Rroxscaffold_2G00083650

Protein of unknown function (DUF1997)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003399
Physical Location & Seq
Forward (+)
6322381 .. 6323514
1134 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00083650.1

Sequence Viewer

Length: 582 bp
ATGGTTGAGGGAGTTGCTTTATGCCAACCCTTGTTTATTCTAGGTACTGGTATGCCTTTTCGCCCAGAAAAATTGAAGGTGAGGCTGAAGAAGGGCAGACGTCGCCATTATGCTATAGCGGTATTGAACTCGGATCATAAGAAGCCTTTAACCGTGAACCTCAGTTCCACTATTGATGCACCTCTTTATGAGTCCCCTGAGGTTTCATTTGATCAATATTTGGAGGATGAACAAAGGGTTATTAAAGCACTTTTTCCAGACCTGGAGGACCAGCAACTTAGCAAGACAAGATGGGAACTTGGGGGCATCCCTGAGGAGTACCAGCCGCGTGACTTGAGCCTCTCCCTCAAAGGAACCATATATCCTGATAGAAAGCAAGGAACACAAAGCAGGCTTAGAAGCCAGTTTGACCTAAACATGAGCTTTATTTTATCACCTCTGCTCGGTTGGGTTCCTCATAGTGTTATGCATAGCCTTACAGAAGCGCTGTTTAAGACAGCGATGGCTGATTCGATATCAAATGCCAGATTGCTAGAAGACTATGATGAATTCAAGATGGAGACTCTCAAGAACCTAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1997

Protein Analysis

193

Amino Acids

22.13

Weight (kDa)

6.44

Isoelectric Point (pI)

