Rh2AG596200

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003399
Physical Location & Seq
Reverse (-)
82638429 .. 82660960
22532 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG596200.1

Sequence Viewer

Length: 729 bp
ATGGTTGGGGTAGTTGCTTTTTGCCGACCCTTGTTTATTCTTGGTACTAGTCTGCCTTTTCCAGAAAAATTGAAGGTGAAGCTGAAGAAGGGCAGACAGCGCCACTATGCGAAAGCGGTATGGAACTCAGATCATAAGAAGCCTTTTACCGTGAACCTCAGTTCCACTATTGATGCACCTCTGTATGAGTCCCCTGAGGTTTCATTTGATCAATATTTGGAGGATGAACAAAGGGTTATTAAAGCACTTTTTCCAGACATGGAGGACCAGCAAATTAGCAAGGAAGAGTGGAGGATTCAAATCCCAACCATGCAAGTCTTGTTCCTGTCTATCTACTTTGCAATGTACATCAAGTTAAGATTCAAATCCATGGGGAAAGACTACCCTCCTCATGTTCCTCAGCACATTCCCAAAATCACTGAGCTTGAAATTACAAGATGGGAACTTGGGGGGATCCCTGAGGAGTGCCAGCCGCTTGACTTGAGCCTCTCCCTCAAAGGAACCATATATCCTGATAGAAAGCAAGGAACAAAAAGCAGGCTTAGAGGTCAGGTTGACATAAATATGAGCTTTATTGTATCACCTCTGCTTGGTTGGATTCCTCATACTGTCATGCAGAGCCTTGCAGAAGCGCTGGTTAAGAAAGCGATGGCAGATTCGACATCAAATTCCAGATTGGTAGAAGACTATGATGAATTTAAGACGGAGAAACTCAAGAATGTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

242

Amino Acids

27.9

Weight (kDa)

