MD09G1055000.v1.1

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0003399
Physical Location & Seq
Forward (+)
3656571 .. 3658237
1667 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1055000.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGGTGAGTTTGCTGCTGCTGCTGCTGGTCTTTTTTCTGTAGGGCACTGCCAACCCTTGTTCTGTCCTGGTAGCGGAATGTGTTTCGGGCCGGAAAATGTAGTGTGTAAGCCGATCAGGAAGCAGGAGAGGATTTGCCGTGCTAAAGCGGTGTTAAAATCGAAGAAAACGATGCAGCAGAGGGAGCCTTTAAACTTTTACTTTAGATATACATTTGATGCTCCTCTCAATGAGTCCCCTGCAGCTTCATTTGATCAATACTTGGAAGATGAACACAGGGTTTTTAAAGCGATTTTTCCGGACACAGCGAAAACCCAAACAAAAGAGGAAGAGTGGAGAATTCACATGCCGGCCATGCAAGTCTTGTTCCTGTCTGTCTACCTTGCATTATATGTAAAGTTGAGATGCAAATCCATGGGGAAAGACTACCCTCCTCATGTTCCTCACCACATCTCCAAGATTCTTGAGCTTGACGTTACTAGATGGGAAATCCGAGGCATTGCTGGGTATGATCCAAGTGACATCAACCTTAGCGTCAAAGGAACCATATTTTCAGATAGACAAGGAACACAAAGCTGGCTCAGAAACATATTCGACATAAACTTAAGCTTCATCGTACCACCTCTCCTCGCTTGGGTCCCAGACAATACCATGCAAAGCATTACAGAATCGCTCGTGAAGACGGTGATGGCCAATCTGAAGTCGAATGCAATGTTGCTAGCAGTAGCAGATTATGAGAAGTTCAAAAAAGAGAAGCTCAAAACTGCAGTAAATTCTAAGAAATTAAGGGGCAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

266

Amino Acids

30.11

Weight (kDa)

9.21

Isoelectric Point (pI)