61.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1997 PF09366 93 - 184 8.9e-09 Protein of unknown function (DUF1997)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 103
AccII CGCG 1 cut(s) 328
AciI CCGC 2 cut(s) 119, 326
AclWI GGATC 1 cut(s) 141
AcsI RAATTY 1 cut(s) 548
AcuI CTGAAG 1 cut(s) 107
AcyI GRCGYC 1 cut(s) 100
AfaI GTAC 2 cut(s) 46, 320
AfeI AGCGCT 1 cut(s) 486
AfiI CCNNNNNNNGG 1 cut(s) 443
AgsI TTSAA 3 cut(s) 76, 127, 553
AjnI CCWGG 1 cut(s) 261
AloI GAACNNNNNNTCC 4 cut(s) 149, 181, 346, 378
AluBI AGCT 1 cut(s) 423
AluI AGCT 1 cut(s) 423
Alw26I GTCTC 1 cut(s) 554
AlwI GGATC 1 cut(s) 141
Aor51HI AGCGCT 1 cut(s) 486
ApoI RAATTY 1 cut(s) 548
AspLEI GCGC 1 cut(s) 487
AspS9I GGNCC 1 cut(s) 268
AsuHPI GGTGA 2 cut(s) 91, 426
AvaII GGWCC 1 cut(s) 268
AxyI CCTNAGG 2 cut(s) 198, 312
BbsI GAAGAC 1 cut(s) 543
BccI CCATC 3 cut(s) 285, 496, 550
BciT130I CCWGG 1 cut(s) 263
BclI TGATCA 1 cut(s) 211
BcoDI GTCTC 1 cut(s) 554
BfaI CTAG 3 cut(s) 41, 533, 575
BfmI CTRYAG 1 cut(s) 114
BfoI RGCGCY 1 cut(s) 488
BisI GCNGC 1 cut(s) 326
BlsI GCNGC 1 cut(s) 327
Bme1390I CCNGG 1 cut(s) 263
Bme18I GGWCC 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 268
BmiI GGNNCC 2 cut(s) 355, 453
BmrFI CCNGG 1 cut(s) 263
BmsI GCATC 2 cut(s) 166, 315
BpiI GAAGAC 1 cut(s) 543
BpmI CTGGAG 1 cut(s) 284
BpuEI CTTGAG 2 cut(s) 355, 551
BsaHI GRCGYC 1 cut(s) 100
Bsc4I CCNNNNNNNGG 1 cut(s) 443
Bse1I ACTGG 2 cut(s) 52, 403
Bse21I CCTNAGG 2 cut(s) 198, 312
BseBI CCWGG 1 cut(s) 263
BseGI GGATG 2 cut(s) 232, 306
BseLI CCNNNNNNNGG 1 cut(s) 443
BseMII CTCAG 3 cut(s) 175, 189, 303
BseNI ACTGG 2 cut(s) 52, 403
BseRI GAGGAG 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 328
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 1 cut(s) 443
BsmAI GTCTC 1 cut(s) 554
BsmFI GGGAC 1 cut(s) 178
Bsp143I GATC 2 cut(s) 133, 211
BspACI CCGC 2 cut(s) 119, 326
BspCNI CTCAG 3 cut(s) 174, 190, 304
BspFNI CGCG 1 cut(s) 328
BspLI GGNNCC 2 cut(s) 355, 453
BspPI GGATC 1 cut(s) 141
BsrI ACTGG 2 cut(s) 52, 403
BssMI GATC 2 cut(s) 133, 211
BssNI GRCGYC 1 cut(s) 100
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 1 cut(s) 154
BstACI GRCGYC 1 cut(s) 100
BstC8I GCNNGC 1 cut(s) 392
BstDEI CTNAG 5 cut(s) 161, 198, 278, 312, 395
BstF5I GGATG 2 cut(s) 232, 306
BstFNI CGCG 1 cut(s) 328
BstH2I RGCGCY 1 cut(s) 488
BstHHI GCGC 1 cut(s) 487
BstKTI GATC 2 cut(s) 136, 214
BstMAI GTCTC 1 cut(s) 554
BstMBI GATC 2 cut(s) 133, 211
BstMWI GCNNNNNNNGC 1 cut(s) 102
BstNI CCWGG 1 cut(s) 263
BstSCI CCNGG 1 cut(s) 261
BstSFI CTRYAG 1 cut(s) 114
BstUI CGCG 1 cut(s) 328
BstV2I GAAGAC 1 cut(s) 543
Bsu36I CCTNAGG 2 cut(s) 198, 312
BtgZI GCGATG 1 cut(s) 515
BtsCI GGATG 2 cut(s) 232, 306
Cac8I GCNNGC 1 cut(s) 392
CfoI GCGC 1 cut(s) 487
Cfr13I GGNCC 1 cut(s) 268
Csp6I GTAC 2 cut(s) 45, 319
CviAII CATG 1 cut(s) 418
CviJI RGCY 9 cut(s) 85, 145, 325, 339, 394, 402, 423, 474, 506
CviKI_1 RGCY 9 cut(s) 85, 145, 325, 339, 394, 402, 423, 474, 506
CviQI GTAC 2 cut(s) 45, 319
DdeI CTNAG 5 cut(s) 161, 198, 278, 312, 395
DpnI GATC 2 cut(s) 135, 213
DpnII GATC 2 cut(s) 133, 211