8.68

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 69 - 233 9e-23 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 116, 473
AclWI GGATC 2 cut(s) 448, 461
AcsI RAATTY 2 cut(s) 667, 695
AcuI CTGAAG 1 cut(s) 104
AfaI GTAC 2 cut(s) 46, 347
AfeI AGCGCT 1 cut(s) 633
AfiI CCNNNNNNNGG 1 cut(s) 590
AgsI TTSAA 4 cut(s) 73, 299, 364, 428
AhlI ACTAGT 1 cut(s) 47
AloI GAACNNNNNNTCC 4 cut(s) 146, 178, 493, 525
AluBI AGCT 3 cut(s) 82, 424, 570
AluI AGCT 3 cut(s) 82, 424, 570
AlwI GGATC 2 cut(s) 448, 461
Aor51HI AGCGCT 1 cut(s) 633
ApoI RAATTY 2 cut(s) 667, 695
AspLEI GCGC 2 cut(s) 102, 634
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 2 cut(s) 88, 573
AvaII GGWCC 1 cut(s) 265
AxyI CCTNAGG 2 cut(s) 195, 459
BamHI GGATCC 1 cut(s) 453
BbsI GAAGAC 1 cut(s) 690
BbvCI CCTCAGC 1 cut(s) 399
BccI CCATC 2 cut(s) 432, 643
BclI TGATCA 1 cut(s) 208
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 1 cut(s) 48
BfoI RGCGCY 2 cut(s) 103, 635
BisI GCNGC 1 cut(s) 473
BlsI GCNGC 1 cut(s) 474
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 2 cut(s) 455, 502
BmsI GCATC 1 cut(s) 163
BpiI GAAGAC 1 cut(s) 690
Bpu10I CCTNAGC 1 cut(s) 399
BpuEI CTTGAG 2 cut(s) 502, 698
BsaBI GATNNNNATC 2 cut(s) 299, 364
BsaJI CCNNGG 1 cut(s) 369
Bsc4I CCNNNNNNNGG 1 cut(s) 590
Bse21I CCTNAGG 2 cut(s) 195, 459
Bse3DI GCAATG 1 cut(s) 348
Bse8I GATNNNNATC 2 cut(s) 299, 364
BseDI CCNNGG 1 cut(s) 369
BseGI GGATG 1 cut(s) 229
BseJI GATNNNNATC 2 cut(s) 299, 364
BseLI CCNNNNNNNGG 1 cut(s) 590
BseMI GCAATG 1 cut(s) 348
BseMII CTCAG 6 cut(s) 141, 172, 186, 411, 413, 450
BseRI GAGGAG 2 cut(s) 378, 476
BslFI GGGAC 1 cut(s) 175
BslI CCNNNNNNNGG 1 cut(s) 590
BsmFI GGGAC 1 cut(s) 175
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 3 cut(s) 130, 208, 453
Bsp19I CCATGG 1 cut(s) 369
BspACI CCGC 2 cut(s) 116, 473
BspCNI CTCAG 6 cut(s) 140, 171, 187, 412, 412, 451
BspLI GGNNCC 2 cut(s) 455, 502
BspPI GGATC 2 cut(s) 448, 461
BsrDI GCAATG 1 cut(s) 348
BsrGI TGTACA 1 cut(s) 345
BssECI CCNNGG 1 cut(s) 369
BssMI GATC 3 cut(s) 130, 208, 453
BssT1I CCWWGG 1 cut(s) 369
Bst4CI ACNGT 2 cut(s) 151, 610
Bst6I CTCTTC 1 cut(s) 279
BstAUI TGTACA 1 cut(s) 345
BstC8I GCNNGC 2 cut(s) 470, 539
BstDEI CTNAG 7 cut(s) 127, 158, 195, 399, 420, 459, 542
BstDSI CCRYGG 1 cut(s) 369
BstF5I GGATG 1 cut(s) 229
BstH2I RGCGCY 2 cut(s) 103, 635
BstHHI GCGC 2 cut(s) 102, 634
BstKTI GATC 3 cut(s) 133, 211, 456
BstMBI GATC 3 cut(s) 130, 208, 453
BstMWI GCNNNNNNNGC 1 cut(s) 99
BstV2I GAAGAC 1 cut(s) 690
BstX2I RGATCY 1 cut(s) 453
BstYI RGATCY 1 cut(s) 453
Bsu36I CCTNAGG 2 cut(s) 195, 459
BtgI CCRYGG 1 cut(s) 369
BtgZI GCGATG 1 cut(s) 662
BtsCI GGATG 1 cut(s) 229
BtsIMutI CAGTG 1 cut(s) 417
Cac8I GCNNGC 2 cut(s) 470, 539
CfoI GCGC 2 cut(s) 102, 634
Cfr13I GGNCC 1 cut(s) 265
Csp6I GTAC 2 cut(s) 45, 346
CviAII CATG 5 cut(s) 259, 310, 370, 392, 613
CviJI RGCY 8 cut(s) 82, 142, 424, 472, 486, 541, 570, 621
CviKI_1 RGCY 8 cut(s) 82, 142, 424, 472, 486, 541, 570, 621
CviQI GTAC 2 cut(s) 45, 346
DdeI CTNAG 7 cut(s) 127, 158, 195, 399, 420, 459, 542
DpnI GATC 3 cut(s) 132, 210, 455
DpnII GATC 3 cut(s) 130, 208, 453