35.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 84 - 248 8.6e-25 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 378
AccIII TCCGGA 1 cut(s) 298
AciI CCGC 2 cut(s) 75, 149
AclWI GGATC 1 cut(s) 506
AcoI YGGCCR 2 cut(s) 351, 690
AcsI RAATTY 2 cut(s) 339, 772
AcuI CTGAAG 1 cut(s) 719
AfaI GTAC 1 cut(s) 618
AfiI CCNNNNNNNGG 2 cut(s) 74, 634
AflII CTTAAG 1 cut(s) 604
AgsI TTSAA 1 cut(s) 745
AjnI CCWGG 1 cut(s) 67
AluBI AGCT 6 cut(s) 245, 469, 576, 609, 757, 795
AluI AGCT 6 cut(s) 245, 469, 576, 609, 757, 795
AlwI GGATC 1 cut(s) 506
Aor13HI TCCGGA 1 cut(s) 298
AoxI GGCC 3 cut(s) 89, 351, 690
ApeKI GCWGC 7 cut(s) 14, 17, 20, 23, 175, 242, 792
ApoI RAATTY 2 cut(s) 339, 772
AspS9I GGNCC 2 cut(s) 89, 637
AsuHPI GGTGA 3 cut(s) 17, 437, 697
AsuNHI GCTAGC 1 cut(s) 718
AvaII GGWCC 1 cut(s) 637
BaeGI GKGCMC 1 cut(s) 48
BalI TGGCCA 1 cut(s) 692
BauI CACGAG 1 cut(s) 674
BbsI GAAGAC 1 cut(s) 686
BbvI GCAGC 5 cut(s) 4, 7, 10, 187, 254
BccI CCATC 2 cut(s) 477, 682
BceAI ACGGC 1 cut(s) 123
BciT130I CCWGG 1 cut(s) 69
BclI TGATCA 1 cut(s) 253
BfaI CTAG 2 cut(s) 480, 719
BfmI CTRYAG 3 cut(s) 39, 240, 765
BfrI CTTAAG 1 cut(s) 604
BisI GCNGC 7 cut(s) 15, 18, 21, 24, 176, 243, 793
BlsI GCNGC 7 cut(s) 16, 19, 22, 25, 177, 244, 794
Bme1390I CCNGG 1 cut(s) 69
Bme18I GGWCC 1 cut(s) 637
BmgT120I GGNCC 2 cut(s) 89, 637
BmiI GGNNCC 4 cut(s) 186, 544, 638, 639
BmrFI CCNGG 1 cut(s) 69
BmsI GCATC 3 cut(s) 162, 208, 395
BmtI GCTAGC 1 cut(s) 722
BpiI GAAGAC 1 cut(s) 686
Bpu10I CCTNAGC 1 cut(s) 530
BpuEI CTTGAG 1 cut(s) 485
BsaBI GATNNNNATC 1 cut(s) 409
BsaJI CCNNGG 2 cut(s) 414, 493
BsaWI WCCGGW 1 cut(s) 298
BsaXI ACNNNNNCTCC 4 cut(s) 437, 467, 609, 639
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 634
Bse118I RCCGGY 1 cut(s) 349
Bse3DI GCAATG 2 cut(s) 498, 717
Bse8I GATNNNNATC 1 cut(s) 409
BseAI TCCGGA 1 cut(s) 298
BseBI CCWGG 1 cut(s) 69
BseDI CCNNGG 2 cut(s) 414, 493
BseJI GATNNNNATC 1 cut(s) 409
BseLI CCNNNNNNNGG 2 cut(s) 74, 634
BseMI GCAATG 2 cut(s) 498, 717
BseMII CTCAG 1 cut(s) 595
BseRI GAGGAG 3 cut(s) 213, 423, 617
BseSI GKGCMC 1 cut(s) 48
BseXI GCAGC 5 cut(s) 4, 7, 10, 187, 254
BseYI CCCAGC 1 cut(s) 503
BshFI GGCC 3 cut(s) 91, 353, 692
BsiSI CCGG 3 cut(s) 92, 299, 350
BslFI GGGAC 2 cut(s) 220, 623
BslI CCNNNNNNNGG 2 cut(s) 74, 634
BsmFI GGGAC 2 cut(s) 220, 623
BsmI GAATGC 1 cut(s) 712
BsnI GGCC 3 cut(s) 91, 353, 692
Bsp1286I GDGCHC 1 cut(s) 48
Bsp13I TCCGGA 1 cut(s) 298
Bsp143I GATC 3 cut(s) 114, 253, 511
Bsp19I CCATGG 1 cut(s) 414
BspACI CCGC 2 cut(s) 75, 149
BspANI GGCC 3 cut(s) 91, 353, 692
BspCNI CTCAG 1 cut(s) 594
BspEI TCCGGA 1 cut(s) 298
BspLI GGNNCC 4 cut(s) 186, 544, 638, 639
BspMAI CTGCAG 2 cut(s) 244, 769
BspOI GCTAGC 1 cut(s) 722
BspPI GGATC 1 cut(s) 506
BspTI CTTAAG 1 cut(s) 604
