cut(s) 346
BbsI GAAGAC 2 cut(s) 578, 621
BbvI GCAGC 1 cut(s) 100
BccI CCATC 1 cut(s) 574
BcoDI GTCTC 1 cut(s) 87
BfoI RGCGCY 1 cut(s) 122
BfrI CTTAAG 1 cut(s) 496
BisI GCNGC 2 cut(s) 89, 404
BlsI GCNGC 2 cut(s) 90, 405
Bme18I GGWCC 1 cut(s) 346
BmgT120I GGNCC 1 cut(s) 346
BmiI GGNNCC 2 cut(s) 433, 531
BpiI GAAGAC 2 cut(s) 578, 621
BpuEI CTTGAG 2 cut(s) 371, 398
BsaJI CCNNGG 1 cut(s) 342
Bsc4I CCNNNNNNNGG 2 cut(s) 369, 521
Bse118I RCCGGY 1 cut(s) 399
Bse3DI GCAATG 1 cut(s) 100
BseDI CCNNGG 1 cut(s) 342
BseGI GGATG 1 cut(s) 148
BseLI CCNNNNNNNGG 2 cut(s) 369, 521
BseMI GCAATG 1 cut(s) 100
BseRI GAGGAG 1 cut(s) 309
BseX3I CGGCCG 1 cut(s) 401
BseXI GCAGC 1 cut(s) 100
Bsh1236I CGCG 1 cut(s) 406
Bsh1285I CGRYCG 1 cut(s) 404
BshFI GGCC 1 cut(s) 403
BsiEI CGRYCG 1 cut(s) 404
BsiSI CCGG 1 cut(s) 400
BslI CCNNNNNNNGG 2 cut(s) 369, 521
BsmAI GTCTC 1 cut(s) 87
BsnI GGCC 1 cut(s) 403
Bsp19I CCATGG 1 cut(s) 342
BspACI CCGC 3 cut(s) 207, 404, 584
BspANI GGCC 1 cut(s) 403
BspFNI CGCG 1 cut(s) 406
BspLI GGNNCC 2 cut(s) 433, 531
BspTI CTTAAG 1 cut(s) 496
BsrDI GCAATG 1 cut(s) 100
BsrFI RCCGGY 1 cut(s) 399
BssAI RCCGGY 1 cut(s) 399
BssECI CCNNGG 1 cut(s) 342
BssT1I CCWWGG 1 cut(s) 342
Bst4CI ACNGT 2 cut(s) 187, 577
BstAFI CTTAAG 1 cut(s) 496
BstC8I GCNNGC 1 cut(s) 470
BstDEI CTNAG 1 cut(s) 473
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 148
BstFNI CGCG 1 cut(s) 406
BstH2I RGCGCY 1 cut(s) 122
BstHHI GCGC 1 cut(s) 121
BstMAI GTCTC 1 cut(s) 87
BstMCI CGRYCG 1 cut(s) 404
BstMWI GCNNNNNNNGC 2 cut(s) 97, 241
BstUI CGCG 1 cut(s) 406
BstV1I GCAGC 1 cut(s) 100
BstV2I GAAGAC 2 cut(s) 578, 621
BstZI CGGCCG 1 cut(s) 401
BsuRI GGCC 1 cut(s) 403
BtgI CCRYGG 1 cut(s) 342
BtsCI GGATG 1 cut(s) 148
Cac8I GCNNGC 1 cut(s) 470
CfoI GCGC 1 cut(s) 121
Cfr10I RCCGGY 1 cut(s) 399
Cfr13I GGNCC 1 cut(s) 346
Csp6I GTAC 1 cut(s) 397
CviAII CATG 4 cut(s) 241, 256, 323, 343
CviJI RGCY 9 cut(s) 64, 76, 355, 362, 384, 403, 472, 501, 552
CviKI_1 RGCY 9 cut(s) 64, 76, 355, 362, 384, 403, 472, 501, 552
CviQI GTAC 1 cut(s) 397
DdeI CTNAG 1 cut(s) 473
DraIII CACNNNGTG 2 cut(s) 369, 539
EaeI YGGCCR 1 cut(s) 401
EagI CGGCCG 1 cut(s) 401
EciI GGCGGA 2 cut(s) 196, 599
EclXI CGGCCG 1 cut(s) 401
Eco130I CCWWGG 1 cut(s) 342
Eco47I GGWCC 1 cut(s) 346
Eco52I CGGCCG 1 cut(s) 401
Eco57I CTGAAG 1 cut(s) 86
EcoRI GAATTC 1 cut(s) 626
EcoT14I CCWWGG 1 cut(s) 342
EcoT22I ATGCAT 1 cut(s) 549
ErhI CCWWGG 1 cut(s) 342
FaeI CATG 4 cut(s) 244, 259, 326, 346
FatI CATG 4 cut(s) 240, 255, 322, 342
Fnu4HI GCNGC 2 cut(s) 89, 404
FokI GGATG 1 cut(s) 155
Fsp4HI GCNGC 2 cut(s) 89, 404