Eam1104I CTCTTC 1 cut(s) 279
EarI CTCTTC 1 cut(s) 279
Eco130I CCWWGG 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 265
Eco47III AGCGCT 1 cut(s) 633
Eco57I CTGAAG 1 cut(s) 104
Eco81I CCTNAGG 2 cut(s) 195, 459
EcoT14I CCWWGG 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 5 cut(s) 262, 313, 373, 395, 616
FaqI GGGAC 1 cut(s) 175
FatI CATG 5 cut(s) 258, 309, 369, 391, 612
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 1 cut(s) 473
FokI GGATG 1 cut(s) 236
Fsp4HI GCNGC 1 cut(s) 473
FspBI CTAG 1 cut(s) 48
GlaI GCGC 2 cut(s) 101, 633
GluI GCNGC 1 cut(s) 473
HaeII RGCGCY 2 cut(s) 103, 635
HhaI GCGC 2 cut(s) 102, 634
Hin1II CATG 5 cut(s) 262, 313, 373, 395, 616
Hin6I GCGC 2 cut(s) 100, 632
HinP1I GCGC 2 cut(s) 100, 632
HincII GTYRAC 1 cut(s) 556
HindII GTYRAC 1 cut(s) 556
HinfI GANTC 5 cut(s) 188, 295, 360, 598, 656
HphI GGTGA 2 cut(s) 88, 573
Hpy166II GTNNAC 2 cut(s) 154, 556
Hpy188I TCNGA 1 cut(s) 130
Hpy188III TCNNGA 5 cut(s) 62, 254, 512, 672, 715
Hpy8I GTNNAC 2 cut(s) 154, 556
HpyAV CCTTC 2 cut(s) 67, 82
HpyCH4III ACNGT 2 cut(s) 151, 610
HpyCH4V TGCA 5 cut(s) 176, 313, 341, 616, 626
HpyF10VI GCNNNNNNNGC 1 cut(s) 99
HpyF3I CTNAG 7 cut(s) 127, 158, 195, 399, 420, 459, 542
Hsp92II CATG 5 cut(s) 262, 313, 373, 395, 616
HspAI GCGC 2 cut(s) 100, 632
Ksp22I TGATCA 1 cut(s) 208
Kzo9I GATC 3 cut(s) 130, 208, 453
LweI GCATC 1 cut(s) 163
MaeI CTAG 1 cut(s) 48
MalI GATC 3 cut(s) 132, 210, 455
MboI GATC 3 cut(s) 130, 208, 453
MboII GAAGA 3 cut(s) 97, 296, 695
MflI RGATCY 1 cut(s) 453
MluCI AATT 5 cut(s) 68, 273, 429, 667, 695
MlyI GAGTC 1 cut(s) 197
MmeI TCCRAC 1 cut(s) 575
MseI TTAA 4 cut(s) 240, 356, 639, 699
MslI CAYNNNNRTG 1 cut(s) 563
MwoI GCNNNNNNNGC 1 cut(s) 99
NcoI CCATGG 1 cut(s) 369
NdeII GATC 3 cut(s) 130, 208, 453
NlaIII CATG 5 cut(s) 262, 313, 373, 395, 616
NlaIV GGNNCC 2 cut(s) 455, 502
PfeI GAWTC 4 cut(s) 295, 360, 598, 656
PkrI GCNGC 1 cut(s) 474
PleI GAGTC 1 cut(s) 196
PpsI GAGTC 1 cut(s) 196
PspN4I GGNNCC 2 cut(s) 455, 502
PspPI GGNCC 1 cut(s) 265
PsuI RGATCY 1 cut(s) 453
RsaI GTAC 2 cut(s) 46, 347
RsaNI GTAC 2 cut(s) 45, 346
RseI CAYNNNNRTG 1 cut(s) 563
SaqAI TTAA 4 cut(s) 240, 356, 639, 699
SatI GCNGC 1 cut(s) 473
Sau3AI GATC 3 cut(s) 130, 208, 453
Sau96I GGNCC 1 cut(s) 265
SchI GAGTC 1 cut(s) 197
SfaNI GCATC 1 cut(s) 163
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 1 cut(s) 563
SmlI CTYRAG 2 cut(s) 481, 713
SmoI CTYRAG 2 cut(s) 481, 713
SpeI ACTAGT 1 cut(s) 47
Sse9I AATT 5 cut(s) 68, 273, 429, 667, 695
SsiI CCGC 2 cut(s) 116, 473
SspI AATATT 1 cut(s) 215
SspMI CTAG 1 cut(s) 48
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 2 cut(s) 151, 610
TaqI TCGA 1 cut(s) 659
TasI AATT 5 cut(s) 68, 273, 429, 667, 695
TatI WGTACW 1 cut(s) 345
TauI GCSGC 1 cut(s) 475
TfiI GAWTC 4 cut(s) 295, 360, 598, 656
Tru1I TTAA 4 cut(s) 240, 356, 639, 699
Tru9I TTAA 4 cut(s) 240, 356, 639, 699
TscAI CASTG 1 cut(s) 424
TspDTI ATGAA 3 cut(s) 192, 240, 708
TspGWI ACGGA 1 cut(s) 719
TspRI CASTG 1 cut(s) 424
VpaK11BI GGWCC 1 cut(s) 265
XapI RAATTY 2 cut(s) 667, 695
XspI CTAG 1 cut(s) 48
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.