BsrDI GCAATG 2 cut(s) 498, 717
BsrFI RCCGGY 1 cut(s) 349
BssAI RCCGGY 1 cut(s) 349
BssECI CCNNGG 2 cut(s) 414, 493
BssMI GATC 3 cut(s) 114, 253, 511
BssSI CACGAG 1 cut(s) 674
BssT1I CCWWGG 1 cut(s) 414
Bst2BI CACGAG 1 cut(s) 674
Bst2UI CCWGG 1 cut(s) 69
Bst4CI ACNGT 1 cut(s) 685
Bst6I CTCTTC 1 cut(s) 324
BstAFI CTTAAG 1 cut(s) 604
BstC8I GCNNGC 3 cut(s) 351, 578, 720
BstDEI CTNAG 3 cut(s) 530, 581, 777
BstDSI CCRYGG 1 cut(s) 414
BstKTI GATC 3 cut(s) 117, 256, 514
BstMBI GATC 3 cut(s) 114, 253, 511
BstMWI GCNNNNNNNGC 4 cut(s) 20, 23, 184, 355
BstNI CCWGG 1 cut(s) 69
BstNSI RCATGY 1 cut(s) 349
BstSCI CCNGG 1 cut(s) 67
BstSFI CTRYAG 3 cut(s) 39, 240, 765
BstSLI GKGCMC 1 cut(s) 48
BstV1I GCAGC 5 cut(s) 4, 7, 10, 187, 254
BstV2I GAAGAC 1 cut(s) 686
BsuRI GGCC 3 cut(s) 91, 353, 692
BtgI CCRYGG 1 cut(s) 414
BtsI GCAGTG 1 cut(s) 46
BtsIMutI CAGTG 1 cut(s) 46
Cac8I GCNNGC 3 cut(s) 351, 578, 720
Cfr10I RCCGGY 1 cut(s) 349
Cfr13I GGNCC 2 cut(s) 89, 637
CseI GACGC 1 cut(s) 523
Csp6I GTAC 1 cut(s) 617
CviAII CATG 5 cut(s) 346, 355, 415, 437, 652
CviQI GTAC 1 cut(s) 617
DdeI CTNAG 3 cut(s) 530, 581, 777
DpnI GATC 3 cut(s) 116, 255, 513
DpnII GATC 3 cut(s) 114, 253, 511
DraI TTTAAA 2 cut(s) 192, 286
EaeI YGGCCR 2 cut(s) 351, 690
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 414
Eco47I GGWCC 1 cut(s) 637
Eco57I CTGAAG 1 cut(s) 719
EcoO109I RGGNCCY 1 cut(s) 637
EcoRI GAATTC 1 cut(s) 339
EcoRII CCWGG 1 cut(s) 67
EcoT14I CCWWGG 1 cut(s) 414
ErhI CCWWGG 1 cut(s) 414
FaeI CATG 5 cut(s) 349, 358, 418, 440, 655
FaqI GGGAC 2 cut(s) 220, 623
FatI CATG 5 cut(s) 345, 354, 414, 436, 651
FbaI TGATCA 1 cut(s) 253
FblI GTMKAC 1 cut(s) 378
Fnu4HI GCNGC 7 cut(s) 15, 18, 21, 24, 176, 243, 793
Fsp4HI GCNGC 7 cut(s) 15, 18, 21, 24, 176, 243, 793
FspBI CTAG 2 cut(s) 480, 719
GluI GCNGC 7 cut(s) 15, 18, 21, 24, 176, 243, 793
GsaI CCCAGC 1 cut(s) 507
HaeIII GGCC 3 cut(s) 91, 353, 692
HapII CCGG 3 cut(s) 92, 299, 350
HgaI GACGC 1 cut(s) 523
Hin1II CATG 5 cut(s) 349, 358, 418, 440, 655
HindIII AAGCTT 1 cut(s) 607
HinfI GANTC 3 cut(s) 233, 460, 668
HpaII CCGG 3 cut(s) 92, 299, 350
HphI GGTGA 3 cut(s) 17, 437, 697
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 4 cut(s) 494, 556, 584, 699
Hpy188III TCNNGA 4 cut(s) 118, 299, 464, 676
Hpy8I GTNNAC 1 cut(s) 379
HpyCH4III ACNGT 1 cut(s) 685
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 8 cut(s) 175, 242, 358, 386, 408, 655, 710, 767
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 23, 184, 355
HpyF3I CTNAG 3 cut(s) 530, 581, 777
HpySE526I ACGT 1 cut(s) 474
Hsp92II CATG 5 cut(s) 349, 358, 418, 440, 655
KflI GGGWCCC 1 cut(s) 637
Kpn2I TCCGGA 1 cut(s) 298
KroI GCCGGC 1 cut(s) 349
KroNI GCCGGC 1 cut(s) 351
Ksp22I TGATCA 1 cut(s) 253
Kzo9I GATC 3 cut(s) 114, 253, 511