GlaI GCGC 1 cut(s) 120
GluI GCNGC 2 cut(s) 89, 404
HaeII RGCGCY 1 cut(s) 122
HaeIII GGCC 1 cut(s) 403
HapII CCGG 1 cut(s) 400
HhaI GCGC 1 cut(s) 121
Hin1II CATG 4 cut(s) 244, 259, 326, 346
Hin6I GCGC 1 cut(s) 119
HinP1I GCGC 1 cut(s) 119
HindIII AAGCTT 1 cut(s) 499
HinfI GANTC 4 cut(s) 177, 203, 226, 587
HpaII CCGG 1 cut(s) 400
HphI GGTGA 3 cut(s) 82, 504, 580
Hpy188III TCNNGA 1 cut(s) 443
HpyAV CCTTC 2 cut(s) 57, 61
HpyCH4III ACNGT 2 cut(s) 187, 577
HpyCH4IV ACGT 1 cut(s) 489
HpyCH4V TGCA 5 cut(s) 24, 113, 169, 244, 547
HpyF10VI GCNNNNNNNGC 2 cut(s) 97, 241
HpyF3I CTNAG 1 cut(s) 473
HpySE526I ACGT 1 cut(s) 489
Hsp92II CATG 4 cut(s) 244, 259, 326, 346
HspAI GCGC 1 cut(s) 119
LpnPI CCDG 8 cut(s) 69, 82, 161, 413, 454, 456, 551, 616
Lsp1109I GCAGC 1 cut(s) 100
MaeII ACGT 1 cut(s) 489
MaeIII GTNAC 1 cut(s) 407
MboII GAAGA 3 cut(s) 583, 626, 649
MfeI CAATTG 1 cut(s) 12
MluCI AATT 6 cut(s) 12, 27, 245, 283, 483, 626
MlyI GAGTC 1 cut(s) 212
MnlI CCTC 8 cut(s) 32, 216, 327, 330, 339, 373, 525, 563
Mph1103I ATGCAT 1 cut(s) 549
MseI TTAA 3 cut(s) 161, 287, 497
MslI CAYNNNNRTG 1 cut(s) 341
MspCI CTTAAG 1 cut(s) 496
MspI CCGG 1 cut(s) 400
MunI CAATTG 1 cut(s) 12
MvnI CGCG 1 cut(s) 406
MwoI GCNNNNNNNGC 2 cut(s) 97, 241
NcoI CCATGG 1 cut(s) 342
NlaIII CATG 4 cut(s) 244, 259, 326, 346
NlaIV GGNNCC 2 cut(s) 433, 531
NmuCI GTSAC 1 cut(s) 407
NsiI ATGCAT 1 cut(s) 549
PfeI GAWTC 3 cut(s) 177, 226, 587
PflMI CCANNNNNTGG 1 cut(s) 369
PkrI GCNGC 2 cut(s) 90, 405
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
PspN4I GGNNCC 2 cut(s) 433, 531
PspPI GGNCC 1 cut(s) 346
RsaI GTAC 1 cut(s) 398
RsaNI GTAC 1 cut(s) 397
RseI CAYNNNNRTG 1 cut(s) 341
SaqAI TTAA 3 cut(s) 161, 287, 497
SatI GCNGC 2 cut(s) 89, 404
Sau96I GGNCC 1 cut(s) 346
SchI GAGTC 1 cut(s) 212
SinI GGWCC 1 cut(s) 346
SmiMI CAYNNNNRTG 1 cut(s) 341
SmlI CTYRAG 3 cut(s) 350, 377, 496
SmoI CTYRAG 3 cut(s) 350, 377, 496
Sse9I AATT 6 cut(s) 12, 27, 245, 283, 483, 626
SsiI CCGC 3 cut(s) 207, 404, 584
StyI CCWWGG 1 cut(s) 342
TaaI ACNGT 2 cut(s) 187, 577
TaiI ACGT 1 cut(s) 492
TaqI TCGA 1 cut(s) 590
TasI AATT 6 cut(s) 12, 27, 245, 283, 483, 626
TauI GCSGC 1 cut(s) 406
TfiI GAWTC 3 cut(s) 177, 226, 587
Tru1I TTAA 3 cut(s) 161, 287, 497
Tru9I TTAA 3 cut(s) 161, 287, 497
TseFI GTSAC 1 cut(s) 407
TseI GCWGC 1 cut(s) 88
Tsp45I GTSAC 1 cut(s) 407
TspDTI ATGAA 4 cut(s) 167, 244, 493, 639
Van91I CCANNNNNTGG 1 cut(s) 369
Vha464I CTTAAG 1 cut(s) 496
VpaK11BI GGWCC 1 cut(s) 346
XapI RAATTY 1 cut(s) 626
Zsp2I ATGCAT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.