Eco32I GATATC 1 cut(s) 516
Eco47I GGWCC 1 cut(s) 268
Eco47III AGCGCT 1 cut(s) 486
Eco57I CTGAAG 1 cut(s) 107
Eco81I CCTNAGG 2 cut(s) 198, 312
EcoRI GAATTC 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 261
EcoRV GATATC 1 cut(s) 516
EcoT22I ATGCAT 1 cut(s) 471
FaeI CATG 1 cut(s) 421
FaqI GGGAC 1 cut(s) 178
FatI CATG 1 cut(s) 417
FbaI TGATCA 1 cut(s) 211
Fnu4HI GCNGC 1 cut(s) 326
FokI GGATG 2 cut(s) 239, 293
Fsp4HI GCNGC 1 cut(s) 326
FspBI CTAG 3 cut(s) 41, 533, 575
GlaI GCGC 1 cut(s) 486
GluI GCNGC 1 cut(s) 326
GsuI CTGGAG 1 cut(s) 284
HaeII RGCGCY 1 cut(s) 488
HhaI GCGC 1 cut(s) 487
Hin1I GRCGYC 1 cut(s) 100
Hin1II CATG 1 cut(s) 421
Hin6I GCGC 1 cut(s) 485
HinP1I GCGC 1 cut(s) 485
HinfI GANTC 3 cut(s) 191, 509, 562
HphI GGTGA 2 cut(s) 91, 426
Hpy166II GTNNAC 1 cut(s) 157
Hpy188I TCNGA 1 cut(s) 133
Hpy188III TCNNGA 4 cut(s) 257, 365, 553, 568
Hpy8I GTNNAC 1 cut(s) 157
Hpy99I CGWCG 1 cut(s) 105
HpyAV CCTTC 2 cut(s) 70, 85
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 1 cut(s) 100
HpyCH4V TGCA 2 cut(s) 179, 469
HpyF10VI GCNNNNNNNGC 1 cut(s) 102
HpyF3I CTNAG 5 cut(s) 161, 198, 278, 312, 395
HpySE526I ACGT 1 cut(s) 100
Hsp92I GRCGYC 1 cut(s) 100
Hsp92II CATG 1 cut(s) 421
HspAI GCGC 1 cut(s) 485
Ksp22I TGATCA 1 cut(s) 211
Kzo9I GATC 2 cut(s) 133, 211
LweI GCATC 2 cut(s) 166, 315
MaeI CTAG 3 cut(s) 41, 533, 575
MaeII ACGT 1 cut(s) 100
MaeIII GTNAC 1 cut(s) 329
MalI GATC 2 cut(s) 135, 213
MboI GATC 2 cut(s) 133, 211
MboII GAAGA 2 cut(s) 100, 548
MluCI AATT 2 cut(s) 71, 548
MlyI GAGTC 2 cut(s) 200, 556
Mph1103I ATGCAT 1 cut(s) 471
MseI TTAA 4 cut(s) 149, 243, 492, 580
MspR9I CCNGG 1 cut(s) 263
MvaI CCWGG 1 cut(s) 263
MvnI CGCG 1 cut(s) 328
MwoI GCNNNNNNNGC 1 cut(s) 102
NdeII GATC 2 cut(s) 133, 211
NlaIII CATG 1 cut(s) 421
NlaIV GGNNCC 2 cut(s) 355, 453
NmuCI GTSAC 1 cut(s) 329
NsiI ATGCAT 1 cut(s) 471
PfeI GAWTC 1 cut(s) 509
PkrI GCNGC 1 cut(s) 327
PleI GAGTC 2 cut(s) 199, 556
PpsI GAGTC 2 cut(s) 199, 556
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 355, 453
PspPI GGNCC 1 cut(s) 268
RsaI GTAC 2 cut(s) 46, 320
RsaNI GTAC 2 cut(s) 45, 319
SaqAI TTAA 4 cut(s) 149, 243, 492, 580
SatI GCNGC 1 cut(s) 326
Sau3AI GATC 2 cut(s) 133, 211
Sau96I GGNCC 1 cut(s) 268
SchI GAGTC 2 cut(s) 200, 556
ScrFI CCNGG 1 cut(s) 263
SfaNI GCATC 2 cut(s) 166, 315
SfcI CTRYAG 1 cut(s) 114
SinI GGWCC 1 cut(s) 268
SmlI CTYRAG 2 cut(s) 334, 566
SmoI CTYRAG 2 cut(s) 334, 566
Sse9I AATT 2 cut(s) 71, 548
SsiI CCGC 2 cut(s) 119, 326
SspI AATATT 1 cut(s) 218
SspMI CTAG 3 cut(s) 41, 533, 575
StyD4I CCNGG 1 cut(s) 261
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 1 cut(s) 103
TaqI TCGA 1 cut(s) 512
TasI AATT 2 cut(s) 71, 548
TauI GCSGC 1 cut(s) 328
TfiI GAWTC 1 cut(s) 509
Tru1I TTAA 4 cut(s) 149, 243, 492, 580
Tru9I TTAA 4 cut(s) 149, 243, 492, 580
TseFI GTSAC 1 cut(s) 329
Tsp45I GTSAC 1 cut(s) 329
TspDTI ATGAA 3 cut(s) 195, 243, 561
VpaK11BI GGWCC 1 cut(s) 268
XapI RAATTY 1 cut(s) 548
XspI CTAG 3 cut(s) 41, 533, 575
ZraI GACGTC 1 cut(s) 101
Zsp2I ATGCAT 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.