LmnI GCTCC 2 cut(s) 184, 226
Lsp1109I GCAGC 5 cut(s) 4, 7, 10, 187, 254
LweI GCATC 3 cut(s) 162, 208, 395
MaeI CTAG 2 cut(s) 480, 719
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 2 cut(s) 475, 518
MalI GATC 3 cut(s) 116, 255, 513
MboI GATC 3 cut(s) 114, 253, 511
MboII GAAGA 4 cut(s) 175, 278, 341, 691
MhlI GDGCHC 1 cut(s) 48
MlsI TGGCCA 1 cut(s) 692
MluCI AATT 3 cut(s) 339, 772, 782
MluNI TGGCCA 1 cut(s) 692
MlyI GAGTC 1 cut(s) 242
Mox20I TGGCCA 1 cut(s) 692
MroI TCCGGA 1 cut(s) 298
MroNI GCCGGC 1 cut(s) 349
MscI TGGCCA 1 cut(s) 692
MseI TTAA 5 cut(s) 155, 191, 285, 605, 785
Msp20I TGGCCA 1 cut(s) 692
MspCI CTTAAG 1 cut(s) 604
MspI CCGG 3 cut(s) 92, 299, 350
MspR9I CCNGG 1 cut(s) 69
Mva1269I GAATGC 1 cut(s) 712
MvaI CCWGG 1 cut(s) 69
MwoI GCNNNNNNNGC 4 cut(s) 20, 23, 184, 355
NaeI GCCGGC 1 cut(s) 351
NcoI CCATGG 1 cut(s) 414
NdeII GATC 3 cut(s) 114, 253, 511
NgoMIV GCCGGC 1 cut(s) 349
NheI GCTAGC 1 cut(s) 718
NlaIII CATG 5 cut(s) 349, 358, 418, 440, 655
NlaIV GGNNCC 4 cut(s) 186, 544, 638, 639
NmuCI GTSAC 1 cut(s) 518
NspI RCATGY 1 cut(s) 349
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PctI GAATGC 1 cut(s) 712
PdiI GCCGGC 1 cut(s) 351
PfeI GAWTC 2 cut(s) 460, 668
PkrI GCNGC 7 cut(s) 16, 19, 22, 25, 177, 244, 794
PleI GAGTC 1 cut(s) 241
PpsI GAGTC 1 cut(s) 241
PpuMI RGGWCCY 1 cut(s) 637
Psp5II RGGWCCY 1 cut(s) 637
Psp6I CCWGG 1 cut(s) 67
PspFI CCCAGC 1 cut(s) 503
PspGI CCWGG 1 cut(s) 67
PspN4I GGNNCC 4 cut(s) 186, 544, 638, 639
PspPI GGNCC 2 cut(s) 89, 637
PspPPI RGGWCCY 1 cut(s) 637
PstI CTGCAG 2 cut(s) 244, 769
RsaI GTAC 1 cut(s) 618
RsaNI GTAC 1 cut(s) 617
SaqAI TTAA 5 cut(s) 155, 191, 285, 605, 785
SatI GCNGC 7 cut(s) 15, 18, 21, 24, 176, 243, 793
Sau3AI GATC 3 cut(s) 114, 253, 511
Sau96I GGNCC 2 cut(s) 89, 637
SchI GAGTC 1 cut(s) 242
ScrFI CCNGG 1 cut(s) 69
SduI GDGCHC 1 cut(s) 48
SfaNI GCATC 3 cut(s) 162, 208, 395
SfcI CTRYAG 3 cut(s) 39, 240, 765
SinI GGWCC 1 cut(s) 637
SmlI CTYRAG 2 cut(s) 464, 604
SmoI CTYRAG 2 cut(s) 464, 604
Sse9I AATT 3 cut(s) 339, 772, 782
SsiI CCGC 2 cut(s) 75, 149
SspMI CTAG 2 cut(s) 480, 719
StyD4I CCNGG 1 cut(s) 67
StyI CCWWGG 1 cut(s) 414
TaaI ACNGT 1 cut(s) 685
TaiI ACGT 1 cut(s) 477
TaqI TCGA 3 cut(s) 161, 594, 704
TasI AATT 3 cut(s) 339, 772, 782
TfiI GAWTC 2 cut(s) 460, 668
Tru1I TTAA 5 cut(s) 155, 191, 285, 605, 785
Tru9I TTAA 5 cut(s) 155, 191, 285, 605, 785
TscAI CASTG 1 cut(s) 53
TseFI GTSAC 1 cut(s) 518
TseI GCWGC 7 cut(s) 14, 17, 20, 23, 175, 242, 792
Tsp45I GTSAC 1 cut(s) 518
TspDTI ATGAA 3 cut(s) 237, 285, 601
TspRI CASTG 1 cut(s) 53
Vha464I CTTAAG 1 cut(s) 604
VpaK11BI GGWCC 1 cut(s) 637
XapI RAATTY 2 cut(s) 339, 772
XceI RCATGY 1 cut(s) 349
XmiI GTMKAC 1 cut(s) 378
XspI CTAG 2 cut(s) 480, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.